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Citations for Addgene :
51 - 100 of 1583 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the coding sequence of the extracellular region of human TfR1 (residues 89–760) were amplified by PCR and inserted into pCMV6-XL4 FLAG-NGRN-Fc (Addgene #115773) with EcoRV and XbaI sites ...
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bioRxiv - Bioengineering 2019Quote: Images of HEK cells co-expressing hGlut1 (Addgene #18085) and iGlucoSnFR.mRuby2 were acquired on an inverted Zeiss LSM 800 confocal microscope equipped with GaAsP detectors and an EC Plan-Neofluar 10x/0.30 M27 objective ...
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bioRxiv - Cancer Biology 2020Quote: ... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and SERPINE1-bio-his (Addgene #52077) which were gifts from Gavin Wright without signal sequence to prevent cleavage of fused proteins (75) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139113)
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bioRxiv - Developmental Biology 2021Quote: pET-28b-RfxCas13d-His (Addgene #141322) plasmid containing the T7 promoter was linearized by using NotI restriction site ...
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bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-BMAL1-His (AddGene CN: 31367), pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN ...
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bioRxiv - Developmental Biology 2023Quote: pcDNA3-BMAL1-His (AddGene CN: 31367) were purchased from the Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... and IgG2a Fc (Addgene #114492), were amplified and extracted similarly ...
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bioRxiv - Immunology 2021Quote: ... HEK cells were transfected with pMD2.G (Plasmid 12259; Addgene); psPAX2 (Plasmid 12260 ...
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bioRxiv - Cell Biology 2024Quote: ... or human Mon1b (5’-GATGTGCAGATGGAGGTCGG-3’) were cloned into pSpCas9(BB)-2A-Puro (PX459) (Addgene #48139). The donor constructs used for homologous recombination were generated by cloning into the pUC19 vector with two ∼600-800-nucleotide fragments of genomic DNA upstream and downstream of the start codon of human USP8 ...
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bioRxiv - Biochemistry 2024Quote: ... human PLD3 sgRNA (5′-3′) was cloned into pSpCa9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) as described (Ran et al. ...
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bioRxiv - Immunology 2019Quote: ... and 6-His tag (Addgene plasmid# 50803) [36] ...
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bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139112)
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bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139116)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139117)
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bioRxiv - Cancer Biology 2020Quote: Lentiviruses were packaged in HEK 293T cells using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentiviruses were generated by transfecting HEK-293T cells with pCMVR8.74 (RRID:Addgene_22036), pMD2G (RRID:Addgene_12259) ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus was produced in HEK 293T using psPAX2 (Addgene plasmid # 12260) and VSV-G (Addgene plasmid # 12259 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
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bioRxiv - Molecular Biology 2019Quote: ... MmEsco2-myc/his and H2B-mCherry were amplified from the vectors pcDNA3.1/myc-His and pcDNA3-H2B-mCherry (Addgene, 20972), respectively ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The CyPet gene was amplified by PCR from the pCyPet-His vector (pCyPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14030 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The YPet gene was amplified by PCR from the pYPet-His vector (pYPet-His was a gift from Patrick Daugherty; Addgene plasmid # 14031 ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139124, 139125 respectively)
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bioRxiv - Biochemistry 2021Quote: ... pFastBac1 His-MBP (#30116) were procured from Addgene. pNIC28-MBP was constructed by replacement of TrxT with MBP [22] ...
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bioRxiv - Bioengineering 2022Quote: ... the plasmid pET-28b-Cas9-His (Addgene #47327) was transformed into Rosetta (DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... and transfected into HEK-293T cells together with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were transfected with pRK5-TGFβRI-FLAG plasmid (Addgene, #14833) using Lipofectamine 3000 transfection kit ...
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bioRxiv - Microbiology 2023Quote: ... HEK 293Ts were reverse-transfected with pcDNA3-AKT-PH-GFP (Addgene #18836) or pcDNA3-AKT-PH[R25C]-GFP (Addgene #18837) ...
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bioRxiv - Bioengineering 2024Quote: ... and then HEKs were transfected with envelope plasmid pMD2.G (Addgene #12259), packaging plasmids pMDLG/pRRE (Addgene #12251 ...
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bioRxiv - Developmental Biology 2019Quote: The GLI-3 bs-2 plasmid carrying the human GLI3 (Kinzler et al., 1988) was purchased from Addgene. F387F*fs mutation was generated in the vector pCDNA3 hGli3 using Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... and then transfected into HEK-293T cells together with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Biochemistry 2021Quote: ... was packaged in HEK 293T cells using helper plasmids pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Cell Biology 2019Quote: ... Lentiviruses were generated in HEK 293T cells transfected with psPAX2 (Addgene plasmid 12260), pCMV-VSV-G (Addgene plasmid 8454 ...
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bioRxiv - Microbiology 2023Quote: ... was transfected into HEK-293T cells along with packaging plasmids pΔ8.74 (Addgene, 22036) and pMD2G (Addgene ...
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bioRxiv - Developmental Biology 2021Quote: ... the pET-28b-RfxCas13d-His vector (Addgene, Plasmid #141322) was used for RfxCas13d protein production (Bon Opus Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... The FLAG-His sequence in payload plasmid pKM491 (Addgene) was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139126, 139127, 139128)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139114, 139115 respectively)
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bioRxiv - Cell Biology 2023Quote: ... pET-His-GST-tev-LIC (Addgene #29655, Gradia Lab), pGEX-4T-3-mR7BD (Addgene #79149 ...
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bioRxiv - Genetics 2023Quote: ... individual single and 3-plex crRNA constructs were cloned into the human U6 promoter-driven expression vector pRG212 (Addgene 149722 ...
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bioRxiv - Molecular Biology 2019Quote: Mouse SEMA6A-Fc and SEMA6c-Fc expression constructs were a gift from Woj Wojtowicz (Addgene plasmids 72163 and 72167, respectively) and human SEMA6A was gene-synthesized (GeneArt) ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse Sema6a-Fc and Sema6c-Fc expression constructs were a gift from Woj Wojtowicz (Addgene plasmids 72163 and 72167, respectively). Plasmid pHis1522 encoding his-tagged TcsL was synthesized and codon optimized for Bacillus megaterium (Genscript) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... United States).62,63 Plasmid constructs pcDNA3-sACE2(WT)-Fc(IgG1) and pcDNA3-sACE2-T92Q-Fc(IgG1) were obtained from Addgene (United States). The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK/TLR4 cells were transiently transfected with pcDNA3.1(-)hACE2 (Addgene plasmid #1786) to generate HEK/ACE2 or HEK/TLR4/ACE2 cell lines ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral particles were produced in HEK-293T cells with the vectors psPAX2 (12259, Addgene), pMD2.G (12259 ...
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bioRxiv - Genetics 2022Quote: ... HEK- 293T cells were transfected with 100 ng pIS0 (Addgene #12178, encoding firefly luciferase), 100 ng of the Renilla reporter and 800 ng of empty vector (1 µg total plasmid DNA ...
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bioRxiv - Neuroscience 2019Quote: ... Lentivirus were generated using co-transfection into HEK-293t cells of psPAX2 (Addgene, 12260), pMD2.G (AddGene ...