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Citations for Addgene :
251 - 262 of 262 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... lentivirus was produced in HEK293FT cells transfected with NOCT Δ(2-15)-3F pCW57.1 and the pRSV-Rev (Addgene #12253), pMD2.G (Addgene #12259) ...
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bioRxiv - Neuroscience 2021Quote: ... P14-15 WT and CYLD KO mice were anesthetized and injected with 0.2 μL AAV2-hSyn-EGFP (Addgene #50465-AAV2) into the CA1 hippocampus (AP ...
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bioRxiv - Neuroscience 2023Quote: EnvA-enveloped ΔGL rabies viruses were rescued in 15 cm plates as described23 using genome plasmids pRVΔGL-4Flpo (Addgene 98040) and pRVΔGL-4Cre (Addgene 98039) ...
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bioRxiv - Immunology 2023Quote: ... Stable transfection was performed by transfecting T2 cells with 4.5ug of HLA cDNA in the pSBbi-GP backbone and 0.5ug of pCMV(CAT)T7-SB100[15] (Addgene plasmid # 34879), followed by puromycin selection.
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced in HEK293T cells by transfecting a 10 cm plate with 15 µg packaging plasmid psPAX2(Addgene), 5 µg envelope plasmid pMD2.G (Addgene) ...
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bioRxiv - Developmental Biology 2023Quote: Transgenic animals were generated by injecting transgenes (15 ng μl-1 each) mixed with a co-injection marker pRF-4 (120 ng μl-1) (Addgene) expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng μl-1 of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were transfected with 5 μg (in 10-cm dish) or 12.5 μg (in 15-cm dish) of pCMV-VSV-G expression plasmid (Addgene, Plasmid #8454) using Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2023Quote: We used AAV1-hSyn-GCaMP6f (2 × 1013 vg/mL Penn Vector Core/Addgene; diluted 1:8 to 1:15 in saline) for experiments involving two-photon calcium imaging of V1 layer 2/3 cells ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression construct containing the target sequence 5’-GGTTTGGTTGATTTCTGCAC-3’ was obtained using a PCR-fusion technique and the PCR product was injected at 15 ng/μL together with 50 ng/μL pDD162 (eft-3prom::Cas9, a gift from Bob Goldstein, Addgene plasmid #47549). After injection ...
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bioRxiv - Cell Biology 2023Quote: ... pcDNA3.1-myc-OGA(1-400) and (344)pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)15 (gift from Christina Woo; Addgene plasmid 168095 and 168197). Additionally ...
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bioRxiv - Microbiology 2020Quote: Lentivirus-derived VLPs were produced by transfecting 10 cm dishes of HEK293T LentiX cells with lentiviral packaging plasmids encoding Gag/Pol (pMDLg/pRRE, 15 µg) and Rev (pRSV-REV, 6 µg) (Addgene plasmids 12251 and 12253) together with vectors expressing SARS-CoV-2 Spike (13 µg ...
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bioRxiv - Neuroscience 2022Quote: ... Each mouse received bilateral injections (15 nl/ hemisphere at a rate of 15 nl/min) of either excitatory DREADD virus pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene Cat# 50476-AAV5, titer: 1.7x1013) or control virus pAAV-CaMKIIa-EGFP (Addgene Cat# 50469-AAV5 ...