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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Cow Protein FAM210A FAM210A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation assay was carried out according to the Magna RIP RNA-Binding Protein Immunoprecipitation Kit manufacturer’s instruction (Millipore). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: Protein co-localization was assessed via proximity ligation assay (PLA) using the Duolink In Situ Red Starter Kit (Sigma) and PLA-approved primary antibodies for SC-35 (Abcam ...
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bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cell Biology 2022Quote: 96-well ELISA plates were coated overnight at 4°C with anti-mouse total β1-integrin (Millipore) or anti-mouse β1-integrin ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed five times with wash buffer (1 X PBS/0.05% Tween 20 (Sigma)) and blocked with 100 µl/well 5% FBS (Sigma) ...
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bioRxiv - Physiology 2021Quote: ... Total and high-molecular weight adiponectin was measured by ELISA (EZMADP-60K; EMD Millipore and MBS028367; MyBiosource).
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bioRxiv - Neuroscience 2023Quote: ... basal plasma (collection prior to IPGTT) leptin was measured using a leptin ELISA (Millipore Sigma; #EZRL-83K) following manufacturer’s instructions in duplicate ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...