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Citations for Bethyl :
601 - 650 of 666 citations for Histone Lysine N Methyltransferase EZH2 EZH2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 2h at room temperature in a goat anti-rabbit secondary Cy3 antibody (1:250, A120-201C3, Bethyl), and/or goat anti-chicken Cy5 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... then added 100 μl per well of horseradish peroxidase (HRP) conjugated detection antibodies against either horse IgG1 (A70-124P 16; Bethyl Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... These fragments incubated in ChIP dilution buffer with an αSMC3 antibody (5 μg/sample; Bethyl, Montgomery, Texas, USA, #A300-060A). Simultaneously ...
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bioRxiv - Cell Biology 2024Quote: ... One per cent of the sample was used as the input control and the remaining extracts were incubated with 10 μg DLST antibody (Bethyl) or IgG at 41°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... the endogenous MED1 was immunoprecipitated from whole cell lysate of HEK293T by anti-MED1 antibody (Bethyl, A300-793A, 1:800) followed with protein A sepharose CL-4B beads (Amersham ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 200–500 μg of nuclear lysates were incubated with 2 μg of SMARCD1 antibody (Bethyl A301-594A) and 50 μg of Dynabeads Protein G (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... antibodies and dilutions were as follows: Goat anti-Mouse IgG-h+1 HRP conjugated (1:5000, Bethyl Labs A90-116P), Rabbit TruBlot anti-Rabbit IgG (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were probed with the following antibodies overnight at 4°C under stirring: anti-hDICER (1:1000, A301-937A, Euromedex, Bethyl), anti-PKR (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Digested lysates were briefly sonicated using a 10s/50s on/off cycle for three times at 40% power and clarified by centrifugation at 20,000g for 10 min at 4°C and supernatants were incubated with 10 μg FUS antibody (Bethyl, A300-302A) overnight at 4°C with rotation ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was incubated with anti-bovine β-lactoglobulin horse radish peroxidase (HRP)-conjugated antibody (Bethyl Lab A10-125P 1:100K) for 3 hours and immune-reactivity determined using chemiluminescence ...
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bioRxiv - Cancer Biology 2019Quote: ... The second direction is standard SDS 5-10% PAGE followed by staining with Coomassie Brilliant Blue G-250 (#31-4-58-1, Helicon, Russia) or ELISA using anti-NCL antibodies (#a300-711A, Bethyl, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Blots were blocked with 5% milk/TBS+0.1% Tween-20 (TBST) and incubated with primary antibodies against Brf1 (Bethyl a301-228a), p53 (Cell Signaling 2524 ...
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bioRxiv - Microbiology 2021Quote: ... and 2 mg of total protein was incubated with 15 µl Goat anti-Myc Antibody Tag Agarose Immobilized (Bethyl, Montgomery, TX) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... PAF1 and STAT2 KO were verified by Sanger sequencing and immunoblotting using antibodies against PAF1 (1:1000, Bethyl Labs, A300-173A) or STAT2 (1:200 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then fixed with 4% PFA for 20 minutes at room temperature and labeled with the following antibodies: Anti-human albumin (A80-129A; Bethyl Laboratories) detected with a secondary antibody conjugated with Alexa-488 (Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Slices were incubated overnight at 4°C with the following primary antibodies: sheep anti-rat albumin (1:100; Bethyl lab Inc.) mouse anti-multidrug resistance associated protein (MRP)-2 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... 100-500 ug lysate was incubated with either Mouse anti-Menin monoclonal antibody (Bethyl Laboratories, #A500-003A, 1:100 v/v) or Rat anti-GFAP monoclonal antibody (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS-T and 100 μl of horseradish peroxidase (HRP)-conjugated goat anti-human or goat anti-pig Fc fragment secondary antibody (Bethyl Laboratories) diluted in PBS-T with 4% milk powder was added and plates incubated for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Serum was separated by centrifugation and levels of human albumin were determined by an enzyme-linked immunosorbent assay (ELISA) using goat polyclonal capture and horseradish peroxidase– conjugated goat anti-human albumin detection antibodies (Bethyl Laboratories). Non- implanted FNRG mouse blood serum was included as a negative control.
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bioRxiv - Cell Biology 2019Quote: ... cells were blocked for 60 minutes at 37°C in a heated humidified chamber and subsequently incubated with 53BP1 and Rad9 antibodies (Bethyl Laboratories) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Corresponding secondary horseradish peroxidase-conjugated antibodies were used: anti-mouse and anti-rabbit (Jackson Immuno Research Laboratories) and anti-rat (A110-105P, Bethyl Laboratories). Membranes were incubated with a lab-made enhanced chemiluminescence reagent containing 100 mM Tris (pH 8.5) ...
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bioRxiv - Cell Biology 2020Quote: The following primary antibodies were used in this study: rabbit polyclonal antibodies against CDK5RAP2 (IHC00063: immunofluorescence [IF] 1:500, Western Blotting [WB] 1:1000; Bethyl Laboratories), Cep192 (A302–324A ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 5% non-fat dry milk and incubated over night at 4°C with the following primary antibodies: anti-CLUH (1:1000, cod: A301-764A, Bethyl Lab), anti-PGC-1α (1:1000 ...
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bioRxiv - Immunology 2021Quote: RBD antigen binding during selection rounds 3 and 5 was evaluated using a 1:1000 dilution of rabbit anti-His FITC secondary antibody (Bethyl Laboratories). RBD antigen binding during selection Round 4 was evaluated using a 1:1000 dilution of Streptavidin-Alexa Fluor 647 secondary (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2022Quote: ... 500 µg-1 mg of cell lysate was then incubated with 1:100 w/w myc tag antibody (Bethyl A190-105A) or anti-FLAG M2 (Millipore F1804 ...
