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Citations for Addgene :
351 - 400 of 436 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) cells using the plasmid pRSFDuet-1/CylLL/CylM-2 (Addgene ID #208759), following the method previously reported.9 For the preparation of lanthionine standard ...
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bioRxiv - Systems Biology 2024Quote: ... coli origin of replication and ampicillin resistance gene were PCR amplified from pCRISPRyl (Addgene #70007) (Schwartz et al. ...
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bioRxiv - Pathology 2024Quote: ... BL21(DE3)-RIL Escherichia coli cells were transformed with pJ4M-TDP43-MBP-His (Addgene 104480) and grown on LB/Kanamycin/Chloramphenicol plates then used to inoculate 2L of TB/Kan/Cam/2g dextrose ...
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bioRxiv - Cell Biology 2020Quote: ... lentiviral vector Optopatch (a kind gift from Adam E. Cohen group 21,22 acquired through Addgene, plasmid # 62984), was introduced in hiPS-CMs ...
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bioRxiv - Molecular Biology 2022Quote: ... and the pSLQ1651-sgTelomere(F+E) was a gift from Bo Huang & Stanley Qi (Addgene plasmid # 51024) (Chen et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Rc-CMV-Cyclin E (#8963) and lentiviral constructs pLB (#11619) and pLKO (#8453) were purchased from Addgene. shRNA oligos for Spy1 and a scrambled control were ligated into the pLKO and pLB vectors ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... human CaV3.3 (a1Ic-HE3-pcDNA3 also from Dr E. Perez-Reyes, Addgene #45810 (Gomora et al. 2002) in combination with green fluorescent protein.
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs for Decorin and E-cadherin (Gene ID: 999) were cloned into modified pHA-N1 (#86049, Addgene) and pcDNA3-neo-Strep-flag-Cterm (#102645 ...
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bioRxiv - Cancer Biology 2024Quote: The pCC_01 – hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE plasmid (Addgene #139086) was used for CRISPR screen and validation ...
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bioRxiv - Biophysics 2022Quote: HEK 293T cells were co-transfected with either pmNG-Mpro-Nter-auto-NLuc or pmNG-Mpro-Nter-auto-L-NLuc plasmid along with either pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro (Mpro WT) (Addgene plasmid # 141370) or pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro (Mpro C145A ...
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bioRxiv - Biochemistry 2021Quote: ... Nsp14 was subcloned into a modified pBIG1a vector containing a pLIB-derived polyhedrin expression cassette to either contain an N-terminal 3xFlag-6His tag (sequence: MDYKDHDGDYKDHDIDYKDDDDKGSHHHHHHSAVLQ-nsp14) or no tag to generate SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164). Baculoviruses were generated and amplified in Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Templates used to create the PCR fragments were pTwist-EF1alpha-SARS-CoV-2-S-2xStrep (gift from Nevan Krogan; Addgene plasmid #141382), pCEP4-myc-ACE2 (gift from Erik Procko ...
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bioRxiv - Microbiology 2023Quote: The ISRE-Luc reporter plasmid (32) (25 ng) was either transfected alone or co-transfected with a plasmid expressing SARS-CoV-2 genes NSP13 (Addgene, cat. 141379) (25 ng) ...
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bioRxiv - Biophysics 2023Quote: ... a pQE-30/pcl-ts ind+ plasmid containing a His6-small ubiquitin-like modifier (SUMO) SARS-CoV-2 nsp12 and untagged nsp7 and 8 (Addgene no. 160540) was transformed into Escherichia coli BL21 cells (Agilent) ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... pseudo-type lentiviruses coated with the SARS-CoV-2 S protein harboring the vector pCMV14-3X-Flag-SARS-CoV-2 S (a gift from Zhaohui Qian (Addgene plasmid #145780) were prepared by co-transfecting HEK293T cells with psPAX2 (a gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Microbiology 2024Quote: The viral 5′UTR reporter constructs were synthesized using GeneArt and cloned into pLV-SARS-CoV-2 5′UTR-Luciferase (Addgene Cat#191480)32 using NdeI and BsaBI ...
