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Citations for Bethyl :
701 - 742 of 742 citations for Mouse IgG1 Isotype Control Antibody 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS-T and 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human or goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories) diluted in PBS-T with 4% milk powder was added and plates incubated for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Serum was separated by centrifugation and levels of human albumin were determined by an enzyme-linked immunosorbent assay (ELISA) using goat polyclonal capture and horseradish peroxidase– conjugated goat anti-human albumin detection antibodies (Bethyl Laboratories). Non- implanted FNRG mouse blood serum was included as a negative control.
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bioRxiv - Cell Biology 2019Quote: ... cells were blocked for 60 minutes at 37°C in a heated humidified chamber and subsequently incubated with 53BP1 and Rad9 antibodies (Bethyl Laboratories) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: The following primary antibodies were used in this study: rabbit polyclonal antibodies against CDK5RAP2 (IHC00063: immunofluorescence [IF] 1:500, Western Blotting [WB] 1:1000; Bethyl Laboratories), Cep192 (A302–324A ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 5% non-fat dry milk and incubated over night at 4°C with the following primary antibodies: anti-CLUH (1:1000, cod: A301-764A, Bethyl Lab), anti-PGC-1α (1:1000 ...
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bioRxiv - Immunology 2021Quote: RBD antigen binding during selection rounds 3 and 5 was evaluated using a 1:1000 dilution of rabbit anti-His FITC secondary antibody (Bethyl Laboratories). RBD antigen binding during selection Round 4 was evaluated using a 1:1000 dilution of Streptavidin-Alexa Fluor 647 secondary (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2022Quote: ... 500 µg-1 mg of cell lysate was then incubated with 1:100 w/w myc tag antibody (Bethyl A190-105A) or anti-FLAG M2 (Millipore F1804 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed once for 20 min with 100 μL PBS and subsequently incubated for 1 hour at room temperature with either goat anti-ferret IgG-h+l horseradish peroxidase (HRP)-conjugated antibody (Cat#: A140-108P; Bethyl Laboratories), or goat anti-mouse HRP-conjugated antibody (Cat# ...
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bioRxiv - Molecular Biology 2022Quote: ... PML (two antibodies used; sheep anti-PML, Diagnostics Scotland, PML2A, 1:500; rabbit anti-PML, Bethyl Laboratories, A301-167A, 1:1000), SUMO (two antibodies used ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked with 5% non-fat dry milk in TBS supplemented 0.1% with Tween for one hour and incubated with the primary antibodies rabbit anti-U1C (Bethyl, A303-947A), rabbit anti-RBM39 (Sigma HPA0001591) ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed with PBS and SIV Env-bound antibodies were detected with a horseradish peroxidase (HRP)-conjugated goat anti-monkey IgG (H+L) (Bethyl Laboratory) and SureBlue TMB 1-Component Microwell Peroxidase Substrate (KPL) ...
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bioRxiv - Immunology 2023Quote: ... Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA, USA or Bethyl Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Triton 0.3% in PBS) for 1h RT and incubated overnight with primary antibodies against pRPA32 1:1000 (Bethyl, A300-246A-8), anti-human-mitochondria (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were then incubated with 50 μl Protein G Dynabeads conjugated with 20 μl of anti-EIF3B antibody (Bethyl A301-761A) for two hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated overnight at 4°C with the following primary antibodies: rabbit anti-L7 (Bethyl Laboratories, cat#A3100-741A-M, 1:1000), rabbit anti-L5 (kindly provided by Woolford lab ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin samples (25 μg per ChIP) were incubated overnight at 4°C with an antibody against FANCD2 (Bethyl, A302-174A, 2.5 μg). Rabbit IgG (Jackson ImmunoRes ...
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bioRxiv - Genomics 2022Quote: ... were washed with cold 1x PBS + 5 mg/mL BSA then combined with 8 µL antibody targeting ZEB1 (Bethyl, cat: A301-921A) or MEF2C (proteintech ...
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bioRxiv - Biochemistry 2021Quote: ... and probed with the following antibodies diluted in the Odyssey blocking buffer (Li-COR): anti-CFIm68 (Bethyl A301-358A-T, 1:1,000), anti-CFIm25 (Proteintech 10322-1-AP ...
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bioRxiv - Immunology 2022Quote: ... followed by a 30 min incubation with HRP-conjugated goat anti-human IgG Fc antibody at a 1:2500 dilution (Bethyl, A80-104A). Detection was completed using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C and run on a Western blot (as described in section 2.5.4) probed with BCLAF1 antibody (Bethyl A300-608A, 1:1000) and Asymmetric Di-Methyl Arginine ASYM25 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 96-well plates were coated overnight with capture antibody (100 µg/mL; lactoferrin goat anti-human polyclonal, Bethyl Labs, Cat: A80-143A). The wells were washed and blocked using fish gelatin and incubated for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin of ∼5×106 cells was incubated with 3ug antibody (RNAPII, rabbit polyclonal, Bethyl laboratories, A304-405A; PAF1, rabbit polyclonal, Bethyl laboratories, A300-172A) over night at 4°C ...
