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Citations for Bethyl :
101 - 150 of 151 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and Ig kappa light chain concentration were quantified in the cell culture supernatant by ELISA according to the manunfacturer’s protocol (Human Kappa ELISA, Bethyl Laboratories).
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bioRxiv - Immunology 2022Quote: ... Bound Abs were detected with HRP-coupled polyclonal goat anti-human IgG Fc (#A80-104P)-specific Abs purchased from Bethyl Laboratories (Montgomery ...
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bioRxiv - Bioengineering 2020Quote: Quantification of antibody production in the supernatant of all our HEK293T cell cultures was done using an ELISA assay specific for human IgG (Bethyl Laboratories ...
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bioRxiv - Immunology 2021Quote: ... human sera from COVID-19 convalescent subjects was used as primary antibody followed by detection with goat anti-human IgG heavy and light chain HRP-conjugated (Bethyl). Precision Protein Streptactin HRP conjugate (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... the plates were incubated with a 1:10,000 dilution of goat anti-human IgG-Fc Antibody HRP (Bethyl, A80-104P) for 1hr at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Albumin content in the supernatant media was evaluated using Human Albumin ELISA Quantitation Set (Bethyl Laboratories, catalogue no. E88-129). The concentration of urea in the medium was quantified calorimetrically using a urea assay kit (BioAssay Systems ...
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bioRxiv - Immunology 2022Quote: ... ELISA was performed following the manufacturer’s protocols (Human IgG ELISA E88-104 and Mouse IgG ELISA E99-131, Bethyl Laboratories). For adalimumab studies ...
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bioRxiv - Immunology 2024Quote: ... IgM and IgA ELISAs were performed by coating 384-well Clear Flat Bottom Polystyrene High Bind Microplates with 10 µg/ml goat anti human IgG/IgM and IgA Fc (Bethyl) diluted in mQ water with 0,1 M Na2CO3/ NaHCO3 (Merck ...
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bioRxiv - Microbiology 2024Quote: ... for 1h at room temperature before incubation with 1:2,500-1:10,000-diluted horseradish peroxidase (HRP)-conjugated goat anti-human IgG-Fc antibody (Bethyl, A80-104A) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: Human tissue microarrays from 135 cases of pancreatic cancer were analyzed by immunohistochemistry staining using ADAR1 antibody (Bethyl Laboratories, Inc.). The study was approved by the Institutional Review Board at The University of Texas MD Anderson Cancer Center ...
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bioRxiv - Cancer Biology 2022Quote: Antibodies against the following proteins were used for western blotting: SAS10 (1:5000, BETHYL Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then fixed with 4% PFA for 20 minutes at room temperature and labeled with the following antibodies: Anti-human albumin (A80-129A; Bethyl Laboratories) detected with a secondary antibody conjugated with Alexa-488 (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS-T and 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human or goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories) diluted in PBS-T with 4% milk powder was added and plates incubated for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Serum was separated by centrifugation and levels of human albumin were determined by an enzyme-linked immunosorbent assay (ELISA) using goat polyclonal capture and horseradish peroxidase– conjugated goat anti-human albumin detection antibodies (Bethyl Laboratories). Non- implanted FNRG mouse blood serum was included as a negative control.
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bioRxiv - Immunology 2021Quote: ... the plates were further incubated at room temperature for 1 hour with goat-anti human IgG-Fc fragment cross-adsorbed Ab (A80-340P; Bethyl Laboratories) at a 1 ...
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bioRxiv - Immunology 2023Quote: ... Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA, USA or Bethyl Laboratories ...
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bioRxiv - Bioengineering 2024Quote: The supernatants were harvested at the end of differentiation and the amount of albumin secreted was measured using the human albumin ELISA kit (Bethyl laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... 96-well NUNC Maxisorp plates (ThermoScientific, 439454) were coated with 0.1 mL per well of goat anti-human IgG Fc fragment (Bethyl A80-104A) at 1:100 dilution in 0.05 M carbonate-bicarbonate (Sigma C30411 ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates from cell or organoid culture were extracted using RIPA lysis protocol (Bethyl Laboratories). Before use ...
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bioRxiv - Immunology 2021Quote: ... NTD or S2 SARS-CoV-2 protein diluted in carbonate-bicarbonate buffer (E107, Bethyl laboratories) were coated in 384-well plates (microplate clear ...
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bioRxiv - Cell Biology 2021Quote: ... 1mg of total heart proteins were immunoprecipitated with the following antibodies: anti-IDH2 (Bethyl Laboratories) or 3.5ug rabbit IgG (Bethyl Laboratories ...
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bioRxiv - Genetics 2020Quote: ... Zfp423 protein was detected using an antibody to residues 250-300 (A304-017A, Bethyl Labs) or residues near the carboxylterminal end of the protein (ABN410 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 96-well plates were coated overnight with capture antibody (100 µg/mL; lactoferrin goat anti-human polyclonal, Bethyl Labs, Cat: A80-143A). The wells were washed and blocked using fish gelatin and incubated for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... followed by a 30 min incubation with HRP-conjugated goat anti-human IgG Fc antibody at a 1:2500 dilution (Bethyl, A80-104A). Detection was completed using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated at room temperature for 1 hour with goat anti-human IgG-Fc fragment cross adsorbed Ab (Bethyl Laboratories, A80-340P) at 1:10,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated at room temperature for 1 hour with goat anti-human IgG-Fc fragment cross adsorbed Ab (Bethyl Laboratories, A80-340P) at 1:10,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated at room temperature for 1 hour with goat anti-human IgG-Fc fragment cross adsorbed Ab (Bethyl Laboratories, A80-340P) at 1:10,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The plates with huACE2-IgHu were then incubated at room temperature for 1 hour with goat anti-human IgG-Fc fragment cross adsorbed Ab (Bethyl Laboratories, A80-340P) at 1:10,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... The plates were further incubated at room temperature for 1 hour with goat anti-human IgG-Fc fragment cross adsorbed Ab (Bethyl Laboratories, A80-340P) at 1:10,000 dilution ...
