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Citations for Addgene :
1 - 50 of 81 citations for pcDNA5 FRT since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA5-FRT-TO-EGFP-AID (Addgene, 80075) was used as template for both the EGFP-AID and EGFP tags ...
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bioRxiv - Cell Biology 2020Quote: pcDNA5/FRT/TO-Puro was purchased from Addgene. PCR was used to generate the following GFP fusion proteins ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA5-FRT-TO-FH-Nsp2 (Addgene plasmid 157683) was used ...
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bioRxiv - Molecular Biology 2021Quote: A pcDNA5/FRT/TO GFP (ITEM 19444) was purchased from Addgene. PCR was used to amplify all the different KRAS (WT ...
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA5-FRT-TO-EGFP-AID was a gift from Andrew Holland (Addgene plasmid # 80075 ...
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bioRxiv - Cancer Biology 2021Quote: ... The pcDNA5-FRT/TO-LacR-FLAG-TOPBP1 plasmid was obtained from Addgene (#31313). Point mutants were introduced by site-directed mutagenesis using Quikchange (Agilent) ...
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bioRxiv - Cell Biology 2020Quote: ... . pCDNA5/FRT EGFP-miniAID was a gift from Andrew Holland (Addgene plasmid # 101714). pBabe Blast osTIR1-9myc was a gift from Andrew Holland (Addgene plasmid #80073) ...
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bioRxiv - Cell Biology 2024Quote: The expression plasmids pCDNA5 FRT/TO-NeoR-HA-VPS35 WT and [D620N] were generated by subcloning either pcDNA5D-FRT/TO-HA-VPS35-WT (MRC PPU; DU26467) or pcDNA5D-FRT/TO-HA-VPS35-D620N (MRC PPU; DU26878) into the vector pcDNA5/FRT/TO-neo (Addgene; 41000). Prior to transfection ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA5 FRT/TO PLK4-mCherry (Moyer et al, 2015) was obtained from Addgene (#80269). Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... The pCDNA5 FRT/TO HA-eIF4G1 WT was created by subcloning HA-eIF4G1 from Addgene: 45640 via HindIII and XhoI into the same restriction sites of pCDNA5 FRT/TO ...
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bioRxiv - Molecular Biology 2023Quote: ... pET41b+_Npl4 (#117108) and pcDNA5/FRT/TO-FAF1-Strep-HA (#113486) were acquired from Addgene. Ubiquitin binding-deficient (*UBD ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA fragment encoding APEX2 was PCR amplified from pcDNA5/FRT/TO APEX2-GFP (Addgene) and fused to the N-terminus of DDX3X using fusion PCR ...
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bioRxiv - Cell Biology 2020Quote: ... BioID2-RPTOR-pcDNA5 FRT/TO was created by shuttling RPTOR from R77-E301 Hs.RPTOR (70585, Addgene) into BioID2-pcDNA5 FRT/TO using Gateway LR Clonase II enzyme mix (11791100 ...
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bioRxiv - Cell Biology 2020Quote: ... BioID2-pcDNA5 FRT/TO was created by restriction digestion of BioID2 from myc-BioID2-MCS (74223, Addgene) using NheI and XhoI ...
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bioRxiv - Immunology 2021Quote: ... gene sequence was subcloned from pcDNA5/FRT/TO HIS HSPA1A (a gift from Harm Kampinga, Addgene plasmid # 19537; http://n2t.net/addgene:19537; RRID:Addgene_19537)(46 ...
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bioRxiv - Neuroscience 2021Quote: hM4D(Gi) was amplified by PCR from pcDNA5/FRT-HA-hM4D(Gi) (Addgene #45548 (Armbruster et al., 2007)) using forward primer ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA5 FRT TO myc hSeparase was a gift from Stephen Taylor (Holland & Taylor, 2006) (Addgene plasmid number #59820) the mutants (LD ...
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bioRxiv - Immunology 2021Quote: ... The human full length (HFL) gene sequence was subcloned from pcDNA5/FRT/TO HIS HSPA1A (a gift from Harm Kampinga, Addgene plasmid # 19537 ...
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bioRxiv - Cell Biology 2022Quote: ... The cell line inducibly expressing Flag-NSP2 was generated by co-transfecting pcDNA5-FRT-TO-FH-Nsp2 (Addgene plasmid 157683) and pOG44 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... a pooled library of synthetic 3’ UTRs was cloned downstream of eGFP in the pCDNA5/FRT/TO plasmid (Addgene 19444). Each 3’ UTR consisted of a 19-nucleotide fixed spacer ...
