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Citations for Bethyl :
51 - 100 of 109 citations for SPP1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and an anti-human Mad2 rabbit antibody (1:1000 dillution) (Bethyl Laboratories, Inc.). The supernatant and precipitates of zebrafish oocyte extracts were separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2019Quote: Albumin secretion quantification was performed using the Human Albumin ELISA kit from Bethyl Laboratories (Montgomery ...
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bioRxiv - Microbiology 2019Quote: ... microtiter plates were coated with 1μg/well of anti-human IgG (Bethyl Laboratories) in 100μl carbonate/bicarbonate buffered solution (Sigma C3041 ...
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bioRxiv - Immunology 2021Quote: ... Goat anti-Human IgG-Fc fragment cross-adsorbed antibody HRP conjugated (Bethyl Laboratories) secondary at a 1:10,000 dilution for 1 hour at ambient temperature ...
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bioRxiv - Genetics 2020Quote: ... rabbit anti-human TGS1 (1:1500, Bethyl Laboratories Cat#A300-814A, lot 1), rabbit anti-GFP antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and 50 µl of Human IgG-Fc Fragment Antibody (Bethyl Laboratories, A80-104P) at 0.33 µg/mL were added to each well ...
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bioRxiv - Immunology 2019Quote: ... Dilutions of human reference serum (with IgG quantified at 4.4 mg/ml; Bethyl Laboratories) were made in blocking buffer (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The following antibodies were used to detect human proteins: TOP2α (Bethyl, Cat# A300-054A), TOP2β (Topogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Albumin secretion was quantified using the Human Albumin ELISA Quantitation Set (Bethyl Laboratories Inc) and CYP3A4 activity was determined using P450-Glo CYP3A4 Assay with Luciferin-IPA (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantification of secreted albumin was performed using a Human Albumin Quantification Kit (Bethyl Laboratories). Procedure according to manufacturer’s instructions and absorbance was measured using a microplate spectrophotometer at 450nm ...
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bioRxiv - Immunology 2020Quote: ... were coated with 1 µg/well goat anti-human IgG-Fc fragment antibody (Bethyl) in DPBS (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488 goat anti-rabbit IgG and Cy5 goat anti- human IgG (Bethyl); Alexa Fluor 350 goat anti-mouse and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... Blood was collected for quantification of human immunoglobulin kappa light chain by ELISA (Bethyl Laboratories). Animal handling and procedures were approved by the Norwegian food safety authority (FOTS10517).
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bioRxiv - Cell Biology 2020Quote: ... and probed with an anti-human Pum1 goat antibody (1:1000 dillution) (Bethyl Laboratories, Inc.), an anti-human Cyclin B1 rabbit antibody (1:100 dillution ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was detected with HRP-conjugated goat anti-human IgG Fc antibody (Bethyl Laboratories). Plates were washed five times with PBS-Tween between steps ...
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bioRxiv - Microbiology 2020Quote: ... Further confirmed by western blotting using Goat-anti human IgG conjugated with HRP (Bethyl, A80-119P). Soluble ACE 2 was purified using Nickel -NTA agarose beads (Qiagen Cat.No-1018244) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... reacted with HRP-conjugated anti-human IgG-Fc antibody or anti-mouse IgG-Fc antibody (Bethyl) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse plasma was analysed for human albumin using a commercial ELISA kit (Bethyl Laboratories, Texas, USA), following manufacturer’s instructions with the inclusion of positive and negative controls.
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bioRxiv - Immunology 2024Quote: ... or test reagents in basophil media including rabbit anti-human IgE (1 μg; obtained from Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... were coated with 200 ng of human FGF21 protein in 100 µL of coating buffer (E107, Bethyl) per well at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... at a 1:500 dilution or human IgG-heavy and light chain antibody (Bethyl Laboratories, A80-118A) at a 1:2000 dilution in 50 mM sodium carbonate (pH 9.6) ...
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bioRxiv - Immunology 2023Quote: Total IgD from cell culture supernatants was measured using the Human IgD ELISA Quantitation Set (Bethyl Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed and incubated with HRP conjugated Goat anti-human IgG1 Fc fragment (A80-104P, BETHYL) for IgG1 and Goat anti-human IgM antibody (A80-100P ...
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bioRxiv - Bioengineering 2024Quote: ... Sera containing approximately 100 µg of IgG (quantified using a Human IgG ELISA Quantification Set – Bethyl Laboratories) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cells were stained with Goat anti-human IgG conjugated with FITC (1:300 dilution; A80-119F, Bethyl) subsequently counterstained with DAPI (Sigma Aldrich Cat no D9542-10MG) ...
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bioRxiv - Bioengineering 2022Quote: Secretion of albumin was measured by Human Albumin Enzyme-Linked Immunosorbent Assay (ELISA) kit (Bethyl Laboratories, MA, USA). The ELISA assay was performed in clear flat-bottom 96-well microplates ...
