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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Cell Biology 2023Quote: ... for 1.5 h at RT and incubated overnight at 4°C with primary antibodies: anti-dystrophin antibody (NCL-Dys1, 1:40, Novocastra Laboratories) and anti-alpha actinin antibody (A7732, 1:3000, Sigma-Aldrich). Membranes were incubated with the secondary antibody sheep anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Cancer Biology 2023Quote: ... AML cells were washed and cultured in Gartner’s medium (Alpha-MEM, 12.5% horse serum, 12.5% FCS, 1μM hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... alpha-cyclodextrin (MilliporeSigma, # C46421G), alpha-cyclodextrin sulfated sodium salt (MilliporeSigma, #4945425G), and hyaluronic acid sodium salt (MilliporeSigma, #53747) were purchased from Millipore Sigma.
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bioRxiv - Evolutionary Biology 2024Quote: ... they were incubated with an anti-acetylated alpha-tubulin mouse antibody (T6793, Sigma-Aldrich, St. Louis, MO, USA; 1:1000 dilution) in blocking buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... pLKO.1-puro-CMV-tGFP plasmids targeting SNX5 (5’-ACTATTACAATAGGATCAAAG-3’, 5’-CTGAGTATCTCGCTGTGTTTA-3’) and SNX6 (5’-AGTAAAGGATGTAGATGATTT-3’, 5’-GCCGAAACTTCCCAACAATTA-3’) (Sigma-Aldrich) were lentivirally transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Biophysics 2023Quote: ... 5 % D2O (Eurisotop) and 5 mM DSS-d6 (2,2-dimethyl-2-silapentane-5-sulfonate, Sigma) as internal reference (Wishart and Sykes ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Developmental Biology 2021Quote: ... vorticoides at different developmental stages were incubated in 10-5 M serotonin (5-HT) and 5-hydroxytryptophan (5-HTP) (Sigma-Aldrich, USA) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μM 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) was added into the culture medium for 10 h ...
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bioRxiv - Genomics 2020Quote: ... 5-aza-2’-deoxycytidine (5-aza-dC, Sigma, A3656) was diluted in DMSO at a concentration of 10mM and Abl.1 cells were treated using a concentration range of 10 nM to 20 μM 5-aza-2’-deoxycytidine ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM ammonium acetate pH 5 (Sigma-Aldrich 73594), 50% methanol (Honeywell LC230-4) ...
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bioRxiv - Genetics 2020Quote: For survival to 5-azacytidine (5-azaC, Sigma Aldrich), MG1655 and isogenic derivative (Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 nmol/L 3,3’,5-triiodo-L-thyronine (Sigma), 1 μmol/L dexamethasone (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μM S-(5’-Adenosyl)-L-methionine (Sigma A7007), 20 U SUPERase·In (Invitrogen AM2694) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol with addition of 5 mM desthiobiotin (Sigma).
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bioRxiv - Biochemistry 2022Quote: ... 5-(iodoacetamido)fluorescein (5-IAF) was from Sigma-Aldrich. SAM was purchased from New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mg/mL 5-FOA (5-Fluoroorotic acid, Sigma) was added to the selection plates ...
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bioRxiv - Biochemistry 2020Quote: ... The material was transferred into no-stick microtubes (Alpha Laboratories, cat. LW2410AS) and supplemented with 150 U/ml of Benzonase nuclease (Millipore, cat. 71206). Samples were incubated on a rotating wheel for 30 min at 4°C and centrifuged (16,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Alpha smooth-muscle actin (αSMA) and collagen I (col1) were stained using monoclonal antibodies (1:1000 diluted, Sigma, A2547 and SAB4200678, respectively). For detection of primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
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bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... C4-12 cells were cultured in phenol free minimum essential medium (MEM, Fisher #A10488-01) alpha media supplemented with 10% charcoal stripped serum (CSS; Sigma-Aldrich #F6765). Cell lines are maintained at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 30 uM of 5-hydroxytryptamine hydrochloride (5-HT) (Millipore Sigma) was applied into DIV5 cortical neuronal and cortical mixed cultures daily ...
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bioRxiv - Genomics 2020Quote: ... we added 10uM 5-Azacytidine (5-Aza, Sigma-Aldrich #A2385) to induce differentiation ...
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bioRxiv - Cancer Biology 2021Quote: 5-Fluorouracil (5-FU) (F6627, Millipore Sigma, St. Louis, MO) was administered in sterile 0.9% NaCl by intraperitoneal injections (i.p. ...
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bioRxiv - Immunology 2022Quote: ... injection of 150 µg 5-fluorouracil (5-FU, Sigma-Aldrich) per kg of body weight [26 ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM MgCl2 and 5 KU Benzonase nuclease (Merck Millipore) for 30 mins at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-Bromouridine 5′-triphosphate (BrUTP) were purchased from Sigma Aldrich.
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bioRxiv - Biochemistry 2021Quote: ... Bioconjugation of 5-hydroxytryptophan with 5-aminofluorescein (FLA) (Sigma-Aldrich) was carried out as reported [21] with several adjustments ...
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bioRxiv - Neuroscience 2022Quote: ... 5-min pulses of 50 μM 5-HT (Sigma, H9523) with a uniform interstimulus interval (ISI ...
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bioRxiv - Immunology 2023Quote: ... β2-mercaptoethanol (5×10−5 M; Sigma-Aldrich, MO, USA), and 10% heat inactivated foetal calf serum (FCS ...
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bioRxiv - Neuroscience 2024Quote: ... 5 glucose or 5 sodium D-/L-β-hydroxybutyrate (Sigma) or 1.25 sodium lactate (Sigma ...
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bioRxiv - Cell Biology 2024Quote: The sample was incubated overnight (12Lhours) at 4 °C with a primary mouse anti alpha- and beta-tubulin IgG antibodies (Sigma-Aldrich (REF: T5168) and Sigma-Aldrich (REF ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in PBS with 2% BSA containing primary antibodies against vimentin (Chicken, Encor CPCA-Vim, 1:1000) and alpha-tubulin (Mouse, Sigma T6199, 1:1000) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 5-Fluorouracil (Sigma) was reconstituted in DMSO (100 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... 5% BSA (Sigma) in PBS was injected into the tail vein of mice at 10 µL/g body weight ...
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bioRxiv - Neuroscience 2021Quote: ... 5% FBS (Sigma), penicillin ...