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Citations for Addgene :
601 - 650 of 976 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... template libraries were constructed by PCR sewing and cloned into pRSII413 (a gift from Steven Haase, Addgene plasmid #35450 ...
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bioRxiv - Microbiology 2023Quote: ... the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed ...
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bioRxiv - Cell Biology 2023Quote: ... the AGO2 CDS was PCR-amplified using pFRT/FLAG/HA-DEST EIF2C2 plasmid (Addgene: Cat. # 19888[57]) as template and Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... we generated PCR amplified EF1a promoter and TetR-NLS signal cassette from pLV-tTRKRAB-red (Addgene, #12250). 3xFlag-KRAB was PCR amplified from the same vector with a forward primer including the sequence encoding 3xFlag and SV40pA amplified from lentiCRIPSRv2 ...
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bioRxiv - Genetics 2023Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
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bioRxiv - Genetics 2023Quote: To replace nvYFP with mCherry we PCR amplified mCherry from pQC NLS mCherry IX (Addgene plasmid 37354) using primers #1833 and #1834 and PCR amplified attB-Dfd-3XP3 from pAttB-Dfd-3XP3-nvYfp with primers #1831 and #1832 and performed Gibson assembly to generate plasmid #795 = pAttB-Dfd-3xP3-mCherry.
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bioRxiv - Developmental Biology 2023Quote: ... we added the T7 promoter sequence to the sgRNA template by PCR amplification of px330 (Addgene, 42230) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... human RXRa cDNA was PCR-amplified from pSV-Sport-RXRα (a gift from Bruce Spiegelman; Addgene #8882)68 ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR cassette was transfected into HeLa cells with a helper plasmid containing AsCas12a endonuclease (Addgene 89353). Following cleavage ...
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bioRxiv - Developmental Biology 2023Quote: PCR amplification of the repair template was performed using 50 ng of plasmid containing mNeonGreen (Addgene 125134) as template in a touchdown PCR reaction (annealing temperature decreased 1°C from 65°C to 50°C for the first 15 cycles followed by 20 cycles with an annealing temperature of 50°C ...
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bioRxiv - Genetics 2024Quote: ... the T7 promoter sequence was added to the sgRNA template by PCR amplification using pX335 (Addgene #42335) with Phusion high-fidelity DNA Polymerase Kit (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA sequences between nucleotides 1-3877 and 4236-4617 were PCR amplified from V5-GFP-P180 (Addgene #92150) and the two fragments were assembled and cloned into pEGFP(A206K)-N1 between XhoI and BamHI sites by GIBSON assembly ...
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bioRxiv - Genetics 2021Quote: TPM2 mammalian expression constructs were generated by PCR subcloning variants from pUASt.attB into pCMV-IRES-eGFP (Addgene 78264) using XbaI/EcoR1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then cloned into the pPD95.77 vector (Fire Lab C. elegans vector kit; Addgene, Cambridge, MA) via the SalI and BamHI sites ...
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bioRxiv - Neuroscience 2021Quote: hM4D(Gi) was amplified by PCR from pcDNA5/FRT-HA-hM4D(Gi) (Addgene #45548 (Armbruster et al., 2007)) using forward primer ...
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bioRxiv - Neuroscience 2021Quote: Kir was amplified by PCR from pCAG-Myc-Kir2.1E224G/Y242F-2A-EGFP (similar to pCAG-Kir2.1-T2A-tdTomato, Addgene #60598 ...
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bioRxiv - Genetics 2020Quote: ... A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234; http://n2t.net/addgene:54234 ; RRID:Addgene_54234), while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213925 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were then subcloned into the pDEST-ORF-v1 (a kind gift from Darren Saunders, Addgene, #73637) (Croucher et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs, Addgene # 25999) (Beronja et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ; http://n2t.net/addgene:122848; RRID:Addgene_122848) (94 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA sequences of full-length CAD and its fragments were amplified by PCR from pcDNA3.1-HisFlag-CAD (Addgene) and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... the FRT-stop-FRT cassette (
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bioRxiv - Neuroscience 2020Quote: ... mScarlet-i cDNA was amplified by PCR from a pmScarlet-i_C1 plasmid (gift from Dorus Gadella, Addgene #85044) (Bindels et al. ...
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bioRxiv - Genetics 2022Quote: ... we transformed BY with a DNA fragment created by PCR amplifying the NatMX cassette from plasmid from Addgene plasmid #35121 (a gift from John McCusker ...
