Labshake search
Citations for Addgene :
601 - 650 of 957 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... a fragment containing the GIGYF2 sequence was obtained by PCR from the pcDNA4/TO/GFP-GIGYF2 vector (Addgene plasmid 141189) (34 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Amplified PCR products were fused to biotin ligases via In-Fusion Recombination into myc-BioID2 pBabe (Addgene #80900; XhoI/PmeI), BioID2-HA pBabe (Addgene #120308 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sox2-t2A-mCherry was digested with XbaI and NheI to remove Sox2 and replace with PCR products from Nanog (Addgene plasmid # 59994 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sox2-t2A-GFP was then cut with KpnI and AgeI to remove GFP and replace with PCR-amplified mCherry from pAAVS1-NDi-CRISPRi (Addgene plasmid #73497 ...
-
bioRxiv - Cell Biology 2019Quote: ... the GFP coding sequence was amplified by PCR and inserted into pRS406-ADH1 (p406ADH1 was a gift from Nicolas Buchler & Fred Cross-Addgene plasmid # 15974 ...
-
bioRxiv - Molecular Biology 2019Quote: ... cerevisiae Sth1p bromodomain (residues 1250-1359) was generated by PCR from genomic DNA and cloned into a modified pGEX-4T1 vector (Addgene) using Ligation Independent Cloning (LIC) ...
-
bioRxiv - Cell Biology 2019Quote: The mCherry-DNKASH construct was made from the DNKASH-TSMod construct digested by AgeI/HpaI and mCherry from a PCR on a mCherry-cSrc construct (from M. Davidson, Florida State University, Tallahassee, FL; 55002; Addgene) (fwd ...
-
bioRxiv - Cell Biology 2021Quote: ... Source of different elements are as follows: LAMP1 signal peptide and human LAMP1 were PCR amplified from LAMP1-mGFP (Addgene Plasmid #34831 ...
-
bioRxiv - Cell Biology 2020Quote: ... plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).
-
bioRxiv - Bioengineering 2020Quote: ... and mRuby2-tagged Lifeact were constructed by inserting the PCR-amplified cDNAs (human TAGLN2, pFN21ASDA0120, Kazusa DNA Research Institute; Lifeact, Addgene plasmid # 54688 ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR amplified products were cloned into BsmB1-digested LRG2.1 (a lentiviral sgRNA expression vector, U6-sgRNA-EFS-GFP, Addgene: 108098) using the Gibson Assembly kit (NEB#E2611) ...
-
bioRxiv - Cancer Biology 2019Quote: ... CDKN1A and NRF2 PCR amplicons were prepared with SpeI and MfeI restriction sites and cloned into pEN_TTmiRc2 BirA* (Addgene #136521). Luciferase PCR amplicon was prepared with SpeI and EcoRI restriction sites and cloned into pEN_TTmiRc2 3xFLAG digested with SpeI and MfeI sites (Addgene #136519) ...
-
bioRxiv - Cancer Biology 2019Quote: ... and p53(R280K)-V5 PCR amplicon was prepared with SpeI and MfeI restriction sites and cloned into pEN_TTmiRc2 (Addgene #25752) digested with SpeI and MfeI (Addgene #136520 ...
-
bioRxiv - Neuroscience 2020Quote: ... BirA-HA and HaloTag constructs were PCR-amplified from pcDNA3.1-MCS-BirA(R118G)-HA (a gift from Kyle Roux, Addgene #36047) (Roux et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by digestion of the PCR product with EcoRI and SacII and ligation in between Smoothened and mCherry in mCherry-Smo (Addgene plasmid 55134 ...
-
bioRxiv - Cell Biology 2021Quote: GCaMP6m was generated by PCR amplifying the GCaMP6m sequence from pGP-CMV-GCaMP6m (Addgene plasmid #40754, (Chen et al., 2013)) and ligating it into the BamH1 and EcoR1 sites in pCS2+ ...
-
Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The CyPet gene was amplified by PCR from the pCyPet-His vector (pCyPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14030 ...
-
Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The YPet gene was amplified by PCR from the pYPet-His vector (pYPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14031 ...
