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Citations for SignalChem :
1 - 50 of 86 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 0.1 μg/ml MEK1 activated extracellular signal-regulated kinase 1 and 2 (pERK1/2) (SignalChem Biotech), 1 μM TTA-P2 (Alomone Labs) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein kinase activity of recombinant full-length AMPK (A1/B1/G1) enzyme (SignalChem, Richmond, BC Canada) was determined using a non-radioactive assay which measure changes in initial concentration of ATP via luminescence ...
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bioRxiv - Cancer Biology 2024Quote: ... Enzyme kinase activity was measure in the presence of 45 µM peptide substrate SAMStide (HMRSAMSGLHLVKRR) (SignalChem) in a buffered solution containing 5 mM MOP (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... MARK3 and 14-3-3ε were purchased (Active Motif, 81209, Signalchem, L02-11G, M45-10G, Y75-30H) and purity ...
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bioRxiv - Biochemistry 2024Quote: ... CK2 Substrate (synthetic peptide RRRADDSDDDDD) was purchased from SignalChem Biotech Inc ...
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bioRxiv - Cell Biology 2024Quote: ... were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem, Richmond, Canada) at 30°C for 30 min in a total volume of 30 μL kinase reaction buffer (5 mM MOPS ...
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bioRxiv - Molecular Biology 2024Quote: ... phosphorylation in real-time in the presence or absence of 1 µg Cdk11 (WT, S712E, or S712A)/Cyclin L and 0.5 µg CDK7/cyclin H (SignalChem Biotech Inc) using a LabChip EZ Reader microfluidic platform (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... and Cyclin L/CG16903 were synthetised by GeneArt and cloned into a proprietary N-terminal His tag vector for co-expression in Sf9 insect cells by SignalChem Biotech (Richmond ...
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bioRxiv - Systems Biology 2024Quote: ... Recombinant 2N4R Tau was purchased from SignalChem, and it was maintained in the manufacturer storage buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant 6xHis-PP1γWT or 6x His-PP1γT288A at a final concentration of 450 nM were incubated with increasing concentrations (50 nM, 100 nM, 200 nM) of 6xHis-PLK1 (SignalChem) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... 50 ng of CK1δ1-FL (from Signalchem) was incubated in kinase buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... 75 ng of purified CK1δ1-FL protein (from Signalchem) was incubated in kinase buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... were sourced commercially from SignalChem Biotech (Table S3) ...
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bioRxiv - Biochemistry 2024Quote: ... 400 μM CaCl2 (SignalChem catalog # C02-39B-500 calcium/Calmodulin solution) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant human AMPK (α1β1γ1 complex, SignalChem Biotech, P47-110GH) was diluted in enzyme dilution buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant human AMPKα1β1γ1 trimeric complex (SignalChem Biotech, P47-110GH) was diluted in enzyme dilution buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... or GST-ALK (SignalChem #A19-116) were incubated with GSH-Sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: Recombinant GST-tagged LTK (SignalChem #L11-11G) was incubated in the absence or in the presence of an equal amount of recombinant His-tagged IGF-1R (SignalChem #I02-11H ...
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bioRxiv - Neuroscience 2023Quote: ... Immobilized recombinant proteins were then incubated with 400 µl embryonic brain homogenates or with recombinant His-IGF-1R (SignalChem #I02-11H) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... and GST-LTK (SignalChem #L11-11G) or GST-ALK (SignalChem #A19-116 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 - 30 ng of GST-hDGKε (SignalChem, cat. No. D24–10G) was applied onto the gels ...
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bioRxiv - Neuroscience 2023Quote: ... was incubated in the absence or in the presence of an equal amount of recombinant His-tagged IGF-1R (SignalChem #I02-11H) in kinase buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant GST (SignalChem #G52-30U) and GST-LTK (SignalChem #L11-11G ...
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bioRxiv - Biochemistry 2023Quote: ... or Mer (SignalChem; Tyro3 (aa 455-end): T22-11G ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: PP2A dimer was purchased from SignalChem (PP2Aα/PPP2R1A Active Complex ...
