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Citations for Addgene :
551 - 600 of 980 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... with flanking sequences to enable PCR amplification and Gibson assembly into lentiGuide-Puro (pLG, Addgene #52963). The pooled oligo library was amplified using pLG_U6_foward 5’- GGCTTTATATATCTTGTGGAAAGGACGAAACACCG-3’ and pLG-TRACR_reverse 5’-GACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’ ...
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bioRxiv - Cell Biology 2023Quote: Mouse HA-Sox9-P2A and P2A-Cre blocks were PCR-amplified from pWPXL-Sox9 (Addgene, #36979) and AAV:ITR-U6-sgRNA(backbone)-TBG-Cre-WPRE-hGHpA-ITR(Katsuda et al. ...
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bioRxiv - Cell Biology 2023Quote: Mouse HA-Sox4-P2A and P2A-Cre blocks were PCR-amplified from pLVXT-Sox4 (Addgene, #101121) and AAV:ITR-U6-sgRNA(backbone)-TBG-Cre-WPRE-hGHpA-ITR31 respectively ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was digested with KpnI and XhoI and integrated into pSH1.6EGFP (Plasmid #42323, Addgene). The Flot1 gene was amplified from genomic DNA from YT16 rice using the primers HindIII_OsFlot1Prom1-F1 and Eco47III_OsFlot-R1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-HTR2A was generated by PCR amplifying the HTR2A CDS from HTR2A-Tango (Addgene #66409) (introducing a stop codon ...
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bioRxiv - Synthetic Biology 2023Quote: ... pcDNA3.1_Signal-Flag-ADRB2 was generated by PCR amplifying the ADRB2 CDS from ADRB2-Tango (Addgene #66220) (introducing a stop codon ...
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bioRxiv - Cell Biology 2023Quote: Keratin sequences were cloned by polymerase chain reaction (PCR) from pBabe-RFP1-KRT5-hygro (Addgene #58493) and pDONR22-KRT6A (DNASU clone HsCD00039474 ...
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bioRxiv - Cell Biology 2023Quote: ... Both pie-1p and pie-1 3’UTR PCR products were amplified using pPK605 plasmid (Addgene) as the template.
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A quantitative characterization of early neuron generation in the developing zebrafish telencephalonbioRxiv - Developmental Biology 2023Quote: ... pCS2-Cre-ERT2 was generated by inserting PCR-generated ERT2 sequence (with pBigTB-CreERT2 (Addgene #149434) as template ...
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bioRxiv - Neuroscience 2023Quote: ... oligonucleotide pairs (Extended Data Table 4) were used for PCR and inserted into pCFD5 (Addgene #73914) via Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: ... The NFAST sequence was amplified by PCR from the Addgene plasmid pAG148 FRB-NFAST (Addgene #130812) and ligated at the 3’ of ER-targeting sequence into pcDNA3.
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bioRxiv - Synthetic Biology 2023Quote: ... Human FUS (residues 1-214) was amplified by PCR using pHR-FUSN-mCh-Cry2WT (Addgene #101223). The IDRs fragments were fused with TPPPCORE domain(aa.45-166 ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length and domain of interest were amplified by PCR using emGFP-BAZ1B (#65372; Addgene) as a template and inserted into pEGFP-C2 vector (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: The VAPB-Wt expression vector was produced through PCR amplification using mCherry-VAPB (Addgene plasmid – 108126) as the template and the following primers ...
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bioRxiv - Physiology 2023Quote: ... The PCR product was cloned via AgeI/BamHI into an MCS-BioID2-HA plasmid (Addgene #74224)15 to enable expression of an NCLX-BioID2-HA fusion protein under the control of a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... were cloned by PCR as EcoRI–BamHI fragments into the vector pcDNA3.1 mycBioID52 (Addgene plasmid #35700) in frame with BirA ...
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bioRxiv - Molecular Biology 2023Quote: ... the MCP coding sequence was PCR amplified from UBC NLS-HA-2XMCP-tagRFPt vector (Addgene 64541) using MCP F and MCP R primers and inserted in-frame ...
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bioRxiv - Developmental Biology 2023Quote: ... flanked by Xba1 sites was generated by touch down PCR using pUAST-Rab35-6myc (Addgene #53503) as template.
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was further digested using BamHI along with tol2kit 101_p5E-Ubiquitin plasmid (Addgene #27320). Both were purified and 101_p5E-Ubiquitin was dephosphorylated using NEB Antarctic phosphatase following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was PCR-amplified from wt-TDP43- tdTomato-HA plasmid (Addgene 28205, a gift from Zuoshang Xu)63 and cloned in frame with the N-terminal 6- His-SUMO-TEV-site into the SspI site of pET 6His-SUMO-TEV LIC (1S ...
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bioRxiv - Immunology 2024Quote: ... The OVA entry fragment was created using a PCR amplicon from OVA plasmid (Addgene plasmid 64599) with primers containing attB sites (Table 1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli codon optimized dSpyCas9 was PCR amplified from vector pdCas9 (gift from Luciano Marraffini, Addgene plasmid #46569) (43 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then cloned into vector pPD95.77 (Fire Lab C. elegans vector kit; Addgene, Cambridge, MA) via the SalI and BamHI sites ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PCR product was subsequently cloned in the E1b-GFP-Tol2-Gateway construct (Addgene #37846; (71) by Gateway cloning ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR product was cloned into the BGlII/EcoR1 sites of the AAV-hSyn-EGFP (Addgene, 114213), upstream of the human Synapsin 1 promoter ...