-
bioRxiv - Neuroscience 2020Quote: ... The entire coding sequence of GCaMP6s was amplified by PCR with primers 5’–GGATCCGCCACCATGGGTTCTCATCATCATCA–3’ and 5’–GTAGCCGAACCGGTCTTCGCTGTCATCATTTGTAC–3’ using pGP-CMV-GCaMP6s (Addgene plasmid # 40753 ...
-
bioRxiv - Neuroscience 2020Quote: ... and jRGECO1a(Dana et al. 2016) were first amplified by PCR from pGP-CMV-GCaMP6f and pGP-CMV-NES-jRGECO1a (Addgene) with 5’ BamHI and 3’ HindIII ...
-
bioRxiv - Microbiology 2020Quote: PCR fragments corresponding to 231 nts of the Citrine coding sequence were amplified from ES-FUCCI plasmid (Addgene plasmid#62451) using primers containing T7 promoter sequence with 2 distinct PCR fragment allowing the positive-sense or negative-sense RNA ...
-
bioRxiv - Neuroscience 2020Quote: ... The loxP-NeoR-loxP cassette was inserted at the MluI site in the intron between exons 1 and 2 following PCR amplification from pL452 (Addgene) using primers 3-For and 3-Rev ...
-
bioRxiv - Molecular Biology 2022Quote: ... using the overlapping PCR fragment method described in (Friedland et al. 2013) or were cloned into pDD162 (Addgene plasmid #47549) using the Q5 Site Directed Mutagenesis Kit (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2019Quote: ... the p300 HAT core domain (p300 core) was PCR amplified from the pcDNA-dCas9-p300-Core vector (Addgene, Plasmid #61357) and cloned into MluI/BstXI digested pHR-TRE3G-KRAB-dCas9-P2A-mCherry backbone ...
-
bioRxiv - Molecular Biology 2019Quote: ... the KRAB sequence was PCR amplified from the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2A-Puro vector (Addgene, plasmid #71236) and cloned into the enCRISPRa sgRNA vector to replace VP64 ...
-
bioRxiv - Cell Biology 2019Quote: ... IRES DNA and mCherry cDNA were prepared by PCR using pEYFP SUMO-1 plasmid (from Mary Dasso: Addgene plasmid #13380), pWPI plasmid (from Didier Trono ...
-
bioRxiv - Synthetic Biology 2020Quote: Plasmids expressing dCas9-VPR were constructed by Isothermal assembly34 combining NotI linearized pMBO2744 attP vector backbone with dCas9-VPR PCR amplified from pAct:dCas9-VPR (Addgene #78898)35 and SV40 terminator for pH-Stinger (BDSC ...
-
bioRxiv - Cell Biology 2020Quote: ... we amplified two DNA fragments with PCR and used Gibson assembly to subclone these into the BamHI site of pBluescript II KS+ (Addgene). The first fragment ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293Ts were made to stably express doxycycline(dox)-inducible wild-type muSOX and its catalytically-dead D219A mutant by PCR amplifying the aforementioned coding sequences from Addgene plasmids 131702 and 131704 using the muSOX F/R primers (see Key Resources Table ...
-
bioRxiv - Synthetic Biology 2020Quote: ... moonbody (S5.1) cDNA was amplified via PCR and then inserted into the pSH-EFIRES-P-AtAFB2-mCherry vector (Addgene, #129716) between EcoRI and NotI sites to replace mCherry.
-
bioRxiv - Cell Biology 2021Quote: ... pRS315-NOP1pro-GFP1-10-mCherry-PUS1 and pRS315-NOP1pro-GFP1-10-mCherry-SCS2TM were generated using PCR products amplified from Addgene plasmids #86413 and #86419 and Addgene plasmids #86416 and #86419 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the genes of NIR-GECO(1,2,2G)-T2A-GFP and were constructed using overlap-extension PCR followed by subcloning into pAAV-CAG vector (Addgene no. 108420) using BamHI and EcoRI sites ...
-
bioRxiv - Neuroscience 2020Quote: ... DNA fragment of Cre was prepared by PCR and transferred to retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... pHCKan-yibDp-CBD-hGLY was constructed by DNA assembly with 2 PCR products amplified from (i) a plasmid coding hGLY under a T7 promoter (pHCKan-T7-CBD-hGLY, Addgene #134940 ...
