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Citations for Addgene :
51 - 100 of 959 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human Sin1.1 was PCR-amplified from Addgene 73388 plasmid (gift from Taekjip Ha32 ...
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bioRxiv - Cell Biology 2021Quote: ... Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135 ...
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bioRxiv - Molecular Biology 2022Quote: The Dux promoter was amplified by PCR with PCR mix (Yeasen, 10149ES03) and then cloned into pGL4.10[luc2] (Addgene, E6651) vector ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments amplified from pFLAG-attP (Addgene, #110095) with primers Apr fw/Apr rv and from pSEVA524 with primers tetA fw/tetA rv,25 respectively ...
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bioRxiv - Genetics 2022Quote: ... The TDH3 promoter was PCR-amplified from Addgene plasmid #67639 (a gift from John Wyrick) ...
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bioRxiv - Genetics 2022Quote: ... We PCR amplified the NatMX cassette from Addgene plasmid #35121 using primers with homology to the 5’ upstream and 3’ downstream sequences of the targeted gene ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product and pCS2-FLAG (AddGene 16331) were digested with EcoRI and XhoI and ligated with T4 DNA Ligase ...
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bioRxiv - Cell Biology 2022Quote: ... A PCR product of ColX (from Addgene #110726) and a synthetic gene containing PAUF (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were cloned into pRAV23 (Addgene) using EcoRI and HindIII restriction sites and transformed into Top10 cells ...
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bioRxiv - Cell Biology 2020Quote: ... a PCR product of myc-BirA* (35700; Addgene) was ligated into XhoI and SpeI cleaved pCAG-mNCad-HA ...
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bioRxiv - Microbiology 2022Quote: ... which was PCR-amplified from pSFGFP-N1 (Addgene) [55].
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bioRxiv - Cell Biology 2022Quote: ... The iRFP ORF was PCR amplified from Addgene plasmid #45457.
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bioRxiv - Cancer Biology 2023Quote: ... HOXB13 was PCR amplified from pLX302_HOXB13 (Addgene #70089), digested with SalI/XbaI and ligated into linearized pENTR1A (Addgene #17398) ...
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bioRxiv - Neuroscience 2023Quote: ... Gamillus obtained by PCR reaction from (Addgene #124837) was inserted in place of SEP with a use of NEBuilder HiFi DNA Assembly ...
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bioRxiv - Developmental Biology 2024Quote: ... was PCR amplified from pJW2171 (Addgene plasmid #163095) and concentrated using a PCR purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2024Quote: ... A genetically encoded calcium indicator for the detection of ACh activity (1 μL, AAV9-hSyn-ACh3.0; AddGene; Watertown, MA) was infused into the medial prefrontal cortex (AP(2.7mm) ...
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bioRxiv - Genetics 2021Quote: ... DNA templates for PCR-IVT were produced using overlapping oligonucleotides in a high-fidelity PCR reaction47 or using a plasmid template (Addgene #4223048) and appropriate primers46 ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was used as a template for PCR and 2500ng of purified HDR template PCR product combined with 1500ng of guide RNA expression plasmid (Addgene 49330) containing the guide RNA listed in Table S4 were co-transfected into OSCs ...
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bioRxiv - Biophysics 2021Quote: ... for the live-cell time-lapse experiment and with pcDNA3.1(+)eGFP (Addgene plasmid #129020) for the FFS measurements ...
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bioRxiv - Genomics 2023Quote: ... We further replaced the Cas9 cassette with an nCas9/M-MLV-RT cassette from the pCMV-PE2 plasmid (Addgene, 132775). The lentiV2-pegRNA and lentiV2-ngRNA plasmids were constructed by replacing the Cas9 and Puromycin sequences in the lentiCRISPR v2 plasmid (Addgene ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were ligated into lentiCRISPR v2 (Addgene, 52961) using Gibson Assembly® Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... mCherry was PCR amplified from Cas9-mCherry (Addgene #78313) and cloned into the BamHI site ...
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bioRxiv - Cancer Biology 2020Quote: ... which was PCR-amplified from pEMS1384 (Addgene Plasmid #29304). The pHes5-d2EGFP plasmid was a gift from Ryoichiro Kageyama (Ohtsuka et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplified and cloned into lentiCRISPRv2 (Addgene Plasmid #52961) and lentiGUIDE-puro (Addgene Plasmid #52963 ...
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bioRxiv - Neuroscience 2020Quote: ... we first PCR amplified the OXTR sequence (Addgene, #66467) to contain ClaI restriction enzyme sites at each end ...
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bioRxiv - Neuroscience 2020Quote: ... the human ACE2 ORF was PCR amplified from Addgene plasmid 1786 and C-terminally fused with the porcine teschovirus-1-derived P2A cleavage sequence (ATNFSLLKQAGDVEENPGP ...
