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• Cancer mutations rewire the RNA methylation specificity of METTL3-METTL14

  Chi Zhang, et al., bioRxiv - Biochemistry 2023

  Quote: ... 0.05% Tween 20) for 1 hour and incubated with primary antibodies (anti-METTL3, Bethyl A301-567A, 1:1,000 (v/v); anti-METTL14 ...
• Modular Tissue-in-a-CUBE Platform to Model Blood-brain-Barrier (BBB) and Brain Interaction

  Isabel Koh, Masaya Hagiwara, bioRxiv - Bioengineering 2023

  Quote: ... followed by IF+G with 1% goat anti-mouse IgG (Bethyl Laboratories, A90-116A) for 20 min ...
• Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1

  Stephen Abini-Agbomson, et al., bioRxiv - Biochemistry 2023

  Quote: ... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...
• RNA transcripts suppress G-quadruplex structures through G-loop formation

  Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer, bioRxiv - Biochemistry 2023

  Quote: ... xlXPF26 and hsRNA Polymerase II subunit A (Bethyl) were previously described ...
• Mechanistic insights into the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control

  Ryosuke Ishimura, et al., bioRxiv - Biochemistry 2023

  Quote: ... UFL1 (A303-456A; Bethyl Laboratories ...
• The RPA complex orchestrates K63-linked deubiquitination via ZUP1

  Benjamin Foster, et al., bioRxiv - Biochemistry 2023

  Quote: ... rabbit polyclonal RPA32 (Bethyl, A300-244A), rabbit polyclonal RPA70 (Cell Signaling Technologies ...
• Comparative proximity biotinylation implicates RAB18 in sterol mobilization and biosynthesis

  Robert S. Kiss, et al., bioRxiv - Cell Biology 2023

  Quote: ... An antibody to RAB3GAP1 was obtained from Bethyl Labs (Montgomery ...
• Template-assisted covalent modification of DCAF16 underlies activity of BRD4 molecular glue degraders

  Yen-Der Li, et al., bioRxiv - Biochemistry 2023

  Quote: The following antibodies were used: anti-BRD4 (Bethyl Laboratories, A301-985A100), anti-β-actin (Cell Signaling Technology ...
• Identification and evaluation of small-molecule inhibitors against the dNTPase SAMHD1 via a comprehensive screening funnel

  Si Min Zhang, et al., bioRxiv - Biochemistry 2023

  Quote: ... or from Bethyl Laboratories Inc ...
• The Loss of the E3 ubiquitin ligase TRIP12 inhibits Pancreatic Acinar Cell Plasticity and Tumor Cell Metastatic Capacity

  Manon Brunet, et al., bioRxiv - Cancer Biology 2023

  Quote: ... They were immunostained with anti-TRIP12 (1:1000, Bethyl Laboratories A301) using standard procedures.
• The Loss of the E3 ubiquitin ligase TRIP12 inhibits Pancreatic Acinar Cell Plasticity and Tumor Cell Metastatic Capacity

  Manon Brunet, et al., bioRxiv - Cancer Biology 2023

  Quote: ... They were immunostained with an anti-TRIP12 (1:1000, Bethyl Laboratories #A301) using standard procedures.
• High prevalence and dependence of centrosome clustering in mesenchymal tumors and leukemia

  N. Moreno-Marin, et al., bioRxiv - Cancer Biology 2023

  Quote: ... After incubation with horseradish peroxidase–conjugated secondary antibodies (1:5000; Bethyl Laboratories), the immunolabeled proteins were detected by WesternBright™ ECL Luminol Reagent (Advansta ...
• NF-κB/p52 augments ETS1 binding genome-wide to promote glioma progression

  Nicholas Sim, Li Yinghui, bioRxiv - Cancer Biology 2023

  Quote: ... and non-specific rabbit IgG (Bethyl Laboratories; P120-101). Eluted DNA was used for qPCR or sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (NEB ...
• Human papillomavirus 16 E2 interaction with TopBP1 is required for E2 and viral genome stability during the viral life cycle

  Apurva T. Prabhakar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... TopBP1 1:1,000 (Bethyl, catalog no. A300-111A), GAPDH 1:10,000 (Santa Cruz ...
• NF-κB/p52 augments ETS1 binding genome-wide to promote glioma progression