-
bioRxiv - Genetics 2020Quote: ... of homology arms (∼400bp) PCR amplified from genomic DNA and the FKBP12F36V sequence amplified from plasmid pLEX_305-N-dTAG (Addgene # 91797) (Nabet et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... The constitutively active form of kinesin-1 (human KIF5B, K560) was cloned by PCR from pet17-K560-GFP_His (Addgene, 15219) into pEGFP-N1 vector using EcoRI primer (104-5p EcoRI-K560 ...
-
bioRxiv - Cell Biology 2021Quote: ... EGFP-Miro2 and mCherry-Miro1 were generated by PCR amplification of the coding sequence of Miro and ligated into pEGFP-C1(Addgene) or pmCherry-C1(Addgene) ...
-
bioRxiv - Developmental Biology 2020Quote: ... a FLAG-tag version of the codon-optimized mouse DUX was amplified by PCR (Primers in Extended Table 1) from pCW57.1-mDUX-CA (Addgene 99284) and subcloned into the pBS31 plasmid (pBS31-FLAG_mDUX) ...
-
bioRxiv - Biochemistry 2021Quote: ... the vector backbone was PCR-amplified and the resulting fragment was ligated with the ccdB kill-cassette excised from pINIT_cat (Addgene #46858) with SapI ...
-
bioRxiv - Cell Biology 2021Quote: ... T7 promoter was added to the Cas9 coding region and sgRNA-scaffold by PCR amplification of plasmid PX330 (Addgene, #42230). PCR products of Cas9 and sgRNA region were used for IVT by following the manufactural of mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies ...
-
bioRxiv - Biophysics 2020Quote: ... A cpGFP cassette was amplified by PCR from a pCDNA3.1 plasmid encoding ASAP1 (a gift from Dr. Michael Lin, Stanford, available as Addgene #52519) and inserted into pCDNA3.1-GPR68 using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
-
bioRxiv - Genomics 2021Quote: The lentiviral dCas9 expression plasmid (lenti-dCas9-Blast) was generated by PCR-based mutagenesis of lentiCas9-Blast plasmid (Addgene #52962). Clover fused with PUF RNA-binding domain were previously described ...
-
bioRxiv - Cell Biology 2021Quote: The EB1-GFP lentivirus expression construct was made by PCR amplification of EB1-GFP from pEGFP N1 EB1-GFP (JB131) (gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39299 ...
-
bioRxiv - Neuroscience 2020Quote: All constructs containing the N/Q-rich region of the neuronal-specific Aplysia CPEB (residues 1-160) were cloned by PCR using a full-length clone (Addgene) as the template ...
-
bioRxiv - Molecular Biology 2021Quote: ... and its RdRp domain (aa 91-610) were obtained using PCR on a SINV cDNA plasmid template (pSIN ReP5-GFP-GluR1 from Addgene). The PCR fragments were subcloned into the pSUMO-LIC vector using a ligation-independent cloning method in which the expressed recombinant protein had an N-terminal hexahistidine-linked small ubiquitin-like modifier protein (His6-SUMO ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with two gRNA transcriptional cassettes prepared by PCR and CAG-Cas9-T2A-EGFP plasmid (Addgene #7831) as described elsewhere56 ...
-
bioRxiv - Biophysics 2022Quote: ... and 32C (residues 172–270 of RPA2) domains of human RPA were PCR amplified using p11d-tRPA plasmid (Addgene plasmid102613) (Henricksen et al. ...
-
bioRxiv - Bioengineering 2022Quote: ... After digestion with EcoRI and AgeI the PCR products were ligated into the pLVX-TetOne-Puro vector (Addgene plasmid #124797).
-
bioRxiv - Cell Biology 2022Quote: The mat-Gal4-sqhUTR plasmid was generated by PCR amplification of the Gal4 coding sequence from pPac-Pl-mCD8-D2A-Gal4 (a gift from Benjamin White, Addgene plasmid # 39457 ...
-
bioRxiv - Physiology 2022Quote: ... an mCherry-T2A-hM4Dinrxn cassette was PCR-amplified using CAG::mCherry-2a-hM4Dnrxn (Addgene #52523, donated by Dr. Scott Sternson) as a template with primers containing 5’-BamHI site and 3’-MluI site and then inserted into pENTR1A-TRE ...