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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The protein was separated by 8%–12% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Millipore). Membranes were blocked in 5% BSA in TBS for 1 hour at room temperature and then incubated with primary antibodies overnight at 4 ℃ ...
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bioRxiv - Neuroscience 2021Quote: ... frozen longitudinal sections of the whole optic nerve were processed with GAP-43 monoclonal antibody (1:500; Chemicon Millipore) in order to stain regenerating axons ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Cancer Biology 2022Quote: ... PBS and 1 μg/mL Human Plasma Fibronectin Purified Protein (Sigma-Aldrich, Cat. #: FC010). 1N NaOH was added to achieve a neutral ∼pH and a thin base layer of 100 μL volume was added to the well of a 48-well tissue culture plate precoated with 1% agarose ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking of unspecific protein-antibody interactions was performed with 20% human AB serum (Sigma) in PBS for one hour at RT in the dark ...
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bioRxiv - Biochemistry 2024Quote: For rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) experiments TC28a2 human chondrocytes (Sigma) were transfected with human His-HA-RORβ (or pCMV6 base vector for control samples ...
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bioRxiv - Immunology 2020Quote: Bafilomycin A (HY-100558, MCE, Monmouth Junction, NJ, USA) was diluted in dimethyl sulfoxide (DMSO, Sigma-Aldrich, Merck) to 100 μM stock solution and diluted to a 20 nM working solution ...
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bioRxiv - Cell Biology 2024Quote: ... we utilized a commercial membrane protein extraction kit - The ProteoExtract® Native Membrane Protein Extraction Kit (EMD Millipore). This extraction method involves sequential lysis steps ...
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bioRxiv - Cancer Biology 2022Quote: was measured using the Cell Counting Kit-8 (Sigma-Aldrich, Cat# 96992-500) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: Cell viability was determined using Cell Counting Kit 8 (Sigma, Cat. no. 96992), which selectively stains viable cells.
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bioRxiv - Molecular Biology 2024Quote: ... Viability was assessed by a colorimetric assay (Cell Counting Kit-8, Sigma Aldrich), as per the manufactureŕs instructions ...
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bioRxiv - Genetics 2022Quote: ... the membrane was probed with the primary antibody overnight at 4°C (rabbit anti-beta galactosidase antibody, 15518-1-AP Protein Tech, dilution 1/1000: mouse anti-alpha-tubulin, T6199 Sigma-Aldrich, dilution 1/10 000) and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
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bioRxiv - Genetics 2021Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
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bioRxiv - Microbiology 2021Quote: ... the antibodies isotypes were determined by ELISA using a mouse Ig isotyping kit (ISO-2, SIGMA).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 125 μl of lysis buffer provided with a CAT ELISA kit (Sigma). CAT expression and Renilla luciferase activities were measured and the ratio was calculated.
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Cancer Biology 2024Quote: ... the ProteoExtract Protein Precipitation Kit (Sigma Millipore) was used with the following modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... the ProteoExtract Protein Precipitation Kit (Sigma Millipore) was used with the following modifications ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Immunology 2023Quote: ... 100,000 T84 cells per well were seeded on glass bottom 8-well chamber slides coated with 2.5% human collagen (Sigma #C5533-5MG) diluted in water ...
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bioRxiv - Molecular Biology 2022Quote: ... MAX Efficiency buffer and 15 µg linearized plasmid DNA was added before loading into an 0.2mm gap electroporation cuvette (Sigma Z706086).
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bioRxiv - Molecular Biology 2023Quote: The substrates for PolA synthesis experiments were done by annealing three (gap substrate) or two (primer extension substrate) HPLC purified oligos (Sigma) in a thermocycler ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were prepared in two volumes (approx.8 ml) of CelLyticP buffer (Sigma-Aldrich, Cat No C2360) with 1 % of a Protease Inhibitor EDTA-free tablet (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µg retina protein extracts were separated on 8% polyacrylamide gels and blotted onto PVDF transfer membranes (Millipore) followed by blocking with non-fat dried milk (Applichem) ...
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bioRxiv - Physiology 2021Quote: ... Proteins were resolved by SDS-PAGE on 8% acrylamide gels and electrotransferred onto Immobilon P (Millipore, Bedford, MA). Then ...
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bioRxiv - Cell Biology 2023Quote: ... Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore; 05317) using a wet blot transfer system (BioRad) ...
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bioRxiv - Microbiology 2024Quote: ... The high-purity fusion protein was concentrated to 8 mg mL-1 using an ultrafiltration device (Millipore, Germany) and incubated with equimolar NE at a ratio of 1.2:1 for 0.5 h ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Immunology 2020Quote: ELISA plates (Cat. M5785, Sigma Aldrich) were first coated overnight at 4°C with 10μg/ml of each B cell peptide epitope ...
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bioRxiv - Immunology 2022Quote: – Antibody enzyme conjugates for ELISA (Sigma): Peroxidase conjugated Anti-mouse polyvalent immunoglobulins (IgG ...