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Citations for Addgene :
151 - 200 of 1613 citations for TRAILR 2 TNFRSF10B Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Retrovirus were produced by transfecting HEK 293T cells with a mixture of PD-L1-pMIT1.1 (or PD-L2-pMIT1.1) and pCL-Eco (Addgene #12371) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T cells were transfected with the respective genome library along with helper plasmids pCAG-B19N (Addgene cat. # 59924), pCAG-B19P (Addgene cat ...
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bioRxiv - Cancer Biology 2024Quote: ... #196709)52 and packaged into lentivirus in HEK-293T (ATCC) using plasmids psPAX2 (a gift from Didier Trono, Addgene, #12260) and pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Developmental Biology 2022Quote: ... The empty ctrl or MMP14-eGFP lentiviral plasmid (7.5 μg) was co-transfected into 293 cells with packaging plasmid pRSV-Rev (5 μg, Addgene #12253), pMDLg/pRRE (2.5 μg ...
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bioRxiv - Immunology 2022Quote: ... or the scrambled shRNA sequence: CCGGCA-ACAAGATGAAGAGCACCAATTTTT were transfected overnight into 293 LentiX packaging cells (Takoro) along with VSV-G plasmid envelope (Addgene), aiming to generate shLRBA and shControl encoding lentiviruses ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 293/hACE2 stable cells were generated by transduction of 293 cells using lentivirus produced in 293T cells utilizing plasmids: pCMV-dR8.2-dvpr (Addgene #8455); pCAGGS-G-Kan (Kerafast EH1017) ...
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bioRxiv - Microbiology 2023Quote: ... in 293-LTV cells transfected using jetPRIME (Polypus 101000027) with plasmids pCMV-VSV-G (a gift from Bob Weinberg Addgene plasmid # 8454 ...
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bioRxiv - Cell Biology 2023Quote: Replication-deficient lentiviral particles were produced by CaCl2-transfection of 293-T cells with the packaging vector psPAX2 (Addgene, #12260), the envelope vector pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... pAAV_hsyn_NES-his-CAMPARI2-F391W-WPRE-SV40 was a gift from Eric Schreiter (Addgene plasmid # 101061)31 ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: A plasmid encoding obligate dimeric TGT was cloned from the TGT-His plasmid (Addgene #138201) using DNA HiFi Assembly (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... A1AT/ SERPINA1 and PAI1/ SERPINE1 cDNAs were amplified from SERPINA-bio-His plasmid (Addgene #52182) and SERPINE1-bio-his (Addgene #52077 ...
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bioRxiv - Molecular Biology 2020Quote: ... strains of interest were transformed with a plasmid containing His-tagged SUMO (Smt3-Hisx7) (Addgene) under the control of a copper inducible promoter ...
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bioRxiv - Cell Biology 2021Quote: ... from the pcDNA3.1 Flag-His-ATM plasmid (kind gift of Michael Kastan; Addgene plasmid #31985) (67 ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid pET28HIS-hAPE1 (for WT His-APE1 protein) was a gift from Primo Schaer (Addgene plasmid #70757 ...
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bioRxiv - Pathology 2024Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
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bioRxiv - Neuroscience 2023Quote: ... and the injections of AAV5-hSyn-NES-his-CaMPARI2-WPRE-SV40 (2.5x10^12gc/ml Addgene 200nl measured using a Nano Injector ...
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bioRxiv - Biophysics 2019Quote: Genetic constructs encoding the inward rectifying potassium channel Kir2.1 and the blue-shifted channelrhodopsin CheRiff were separately cloned into lentiviral expression backbones (FCK-CMV) and then co-expressed in HEK 293T cells along with the lentiviral packaging plasmid PsPAX2 (Addgene) and the envelope plasmid VsVg (Addgene ...
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bioRxiv - Bioengineering 2020Quote: ... 400,000 HEK cells were seeded in 6-well plates and the next day transfected with 1 μg psPAX2 (Addgene #12260), 3 μg pCMV-VSV.G (Addgene ...
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bioRxiv - Neuroscience 2022Quote: For HEK-cell expression, mutations were introduced in previously described ACR constructs of GtACR1 (Wietek et al., 2016) (Addgene #85464) and PhobosCA (Wietek et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... we transfected HEK 293T/17 cells with different plasmids together with the packaging plasmids pMD2.G (Gift from Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Microbiology 2021Quote: ... HEK 293T cells in 10cm dishes were transfected at 80% confluency with the lentiviral plasmid pLenti-CMV-Puro (Addgene #17452) containing the gene of interest (2 μg) ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... HEK 293T cells were transfected with lentiviral vectors and packaging vectors (pCMV-VSVG and psPAX2, Addgene # 8454 and 12260, respectively), using the Mirus TransIT-LT1 transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S-Pseudotyped lentivirus27,43 were produced by co-transfection of HEK-293T cells with plasmids bearing a GFP reporter gene (pLB was a gift from Stephan Kissler, Addgene plasmid #11619 ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral constructs were also packaged in HEK-293T cells by transient co-transfection with the packaging constructs psPax2 (Addgene #12260) and pMΔ2.G (Addgene #12259) ...
