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Citations for Addgene :
351 - 400 of 1843 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
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bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293/T17 cells were transfected with DNAJB1-PRKACA K128H plasmid along with psPAX2 (Addgene plasmid #12260, gift from Didier Trono) and pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: Rhodopsin protein was expressed in HEK293 cells using transient transfection (pcDNA3 rod opsin construct, a gift from Robert Lucas (Addgene plasmid # 109361 ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected in 96-well plates with the 8xGTIIC-luciferase plasmid (firefly luciferase, # 34615, Addgene, Watertown, MA, US) and the pRL-SVl40P plasmid (Renilla luciferase ...
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bioRxiv - Cell Biology 2022Quote: ... subcloning from the following constructs: Flag-Axin1 purchased from Addgene (#109370), tdmRuby3 from Max Wilson into XLone-GFP purchased from Addgene (#96930 ...
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bioRxiv - Biochemistry 2019Quote: ... and FLAG-eIF4E2 was a gift from Dong-Er Zhang (Addgene plasmid # 17342 ...
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bioRxiv - Cancer Biology 2020Quote: ... pBABE-FLAG-LKB1 WT were from Lewis Cantley (Addgene plasmid #8592). GFPT2-deficient H460 clones for population doubling ...
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bioRxiv - Cell Biology 2021Quote: ... was purchased from GeneCopoeia and pcDNA3-Flag-mTOR wt (ID-26603) was procured from Addgene. Mito-BFP ...
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bioRxiv - Biochemistry 2021Quote: ... pcDNA3-FLAG-RIPK3 (Addgene plasmid, http://n2t.net/addgene:78815; RRID: Addgene_78815) was a gift from Jaewhan Song ...
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bioRxiv - Microbiology 2022Quote: ... C-terminal Flag-tagged pCAGGS-SARS2-S-D614 (Addgene plasmid #156420) and pCAGGS-SARS2-S-G614 (Addgene plasmid #156421 ...
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bioRxiv - Molecular Biology 2021Quote: ... pcDNA3-FLAG-VSV-G plasmids (available at Addgene #12259 and #80606) and the GAG-eDHFR vector (serving as a control ...
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bioRxiv - Cell Biology 2021Quote: The Halo-FLAG-FUS in mRFP-C1 vector (Addgene, Watertown, MA) was constructed using pcDNA3 (a gift from Hyun-Woo Rhee ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1+ HDAC7-Flag was a gift from Eric Verdin (Addgene #13824)[71] ...
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bioRxiv - Molecular Biology 2022Quote: ... The MYB-FLAG construct was obtained from Addgene (catalog #66980 [30]).
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bioRxiv - Cell Biology 2023Quote: ... or S170A-ABHD17B-FLAG along with pMD2.G (Addgene plasmid # 12259) and psPAX2 (Addgene plasmid # 12260 ...
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bioRxiv - Plant Biology 2023Quote: ... a C-terminal 3×FLAG® epitope tag (pICSL50007; Addgene #50308), and 3′ UTR and terminator sequences from Agrobacterium tumefaciens nopaline synthase (AtuNOST ...
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bioRxiv - Cell Biology 2023Quote: SH-SY5Y cells stably overexpressing LAMP-1-Flag-RFP (Addgene; #102931) and stained with CellMask Green (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCDH-Flag-c-MYC was a gift from Hening Lin (Addgene plasmid # 102626 ...
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bioRxiv - Immunology 2023Quote: pcDNA3-N-Flag-NLRP3 plasmid encoding mouse NLRP3 (Addgene plasmid # 75127) was a gift from Bruce Beutler61 ...
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bioRxiv - Neuroscience 2023Quote: ... Flag-HA-USP48 (# 22585) and GFP-p65 (# 23255) were from Addgene. All constructs were confirmed by DNA sequencing and protein expression.
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bioRxiv - Systems Biology 2024Quote: ... JNK2α2 and ASK1 were amplified from pCDNA3 Flag Jnk2a2 (Addgene #13755) and pCMV6-Myc-MAP3K5 (ASK1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was sub-cloned in FLAG-HA-pcDNA3.1- (Addgene; 52535) to generate N-terminally Flag-HA tagged MED4 cDNA ...
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bioRxiv - Immunology 2023Quote: ... pIRESneo-FLAG/HA-EYFP (RRID: Addgene_10825, Gift from Thomas Tuschl 77) and HCMV ORF Expression Library 48 ...
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bioRxiv - Cell Biology 2023Quote: 1205Lu cells stably expressing pTrip-CMV-GFP-Flag-cGas (Addgene 86675) and pLBS.CAG-NLS-mScarlet (Addgene 129336 ...