  Nicholas Sim, Li Yinghui, bioRxiv - Cancer Biology 2023

  Quote: ... using the following antibodies: anti-NFkB2 (Bethyl Laboratories; A300-BL7039), anti-ETS1 (Active Motif ...
• Signaling protein abundance modulates the strength of the Spindle Assembly Checkpoint

  Chu Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-BubR1 (A300-386A, Bethyl Laboratories), and anti-Mad1 (ab184560 ...
• Loss of the endocytic tumor suppressor HD-PTP phenocopies LKB1 and promotes RAS-driven oncogenesis

  Chang-Soo Seong, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Membranes were blocked in 5% BSA or 5% milk for one hour at room temperature and then incubated overnight at 4°C with rabbit anti-HD-PTP (1:1,000, Bethyl Laboratories), mouse anti-Integrinβ1/CD29(1:1,000 BD Biosciences) ...
• Notch1 cortical signaling regulates epithelial architecture and cell-cell adhesion

  Matthew J. White, et al., bioRxiv - Cell Biology 2023

  Quote: ... FAM83H antibody (1:1000 WB) was from Bethyl Laboratories ...
• Aurora B Kinase Dependent Phosphorylation of Keratin 8 is required for Cytokinesis in Mammalian Cells of Epithelial Origin

  Busra Harmanda, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit polyclonal INCENP (Bethyl Laboratories ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Primary antibodies were prepared in blocking solution as following dilutions: 53BP1 (1:500, IHC-00001; Bethyl Laboratories), 53BP1 (1:300 ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BLM (1:250, Cat# A300-110A; Bethyl Laboratories), FLAG (1:200 ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... SMC5 (1:400, A300-236A; Bethyl Laboratories), SUMO-2/3 (1:250 ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... KDM2A (1:1,000, A301-476A; Bethyl Laboratories), SENP6 (1:500 ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... SMC5 (1:2000, A300-236A; Bethyl Laboratories), and TUBULIN (1:2,000 ...
• Histone demethylase KDM2A is a selective vulnerability of cancers relying on alternative telomere maintenance

  Fei Li, et al., bioRxiv - Cancer Biology 2023

  Quote: ... γH2AX (1:2,000, A300-081A; Bethyl Laboratories), phospho-Histone H3 (Ser10 ...
• Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

  Yi-Chang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-γH2AX (1:3000; Cat# A300-081, Bethyl), anti-histone H3 (1:20000 ...
• Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

  Yi-Chang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-SMARCA3 (HLTF, 1:3000, Cat# A300-230A, Bethyl laboratories), anti-Rad6 (1:3000 ...
• Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination

  Yi-Chang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-phospho-RPA32 (Ser33) (1:3000; Cat# A300-246A, Bethyl Laboratories), anti-trimethyl-histone H3 (Lys4 ...
• MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch

  Simon T. Jakobsen, et al., bioRxiv - Genomics 2023

  Quote: ... BRD4 (Bethyl Laboratories, A301-985A100).
• MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch

  Simon T. Jakobsen, et al., bioRxiv - Genomics 2023

  Quote: ... Primary antibodies: KDM3A (Bethyl Laboratories, A301-538A, 1:1000), MYC (Cell Signaling ...
• MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch

  Simon T. Jakobsen, et al., bioRxiv - Genomics 2023

  Quote: qPLEX-RIME was performed as described previously (Papachristou et al., 2018) with five biological replicates using MED1 (Bethyl Laboratories, A300-793A) or GR (Cell Signaling ...
• Recovery of protein synthesis to measure transcription-coupled DNA repair in living cells and tissues

  Melanie van der Woude, et al., bioRxiv - Genetics 2023

  Quote: ... CSB (Bethyl), XPF (Santa Cruz) ...
• ADAR1p150 Prevents MDA5 and PKR Activation via Distinct Mechanisms to Avert Fatal Autoinflammation

  Shi-Bin Hu, et al., bioRxiv - Genetics 2023

  Quote: ... ILF2 (Bethyl laboratories, A303-147A), GAPDH (Santa Cruz Biotechnology ...
• Recovery of protein synthesis to measure transcription-coupled DNA repair in living cells and tissues

  Melanie van der Woude, et al., bioRxiv - Genetics 2023

  Quote: ... XPC (Bethyl), Tubulin (Sigma Aldrich ...
• A cytokine-responsive promoter is required for distal enhancer function mediating the hundreds-fold increase in milk protein gene expression during lactation