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bioRxiv - Immunology 2024Quote: ... we utilized previously published methods to crosslink cell surface bound IgE using a goat anti-human IgE polyclonal antibody (αIgE; Bethyl Laboratories, Montgomery, TX) [34] ...
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bioRxiv - Bioengineering 2024Quote: ... The residual amount of laminin in FLGs was estimated by the following equation: Albumin concentrations in supernatants were measured using a human albumin ELISA quantification kit (Bethyl Lab, #E80-129). Sample absorbance was measured with a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Microbiology 2024Quote: IgA levels in human breast milk whey were quantified using a commercial ELISA kit following the manufacturer’s recommendations (Bethyl Laboratories, Montgomery, Texas, USA). Whey samples were assayed in duplicate at a dilution of 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... we detected our protein of interest using the following antibodies: α-INTS1 (Bethyl Laboratories, #A300-361A), α-INTS3 (Sigma Prestige ...
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bioRxiv - Developmental Biology 2023Quote: ... An anti-FRAS1 human antibody with 76% immunogen identity with zebrafish Fras1 including part of a highly conserved Calx-beta domain (Bethyl Labs, Cat #A305-622A) and anti-FREM2 human antibody with 77% immunogen identity with zebrafish Frem2a/b (Invitrogen PA5-55986 ...
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bioRxiv - Neuroscience 2022Quote: Custom made antibodies aging the killifish DDX5 protein (XP_015809881.1) were generated in rabbits and performed by Bethyl, using their standard protocol that peptide synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used for IP: anti-SAS10 (1:100, BETHYL Laboratories, A302-067A), anti-TRIM29 (1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of eluted proteins was made by western blot assay using anti-FUS (A300-294A, Bethyl Laboratories) to identify FUS protein and anti-His (HIS.H8 ...
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bioRxiv - Cell Biology 2022Quote: ... the proteins were labelled by anti-Nup188 (Catalog No. A302-322A, Bethyl Laboratories, Montgomery, TX, 1:5000) and anti-γ-Tubulin (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Standard curves were created using purified versions of each HIV inhibiting antibody or purified human IgG/kappa from normal serum (Bethyl, Montgomery, TX, USA, cat.: P80-111). Results were analyzed using GraphPad Prism v10 software to calculate a standard curve using a 4-parameter or 5-parameter sigmoidal algorithm ...
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bioRxiv - Genetics 2020Quote: ... proteins were transferred unto a PVDF membrane which was probed using antibodies for TMEM127 (1:10,000, rabbit, Bethyl Labs), GFP (1:2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane and detected with antibodies against Rtf1 (Bethyl Labs A301-329A, 1:4000) and beta-actin (Sigma A1978 ...
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bioRxiv - Biophysics 2022Quote: ... the amount of CcdB protein expressed on the yeast cell surface was estimated by chicken anti-HA antibodies from Bethyl labs (1˸600 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies for proteins of interest were diluted to 1:200 (ER 6F11) or 1:2500 (Bethyl MDC1 A300-051A). DNA was stained for imaging using Duolink mounting medium with DAPI (Sigma #DUO82040) ...
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bioRxiv - Microbiology 2021Quote: ... and 2 mg of total protein was incubated with 15 µl Goat anti-Myc Antibody Tag Agarose Immobilized (Bethyl, Montgomery, TX) at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The IP was then performed as above using 2-10 mg of protein and 1 μg of rabbit anti-Sec24C (A304-760A, Bethyl Laboratories) per 1 mg of protein or 0.9 μg of rabbit anti-Sec24D (14687 ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were then incubated with 50 μl Protein G Dynabeads conjugated with 20 μl of anti-EIF3B antibody (Bethyl A301-761A) for two hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and an equal mass of WT and KO nuclear lysate (∼30 μg protein) was immunoprecipitated by 1 μg WDR5 (Bethyl, A302-429A) or normal rabbit IgG (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... 4.5-5 mg total protein were used for each replicate with 4 ug of KIF1C antibody (Bethyl Kif1c/LTXS1 antibody, A301-072A). For western blots ...
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bioRxiv - Molecular Biology 2024Quote: ... The precleared lysates were incubated with 20 µL of protein A/G plus agarose beads and 2 µg of BCCIP antibody (Bethyl; A302-196A). Beads were washed three times in cold MCLB buffer and resuspended in 30 µL of MCLB buffer ...
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bioRxiv - Genomics 2024Quote: ... The chromatin immunoprecipitation was carried out by overnight incubation of pre-cleared chromatin with Dynabeads Protein A coated with 10 μg of SMC1 (Bethyl Laboratories, A300-055A) or 10 μg CTCF (Abclonal ...