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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for E3 Ubiquitin Protein Ligase DTX1 DTX1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich, Cat #F1804) or 8 μg anti-U2AF2 antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA sonication and immunoprecipitation of the protein/DNA complexes with specific antibodies (anti-Flag M2, Sigma) or without antibodies as a negative control ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: rabbit anti-prosurfactant protein C (AB3786, EMD Millipore, Burlington, MA; 1:1000), rabbit anti-keratin 14 (KRT14 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... The pre-cleared protein solution was incubated with 3 μg anti-FLAG antibody (Sigma Aldrich, #F1804) or IgG (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse monoclonal anti-glial fibrillary acidic protein antibody (G3893, Sigma-Aldrich, St Louis, MO, USA). Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The precleared lysate was then transferred to Protein G Dynabeads coupled with anti-FLAG antibody (Sigma) and the mixture was incubated for 2 hours at 5°C on a roller ...
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bioRxiv - Immunology 2022Quote: ... coupled to protein A Sepharose magnetic beads (Ademtech 0423) and anti-TAX1BP1 antibody HPA024432 (Sigma-Aldrich) together with protein A Sepharose (GE Healthcare) ...
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bioRxiv - Genomics 2020Quote: ... except for the following modifications: immunoprecipitation was carried out using monoclonal antibodies against protein A (Sigma); the lysis buffer contained 10mg/ml heparin (sigma H7405) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Physiology 2022Quote: ... protein G beads were coupled to the pre-pro-vasopressin antibody or normal rabbit serum (Sigma) at a 1:50 ratio for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... The immunoprecipitation was performed by incubating the protein lysate with 1ug of GluA1 antibody (Millipore, ab1504) or 1ug HA antibody (Santa Cruz ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Puromycin incorporation into neosynthesized proteins was assessd with Western blotting and an anti-puromycin antibody (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449), secondary antibody (Biorad #170-6515) ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using chemiluminescence from horseradish peroxidase-coupled secondary antibodies Anti-rabbit HRP (Sigma; A0545), 1:15,000.
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bioRxiv - Bioengineering 2022Quote: ... Protein bound to the wells were detected with a peroxidase conjugated anti-Flag antibody (Sigma A8592) and quantified with QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™ 15169) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Cell Biology 2022Quote: ... the Ligase was diluted 1:40 in 1x Ligation Buffer (Sigma Aldrich, cat. no. DUO92008) and incubated in a humidity chamber for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... removing SNAP and inserting the commercially obtained gene via ligation with T4 ligase (Sigma, 10481220001) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Physiology 2021Quote: ... 500 μg of lysates/mouse were then immunoprecipitated with anti-PKG antibody(19) and protein conjugated-Sepharose beads and blotted with anti-NT antibody (Millipore, Cat#05-233, 1:2000). The same membrane was also blotted with anti-PKG antibody as a loading control ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for immunofluorescence (IF) in this study: a monoclonal antibody (mAb) against tau protein (clone Tau-1; Millipore, Cat Number: MAB3420, RRID: AB_94855) diluted 1:1000 ...
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bioRxiv - Plant Biology 2020Quote: Proteins were resolved on SDS-PAGE followed by immunoblotting using α-HA high affinity antibody 3F10 (Sigma) and α-c-Myc Antibody (9E10) ...