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Citations for Millipore Sigma :
451 - 500 of 1510 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The supernatant (S1) was added gently on 3% Percoll-sucrose (Percoll, P1644, Sigma Aldrich, UK) and centrifuged at 20,000 rpm for 8 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The cells were maintained in DMEM supplemented with 10% FCS for three days and then incubated with DMEM supplemented with 10% FCS and 5 μg/ml puromycin (Sigma-Aldrich) for four days to allow the selection of cells expressing the gene of interest (40).
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Bioengineering 2024Quote: One gram of mucin glycoprotein from bovine submaxillary gland was treated with 1U neuraminidase from Vibrio cholerae (Sigma-Aldrich, Saint Louis, MO, USA) in sodium acetate buffer 0.05 M (pH 5 ...
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bioRxiv - Cell Biology 2020Quote: ... and Hek293 (ATCC CRL-1573, embryonic kidney) were cultured in DMEM high glucose (Sigma-Aldrich), all supplemented with 2mM glutamine (Biochrome) ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293-GNTI cells were transfected with 1 mg DNA and 3 mg PEI (Sigma-Aldrich) per liter of cells at a density of 1.3-1.8E6 cells/ml ...
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bioRxiv - Cell Biology 2022Quote: SLC1A2/A3-expressing HEK293 Flip-In cells were plated on Poly-L-lysine hydrobromide (Sigma)-coated glass coverslips and induced with doxycycline (1 μg/ml ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... HEK293 and HZ1 cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC number CRL-1573) were cultured in Dulbecco’s Modified Eagle’s medium (DEME) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... SH-SY5Y and HEK293 cells were maintained in Dulbecco’s Modified Eagles Medium (DMEM; Sigma Aldrich) with 10% and 5% FBS respectively ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HSV tag (Sigma-Aldrich H6030, 1:1,000 or 1:2,000), Rpb1-Core (Y80 Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag (M2) and α-tubulin (T5168) from Sigma-Aldrich; βIII-tubulin from Biolegend (801201).
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag was cleaved with restriction-grade thrombin (Millipore) overnight on ice in Tris buffer supplemented with 2.5 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... The HA tag oligonucleotide strand was synthetized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 5) ...
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bioRxiv - Microbiology 2019Quote: HA Tag-FITC (7 ug/mL) (Sigma, clone HA-7)
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG tag (clone M2-F1804, Sigma, 1:1000), mouse anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Biophysics 2021Quote: ... The MBP tag was cleaved with Thrombin (Sigma-Aldrich, USA) at 0.4 unit per mg of protein ...
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bioRxiv - Cell Biology 2022Quote: ... then HRP tags were activated with enhanced chemiluminescence solution (Millipore). Signal was detected and imaged via ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... anti-Flag or anti-Pk (V5 Tag) antibodies (Sigma-aldrich) were used for detecting epitope-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... which carries the N and C-terminal 6xHistidine tags (Novagen), using the Hi-Fi DNA assembly kit (New England Biosciences).
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bioRxiv - Cell Biology 2022Quote: TAG quantification was performed using Triglyceride Quantification kit (Sigma-Aldrich). For TAG quantification of whole body ...
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bioRxiv - Microbiology 2023Quote: ... An anti-histidine tag antibody (Sigma, St. Louis, MO, USA) and serum from the immunized mice were used as positive controls ...
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bioRxiv - Microbiology 2023Quote: ... or with rabbit primary antibodies against HA-tag (Sigma-Aldrich) followed by anti-rabbit HRP-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal against FLAG tag at 1:500 (F7425, Millipore), rabbit polyclonal against Myc tag at 1:2000 (ab9106 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Cell Biology 2023Quote: ... human ephrin-B2-Fc (10 µg/ml, RnD Systems, #7397-EB) was mixed with Cy3-conjucated Fc-antibody (5 µg/ml, Sigma-Aldrich, # C2571) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... 1:2000 (SARS-1-S, MERS-S and SARS-2-S) or 1:5000 (229E-S)] and mouse anti-β-actin (Sigma-Aldrich, A5447, 1:5000). As secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... each comprising of 53 15-mer peptides overlapping by 11 residues, were purchased from Mimotopes Pte Ltd (Clayton, Victoria, Australia) and reconstituted in DMSO (Sigma-Aldrich, St. Louis, Missouri, USA). Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... S1-containig cell culture media was incubated with 10 μL of anti-Flag beads (Sigma, F2426) to precipitate S1 subunit ...
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bioRxiv - Neuroscience 2023Quote: ... Total supernatant (S1 + S2) was passed through a 10 μm hydrophobic PTFE filter (LCWP02500, Merck Millipore) and centrifuged for 1 min at 4,000 g ...
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bioRxiv - Cancer Biology 2021Quote: ... complemented with 20% fetal calf serum (FCS) (Sigma-Aldrich), 1% pen/strep (Gibco ...
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bioRxiv - Immunology 2021Quote: ... with 2% foetal calf serum (FCS, Sigma-Aldrich, UK). Lymphoprep (d = 1.077 g mL−1 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524) at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma Aldrich), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... Media was also supplemented with 10% dialysed FCS (Sigma), penicillin (100 IU/mL) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 10% fetal calf serum (FCS; Sigma, Vienna, Austria), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: ... supplement with 10% fetal calf serum (FCS, Sigma, US) at 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich), and penicillin–streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... and fluorinated oil (Fluorinert FC-40) (Sigma Aldrich, F9755) treated media (Figure 3a ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10% FCS and 1% GPS solution (Sigma-Aldrich) after ZF probe administration at 5 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Fusicoccin (FC, 1mM stock in 96% ethanol, Sigma Aldrich), 5-Adamantyl-IAA (pico-cvxIAA ...