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Citations for Millipore Sigma :
451 - 500 of 566 citations for PVPP Powder since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Nonspecific binding sites on the membrane were blocked by incubating the membrane in 5% milk powder in PBS containing 1X Tween-20 (Sigma). The membrane was then incubated with the primary antibody of interest at 40C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of each complex was mixed with 7 μl Orange G dye (65% w/v sucrose, 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.3% Orange G powder from Sigma Life Sciences), loaded onto a 1.0% agarose gel ...
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bioRxiv - Plant Biology 2022Quote: ... The time channel in the GC-MS spectrum for IAA was identified using a commercial IAA powder (from Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... followed by blocking for 1 hour at room temperature with 4% skim milk powder (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline pH 7.4 (TBS ...
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bioRxiv - Biochemistry 2019Quote: ... Then expression was induced by adding 10 ng/ml nisin (powder from Sigma-Aldrich dissolved in 50 mM lactic acid) and further grown for additional 3 h at 20 °C ...
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bioRxiv - Biophysics 2019Quote: Abiotic hydrogels were produced using alginic acid sodium salt powder or low-gelling temperature agarose (both purchased from Sigma-Aldrich).
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Pathology 2021Quote: ... ORO staining solution was prepared as follows: the stock solution was made of 0.5 g of ORO powder (O0625, Sigma, USA) dissolved in 100 ml of 100% isopropanol ...
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bioRxiv - Pathology 2020Quote: ... samples were stained with freshly prepared Oil Red O working solution (Oil Red O Powder from Sigma in 60% isopropanol) for 15 minutes ...
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bioRxiv - Genomics 2021Quote: ... fluorescent E-barcodes were defined by patterning the SU-8 structure doped by adding 0.004 wt‰ of Rhodamine 6G powder (Sigma-Aldrich) into SU-8 precursor.
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bioRxiv - Physiology 2021Quote: ... an isotonic solution containing luciferase-luciferin (LL) was prepared from a vial of ATP assay mix-lyophilized powder (Sigma-Aldrich) was used ...
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bioRxiv - Microbiology 2020Quote: ... we coated the well with 0.5 mg/ml Concanavalin A from Canavalia ensiformis (Jack bean) Type IV-S (lyophilized powder, aseptically processed, Sigma-Aldrich). Each well was loaded with 300 µL of Concanavalin A solution (dissolved in water ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked in 5% (w/v) non-fat dried milk powder in 1x Tris-buffered saline with 0.5% Tween20 (Sigma-Aldrich) for 1 h and then incubated in the appropriate primary antibody over night at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Tamoxifen solution (10-20 mg/mL) was prepared for intraperitoneal injections by dissolving the powder (T5648, Sigma, St Louis, MO) in a mix of 10% ethanol and 90% cornflower oil (C8267 ...
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bioRxiv - Cell Biology 2022Quote: ... diluted into DMEM/F12 media at a concentration of 1mg/ml with 10μM methacholine (dry powder dissolved in DMEM/F12, Sigma A2251). Imaging was conducted on the Olympus FVMPE-RS multiphoton laser scanning microscope equipped with a MaiTai HPDS-O IR pulsed laser (800 nm for FITC ...
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bioRxiv - Plant Biology 2022Quote: ... agar plates supplemented with 1% sucrose and 0.08% charcoal (as a powder to increase image background of etiolated plants, SIGMA-ALDRICH). For chemical treatments ...
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bioRxiv - Cancer Biology 2023Quote: ... was thawed at 4°C and supplemented with 10 U mL−1 horseradish peroxidase (type VI lyophilized powder, Sigma-Aldrich) and 0.4 μL mL−1 hydrogen peroxide solution (30% w/w ...
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bioRxiv - Pathology 2023Quote: ... Nuclear fast red solution was prepared using 0.1 g nuclear fast red powder in 100 mL distilled water with 5 g Al2(SO4)3 (Sigma-Aldrich). Deparaffinized slides were washed with distilled water and then incubated with the Alcian blue solution for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Poly D-lysine treatment was performed by coating plates with poly D-lysine hydrobromide (0.1 mg mL−1 in H2O from lyophilized powder, Sigma-Aldrich) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... primary and secondary antibody incubations were done in TBS-T (Roth, 1061.1) supplemented with 5% milk powder (Heirler, 4010318030305) or 5% BSA (Sigma, A7906). Blots were blocked for 1h at RT and primary antibody was added over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Phosphate buffer saline solution (Ca 2+, Mg 2+ free) was prepared using Phosphate Buffer Saline (PBS) powder (Sigma Aldrich, USA.). 9.6 grams of the powdered reagent was added to 990 milliliters of ultra-purified milli-Q water (Merck ...
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bioRxiv - Biophysics 2023Quote: ... a 10−3 M stock solution of TPPS4 was prepared by dissolving the powder (Sigma Aldrich, St. Louis, Missouri, USA) in distilled water ...
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bioRxiv - Biophysics 2022Quote: ... and 200 μl of (in 10 μg/mg mAb concentration) Papain (Papain from papaya Latex, 89% pure lyophilized powder from Sigma). The mixture was then kept in the incubator shaker at 37 degrees Celsius for 8-10 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... The lyophilized MFP ECM samples were ground into a powder and mixed into 0.1 M hydrochloric acid–pepsin solution (1 mg/mL) (Sigma-Aldrich) at a concentration of 20 mg/mL ...