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bioRxiv - Physiology 2020Quote: ... The sections were fixed in 4% paraformaldehyde in PBS and incubated overnight at 4 °C with rabbit anti-NCOA4 antibody (1:100; Bethyl Laboratories. A302-272A) diluted in PBS with 2% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Finally there were subjected to immunofluorescence staining with ARID2 antibody (E-3, sc-166117 Santa Cruz, CA, USA or A302-230A, Bethyl Laboratories, USA), anti-phospho-Histone H2A.X Ser139 (γH2AX ...
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bioRxiv - Cell Biology 2020Quote: The details of the antibodies used for Immunoblotting (IB) and Immunofluorescence (IF) are given below PBRM1 (Bethyl Lab, # A301-591A, 1:1,000 IB), Lactate Dehydrogenase (LDH ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was blocked with 5% skim milk and incubated with primary antibodies: anti-Nanog (1:500, rabbit IgG, Bethyl/Fisher, A300-397A), anti-Gapdh (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... we utilized previously published methods to crosslink cell surface bound IgE using a goat anti-human IgE polyclonal antibody (αIgE; Bethyl Laboratories, Montgomery, TX) [34] ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated overnight with the following primary antibodies diluted in PBS with 0.1% Tween and 1% BSA: rabbit RPA2 pS4/8 (Bethyl, A300-245A, 1:200) and rabbit RPA2 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Membrane was blocked with 5% milk solution in 0.15% TBS-Tween 20 for 1 h and then incubated overnight with the following primary antibodies where they necessary: CRY1 (Bethyl Catalog No. A302-614A), CRY2 (Bethyl Catalog No ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer and incubated with diluted (1:200) anti-human IgG Fc protein antibody (FITC) (Bethyl Laboratories, CN: A80-104F) on ice for an additional 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... Then plates were washed 10 times with PBST and binding of VHHs to S1-Fc was detected with rabbit anti-6xHis Tag antibody HRP Conjugate (Bethyl Laboratories, Cat#A190-114P), diluted at 10 ng/mL in PBST and added at 100 µL/well ...
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bioRxiv - Biochemistry 2022Quote: ... 6 ug of appropriate antibody was added to the lysate (DNMT1: Abcam 19905 Lot No: GR3271225, Abcam 13537 Lot No: GR3184365, HNRNPH: Bethyl A300-511A, Lot: 2). Tubes were vortexed gently and then rotated at 4 °C for 2 hours ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with one of the following primary antibodies in Intercept Blocking Buffer supplemented with 0.1% (v/v) Tween-20 overnight at 4°C: an WB-optimized EMRE antibody (anti-EMRE; #A300-BL19208, 1:250; Bethyl Laboratories, Montgomery, TX, USA), anti-MICU1/CALC/CBARA1 (#LN2014887 ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 10μL antibody (anti-SAFB, Bethyl 812-300A; EZH2, Cell Signaling #5246) or 10μg IgG (Invitrogen #02-6502) ...
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bioRxiv - Developmental Biology 2023Quote: ... An anti-FRAS1 human antibody with 76% immunogen identity with zebrafish Fras1 including part of a highly conserved Calx-beta domain (Bethyl Labs, Cat #A305-622A) and anti-FREM2 human antibody with 77% immunogen identity with zebrafish Frem2a/b (Invitrogen PA5-55986 ...
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bioRxiv - Molecular Biology 2023Quote: ... were washed 3 times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 7.5μL antibody (anti-EZH2, Cell Signaling #5246; SAFB, Bethyl A300-812A; SUZ12, Cell Signaling #3737) or 3μL (7.5μg ...
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bioRxiv - Genetics 2024Quote: ... abnova H00055294-B01P) was used to pull down FBW7 and Bethy FBW7 antibodies (1:1000 in a 1:1 ratio, Bethyl A301-720A, and A301-721A) were used to detect FBW7 levels in controls and Fbxw7-null single cell clones ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and 100 ul of horseradish peroxidase (HRP)-conjugated goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories diluted in PBS, 0.1% BSA) was added for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories, diluted in PBS, 0.1% BSA) was added for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Standard curves were created using purified versions of each HIV inhibiting antibody or purified human IgG/kappa from normal serum (Bethyl, Montgomery, TX, USA, cat.: P80-111). Results were analyzed using GraphPad Prism v10 software to calculate a standard curve using a 4-parameter or 5-parameter sigmoidal algorithm ...