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bioRxiv - Microbiology 2022Quote: The Ca2+ FRET reporter Twitch-2B was PCR amplified from the plasmid Twitch-2B pRSETB obtained from Addgene [23] using the primers T2B1 and T2B2 (Table 1 ...
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bioRxiv - Immunology 2022Quote: ... The MSCV-N EBNA3A plasmid used as a backbone for PCR was a gift from Karl Munger (Addgene plasmid # 37956 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned into pICSL01005 (Level 0 acceptor for CDS no stop modules, Addgene no.47996) as a level 0 module for Golden Gate assembly ...
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bioRxiv - Neuroscience 2021Quote: Tetanus toxin light chain (TeTxLC) was amplified by PCR from pGEMTEZ-TeTxLC (Addgene #32640, (Yu et al., 2004)) using forward primer ...
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bioRxiv - Neuroscience 2021Quote: ... hM3Dq-mCherry cDNA was polymerase chain reaction (PCR)-amplified from pAAV-hSyn-hM3D(Gq)-mCherry (Addgene plasmid #50474) and transferred to the lentiviral vector CSIV with the CAG promoter ...
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bioRxiv - Immunology 2022Quote: ... a Cas9-p2a-EGFP construct was PCR amplified from the Lenti-Cas9-EGFP plasmid obtained from Addgene (63592). Then ...
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bioRxiv - Immunology 2022Quote: ... The MSCV-N EBNA3A plasmid used as a backbone for PCR was a gift from Karl Munger (Addgene plasmid # 37956; http://n2t.net/addgene:37956.; RRID:Addgene_37956) (Rozenblatt-Rosen et al. ...
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bioRxiv - Genetics 2019Quote: ... We PCR-amplified the above sequence with stop codon and assembled it into a lentiviral plasmid (Addgene #85969) with either EF1α promoter (Addgene # 11154 ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) or amplified by PCR with overhangs containing BpiI (BbsI) recognition sites and assembled into pUAP1 (Addgene #63674) to create parts compatible with the phytobrick standard (3) ...
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Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extensionbioRxiv - Developmental Biology 2021Quote: ... the full RhoGEF2 cDNA of Drosophila melanogaster was amplified by PCR from plasmid UAS-akkordion dNhe (Addgene 41976) (71) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1 ug total DNA (0.5 ug PCR product and 0.5 ug AsCpf1_TATV Cas12 [Addgene: 89354]) using Xtreme-GENE 9 following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the cDNA of human CAV1 was PCR amplified from the mammalian expression plasmid Emerald-CAV1 C-10 (Addgene No ...
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bioRxiv - Molecular Biology 2021Quote: ... and miRFP2 genes were PCR amplified and swapped with mClover2 in pmClover2-tubulin-C-18 plasmid (Addgene #56376) and with TagRFP675 in pTagRFP675-actin-C1 (Addgene #44277 ...
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bioRxiv - Immunology 2020Quote: Soluble human ACE2 with an Fc tag was constructed by PCR amplifying ACE2 (residues 1-615) from Addgene plasmid #1786 (a kind gift from Jesse Bloom ...
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bioRxiv - Cell Biology 2022Quote: ... SA-KDEL-IRES and SBP PCR fragments were separately amplified from Str-KDEL_SBP-EGFP-GPI (gift from Franck Perez, Addgene plasmid #65294; RRID:Addgene_65294)28 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Genetics 2022Quote: ... The PCR product was cloned via Gibson assembly into AgeI and EcoRI digested pLKO.1 vector (Addgene#8453). Ligated libraries were electroporated into DH5a electrocompetent cells (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... the Adamts2 or TGFβR2 coding sequence was amplified by PCR using mouse Adamts2 and TGFβR2 ORF plasmids (pCMV-Adamts2, Harvard plasmid clones; pCMV-TGFβR2, Addgene) as templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... we transfected 1μg of PCR-amplified dsDNA reporter into naïve mESCs together with spCas9 plasmid pX459 (Addgene #62988), carrying a single gRNA complementary to the genomic integration site ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs ...
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bioRxiv - Cell Biology 2022Quote: Plasmids encoding GFP-2A-ORF6 were generated by inserting EGFP-T2A sequence (copied from Addgene #140424 by PCR) at the 5’ end of the ORF6 gene in pSecTag2 mammalian expression vector using Gibson Assembly ...
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bioRxiv - Molecular Biology 2023Quote: The DNA fragment encoding dLwaCas13a was PCR-amplified using pC035-dLwaCas13a-msfGFP (a gift from Feng Zhang; Addgene plasmid #91925 ...
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bioRxiv - Plant Biology 2023Quote: ... Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751 ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...