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bioRxiv - Genomics 2024Quote: ... The lyophilized mycelium of each isolate was ground to a fine powder in a 2 mL microcentrifuge tube using stainless steel beads (2.8 mm diameter; Sigma-Aldrich) in a FastPrep-24 kit (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... were secured to the skull with Metabond and the surface covered with a layer of blackened Metabond (carbon powder, Sigma). Finally ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Microbiology 2023Quote: ... from a 50 mM biotin stock solution prepared in water with 60 mM NaOH (lyophilized biotin powder, 500 mg stock, Merck Life Science BV (Sigma)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed with TBS (50mM Tris pH 7.6, 150 mM NaCl) and blocked with 5% skim milk powder (Sigma Aldrich) in TBST (TBS with 0.05% Tween20 ...
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bioRxiv - Neuroscience 2024Quote: ... and the area between the craniotomy and the inner edge of the metal ring was covered with opaque dental cement (Metabond, Parkell, made opaque by adding 0.5 g of carbon powder, Sigma Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... blots were blocked in 5% (w/v) milk powder in PBS (PanReac AppliChem #A0965,9100)-TWEEN® 20 (Sigma-Aldrich #P1379) (PBS-T) ...
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bioRxiv - Neuroscience 2024Quote: ... in corn oil (Mazola, Memphis, TN) were prepared at a concentration of 9 mg/mL by dissolving deltamethrin powder in acetone (Sigma), mixing the acetone with corn oil ...
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bioRxiv - Biochemistry 2024Quote: ... powder was thawed in 25 mL of ribosome lysis buffer (1x Buffer A, 1 mg/mL heparin sodium salt (Sigma)) supplemented with 2 mM DTT ...
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bioRxiv - Cell Biology 2024Quote: ... After transfer the blots were blocked in a 5 %(w/v) solution of milk powder in TBS-T (50 mM Tris, 150 mM NaCl, 0.05% Tween 20 (Sigma, P9416)) for 1h at RT ...
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bioRxiv - Plant Biology 2020Quote: ... M82 and yft1 tomato seeds were sown separately on MS0 solid medium containing 4.41 g/L MS powder (Sigma, CA, USA), 30.00 g/L sucrose ...
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bioRxiv - Bioengineering 2021Quote: ... Tissue specific hydrogels for muscle and tendon (mECM and t-ECM) were produced from the powder by digesting with 20:1 weight ratio of pepsin from porcine submucosa (Sigma-Aldrich) in .01 M HCl at a concentration of 10mg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... the stock thrombin solution was prepared by dissolving bovine thrombin protein powder isolated from bovine plasma (cat. no. T4648, Sigma-Aldrich) in DPBS ...
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bioRxiv - Microbiology 2020Quote: ... 100 µL of cell-free spent culture media served as a crude enzyme source was mixed with 100 µL of 1% (w/v) chitin powder (Sigma, USA) in 50 mM sodium phosphate buffer (pH 6.5) ...
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bioRxiv - Microbiology 2021Quote: ... Then the pellet was resuspended in 1 mL 100 mM sodium acetate (pH 4.5) supplemented with amyloglucosidase powder (Sigma-Aldrich, 10115) at a final concentration of 35 U/mL ...
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bioRxiv - Immunology 2022Quote: ... After 3 days incubation at 37°C the overlay was removed and the plates stained with crystal violet solution (spatula tip (∼4 mg) crystal violet powder (Sigma Aldrich) solved in 30 ml 37% formalin and 120 mL PBS (Sigma Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... participants were administered a dosage of 2 g tyrosine (supplied by The Hut.com Ltd.) or placebo (2 g cellulose powder (Sigma-Aldrich Co.), solved in 200 ml orange juice (Solevita ...
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bioRxiv - Neuroscience 2022Quote: ... and guide cannulas were fixed in position using dental cement (Provinice; Shofu, Inc., Japan) mixed with 5% carbon powder (484164; Sigma, USA). Mice were allowed to recover from surgery for 4 weeks in their home cages before behavioral experiments were initiated.
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated in TBST-5 % w/v milk (TBST supplemented with 5 % (w/v) skimmed milk powder) with anti-rabbit-HRP secondary antibody (A0545, Sigma Aldrich) (1:5,000 ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
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bioRxiv - Genomics 2019Quote: Aphid samples were ground to a fine powder and total RNA was extracted using a plant RNA extraction kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... A stock ECM digest concentration of 10 mg/mL was prepared by adding 200 mg of ECM powder to a 20 mL solution of pepsin (Sigma P7012) at a concentration of 1 mg/mL (≥2,500 units/mg ...
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bioRxiv - Bioengineering 2019Quote: ... was incubated in 5% milk powder in TBS-t buffer with anti-tubulin primary antibody (1:500, Sigma #T9026, Buchs, Switzerland)) ...
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bioRxiv - Bioengineering 2020Quote: ... 950 g of distilled water and 24.81 g of Sigma methylcellulose powder (Cat # M0512, 4,000 cP, Sigma-Aldrich, St. Louis, MO) were autoclaved separately at 121°C for one hour ...
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bioRxiv - Immunology 2021Quote: ... The plates were incubated for 3 days at 37°C and the cytopathic effect determined by staining with 100 µl/well crystal violet solution (spatula tip (∼4 mg) crystal violet powder (Sigma Aldrich) solved in 30 ml 37% formalin and 120 ml PBS (Sigma Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... Then centrifuged for 5 minutes at 800 g at 5°C and the pellet was resuspended in 500 μl sorting buffer (1 % BSA wt/vol, 2 % skimmed milk powder (Sigma Aldrich), 1 mM EDTA in PBS pH 7.4 ...