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Citations for Addgene :
51 - 78 of 78 citations for p p' DDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 15 μg of pMDLg/p RRE and 10 μg of pRSV-Rev packaging plasmids (Addgene #12251 and Addgene #12253) and the pVSV envelope plasmid (Addgene #8454 ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg of pMDLg/p RRE and 10 μg of pRSV-Rev packaging plasmids (Addgene #12251 and Addgene #12253) and the pVSV envelope plasmid (Addgene #8454 ...
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bioRxiv - Genetics 2023Quote: ... a pDestTol2CG2-eye-bfp with the independent marker beta-crystaline:BFP a kdrl P-5’entry clone (Addgene, Santoro Lab), an EGFP-CAAX p-middle entry (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: 20 µg of plasmid p-dCas9-LSD1-Hygro (a gift from Stephan Beck and Anna Koeferle, available through Addgene plasmid #104406 ...
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bioRxiv - Cell Biology 2024Quote: ... to introduce negative selection cassettes flanking the AAVS1 homology arms from the Gateway-compatible pAAVS1-P-CAG-DEST vector from Knut Woltjen (RRID:Addgene_80490). The resulting vector (pAAVS1-CAG-DEST-DTA-TK ...
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bioRxiv - Biochemistry 2020Quote: ... Seamless cloning was used to insert His6-EF-P into a pDSG310 vector (a gift from Ingmar Riedel-Kruse: Addgene plasmid #115611 ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse Halo-FMRP construct was generated by replacing the EGFP with the HaloTag coding sequence in the p-EGFP-C1-Flag-mFmr1(wt) vector which was a gift from Stephanie Ceman (Addgene plasmid #87929 ...
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bioRxiv - Synthetic Biology 2020Quote: ... moonbody (S5.1) cDNA was amplified via PCR and then inserted into the pSH-EFIRES-P-AtAFB2-mCherry vector (Addgene, #129716) between EcoRI and NotI sites to replace mCherry.
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bioRxiv - Neuroscience 2022Quote: ... pAAV-Per2-intron2-Venus-NLS-D2 was generated by deleting the LoxP cassettes of pAAV-P(Per2)-DIO-intron2-Venus-NLS-D2 (Addgene). The AAV vectors were produced by a procedure as described previously (41) ...
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bioRxiv - Neuroscience 2022Quote: pAAV-Bmal-forward-intron336-Venus-NLS-D2 was generated by swapping the Cry1 promoter of pAAV-P(Cry1)-forward-intron336-Venus-NLS-D2 (Addgene) with a Bmal1 promoter (40) ...
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bioRxiv - Neuroscience 2023Quote: ... A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene).
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bioRxiv - Microbiology 2024Quote: Sequences of RhCMV vFcγRs were synthesized as gBlock fragments (IDT) and cloned via Pst1 and BamH1 digest into a p-IRES-eGFP vector (Addgene) providing a unified tapasin signal peptide and resulting in an N-terminal HA-epitope tag upon cleavage of the tapsin signal peptide (55) ...
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bioRxiv - Developmental Biology 2019Quote: ... using AscI and NotI-containing primers and cloned the fragment into the vector p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open] (Addgene plasmid #71809). Transgenic lines were generated by Genetivision Inc ...
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bioRxiv - Cell Biology 2019Quote: ... The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397) as a gift from David Liu [31] ...
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bioRxiv - Systems Biology 2019Quote: P(dat-1)::YFP and P(dat-1)::alpha-Synuclein-YFP were generated by cloning the P(dat-1) promoter in the plasmids pRP2386 28 and pPD30.38 (Addgene plasmid # 1443) respectively ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... OsTIR1 and AtAFB2 plasmids were used (AtAFB2) (pMK232 CMV-OsTIR1-PURO, Addgene #72834; pSH-EFIRES-P-AtAFB2-mCherry-weakNLS, Addgene #129717) [3,10] ...
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bioRxiv - Cell Biology 2021Quote: ... a codon-optimized signal sequence from human ER-resident p23 was inserted into the AgeI site of p-sfGFP-N1 (Addgene #54737). p23ss-sfGFP lacking the stop codon was PCR amplified and flanked with a 3’ NheI site and TA cloned into pCRII-TOPO vector ...
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bioRxiv - Developmental Biology 2023Quote: ... iNPCs were transfected with a mixture of two plasmids that provide donor DNA for targeted knockin of CAG promoter-driven mCherry expression cassette at the AAVS1 loci (pAAVS1-P-CAG-mCherry, Addgene, # 80492), and that express Cas9 and sgRNA (pXAT2 ...
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bioRxiv - Biochemistry 2023Quote: ... The sense guide insert was subsequently cloned into BbsI digested pBABED P U6 (University of Dundee DU48788) and the antisense cloned into BbsI digested pX335 (Addgene #42335), yielding clones DU69746 and DU69747 respectively.
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bioRxiv - Biochemistry 2023Quote: ... Bpa-mutant Pet28n-6xHis-BRCA11-104-(GS)6-BARD126-221 plasmids were co- transformed with a pEVOL-pBpF plasmid encoding an Bpa aminoacyl tRNA synthetase (gift from P. Schultz, Addgene #31190) in E ...
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bioRxiv - Cell Biology 2024Quote: ... and ligated into the pre-digested promoter-less plasmid to generate the pAAVS1-P-CAG-mNG21-10 repair template (Addgene #206042). For BclI digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... using 500 ng of linearised plasmid that was retrieved from 5 μg of p-T3TS-nCas9n plasmid (plasmid #46757; Addgene, Cambridge, MA) digested with XbaI (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: Lhx6-Cre or Lhx6-Cre;Ppargc1aF/Fpups were injected at postnatal day (P)0 or P1 with 600nL of the following viral mix: AAV8-hSyn-dio-hM3D(Gq)-mCherry (Addgene #44361-AAV8) at a 1:40 dilution for sparse cell targeting ...
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bioRxiv - Cell Biology 2020Quote: ... SKO cells were transfected with pSH-EFIRES-P-GFP(1-10)opti AAVS1 Safe Harbor integration plasmid (Addgene plasmid # 129416, [35] and pCas9-sgAAVS1-2 (Addgene plasmid # 129727 [47]), and a stable pool was selected using puromycin (1 µg/ml puromycin for 6 days ...
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bioRxiv - Neuroscience 2021Quote: ... and SAD-B19 helper proteins (N, 52.11 mg, Addgene 59924; P, 30.15 mg, Addgene 59925; L, 23.70 mg, Addgene 59922; G, 20.26 mg, Addgene 59921). Cells were maintained with DMEM with GlutaMAX supplement ...
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bioRxiv - Microbiology 2023Quote: ... we transfected HEK 293T cells with a combination of a donor plasmid (p-GFP: MAP1LC3B-mEGFP gifted from Allen Institute for Cell Science - Addgene Ref 101783), a purified PCR product coding for the gRNA-tracrLC3B and a plasmid coding for Streptococcus pyogenes Cas9 (pCas9 ...
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bioRxiv - Cell Biology 2024Quote: ... The left and right homology arms for the AAVS1 locus and the puromycin gene were amplified individually from the pAAVS1-P-CAG-GFP plasmid (Addgene #80491; Oceguera-Yanez et al., 2016). These fragments were cloned into the pYTK089 backbone by Golden Gate using a standard protocol (Lee et al. ...