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Citations for Millipore Sigma :
451 - 500 of 7132 citations for Japanese Encephalitis Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 or PC-3 cells were transduced with virus-containing culture media supplemented with 10 µg/ml Polybrene (Millipore, Cat #TR-1003-G) for 24 hours and replaced with fresh growth media ...
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bioRxiv - Microbiology 2022Quote: ... variant was obtained from the RIVM (Netherlands) through European Virus Archive global and expanded on VeroE6 cells grown in DMEM (Sigma Aldrich, Cat #D6429) supplemented with 4% of FCS (Gibco ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Near-confluent cells were transduced by aspirating the medium and adding 1ml of the respective virus diluted in 1ml of EGM2 with 4mg/ml polybrene (Sigma, Prod. No. H9268-5G). Cells were then left to incubate for 6h at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Wild-type KP4 cells were transduced with concentrated virus and GFP-expressing cells were selected using puromycin selection (1 μg/mL, Sigma-Aldrich, St Louis, USA). GFP-expressing PDOs were created using the same virus as KP4 but with a slightly different transduction protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were further incubated in medium containing 300 nM tamoxifen for 48 h and then incubated with two-fold dilutions of AdV-C5-EGFP stock virus in DMEM medium (Sigma-Aldrich, cat. no. D6429) supplemented with 7.5% fetal calf serum (Gibco/ Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: MEF cells were plated in 10 cm dishes to give a 70% confluency the following day for viral transduction by the addition of 5 ml of virus with 5 ml of fresh medium and 10 μg/ml Polybrene (Sigma-Aldrich, TR-1003-G). After 24h incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... RMG-1 ARID1A-WT and ARID1A-KO cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...