Labshake search
Citations for Millipore Sigma :
451 - 500 of 10000+ citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
-
bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
-
bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
-
bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
-
bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
-
bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
-
bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
-
bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
-
bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
-
bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
-
bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
-
bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
-
bioRxiv - Molecular Biology 2020Quote: The purified LdCsm complex (100 μl) was first mixed with l00 μl Trizol agent (Sigma), and then 200 μl chloroform:isoamylalcohol (24:1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... 15 mM EDTA and 10% glycerol) supplied with 8 mM vanadyl ribonucleoside complex (Sigma-Aldrich), 50 U/ml SUPERase-In (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2020Quote: ... The ABC Peroxidase complex was visualized using 3,3’-Diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma Aldrich, D5637) for 5 minutes at RT ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
-
bioRxiv - Cancer Biology 2020Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich) and one drop of cross-linking modulator (2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane ...
-
bioRxiv - Biochemistry 2020Quote: ... The complex was then concentrated to 3-6 mg/ml (Amicon 100 kDa cutoff, Millipore) and loaded onto a size-exclusion column equilibrated with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... mPSF:PAP complex was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
-
bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.5 M NaCl with 300 μl of 1 M dimethylamine borane complex (ABC; Sigma-Aldrich) and 600 μl of 1 M formaldehyde for 2 h at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... was used at 1 µM and ARP 2/3 complex inhibitor - CK666 (182515; Sigma-Aldrich) was used at 100 µM concentration ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fractions containing intact complex were concentrated using an Amicon 100,000 MWCO centrifugal filter unit (Millipore). The concentrated sample was applied to a Superose 6 Increase 10/300 GL (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... the SEC-purified CerS6-Nb22 complex was incubated with 120 µM fumonisin B1 (Sigma-Aldrich) for 10 minutes on ice prior to protein concentration and subsequently for an additional 2h on ice prior to grid preparation ...
-
bioRxiv - Biochemistry 2024Quote: ... The purified complex was concentrated using a 10-kDa molecular mass cutoff Centricon tube (Millipore) to a final concentration of 6 mg/ml for crystallization trials ...
-
bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
-
bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
-
bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
-
bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
-
bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
-
YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
-
bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
-
bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
-
bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
-
bioRxiv - Cell Biology 2021Quote: Cells were seeded onto glass coverslips coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation) at a density of 80,000 cells and 40,000 cells for the PDGFRα homodimer cell line and PDGFRβ homodimer cell line ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
-
bioRxiv - Developmental Biology 2020Quote: Cells were seeded onto glass coverslips coated with 5 μg/mL human plasma fibronectin purified protein (Millipore Sigma #FC010). At ~90–100% confluency ...
-
bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
-
bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
-
bioRxiv - Neuroscience 2023Quote: Protein lysates were collected from primary human and murine spinal cord astrocytes in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich) ...