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bioRxiv - Immunology 2019Quote: ... coli O1:K1:H7 (ATCC, Manassas, VA) was transformed with the pGEN-luxCDABE plasmid (Addgene #44918) by electroporation using a Micropulser (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain was transformed with the pBAD::mRFP1 plasmid (Addgene plasmid #54667; Campbell et al., 2002) or the pZsGreen vector (cat ...
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bioRxiv - Biophysics 2021Quote: ... coli expression vector with an N-terminal 6xHis tag followed by an MBP tag (Addgene #29654) by ligation independent cloning (LIC) ...
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bioRxiv - Immunology 2023Quote: ... coli containing the pMF230 plasmid containing a constitutively active promoter and eGFP were ordered from Addgene and cultivated on 100 uM ampicillin LB agar plates ...
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bioRxiv - Microbiology 2023Quote: ... coli LC-E18 and the pSGRNA plasmid was a gift from David Bikard (Addgene plasmid # 115924) and has been described previously (Cui et al. ...
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bioRxiv - Biochemistry 2022Quote: ... coli and subcloned in-line with his-tagged MBP construct (2CT-10 vector, Addgene plasmid #55209). Recombinant MBP-EndoH fusion was expressed in and purified from E ...
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bioRxiv - Biophysics 2023Quote: ... coli expression vectors encoding either no tag (UC Berkeley Macrolab vector 2A-T, Addgene ID 29665) or an N-terminal TEV protease-cleavable His6-tag (UC Berkeley Macrolab vector 2B-T ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli BW25113 carrying pDY118A using a donor plasmid (pKAW086, Figure S3, Table S2, Addgene ID 217967) containing an editing cassette comprised of kanR flanked by regions of homology to the genome (Table S6) ...
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bioRxiv - Immunology 2021Quote: ... and the third exon of NCR3 (conserved in 3 of the 4 longest isoforms of NCR3 with transcript ID ENST00000376073) were designed and cloned into the lentiCRISPR V2 vector (Addgene #52961, sgRNA sequences in Table S1). Lentiviruses were produced in HEK293T cells using standard laboratory protocols ...
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bioRxiv - Cell Biology 2019Quote: To generate clonal cell lines that are deficient for SURF4, a sgRNA targeting SURF4 exon 2 (SI Appendix, Table S1) was cloned into the PX459 plasmid (Addgene: 62988, a gift from Feng Zhang) as previously described (95) ...
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bioRxiv - Microbiology 2020Quote: ... gRNAs specific for UBE4B exon 10 were designed through the E-CRISP web server (http://www.e-crisp.org/E-CRISP/) and expressed by pLentiCRISPRv2-puro (a gift from Feng Zhang; Addgene). Guide sequence function was analyzed using the Surveyor Mutation Detection Kit (Integrated DNA Technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... human CaV3.2 (a1Ha-pcDNA3 was a gift from Dr E. Perez-Reyes, Addgene #45809 (Cribbs et al. 1998), human CaV3.3 (a1Ic-HE3-pcDNA3 also from Dr E ...
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bioRxiv - Immunology 2021Quote: ... an envelope deficient HIV-1 dual reporter construct that was cloned by recombination of the pNL.luc.R-E-plasmid (NIH AIDS Reagent Program) and the fully infectious pNL4-3 mCherry luciferase plasmid (Addgene) [24 ...
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bioRxiv - Microbiology 2022Quote: ... pGBW-m4252984 (SARS-CoV-2 E [envelope]) was a gift from Ginkgo Bioworks (Addgene plasmid #153898; http://n2t.net/addgene:153898; RRID:Addgene_153898). MLV-gag/pol ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA oligonucleotides were inserted into the U6>sgRNA(F+E) vector which was a gift from Lionel Christiaen (Addgene plasmid # 59986 ; http://n2t.net/addgene:59986 ; RRID:Addgene_59986) 52 ...
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bioRxiv - Molecular Biology 2023Quote: Lentiviral constructs coding for TLR (#31482) and I-SceI with donor e-GFP (#31476) were purchased from Addgene. To avoid the confounding effect of NHEJ on the repair of I-SceI-induced DNA breaks ...