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bioRxiv - Molecular Biology 2022Quote: ... adult male mouse livers from Zhx2WT and Zhx2KO mice were harvested and processed for ChIP with rabbit anti-Zhx2 antibodies (Bethyl labs) or control IgG using the Magna ChIP HiSense kit (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed once for 20 min with 100 μL PBS and subsequently incubated for 1 hour at room temperature with either goat anti-ferret IgG-h+l horseradish peroxidase (HRP)-conjugated antibody (Cat#: A140-108P; Bethyl Laboratories), or goat anti-mouse HRP-conjugated antibody (Cat# ...
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bioRxiv - Molecular Biology 2022Quote: ... PML (two antibodies used; sheep anti-PML, Diagnostics Scotland, PML2A, 1:500; rabbit anti-PML, Bethyl Laboratories, A301-167A, 1:1000), SUMO (two antibodies used ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked with 5% non-fat dry milk in TBS supplemented 0.1% with Tween for one hour and incubated with the primary antibodies rabbit anti-U1C (Bethyl, A303-947A), rabbit anti-RBM39 (Sigma HPA0001591) ...
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bioRxiv - Immunology 2023Quote: ... as detection antibody (both previously sold as part of the Mouse IgG2b ELISA Quantitation Set, Cat# E90-109, Bethyl Laboratories, Inc), and mouse IgG2b Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed with PBS and SIV Env-bound antibodies were detected with a horseradish peroxidase (HRP)-conjugated goat anti-monkey IgG (H+L) (Bethyl Laboratory) and SureBlue TMB 1-Component Microwell Peroxidase Substrate (KPL) ...
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bioRxiv - Immunology 2023Quote: Airway serum albumin was quantified by ELISA using BALF samples and anti-mouse albumin coating- and HRP-conjugated anti-mouse albumin detection antibodies (both Bethyl Laboratories) and a purified mouse serum albumin standard (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Microarrays were probed with sera and antibody binding detected by incubation with fluorochrome-conjugated goat anti-human IgG (Jackson ImmunoResearch, West Grove, PA, USA or Bethyl Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... 3% BSA in PBS solution for 30min and then incubated with goat anti-mouse albumin antibody (A90-134A-17; Bethyl Laboratories) at 1:200 in blocking buffer overnight at 4°C in a humidity chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... Triton 0.3% in PBS) for 1h RT and incubated overnight with primary antibodies against pRPA32 1:1000 (Bethyl, A300-246A-8), anti-human-mitochondria (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were then incubated with 50 μl Protein G Dynabeads conjugated with 20 μl of anti-EIF3B antibody (Bethyl A301-761A) for two hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... then incubated overnight at 4°C with the following primary antibodies: rabbit anti-L7 (Bethyl Laboratories, cat#A3100-741A-M, 1:1000), rabbit anti-L5 (kindly provided by Woolford lab ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin samples (25 μg per ChIP) were incubated overnight at 4°C with an antibody against FANCD2 (Bethyl, A302-174A, 2.5 μg). Rabbit IgG (Jackson ImmunoRes ...
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bioRxiv - Genomics 2022Quote: ... were washed with cold 1x PBS + 5 mg/mL BSA then combined with 8 µL antibody targeting ZEB1 (Bethyl, cat: A301-921A) or MEF2C (proteintech ...
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bioRxiv - Immunology 2019Quote: ... were coated overnight at 4°C with 100 µL/well of 10 µg/mL goat anti-mouse IgA antibody (Bethyl Laboratories, Inc.) for total IgA ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... and probed with the following antibodies diluted in the Odyssey blocking buffer (Li-COR): anti-CFIm68 (Bethyl A301-358A-T, 1:1,000), anti-CFIm25 (Proteintech 10322-1-AP ...
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bioRxiv - Immunology 2022Quote: ... followed by a 30 min incubation with HRP-conjugated goat anti-human IgG Fc antibody at a 1:2500 dilution (Bethyl, A80-104A). Detection was completed using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C and run on a Western blot (as described in section 2.5.4) probed with BCLAF1 antibody (Bethyl A300-608A, 1:1000) and Asymmetric Di-Methyl Arginine ASYM25 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Blots were rinsed with TBS-T three times for 5 minutes each and stained with secondary HRP antibody (Bethyl A90-516P (mouse), A120-201P (rabbit ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 96-well plates were coated overnight with capture antibody (100 µg/mL; lactoferrin goat anti-human polyclonal, Bethyl Labs, Cat: A80-143A). The wells were washed and blocked using fish gelatin and incubated for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin of ∼5×106 cells was incubated with 3ug antibody (RNAPII, rabbit polyclonal, Bethyl laboratories, A304-405A; PAF1, rabbit polyclonal, Bethyl laboratories, A300-172A) over night at 4°C ...
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bioRxiv - Physiology 2020Quote: ... The sections were fixed in 4% paraformaldehyde in PBS and incubated overnight at 4 °C with rabbit anti-NCOA4 antibody (1:100; Bethyl Laboratories. A302-272A) diluted in PBS with 2% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Finally there were subjected to immunofluorescence staining with ARID2 antibody (E-3, sc-166117 Santa Cruz, CA, USA or A302-230A, Bethyl Laboratories, USA), anti-phospho-Histone H2A.X Ser139 (γH2AX ...