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bioRxiv - Physiology 2022Quote: ... 60% confluent monolayers of 293-T cells were transfected with LV shuttle vector pUltra-hot-LT and the packaging plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...
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bioRxiv - Biophysics 2022Quote: ... Lentivirus expressing LifeAct-GFP were produced in HEK 293T cells by cotransfecting the lentiviral plasmids pLenti.PGK.LifeAct-GFP.W (a gift from Rusty Lansford, Addgene plasmid #51010; Watertown, MA) with psPAX2 and pMD2 ...
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bioRxiv - Immunology 2021Quote: TXN1-/- HEK 293T cells were prepared by transient transfection of HEK 293T cells stably expressing Cas9 with sgRNA for TXN1 packaged in lentiGuide-Puro (Addgene, 52963) using the following oligo sequences (5’-ACGTGATATTCCTTGAAGTA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviruses were produced by transfecting HEK-293T cells with the transfer lentiCRISPR v2 plasmids and packaging plasmids pLTR-G (Addgene, 17532) and pCD/NL-BH*DDD (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... HEK 293T cells (ATCC CRL-11268) were transfected with expression vectors for the ribozyme-flanked viral genome (cSPBN-4GFP (Addgene 52487) or pRVΔG-4tdTomato (Addgene 52500)) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus constructs were also packaged in HEK-293T cells by transient transfection of the constructs in combination with the packaging constructs psPax2 (Addgene #12260) and pMΔ2.G (Addgene #12259) ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNA gene knockdown or gene overexpression lentiviral vectors were transfected into HEK 293FT cells together with a packaging plasmid (psPAX2; Addgene; #12260) and envelope plasmid (pMD2G ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses were produced by triple transfection of HEK-293T cells with the lentiviral transfer vector pLenti6.3/TO/V5-Blasti and the packaging plasmids psPAX2 (Addgene plasmid #12260) and pMD2.G (Addgene plasmid #12259) ...
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bioRxiv - Cell Biology 2022Quote: ... replication-deficient lentiviruses were produced and titrated as described by co-transfection of the resulting constructs in HEK-293T cells with the HIV-1 packaging plasmid psPAX2 (Addgene #12260) and the plasmid pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2023Quote: ... viruses were produced in HEK 293T cells by transfecting the corresponding plasmid o interest as well as with pVSVg (Addgene, #8454) and psPAX2 (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviral supernatants were generated by transfecting one 10-cm plate of HEK-293T cells at 90% confluency with 3 µg pMD2.G (Addgene #12259), 9 µg psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was generated by transfection of HEK-293T cells with lentiCRISPRv2 (sgC15, sgC40 or sgCtrl) and the packaging plasmids psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... pEZYflag and pEZYmyc-His were a gift from Yu-Zhu Zhang (Addgene plasmids #18700 and #18701). Gateway destination vectors for BiFC for N- and C-terminal tagging with Venus fluorescent protein fragments (pEZY BiFC N NV ...
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bioRxiv - Biochemistry 2021Quote: ... The His-Sumo bacterial version was codon optimized and cloned into K27-Sumo (Addgene ID 169193) via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... His-Strep2-tag from 438-SNAP-V3 vector a gift from Scott Gradia (Addgene plasmid # 55223) with inserting CATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCAT sequence right in front of Strep II gene by PCR primer ...
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bioRxiv - Biochemistry 2020Quote: MBP-hnRNPA2 LC, soluble His-tag purification as described (Ryan et al., 2018) (Addgene ID: 98661)
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272 ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... coli and subcloned in-line with his-tagged MBP construct (2CT-10 vector, Addgene plasmid #55209). Recombinant MBP-EndoH fusion was expressed in and purified from E ...
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bioRxiv - Cancer Biology 2023Quote: SULF2 ORF including C-terminal Myc-His tag was subcloned from its source vector (Addgene # 13003) to lentiviral transfer vector pHR-CMV-TetO2_3C-Twin-Strep (Addgene # 113883 ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-human β-catenin (Addgene #24193), and NLS-mCherry (Addgene #49313 ...
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bioRxiv - Molecular Biology 2022Quote: ... pscALPSpuro-HsACE2 (human) (Addgene, MA, USA) were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... human a-SYN (A53T mutant) (Addgene), and mt-Keima (Addgene) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Cancer Biology 2024Quote: ORF encoding human MARK2 (Addgene, 23404) was cloned into pFL system with an N-terminal Strep2SUMO tag ...