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bioRxiv - Cancer Biology 2023Quote: ... PGL2 E-cad (108) Ebox Mut-Luc (kindly provided Dr. Eric R. Fearon, Addgene plasmids 19291 and 19290), and 0.025 μg of pRL-TK (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... mori Gr9 (Genbank Accession EU769120.1) was cloned into a pEG BacMam vector (Addgene plasmid #160451; from E. Gouaux)29 following an N-terminal Strep-tag II30 ...
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bioRxiv - Cell Biology 2021Quote: Plasmids encoding the SARS-CoV-2 open reading frames proteins and eGFP control were a kind gift of Nevan Krogan (Addgene plasmid #141367-141395). Plasmids were acquired as bacterial LB–agar stabs ...
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bioRxiv - Cell Biology 2019Quote: sgRNAs targeting several genes and several non-targeting sgRNAs (listed in SI Appendix, Table S1) were cloned into the pLentiCRISPRv2 plasmid (Addgene:52961, a gift from Feng Zhang (41) as previously described (42) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli codon optimized dSpyCas9 was PCR amplified from vector pdCas9 (gift from Luciano Marraffini, Addgene plasmid #46569) (43 ...
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bioRxiv - Cell Biology 2021Quote: ... The adenovirus was constructed by subcloning the existing E-cadherin tension sensor into the pShuttle-CMV vector (Addgene, 16403), followed by recombination into the pAdEasy1 vector (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... to measure BLA ACh levels (Fig. 2A-E + S2.1-S2.2) or 0.5 µL of AAV1 Syn-FLEX-GCaMP6s-WPRE-SV40 (Addgene, Watertown, MA) to measure BLA principal cell calcium dynamics (Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... We followed the standard principles to avoid off-target effects when choosing between multiple gRNA targets for each PE and cloned into the modified U6.3>gRNA.f+e backbone (Addgene #99139) as described in 54 ...
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bioRxiv - Molecular Biology 2022Quote: sgRNAs targeting selected MYC binding motifs (E-boxes) were designed using transCRISPR and cloned into lentiCRISPR_v2 vector (Addgene #5296126) (Table 2) ...
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bioRxiv - Genomics 2024Quote: ... were combined with CROPseq-Puro-F+E plasmid (containing the sgRNAs) or dCas9-mCherry-ZIM3-KRAB plasmid (Addgene 154473) and transfected using Lipofectamine™ 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... the open reading frame was amplified by PCR from the pDONR223 SARS-CoV-2 NSP5 plasmid (Addgene, #141259, a gift from Fritz Roth [32]), including an ATG start codon in the forward primer and a TAA stop codon in the reverse primer (MPro_Fw ...
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bioRxiv - Immunology 2020Quote: ... pcDNA3-sACE2(WT)-Fc(IgG1) and pcDNA3-SARS-CoV-2-S-RBD-sfGFP plasmids were gifts from Erik Procko (Addgene plasmids #145163 and #141184). pGBW-m4134096 encoding for SARS-CoV-2 M protein was a gift from Ginkgo Bioworks (Addgene plasmid #152039).
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bioRxiv - Biochemistry 2021Quote: ... coli BL21(DE3) RIL from the pET-derived vector 14-B (a gift from S. Gradia; Addgene 48308) in LB medium individually ...
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bioRxiv - Microbiology 2022Quote: ... coli K-12 strain MG1655 that had been transformed with retron-TIR from Shigella dysenteriae NCTC2966 (Addgene #157883) or an empty vector control (pACYC184 ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180; http://n2t.net/addgene:79180; RRID:Addgene_79180) (Jarmander et al. ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS 2-6-3 were transduced with pLenti CMV rtTA3 Blast (w756-1, plasmid #26429; Addgene, gift from E. Campeau) for the expression of rtTA ...
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bioRxiv - Microbiology 2020Quote: ... or human ACE2 ectodomain (containing a C-terminal His tag) were made according to the E and F sections of the pLKO.1 Protocol from Addgene (http://www.addgene.org/protocols/plko/) ...
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bioRxiv - Immunology 2022Quote: ... against the respective genes were designed with e-crisp.org [76] and cloned into the expression vector LentiCRISPRv2 (Feng Zhang, Addgene #52961). Lentiviral particles were produced by transiently transfecting HEK 293T cells with a total of 15 µg DNA consisting of the plasmids pCMV-dr8.91 (coding for HIV gag-pol) ...