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bioRxiv - Immunology 2022Quote: ... wells were washed 3x with PBS-T and a secondary antibody for that species was added (i.e., HRP goat-anti-human IgG, Rockland Immunochemicals, Pottstown, PA, USA; HRP goat-anti-dog IgG, Bethyl Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The following antibodies were used for immunostaining: Goat anti-human albumin antibody HRP Conjugated (A80-129P, Bethyl Laboratories, Inc.), goat anti-human IgG H&L (HRP ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 and were analyzed with the Bethyl’s human albumin ELISA quantification Set (Bethyl Laboratories, Inc., Cat# E80-129) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... Secondary labeling was performed with goat anti-human IgG-Fc horseradish peroxidase (HRP) (1:3000, Bethyl Labs, A80-104P). Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Secondary labeling was performed with goat anti-human IgG-Fc horseradish peroxidase (HRP) (1:3000, Bethyl Labs, A80-104P). Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Secondary labeling was performed with goat anti-human IgG-Fc horseradish peroxidase (HRP) (1:3000, Bethyl Labs, A80-104P). Antibody binding was detected with TMB/E HRP substrate (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and Ig kappa light chain concentration were quantified in the cell culture supernatant by ELISA according to the manunfacturer’s protocol (Human Kappa ELISA, Bethyl Laboratories).
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bioRxiv - Immunology 2022Quote: ... Bound Abs were detected with HRP-coupled polyclonal goat anti-human IgG Fc (#A80-104P)-specific Abs purchased from Bethyl Laboratories (Montgomery ...
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bioRxiv - Bioengineering 2020Quote: Quantification of antibody production in the supernatant of all our HEK293T cell cultures was done using an ELISA assay specific for human IgG (Bethyl Laboratories ...
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bioRxiv - Immunology 2021Quote: ... human sera from COVID-19 convalescent subjects was used as primary antibody followed by detection with goat anti-human IgG heavy and light chain HRP-conjugated (Bethyl). Precision Protein Streptactin HRP conjugate (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:10,000 dilution of goat anti-human IgG-Fc Antibody HRP (Bethyl, A80-104P) for 1hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... ELISA was performed following the manufacturer’s protocols (Human IgG ELISA E88-104 and Mouse IgG ELISA E99-131, Bethyl Laboratories). For adalimumab studies ...
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bioRxiv - Bioengineering 2022Quote: Albumin content in the supernatant media was evaluated using Human Albumin ELISA Quantitation Set (Bethyl Laboratories, catalogue no. E88-129). The concentration of urea in the medium was quantified calorimetrically using a urea assay kit (BioAssay Systems ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl blood was retro-orbitally collected each week and the hALB concentration was measured using a Human Albumin ELISA Quantitation Kit (Bethyl, TX) or a Latex agglutination turbidimetric immunoassay with a BioMajesty analyzer (JCA-BM6050 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then fixed with 4% PFA for 20 minutes at room temperature and labeled with the following antibodies: Anti-human albumin (A80-129A; Bethyl Laboratories) detected with a secondary antibody conjugated with Alexa-488 (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS-T and 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human or goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories) diluted in PBS-T with 4% milk powder was added and plates incubated for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Serum was separated by centrifugation and levels of human albumin were determined by an enzyme-linked immunosorbent assay (ELISA) using goat polyclonal capture and horseradish peroxidase– conjugated goat anti-human albumin detection antibodies (Bethyl Laboratories). Non- implanted FNRG mouse blood serum was included as a negative control.
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bioRxiv - Immunology 2021Quote: ... the plates were further incubated at room temperature for 1 hour with goat-anti human IgG-Fc fragment cross-adsorbed Ab (A80-340P; Bethyl Laboratories) at a 1 ...
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bioRxiv - Immunology 2023Quote: ... Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA, USA or Bethyl Laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... Liver Chip and Kidney Chip albumin concentrations were quantified using a human albumin ELISA quantitation kit (Bethyl Laboratories Inc., Montgomery, TX, USA) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... approximately 20 μl blood was collected each week from the tail and the hALB concentration was measured using a Human Albumin ELISA Quantitation Kit (Bethyl, Montgomery, TX). Livers were extracted at 8–10 weeks after transplantation and histologically analyzed.
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bioRxiv - Cell Biology 2022Quote: ... falciparum nucleosome assembly protein L (α-PfNAPL 1:500) (Singh et al., 2010) and goat antisera against human hemoglobin (α-hHb HRP 1:5000, Bethyl) in PBS with 2.5% skimmed milk and 0.05% Tween 20 for 2h at RT ...
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bioRxiv - Immunology 2022Quote: ... followed by a 30 min incubation with HRP-conjugated goat anti-human IgG Fc antibody at a 1:2500 dilution (Bethyl, A80-104A). Detection was completed using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 96-well plates were coated overnight with capture antibody (100 µg/mL; lactoferrin goat anti-human polyclonal, Bethyl Labs, Cat: A80-143A). The wells were washed and blocked using fish gelatin and incubated for 2 hours ...