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bioRxiv - Physiology 2019Quote: ... pLKO.1 scrambled shRNA or Sirt4 shRNA was transfected in HEK293 T cells with packaging plasmids pMD2.G (Addgene plasmid # 12259) and psPAX2 (Addgene plasmid # 12260) ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected with 500 ng of equimolar pooled SCN1A_h1b sgRNAs (Table 4) and 500 ng dCas9p300Core (Addgene, plasmid #61357) using Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2021Quote: ... Real time analysis of NFAT4-GFP nuclear translocation in response to 10 µM Cch stimulation was calculated using the equation: Quantification of ER Ca2+ store depletion and refilling was measured by transfecting parental and MCU-KO HEK293 cells with red R-CEPIA1er (Addgene: #58216) using Lipofectamine 24 hrs prior to imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... integrating lentivirus for both Cas9 and sgRNA was generated by overnight transfection of adherent HEK293 cells with either the Cas9 or sgRNA vector and the packaging vectors psPax (Addgene #1226) and pMD2g (Addgene #12259) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lentiviral particles containing the CD19 CAR element were produced by lipofectamine-based co-transfection of HEK293 cells with 3rd generation packaging plasmids pMD2.G (Addgene, #12259), pMDLg/pRRE (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... pAAV_hsyn_NES-his-CAMPARI2-F391W-WPRE-SV40 was a gift from Eric Schreiter (Addgene plasmid # 101061)31 ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Bioengineering 2021Quote: ... We created 2 bacterial expression vectors: pET28-His-MBP-NLS-RfxCas13d-NLS-HA (Addgene 171586) produces the protein RfxCas13d-sTag after cleavage of the N-terminal His-MBP fusion partner ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: A plasmid encoding obligate dimeric TGT was cloned from the TGT-His plasmid (Addgene #138201) using DNA HiFi Assembly (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... A1AT/ SERPINA1 and PAI1/ SERPINE1 cDNAs were amplified from SERPINA-bio-His plasmid (Addgene #52182) and SERPINE1-bio-his (Addgene #52077 ...
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bioRxiv - Molecular Biology 2020Quote: ... strains of interest were transformed with a plasmid containing His-tagged SUMO (Smt3-Hisx7) (Addgene) under the control of a copper inducible promoter ...
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bioRxiv - Microbiology 2022Quote: ... 500 ml LB culture of pCDFDuet-1-6×His-SARS-CoV-2-NSP7/NSP8 (Addgene)-transformed E.coli BL21 DE3 strain was induced by isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid pET28HIS-hAPE1 (for WT His-APE1 protein) was a gift from Primo Schaer (Addgene plasmid #70757 ...
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bioRxiv - Neuroscience 2023Quote: ... and the injections of AAV5-hSyn-NES-his-CaMPARI2-WPRE-SV40 (2.5x10^12gc/ml Addgene 200nl measured using a Nano Injector ...
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bioRxiv - Pathology 2024Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
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bioRxiv - Cell Biology 2020Quote: ... pcDNA3.1-Flag-CLASPIN was a gift from Michele Pagano (Addgene plasmid #12659). Primers containing restriction sites were used to amplify cDNAs for subcloning and primers with mutations or deletions were used for site-directed mutagenesis (SDM) ...
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bioRxiv - Developmental Biology 2021Quote: ... using the linearized plasmid pST1374-NLS-flag-linker-Cas9 (Addgene 44758 45) as a template ...
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bioRxiv - Cancer Biology 2020Quote: The HA-FLAG-hKDM5A.dn3 (#1048) was a gift from William Kaelin (Addgene plasmid # 14799 ...
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bioRxiv - Biochemistry 2021Quote: ... pLPC-puro-N-Flag was a gift from Titia de Lange (Addgene plasmid # 12521 ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP-C1-FLAG-Ku80 (Addgene #46958, gift from Dr. S. Jackson, (26)) ...
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bioRxiv - Genomics 2020Quote: ... The pCMV-FLAG LAP2 plasmid was a gift from Joan Massague (Addgene plasmid #15738 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were transfected with pRK5-TGFβRI-FLAG plasmid (Addgene, #14833) using Lipofectamine 3000 transfection kit ...
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bioRxiv - Biochemistry 2021Quote: The FLAG-HSF-1 plasmid was purchased from Addgene (ID 32537, RRID:Addgene_32537), which was originally established by Dr Stuart Calderwood (40) ...
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bioRxiv - Biochemistry 2021Quote: ... The pcDNA3 Flag HA plasmid was purchased from Addgene (ID 10792, RRID:Addgene_10792), which was originally established by Dr William Sellers ...