  Hye Kyung Lee, Chengyu Liu, Lothar Hennighausen, bioRxiv - Genomics 2023

  Quote: ... MED1 (Bethyl Laboratory, A300-793A), H3K27ac (Abcam ...
• Gut-associated lymphoid tissue attrition associates with response to anti-α4β7 therapy in ulcerative colitis

  Pablo Canales-Herrerias, et al., bioRxiv - Immunology 2023

  Quote: ELISA was performed using Mouse IgA ELISA Quantitation Set or Mouse IgG ELISA Quantitation Set (Bethyl Laboratories) according to the manufacturer’s protocol with minor modifications ...
• Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma

  Zoltán Kellermayer, et al., bioRxiv - Immunology 2023

  Quote: ... as capture antibody and an HRP-labeled goat anti-mouse IgG2b (A90-109P, Bethyl Laboratories, Inc) as detection antibody (both previously sold as part of the Mouse IgG2b ELISA Quantitation Set ...
• Immune-mediated tumor control in the 5TGM1 transfer model of multiple myeloma

  Zoltán Kellermayer, et al., bioRxiv - Immunology 2023

  Quote: ... ELISA was performed using a purified goat anti-mouse IgG2b (A90-109A, Bethyl Laboratories, Inc) as capture antibody and an HRP-labeled goat anti-mouse IgG2b (A90-109P ...
• PerTurboID: A targeted in situ method to measure changes in a local protein environment reveals the impact of kinase deletion on cytoadhesion in malaria causing parasites

  Heledd Davies, et al., bioRxiv - Cell Biology 2023

  Quote: ... Anti-biotin antibodies were added (equal mix of Bethyl laboratories ...
• Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeast

  Yong-jie Xu, et al., bioRxiv - Genetics 2023

  Quote: ... The custom antibody against phosphorylated Chk1-Ser345 used in this study was generated in rabbits and purified by Bethyl Laboratories (Montgomery ...
• CCDC15 localizes to the centriole inner scaffold and regulates centriole integrity and ciliogenesis

  Melis D. Arslanhan, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti CEP152 (1:1000, Bethyl, A302-479), mouse anti α-tubulin (DM1A ...
• Specialized actin nanoscale layers control focal adhesion turnover

  Reena Kumari, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-KANK1 (Bethyl laboratories, A301-882A), rabbit anti-KANK2 (Sigma-Aldrich ...
• Constitutive protein degradation induces acute cell death via proteolysis products

  Si-Han Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... BRD4 C-term (A700-004, Bethyl), BRD2 (ab139690 ...
• Constitutive protein degradation induces acute cell death via proteolysis products

  Si-Han Chen, et al., bioRxiv - Cell Biology 2023

  Quote: ... BRD3 (A302-368A, Bethyl), Caspase-3 (9662 ...
• Immunostimulatory effects of Bacillus coagulans SANK70258

  Yuki Ikeda, et al., bioRxiv - Immunology 2023

  Quote: ... or mouse IgA ELISA Quantification Set (#E90-103, Bethyl Laboratories).
• Phosphorylation Status Of MUS81 Is A Modifier Of Olaparib Sensitivity In BRCA2-Deficient Cells

  Francesca Blandino, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were incubated with anti-BRCA2 antibody (rabbit-polyclonal, Bethyl, 1:200) for 1h at 37°C in a humidified chamber ...
• Loss of genome maintenance accelerates podocyte damage and aging

  Fabian Braun, et al., bioRxiv - Cell Biology 2023

  Quote: Urinary albumin levels were measured with a mouse albumin ELISA kit (mouse albumin ELISA kit; Bethyl Labs, Montgomery, TX, USA). Urinary creatinine kit (Cayman Chemical ...
• The nucleolar protein GNL3 prevents resection of stalled replication forks

  Rana Lebdy, et al., bioRxiv - Molecular Biology 2023

  Quote: ... incubated overnight at 4°C with primary antibodies directed against ORC2 (A302-734A, Bethyl Laboratories) and GNL3 (sc-166460 ...
• The nucleolar protein GNL3 prevents resection of stalled replication forks

  Rana Lebdy, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Ser4/8 Phospho-RPA32 (Bethyl A300-245A), RPA32 (Calbiochem NA18) ...
• The nucleolar protein GNL3 prevents resection of stalled replication forks

  Rana Lebdy, et al., bioRxiv - Molecular Biology 2023

  Quote: ... NOP1 (Novus NBP2-46881) and GNL3 (Bethyl A300-600A).