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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Macrophage metalloelastase MMP12 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... THP-1 cells were differentiated to macrophages using 100 nM phorbol 12-myristate 13-acetate (PMA, Sigma P8139), the PMA was also included in downstream experimental design ...
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bioRxiv - Microbiology 2023Quote: ... 20% L-cell conditioned medium (containing macrophage colony-stimulating factor) and 100 U/ ml of penicillin-streptomycin (Sigma) (complete RPMI media) ...
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bioRxiv - Microbiology 2023Quote: ... U937 were differentiated into macrophage-like cells in 20 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) for 72 hours ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were activated to macrophages by adding 30ng/mL phorbol-12-myristate-3-acetate (PMA; Sigma-Aldrich: P8139) for 24hr ...
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bioRxiv - Neuroscience 2023Quote: hiPSC-derived macrophages and microglia were detached using a solution of 4 mg/ml Lidocaine hydrochloride monohydrate (Sigma) and 5mM EDTA (ThermoFisher scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Microbiology 2019Quote: ... Further confirmation of phagocytic activity of macrophages was conducted by actin inhibition (cytochalasin D; C8273, Sigma, USA; 1 h) before inoculation.
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bioRxiv - Microbiology 2020Quote: ... For obtaining macrophage monolayer THP1 cells with required cell density were treated with 20ng/ml of PMA (P8139, Sigma) and after one day PMA was removed with 2 washes of RPMI.
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bioRxiv - Bioengineering 2020Quote: ... THP-1 cells were differentiated into macrophage-like cells with 150 nM phorbol 12-myristate 13-acetate (PMA) (Sigma) before the experiment.
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bioRxiv - Microbiology 2020Quote: ... part of the macrophages was lysed using deionized water containing 1% (vol/vol) Triton X-100 (Sigma Aldrich, USA) for 15 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of mycobacteria isolated from axenic culture or from infection of macrophages were filtered thourgh a 0.5 μm pore size PVDF filter (Millipore) to remove cell clumps and enrich single cells ...
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bioRxiv - Microbiology 2019Quote: ... At 5h post-infection DMEM media was removed and infected macrophages treated for 15 mins with 0.1% Triton-X-100 (Sigma). Intracellular survival of P ...
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bioRxiv - Immunology 2020Quote: Human monocyte-derived macrophages (hMDMs) were differentiated from buffy cones from healthy donors using gradient separation (Histopaque 1077, Sigma). Following Histopaque separation ...
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bioRxiv - Molecular Biology 2021Quote: ... THP-1-derived macrophages were then stimulated for 24 hours with 1 μg/mL LPS (Sigma, St. Louis, MO), followed by a 24-hour incubation with 1×1010 particles/mL Exo-101 ...
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bioRxiv - Immunology 2020Quote: ... after which medium was aspirated and cells were incubated in 250μl cell culture medium with 250μl Omentum factors or macrophage serum free media with ATRA (Sigma, 1μm) or without for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... 1 mL of lentiviral supernatant was added to 5×105 RAW 264.7 macrophages with 4 μg/mL of hexadimethrine bromide (Millipore) and cells were spinoculated at 300 x g for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... THP1 monocytes were differentiated to macrophages by treatment with 20 ng/ml phorbol myristate acetate (PMA) (Sigma-Aldrich P8139) for 20 hours followed by incubation in PMA free RPMI 1640 media for two days and used for infections ...
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bioRxiv - Immunology 2022Quote: The RAW 264.7 macrophage cell line was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich, D6546) with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: ... in alveolar macrophage media (1X penicillin-streptomycin [Gibco, MA], 10% FBS [Gibco, MA]/0.1%β-mercaptoethanol [Sigma-Aldrich, MA]). After a 2 hour incubation at 37°C ...