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bioRxiv - Molecular Biology 2020Quote: ... first pcDNA5 PURO FRT TO EGFP-AID-CENATAC was created by cloning CENATAC cDNA derived from HeLa cells into empty pcDNA5-FRT-TO-EGFP-AID (Addgene, 80075) using the cDNA PCR primers in Table S9 and digestion of both the PCR product and the plasmid with NotI/ApaI ...
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bioRxiv - Cell Biology 2020Quote: ... with oligos 5’-CCCaagcttACCATGGACTATAAGGACGATGATGACAAAGAACACCAGCTCC-3’ and 5’-CCCaagcttCTCCGGATTACCTTCATCCTTATGTAGATAAGAGTGCTGCAG-3’ and cloned into the HindIII site in frame upstream of pcDNA5 FRT/TO 3xHA-Venus-1xminAID (Daniel et al., 2018) (identical to Addgene #117714) but with a neomycin instead of hygromycin resistance ...
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bioRxiv - Cell Biology 2023Quote: ... The KT binding domain sequence of 53BP1 (aa 1235-1616) was PCR-amplified from pcDNA5-FRT/TO-eGFP-53BP1 (Addgene plasmid #60813) and cloned into pB66 downstream to the Gal4 DNA-binding domain ...
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bioRxiv - Molecular Biology 2024Quote: We reconstituted RIF1-/- U-2 OS cells with full-length RIF1-L and RIF1-S coding sequences (CDS) cloned into a tetracycline-inducible pcDNA5-eGFP-FRT/TO plasmid vector (Addgene plasmid #19444) by Gateway recombination cloning (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... The brdisc-FRT-tdTomato-2xSTOP-FRT-myrGFP (brdisc-switch) reporter was generated from pJFRC177 10xUAS-FRT-2xSTOP-FRT-myrGFP (Addgene 32149). The brdisc enhancer was restriction cloned into the HindIII and AatII sites ...
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bioRxiv - Cell Biology 2021Quote: pG-LAP1 (pCDNA5/FRT/TO-EGFP-TEV-Stag-X) and pG-LAP5 (pEFα-X-Stag-PreScission-EGFP) were from Addgene (Torres et al., 2009). pENTR223-ARL13B fusion construct was from DNASU (HsCD00511796) ...
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bioRxiv - Developmental Biology 2021Quote: ... A template plasmid (p-sfGFP-FRT-amp-FRT, Addgene #176620) was constructed by inserting a gBlock encoding sfGFP followed by an SV40 polyA sequence and a FRT-flanked ampicillin cassette in place of EGFP in the pEGFP-C1 plasmid (Clontech) ...
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bioRxiv - Molecular Biology 2024Quote: ... and pcDNA5-H2B_Halo_T2A_EGFP (Addgene:135444) respectively (Frei et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 (92140, Addgene) and the plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (42330 ...
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bioRxiv - Developmental Biology 2022Quote: ... an FRT-stop-FRT3-FRT segment was cloned from the CoinFlp plasmid97 (Addgene plasmid # 52889 ...
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bioRxiv - Cancer Biology 2023Quote: ... “act-FRT-stop-FRT-siSpt5” was generated by inserting “AggccagtCAGAAGCTACAGTCCATTCAAtagttatattcaagcataTTGAATGGACTGTAG CTTCTGgcggccAGTC” (“siSpt5_f”) into pAct-FRT-stop-FRT3-FRT-FRT3-GAL4_attB (AddGene vector #52889; Bosch et al, 2015). The resulting construct pACT5C-FRT-stop-FRT-siSpt5 was inserted in ZH86Fb by GenetiVision Corp (Houston ...
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bioRxiv - Developmental Biology 2020Quote: ... pcDNA5-EGFP-AID-BubR1 (Addgene #47330), followed by mutation of the MAO binding site using the Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Biophysics 2024Quote: ... pcDNA5-Flag-BRD4-WT (Addgene Plasmid #90331)
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bioRxiv - Biophysics 2024Quote: ... pcDNA5-Flag-BRD4-7A (Addgene Plasmid #90006), pcDNA5-Flag-BRD4-7D (Addgene Plasmid #90007) ...
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bioRxiv - Biophysics 2024Quote: ... pcDNA5-Flag-BRD4-7D (Addgene Plasmid #90007), pcDNA5-Flag-BRD4-BD (Addgene Plasmid #90005) ...