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bioRxiv - Genomics 2020Quote: ... The ORI minimal promoter and flanking region was PCR amplified from the hSTARR-seq plasmid (Addgene #99296) with a custom primer that included homology for Gibson cloning and KAPA HiFi HotStart ReadyMix (Kapa KK2602 ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Neuroscience 2021Quote: ... The GFP coding region containing artificial introns was PCR-amplified from the vector pPD95.69 (Addgene plasmid #1491) using primers to add segments encoding SGGGGS and SGGGTS to flank the N- and C-termini of GFP ...
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bioRxiv - Cancer Biology 2021Quote: ... the tetracycline inducible expression construct was PCR-amplified from pCW57.1 (a gift from David Root, Addgene # 41393) and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs ...
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bioRxiv - Cell Biology 2021Quote: ... a wild copy of the TYR gene was PCR amplified form the pEGFP-TYR plasmid (Addgene, 32781) with NheI and AgeI overhangs ...
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bioRxiv - Cell Biology 2021Quote: ... were amplified with PCR and cloned into Ac5-Stable2-neo (Rosa Barrio and James Sutherland60, Addgene 32426) with inserts removed by PCR.
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel-purified and cloned into EcoRV-cut pLenti CMV Puro DEST (w118-1) (Addgene #17452 ...
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bioRxiv - Cell Biology 2021Quote: ... The miRFP670 was amplified by PCR from pmiRFP670-N1 (a gift from Vladislav Verkhusha, Addgene plasmid #79987) (Shcherbakova et al. ...
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bioRxiv - Genetics 2022Quote: ... as the selection module for all TFT constructs and obtained this sequence by PCR amplification from Addgene plasmid #41030 (a gift from Michael Boddy) ...
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bioRxiv - Cancer Biology 2022Quote: ... After PCR the insert was gel purified (GeneJet) and Gibson cloned into BsmBI-digested pXPR_053 (Addgene# 113591). Gibson cloned insert and vector was column purified (GeneJet ...
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bioRxiv - Bioengineering 2022Quote: ... another fragment coding sfGFP was PCR-amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (#60904, Addgene) using oKK334 and oKK335 ...
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bioRxiv - Developmental Biology 2022Quote: ... EF1alpha-GCamp6m lentiviral vector was generated by PCR amplification of GCamp6m from pGP-CMV-GCaMP6m (Addgene #40754) using with Q5 High Fidelity master mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... the doxycycline inducible Tet-On-3G_hPGK promoter sequence was PCR amplified from the plasmid pMK243 (Addgene, #72835) with 5’ SalI and 3’ MluI digestion sites ...
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bioRxiv - Cell Biology 2022Quote: ... The BG4 sequence was amplified by PCR from the pSANG10-3F-BG4 plasmid (Addgene plasmid # 55756(12)) while the mitochondrial targeting sequence (MTS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We PCR amplified LexApr using oligos 1195 and 1240 from FRP1642 (Addgene #58442, Ottoz et al. 2014). We PCR amplified CYC1 terminator from pSZB1040 using oligos 2194 and 2195 ...
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bioRxiv - Microbiology 2019Quote: ... NSP5 and NSP2-mCherry were amplified by PCR and inserted into the plasmid pAIP (Addgene #74171; (30) at the NotI-EcoRI restriction enzymes sites to yield pAIP-NSP5 and pAIP-NSP2-mCherry ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared by PCR and transferred to a retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
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bioRxiv - Neuroscience 2021Quote: ... The low affinity glutamate sensor iGluSnFR S72A was PCR amplified from the pAAV.hSynapsin.SF-iGluSnFR.S72A which was a gift from Loren Looger (Addgene plasmid # 106176 ...
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bioRxiv - Developmental Biology 2020Quote: 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019). Guide RNAs and the donor vector were co-injected into vas-Cas9 embryos (BDSC #51324 ...
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bioRxiv - Microbiology 2019Quote: ... we PCR-amplified the G418 resistance cassette from the plasmid pFA6a-6xGLY-Myc-kanMX6 (Addgene, plasmid #:20769), flanked by 50-bp upstream and downstream regions of the start and stop codons of the corresponding heme biosynthetic gene ...
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bioRxiv - Molecular Biology 2021Quote: ... 2500 ng of purified HDR PCR product and 1500 ng of guide RNA expression plasmid (Addgene 49330) containing the relevant guide RNAs (Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2022Quote: ... was used for PCR reactions with 30-50 ng of pScarlessHD-DsRed plasmid as template (Addgene #64703) or 50 ng of gBlocks or genomic DNA from the same Drosophila strain (BDSC #55821) ...
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bioRxiv - Biochemistry 2022Quote: ... Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386 ...
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bioRxiv - Genetics 2022Quote: ... 1 kb homology arms were generated through PCR and cloned into the pHD-dsRed-attP (Addgene #51019) [73] ...
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bioRxiv - Synthetic Biology 2022Quote: ... It was synthesised by PCR amplifying the αGCN4 backbone from LLP252 pEF1a-NLS-scFvGCN4-DNMT3a (Addgene, #100941) and the first 639 bp of the 5’ end of p65HSF1 from pAC1393-pmax-NLSPUFa_p65HSF1 (Addgene ...