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Citations for Addgene :
201 - 250 of 961 citations for hsa mir 296 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
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bioRxiv - Cancer Biology 2023Quote: ... The bGH poly(A) signal was PCR amplified from pX459 (Addgene, 62988) using primers forward ...
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bioRxiv - Immunology 2023Quote: ... eGFP was amplified via PCR from pLJM-1-eGFP (Addgene: Plasmid #19319) using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... were ordered from GenScript (https://www.genscript.com) and PCR-cloned into pHIV-Luc-ZsGreen (Addgene plasmid #39196) using and EcoRI and XbaI digestion.
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bioRxiv - Neuroscience 2023Quote: ... and a synthetic intron was PCR-amplified from pDEST-APLO (Addgene 112805) (77) ...
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bioRxiv - Cell Biology 2023Quote: ... by PCR amplifying the ORFs from cDNA templates of HSC70 (19514, Addgene) and LAMP2A (86146 ...
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bioRxiv - Neuroscience 2023Quote: ... a Cas9-T2A fragment PCR amplified from pDEST-APIC-Cas9 (Addgene 121657) (9) ...
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bioRxiv - Cell Biology 2023Quote: ... with HA-TurboID amplified by PCR from 3xHA-TurboID-NLS_pCDNA3 (Addgene #107171) and inserted with a Gly-Gly-Gly-Ser linker either side of the HA-TurboID sequence ...
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bioRxiv - Systems Biology 2024Quote: ... CYC1t and pTEF1 were both PCR amplified from BB3cK_pGAP_23*_pTEF_Cas9 (Addgene #104909) (Gassler et al. ...
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bioRxiv - Systems Biology 2022Quote: ... and lambda phosphatase were purchased from Addgene (Addgene Kit #1000000094)7.
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bioRxiv - Cancer Biology 2021Quote: ... CyclinB1-GFP was amplified using PCR (donor plasmid: Addgene #26061, from Jonathon Pines) and was subcloned into pDONR221 and subsequently subcloned into pLX303 ...
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bioRxiv - Molecular Biology 2020Quote: ... ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425 ...
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bioRxiv - Genetics 2021Quote: ... The sequence of GeneSwitch was amplified by PCR using pElav-GeneSwitch (Addgene: 83957). The backbone vector pElav-GeneSwitch was digested with KpnI ...
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bioRxiv - Developmental Biology 2020Quote: MoxMaple3 was amplified by PCR from the pmCherry-T2A-moxMaple3 (Addgene Plasmid #120875) [43] and inserted into the pUASp by SpeI (3’)/EcoRI (3’) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons of sT and LT gene regions from plasmid RAZ1 (Addgene #114381) were used as target DNA templates (Refer Supplementary Table 1 for primers) ...
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bioRxiv - Genomics 2020Quote: ... PCR amplified and cloned into Esp3I-digested plentiGuide-Puro (Addgene plasmid ID: 52963) lentiviral vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... the natNT2 marker was PCR amplified from pUG-natNT2 (Addgene plasmid #110922, (26)) using primers 10297 and 10298 ...
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bioRxiv - Neuroscience 2019Quote: ... the iGluu gene was amplified by PCR from pCI-syn-iGluu (Addgene #106122) using Phusion polymerase (forward 5’-CATCAGGATCCATGGAGACAGACACACTCC-3’ ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
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bioRxiv - Bioengineering 2021Quote: ... Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966) as a template and cloned together with either pJ23119 or pAtU6 for sgRNA expression.
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bioRxiv - Cell Biology 2021Quote: The KIF18B coding sequence was amplified by PCR from 24xMoonTag-kif18b-24xPP7 (Addgene plasmid #128604 [38] was a gift from Marvin Tanenbaum ...
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bioRxiv - Neuroscience 2019Quote: ... nGFP was PCR amplified from pAd nGFP (Addgene, 19413, deposited by Douglas Melton) using primers (forward 5’-CATCATCGAT ATGGTGCACGTGGATCCAA −3’ ...
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bioRxiv - Biophysics 2022Quote: ... Kinesin 1 1-401 (K401) was PCR amplified from pWC2 plasmid (Addgene # 15960). Ncd 236-701 was PCR amplified from a plasmid gifted by Andrea Serra-Marques.
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bioRxiv - Microbiology 2020Quote: The SNAP-tag gene was PCR amplified from pSNAP-tag(m) (Addgene #101135) and cloned into pVpr-IN.eGFP(Albanese et al. ...
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bioRxiv - Microbiology 2021Quote: ... The Cas9 part was generated via PCR amplification of pCas9/Decoy (Addgene #80324)8 using primers P12/P13 ...