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bioRxiv - Biophysics 2024Quote: ... pcDNA5-Flag-BRD4-BD (Addgene Plasmid #90005), pcDNA5-Flag-BRD4-WT (Addgene Plasmid #90331)
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bioRxiv - Neuroscience 2024Quote: We amplified the flp-out cassette from pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG53 (Addgene #63169) via PCR and integrated it into Dsec1029-mhc-YFP via Gibson assembly resulting in Dsec1029-flip-out-mhc-YFP ...
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bioRxiv - Neuroscience 2020Quote: ... the FRT-stop-FRT cassette (
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bioRxiv - Physiology 2021Quote: ... in a Flex(FRT) cassette (using Addgene plasmid #55641 ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV-EF1α-FRT-FLEX-MCS (Addgene #190771) was generated by replacing the DIO-oChIEF(E163A/T199C)-P2A-dTomato of pAAV-EF1α-DIO-oChIEF(E163A/T199C)-P2A-dTomato with the FRT-FLEX-MCS of pJ244-FRT-FLEX-MCS {Xue:2014dl} ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV-EF1α-FRT-FLEX-EGFP (Addgene #190770) was generated by replacing the mNaChBac-T2A-tdTomato of pAAV-EF1α-FRT-FLEX-mNaChBac-T2A-tdTomato with the EGFP from pCAG-EGFP ...
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bioRxiv - Synthetic Biology 2021Quote: ... while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213925) was used as template for both hGeminin and hCdt1 tags ...
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bioRxiv - Cell Biology 2020Quote: ... and mCCND1-CDK4-mVenus was inserted into the FRT site of RPE-1 FRT/TO mRuby-PCNA expressing in addition ectopic pCAGGs-NLS-TIR1_P2A_NES-TIR1 (Addgene: 117699). pCAGS-myc-BirA-P2A-3xflag-Avitag-UFM1-IRESpuro3 was electroporated into RPE-1 H3.1-mTurquoise2 + mRuby-PCNA UFM1 knockout cells followed by selection for stable integrands expressing 3xflag-Avitag-UFM1 to the same level as endogenous UFM1 (see Figure S4D ...
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Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor nichebioRxiv - Developmental Biology 2022Quote: ... Rubin) was used to replace the pAct of the pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB vector (Addgene #52889). To make pBP-htl-enh-nlsLexA::p65Uw (htl-LexA) ...
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Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor nichebioRxiv - Developmental Biology 2022Quote: ... The T2A-nls:Gal4:VP16-STOP sequence was generated by PCR (Supplementary Table 3) from the pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB vector (Addgene). 5’HA-T2A-nls:Gal4:VP16-STOP-3’HA was assembled in correct 5’-to-3’ order between Not1 and EcoR1 sites of the pJet1.2 vector.
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bioRxiv - Developmental Biology 2020Quote: ... R1 and ESCDUX were co-transfected using jetPRIME (PolyPlus transfection) with the plasmids CTCF-AID[71-114]-eGFP-FRT-Blast-FRT (92140, Addgene), pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26 (92140 ...
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bioRxiv - Neuroscience 2022Quote: ... The iCre-P2A-3xNLS-dTomato from pAAV-CaMKIIα-iCre-P2A-3xNLS-dTomato was then inserted into pAAV-EF1α-FRT-FLEX-MCS to generate pAAV-EF1α-FRT-FLEX-iCre-P2A-3xNLS-dTomato (Addgene #190772). pAAV-EF1α-FRT-FLEX-iCre-P2A-3xNLS-mNeonGreen (Addgene #190773 ...
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bioRxiv - Neuroscience 2024Quote: ... mScarlet-FRT and mGreenLantern-FRT plasmids were generated by subcloning the mScarlet and mGreenLantern coding sequence (Addgene #85042 and #164468, respectively) inside the FRT-flanking region of a CAG-promoter plasmid (Addgene #99133) ...
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bioRxiv - Neuroscience 2024Quote: ... DsecattP40-flip-out-3xP3-DsRed was generated by PCR amplification of pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-HA53 (Addgene #63166) and integration into Dsec-attP40-3xP3-DsRed.
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bioRxiv - Biochemistry 2021Quote: ... spike was cloned into a pcDNA5-based vector (Addgene #113547) with the following additional N-terminal Ig-Kappa leader and C-terminal epitopes ordered as two separate gBlocks (IDT) ...