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Citations for Millipore Sigma :
451 - 500 of 4079 citations for Recombinant Human B4GALT1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Molecular Biology 2020Quote: ... and FLAG-tagged ribosomes were purified using M2 FLAG-affinity resin (Sigma), as described previously (Inada et al. ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Genetics 2020Quote: ... anti-mouse secondary antibody tagged with alkaline phosphatase (Sigma, catalogue no: A3562), CDP star (sigma) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG-tagged TSC2 was immunoprecipitated with ANTI-FLAG® agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged proteins were recognized using a murine antiHA antibody (Sigma-Aldrich) and then an antiMouse IgG-peroxydase antibody (KPL) ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tagged proteins was captured using IgG sepharose fast flow beads (Sigma) and proceeded as described (33) ...
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bioRxiv - Biochemistry 2019Quote: ... Strep-II-tagged proteins were bound to Strep-Tactin resin (EMD Millipore), washed with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged ZC3H11A was immunopurified with anti-Flag(M2) magnetic beads (Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-tagged anti-rabbit and anti-mouse secondary antibodies (SA) (Sigma-Aldrich). Densitometry values of the immunoblots were computed using GelQuant.Net software.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... HA tagged CPα was probed using a mouse anti-HA antibody (Sigma). Probed proteins were detected using donkey anti-rabbit IgG antibody ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).