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bioRxiv - Molecular Biology 2019Quote: ... The ERT2 DNA fragment was PCR amplified from pCAG-Cre-ERT2 (Addgene #14797) using 1Fw and 1Rv primers and inserted between SalI and BamHI sites of pBudCE4.1-3xHA ...
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bioRxiv - Immunology 2020Quote: ... The mAID-Clover cassette was PCR amplified from the plasmid pMK290 (Addgene, #72828). These fragments together with a puro selection cassette amplified from pEN84 were assembled in an order diagramed in Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... secA5-YFP sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene), and subcloned into the pAAV-CAG-GFP (Addgene ...
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bioRxiv - Physiology 2021Quote: ... Fluorescent protein sequences were PCR-derived from pmVenus(L68V)-mTurquiose2 (Addgene plasmid #60493), Gamillus/pcDNA3 (Addgene plasmid #124837) ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24×_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Cancer Biology 2021Quote: Scarlet-H2A was amplified using PCR (donor plasmid: Addgene #85051, from Dorus Gadella) and subcloned into pDONR221 using the Gateway BP Clonase II enzyme mix (#11789020 ...
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bioRxiv - Neuroscience 2021Quote: ... the fragments were amplified with PCR and then assembled in pHD-DsRed (RRID:Addgene_51434) with NEBuilder (New England BioLabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... human MEG3 cDNA was PCR amplified from the pCI-Meg3 (Addgene Plasmid #44727) using NEB Q5 high-fidelity polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... and the ~350 bp PCR product was cloned directionally into pFRB-NLUC (Addgene) cut with the BamH1 and BsiW ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The nourseothricin resistance gene (NAT) was PCR-amplified from pICH47732:FCP:NAT (Addgene #85984) using primers 1 and 2 (Table S5) ...
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bioRxiv - Neuroscience 2022Quote: ... a GFP-kanamycin marker was PCR amplified from a template vector (PL452, Addgene). The primers include homology arms consisting of 50 nucleotides upstream and downstream from the start codon of the vav gene ...
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bioRxiv - Cancer Biology 2023Quote: ... Myristolated AKT was amplified by PCR from pT3-myr-AKT-HA (Addgene #31789) and cloned into the pSBbi-w/o-Puro backbone ...
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bioRxiv - Cell Biology 2023Quote: ... BirA was PCR amplified from pDisplay-BirA-ER (Alice Ting lab, Addgene #20856) with AttB sites as described above and transferred to pDEST12.2 or pLenti-DEST-EF1A-Hygro ...
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bioRxiv - Cell Biology 2023Quote: ... 10 pmol of PCR repair template amplified from pSNAP-tag plasmid (Addgene #101135) or mTagBFP2 plasmid (Addgene #75029 ...
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bioRxiv - Biophysics 2022Quote: ... plasmids vector was obtained from circular PCR amplification of pBLO62.5 (Addgene plasmid #123124) with two primers respectively pairing to N-terminal and C-terminal NLS sequence (Tsuchida et al. ...
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bioRxiv - Systems Biology 2023Quote: ... CSE1L and NUP37 were cloned by PCR into pEN_TT_miRc2 3xFLAG-BirA* (Addgene #192307). Full cloning details are available in Supplementary Data 7 ...
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bioRxiv - Cell Biology 2023Quote: ... the RhoA biosensor fragment was PCR amplified from the pLentiRhoA2G plasmid (Addgene #40179) to add BamHI and XhoI sites at the ends ...
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bioRxiv - Synthetic Biology 2023Quote: ... inverse PCR from CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) using a pair of primers including SacI restriction sites generated a linear plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... were amplified by PCR and cloned into pLenti-GFP-puro (Addgene plasmids #17481) Lentivirus were produced by transforming pLenti constructs ...
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bioRxiv - Synthetic Biology 2023Quote: ... The first fragment was PCR amplified from pETM6-HCAmp-EV (Addgene Cat#49795)(P ...
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bioRxiv - Bioengineering 2023Quote: ... difficle genome by PCR and cloned into the 2Bc-T plasmid (Addgene #37236); Nluc ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... and the p10-3’UTR PCR amplified from the plasmid pJFRC81 (Addgene #36432). These were then cloned by Gibson assembly (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Templates for sgRNA synthesis were generated by PCR from a pX330 template (Addgene), sgRNAs were transcribed in vitro and purified (Megashortscript ...
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bioRxiv - Neuroscience 2024Quote: ... and ii) PCR fragment of FLPo originated from pTCAV-FLEx-FLPo (#67829, Addgene). Because a nuclear localization signal (NLS ...
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bioRxiv - Molecular Biology 2024Quote: ... the coding sequences of FLPo were PCR-amplified from pQUAST-FLPo (#24357, Addgene) and ligated into the XhoI-digested pUASz1.0 vector [45] using In-Fusion ...