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bioRxiv - Neuroscience 2023Quote: ... In vitro kinase assays were performed using purified recombinant Cpx proteins and the catalytic subunit of CK2 (C70-10G, SignalChem). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM ATP) and 2 ug of recombinant kinase (SignalChem; Tyro3 (aa 455-end): T22-11G ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated with recombinant human STK25 protein (Signalchem, S43-10G-10) (350 ng ...
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bioRxiv - Biochemistry 2023Quote: ... or recombinant human IKKβ protein (Signalchem, I03-10BG-10) in kinase reaction buffer in 96-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... and recombinant human STK25 protein (Signalchem, S43-10G-10) or recombinant human IKKβ protein (Signalchem ...
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bioRxiv - Neuroscience 2023Quote: ... Other human recombinant PKCs (SignalChem Biotech, Richmond, BC, Canada) were assayed in triplicate at the following concentrations ...
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bioRxiv - Neuroscience 2022Quote: ... Full-length recombinant Tau 1-441 phosphorylated in vitro by glycogen synthase kinase 3β (GSK3β) (SignalChem, Vancouver, BC, Canada) was used as the calibrator.
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bioRxiv - Cancer Biology 2022Quote: ... with 200 ng active ATR (Signalchem). Reactions were performed in 1× kinase buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... ATR protein was purchased from SignalChem (No. A27-35G). Anti-rabbit ATR antibody was purchased from Cell Signaling Technology (13934S) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
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bioRxiv - Biochemistry 2022Quote: ... RSK CTKD proteins (2 µM) were either untreated or pre-activated by ERK2 (0.1 µM) (Signalchem) in reaction buffer containing 200 µM ATP and 10 mM MgCl2 in kinase buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ng of PRKACA (SignalChem) was incubated with 5 µg of huntingtin in a buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... or ROCK2 enzyme (0.006 ng/mL; SignalChem, #R11-11H-10) was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 μg of Recombinant Active TBK1 (T02-10G-05, SignalChem), and 1 μL 10 mM ATP were combined in kinase reaction buffer (20 mmol/L Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... L747_A750>P (#E10-12MG, lot G1200-3), and L747_E749 (#E10-12LG, lot G1344-5) were purchased from SignalChem. The Promega ADP-Glo™ kinase assay kit was used to quantify the amount of ADP produced by each EGFR variant in 1XBFA buffer and in the presence or absence of erlotinib at varying concentrations ...
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bioRxiv - Cancer Biology 2022Quote: Human DARPP-32 isoforms purified from NSCLC cells were incubated with kinase-activated human IKKα protein (SignalChem) for in vitro kinase assays by following previously described methods70 ...
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bioRxiv - Pathology 2022Quote: ... The reaction was run in kinase buffer (Cat#K02– 09, SignalChem, Canada) for 30 min at 30°C and stopped using 6 × loading ...
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bioRxiv - Physiology 2022Quote: ... 0.1 mg/mL of BSA and 1× Assay Buffer I (SignalChem) were added to liposomes and 50 μL volumes were distributed across three Eppendorf tubes ...
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bioRxiv - Cancer Biology 2022Quote: ... CamKIIβ and CamKIV (150 ng, Signalchem) were incubated in reaction buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... coli according to the protocol described by SignalChem Lifesciences Corp. ...
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bioRxiv - Cell Biology 2022Quote: 0.5 μg of recombinant FLAG-CUL4A or FLAG-CUL4B (in complex with DDB1 and RBX1) or 0.15 μg native swine myelin basic protein (MBP; SignalChem) were added to 1x protein kinase buffer (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... PLK1 (SignalChem), Aurora A (SignalChem ...
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bioRxiv - Cell Biology 2022Quote: ... Aurora A (SignalChem) or Aurora B (SignalChem ...
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bioRxiv - Cell Biology 2022Quote: ... or Aurora B (SignalChem) kinases ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant Cyclin B1/Cdk1 (SignalChem Biotech Inc., Richmond, BC, Canada), and ADP-Glo kinase assay (Promega) ...