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bioRxiv - Biophysics 2020Quote: Full length HP1α was amplified by PCR (Addgene 17652), and cloned using InFusion kit into a pHR lentiviral vector under an SFFV promoter and tagged C-terminally with mCherry and sspB ...
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bioRxiv - Cell Biology 2022Quote: ... mApple and sfCherry2 cDNAs were PCR-amplified from Addgene plasmids #54862 and #83031 ...
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bioRxiv - Molecular Biology 2022Quote: ... one obtained by PCR on pRPR1_gRNA_handle_RPR1t (Addgene Plasmid #49014) using OFS_2869 and OFS_2870 oligonucleotides ...
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bioRxiv - Neuroscience 2020Quote: ... Postsynaptic mouse neuroligin-1 was PCR amplified from Addgene #15260 ...
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bioRxiv - Biochemistry 2021Quote: A PCR product from plasmid pDD282 (Addgene plasmid # 66823) was used as a donor template for insertion of gfp ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were subcloned into the pUC19 plasmid (Addgene) and sequenced.
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bioRxiv - Molecular Biology 2022Quote: ... PUM1 was PCR amplified from pMAL-PUM1 (Addgene #120384) and PUM2 was PCR amplified from pMAL-PUM2 (Addgene #120385) ...
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bioRxiv - Microbiology 2020Quote: ... the human hACE2 ORF was PCR amplified from Addgene plasmid 1786 and C-terminally fused with the porcine teschovirus-1-derived P2A cleavage sequence (ATNFSLLKQAGDVEENPGP ...
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bioRxiv - Neuroscience 2020Quote: ... we PCR-amplified the CEPIA coding region from Addgene plasmids pCMV CEPIA3mt and pCMV CEPIA4mt (Suzuki et al ...
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bioRxiv - Genomics 2022Quote: ... The PCR primers and conditions are available from Addgene. The PCR product were mixed and cleaned using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... KAP1 was PCR amplified from pEGFP-KAP1 (Addgene, #45568) and inserted in place of PMLIII into pBOS-PMLIII-YFP plasmid.
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bioRxiv - Genetics 2022Quote: ... Gata6 cDNA was PCR amplified from pSAM2_mCherry_Gata6 (Addgene #72694) and ligated using InFusion cloning.
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bioRxiv - Systems Biology 2024Quote: ... PpHIS4 gene was PCR amplified from pMJA089 (Addgene #128518) (Yang et al. ...
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bioRxiv - Cell Biology 2021Quote: ... at which time they were transfected with 5μg of Cre-ire-puro vector (Addgene #30205) along with 3μg pAX2 and 2μg VSVG packaging plasmids using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: We constructed a vector (sgOpti-HyPR) capable of expressing both a gRNA and a “detection barcode” by modifying sgOpti (Addgene 85681) to insert a 400 bp fragment between the puromycin resistance cassette and WPRE ...
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bioRxiv - Developmental Biology 2020Quote: ... New fasta and annotation files were created using mkref in mm10 for the detection of the H2B::GFP sequence (Addgene #11680). CellRanger count function was used to assign reads to genomic features ...
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bioRxiv - Genetics 2023Quote: ... by performing gibson assembly with the TRE3G promoter and P2A-BFP-WPRE amplified from TRE-KRAB-dCas9-IRES-BFP (addgene #85449) combined with nCas9(H840A)-MMLV(RT) amplified from pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE (Addgene# 20945) and 7.3Kb (lentiviral backbone ...
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bioRxiv - Synthetic Biology 2023Quote: ... The YTK kit (Addgene; Kit #1000000061) was used as a source for all relevant non-coding DNA sequences — including promoters and terminators ...
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bioRxiv - Molecular Biology 2020Quote: ... crassa genomic DNA with primers 598 and 599 by PCR and inserting the PCR product into the SpeI site of Plasmid 43802 (Addgene, DiCarlo et al. 2013). The resulting plasmid was used as the template for PCR-based amplification of tef-1(P)-cas9Hs with primers 610 and 589 ...
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bioRxiv - Cell Biology 2020Quote: ... We PCR amplified d2EGFP from M38 TOP-dGFP (Addgene #17114), engineering XhoI and NotI sites using primer sequences 5’-AAACTCGAG-GCCACCATGGTGAGCAAGG and 5’-AAAGCGGC-CGCCTACACATTGATCCTAGCAGAAG and cloned the coding region upstream of the β-globin-UTR ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR product was then subcloned into pcDNA3.1+ (Addgene) using BamHI and NotI restriction endonucleases (pcDNA3.1+-KanSacB).
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bioRxiv - Developmental Biology 2021Quote: Kaede PCR product was amplified from the plasmid (Addgene #54726) for generating the template for capped mRNA synthesis (primers F ...
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bioRxiv - Microbiology 2021Quote: ... were PCR amplified and inserted into the plasmid pFGL1010 (Addgene, 119081 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR amplified and cloned into lentiGuide-Puro vector (Addgene #52963). Briefly ...