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Citations for Addgene :
101 - 150 of 453 citations for TFF3 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Bioengineering 2020Quote: ... A ChR2-expressing Spiking HEK cell line was created by Lipofectamine transfection with pcDNA3.1/hChR2(H134R)-EYFP (Addgene, Watertown ...
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bioRxiv - Synthetic Biology 2020Quote: Lentivirus production was performed using HEK-293FT cells and second-generation helper plasmids MD2.G (Addgene plasmid #12259) and psPax2 (Addgene plasmid #12260) ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviruses were produced in HEK 293T cells by cotransfection of lentiviral constructs with third generation packaging plasmids (Addgene) for 48–72 hr ...
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/-HEK 293T cells were generated using the CRISPR/Cas9 system from Addgene (http://www.addgene.org/). The sequences for guide RNAs were obtained from http://tools.genome-engineering.org and https://www.addgene.org/pooled-library/zhang-human-gecko-v2/ ...
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bioRxiv - Cell Biology 2023Quote: ... in HEK 293T cells (ATCC, VA) using 3rd generation lentiviral packaging plasmids pMDLg/pRRE (Addgene Inc., Plasmid 12251) and pRSV-RV (Addgene Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells seeded into 12 well plates were transfected with the retroviral packaging plasmid pUMVC (Addgene: 8449), pLXIN (Clontech ...
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bioRxiv - Cancer Biology 2021Quote: ... The 293 cells were seeded in 10-cm-diameter dishes and transfected with pCMV-dR8.2 dvpr (Addgene plasmid #8455), pCMV-VSV-G (Addgene plasmid #8454 ...
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bioRxiv - Cell Biology 2020Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol (Tropea et al. ...
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bioRxiv - Bioengineering 2019Quote: ... TGFB1-bio-His (proTGFβ) which was a gift from Gavin Wright (Addgene plasmid # 52185) [17] and HA-OVOL2 (OVOL2 ...
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bioRxiv - Cell Biology 2019Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol(Tropea et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
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bioRxiv - Biochemistry 2022Quote: ... pET29b-IPP1-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124137). 15µL of Lemo21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... coli MG1655 into a His-SUMO-TEV (HST) plasmid backbone (Addgene product number: 48313). Each HST construct was grown in LB to an OD600 of 0.4 at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
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bioRxiv - Biophysics 2019Quote: ... virus was produced in HEK 293T cells by co-transfecting the lentiviral plasmids pLenti.PGK.LifeAct-Ruby.W (a gift from Rusty Lansford - Addgene plasmid #51009) with psPAX2 and pMD2.G (a gift from Didier Trono - Addgene plasmid #12260 and #12259) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Lentiviral particles were produced in 293T HEK cells by co-transfection of the lentiviral pCW57.1_Gata6 plasmid with VSV-G (pMD2.G, Addgene #12259) and helper plasmid (psPAX2 ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus were produced by transfecting HEK 293T cells with a mixture of PD-L1-pMIT1.1 (or PD-L2-pMIT1.1) and pCL-Eco (Addgene #12371) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-293T cells were transfected with the respective genome library along with helper plasmids pCAG-B19N (Addgene cat. # 59924), pCAG-B19P (Addgene cat ...
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bioRxiv - Cancer Biology 2024Quote: ... #196709)52 and packaged into lentivirus in HEK-293T (ATCC) using plasmids psPAX2 (a gift from Didier Trono, Addgene, #12260) and pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Developmental Biology 2022Quote: ... The empty ctrl or MMP14-eGFP lentiviral plasmid (7.5 μg) was co-transfected into 293 cells with packaging plasmid pRSV-Rev (5 μg, Addgene #12253), pMDLg/pRRE (2.5 μg ...
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bioRxiv - Immunology 2022Quote: ... or the scrambled shRNA sequence: CCGGCA-ACAAGATGAAGAGCACCAATTTTT were transfected overnight into 293 LentiX packaging cells (Takoro) along with VSV-G plasmid envelope (Addgene), aiming to generate shLRBA and shControl encoding lentiviruses ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 293/hACE2 stable cells were generated by transduction of 293 cells using lentivirus produced in 293T cells utilizing plasmids: pCMV-dR8.2-dvpr (Addgene #8455); pCAGGS-G-Kan (Kerafast EH1017) ...
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bioRxiv - Microbiology 2023Quote: ... in 293-LTV cells transfected using jetPRIME (Polypus 101000027) with plasmids pCMV-VSV-G (a gift from Bob Weinberg Addgene plasmid # 8454 ...
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bioRxiv - Cell Biology 2023Quote: Replication-deficient lentiviral particles were produced by CaCl2-transfection of 293-T cells with the packaging vector psPAX2 (Addgene, #12260), the envelope vector pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... pAAV_hsyn_NES-his-CAMPARI2-F391W-WPRE-SV40 was a gift from Eric Schreiter (Addgene plasmid # 101061)31 ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Bioengineering 2021Quote: ... We created 2 bacterial expression vectors: pET28-His-MBP-NLS-RfxCas13d-NLS-HA (Addgene 171586) produces the protein RfxCas13d-sTag after cleavage of the N-terminal His-MBP fusion partner ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: A plasmid encoding obligate dimeric TGT was cloned from the TGT-His plasmid (Addgene #138201) using DNA HiFi Assembly (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... A1AT/ SERPINA1 and PAI1/ SERPINE1 cDNAs were amplified from SERPINA-bio-His plasmid (Addgene #52182) and SERPINE1-bio-his (Addgene #52077 ...
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bioRxiv - Molecular Biology 2020Quote: ... strains of interest were transformed with a plasmid containing His-tagged SUMO (Smt3-Hisx7) (Addgene) under the control of a copper inducible promoter ...
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bioRxiv - Cell Biology 2021Quote: ... from the pcDNA3.1 Flag-His-ATM plasmid (kind gift of Michael Kastan; Addgene plasmid #31985) (67 ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid pET28HIS-hAPE1 (for WT His-APE1 protein) was a gift from Primo Schaer (Addgene plasmid #70757 ...
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bioRxiv - Pathology 2024Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
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bioRxiv - Neuroscience 2023Quote: ... and the injections of AAV5-hSyn-NES-his-CaMPARI2-WPRE-SV40 (2.5x10^12gc/ml Addgene 200nl measured using a Nano Injector ...
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bioRxiv - Biophysics 2019Quote: Genetic constructs encoding the inward rectifying potassium channel Kir2.1 and the blue-shifted channelrhodopsin CheRiff were separately cloned into lentiviral expression backbones (FCK-CMV) and then co-expressed in HEK 293T cells along with the lentiviral packaging plasmid PsPAX2 (Addgene) and the envelope plasmid VsVg (Addgene ...
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bioRxiv - Bioengineering 2020Quote: ... 400,000 HEK cells were seeded in 6-well plates and the next day transfected with 1 μg psPAX2 (Addgene #12260), 3 μg pCMV-VSV.G (Addgene ...
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bioRxiv - Neuroscience 2022Quote: For HEK-cell expression, mutations were introduced in previously described ACR constructs of GtACR1 (Wietek et al., 2016) (Addgene #85464) and PhobosCA (Wietek et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... we transfected HEK 293T/17 cells with different plasmids together with the packaging plasmids pMD2.G (Gift from Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Microbiology 2021Quote: ... HEK 293T cells in 10cm dishes were transfected at 80% confluency with the lentiviral plasmid pLenti-CMV-Puro (Addgene #17452) containing the gene of interest (2 μg) ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... HEK 293T cells were transfected with lentiviral vectors and packaging vectors (pCMV-VSVG and psPAX2, Addgene # 8454 and 12260, respectively), using the Mirus TransIT-LT1 transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S-Pseudotyped lentivirus27,43 were produced by co-transfection of HEK-293T cells with plasmids bearing a GFP reporter gene (pLB was a gift from Stephan Kissler, Addgene plasmid #11619 ...
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bioRxiv - Cancer Biology 2021Quote: Lentiviral constructs were also packaged in HEK-293T cells by transient co-transfection with the packaging constructs psPax2 (Addgene #12260) and pMΔ2.G (Addgene #12259) ...
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bioRxiv - Biochemistry 2023Quote: ... Lentiviruses with this construct were produced by transfecting human embryonic kidney 293T (HEK 293T) cells with pKS18 and the lentiviral packaging vectors pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Physiology 2022Quote: ... 60% confluent monolayers of 293-T cells were transfected with LV shuttle vector pUltra-hot-LT and the packaging plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene ...