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Citations for Addgene :
501 - 550 of 1010 citations for SARS Coronavirus Envelope Protein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The pCMV14-3X-Flag-SARS-CoV-2 S plasmid was a gift from Zhaohui Qian (Addgene plasmid # 145780 ...
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bioRxiv - Immunology 2021Quote: ... Greene) and pCMV14-3X-Flag-SARS-CoV-2 S (a gift from Zhaohui Qian, Addgene #145780) at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA ...
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bioRxiv - Biochemistry 2023Quote: ... SARS-CoV-2 Mac1 without CFP-tag was cloned and expressed from pNH-TrxT (Addgene #173084).
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bioRxiv - Molecular Biology 2021Quote: ... coli cells containing the plasmid pCAS were obtained from Addgene (#60847). Plasmid pCAS (8.7 Kb ...
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bioRxiv - Biochemistry 2022Quote: ... coli NiCo21(DE3) cells were prepared and transformed with pMSP2N2 (Addgene) using a standard New England BioLabs (NEB ...
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bioRxiv - Immunology 2022Quote: ... coli transduced with a SpyCatcher003-mi3 expression plasmid (Addgene, Watertown, MA) were lysed with a cell disrupter in the presence of 2 mM PMSF ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were transformed with arabinose inducible EGFP-pBAD plasmid (Addgene #54762) overnight at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... coli colonies with the pET28a-EGFP-CNA35 gene (Addgene, Plasmid #61603) were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180 ...
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bioRxiv - Cell Biology 2023Quote: ... at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G: Addgene 12259; pRSV-Rev: Addgene 12253; pMDLg/pRRE: Addgene 12251). 4.5μg of lentiviral donor plasmid was added to the mixture and incubated for 20 min with 10μL of polyethylenimine (PEI ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S-mEmerald construct was made by cloning the mEmerald sequence (Addgene, Plasmid #53976) to the C-terminal end of the SARS CoV-2 S-protein in the pCG expression vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.5 μg of SARS-CoV-2 Spike plasmid (a gift from Fang Li, Addgene plasmid #145032) using Lipofectamine 3000 transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... and are also available on Addgene (pLVX-EF1alpha-SARS-CoV-2-nsp14-2xStrep-IRES-Puro, Addgene #141380). Nsp10-Flag plasmid was a gift from the Ott lab.
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bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2 S HexaPro plasmid was a generous gift from Jason McLellan (Addgene plasmid # 154754) (19) ...
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bioRxiv - Immunology 2021Quote: Plasmid pCMV14-3X-Flag-SARS-CoV-2 S was a gift from Zhaohui Qian (Addgene plasmid # 145780)68 ...
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bioRxiv - Microbiology 2022Quote: ... UL123 was also cloned into pLVX-EF1alpha-SARS-CoV-2-nsp1-2XStrep-IRES-Puro (Addgene plasmid #141367) in place of the SARS-CoV-2-nsp1-2XStrep cassette ...
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bioRxiv - Microbiology 2023Quote: ... along with a luciferase gene with SARS-CoV-2 packaging sequence PS9 into 293T cells (Addgene 177942). Plasmids were gifts from Jennifer Doudna ...
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bioRxiv - Immunology 2023Quote: ... the SARS-CoV-2 S 6P expression vector was a gift from Jason McLellan (Addgene plasmid #154754). The secreted protein was captured by Strep-Tactin affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... pCAGGS-SARS-CoV-2 S D614G (# 156421) and pcDNA3.1 vectors were obtained from Addgene (Watertown, MA, USA), and Invitrogen (Waltham ...
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bioRxiv - Molecular Biology 2024Quote: The plasmid pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro (Addgene plasmid #141387 from Nevan Krogan) [12] was used for the overexpression of the accessory proteins ORF6 ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-E-Syt1 was a gift from Pietro De Camilli (Addgene plasmid # 66830) (41) ...
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bioRxiv - Developmental Biology 2023Quote: ... Bot oligo – 5’-aaacTTGGCACTCCATTAGATCCG-3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253; pMDLg/pRRE: Addgene #12251, all gifts from Didier Trono) and 750ng of lentiviral transfer plasmid encoding the transgene of interest after a 15 minute incubation of these plasmids with 10μL of polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2024Quote: ... Lentivirus was produced from pLenti-SV40-T-tsA58 (abm cat# LV629) as previously described (51) using the VSV envelope from pMD2.G (Addgene cat#12259, gift from Didier Trono) and the packaging plasmid pCMV delta R8.2 (Addgene cat # 12263 ...
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bioRxiv - Biochemistry 2019Quote: ... coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (BL21DE3) expressing GST tagged anti-GFP nanobody (Addgene plasmid #61838) (Katoh et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli BL21(DE3) was co-transformed with the plasmids pAC-BETAipi (Addgene), pCDFDuet-OsD27-trAtCCD7 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 transformed with the pTAT-Cre plasmid (#917, Addgene plasmid #35619) were grown for 16 hours in LB containing 100 µg/mL kanamycin ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli MG1655 strain bearing the mutagenesis machinery encoded in the pORTMAGE3 (Addgene Plasmid Number 72678 ...
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bioRxiv - Biophysics 2019Quote: ... coli K12 MG1655 genome or the pET21b-RL027A (Addgene, Cambridge, MA, USA), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... coli expression plasmid obtained as a gift from Niels Geijsen (Addgene #62731) (D’Astolfo et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Escherichia coli expression plasmids used in this study were acquired from Addgene, made possible through a gift by Cheryl Arrowsmith ...
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta(DE3) harbouring NPLOC4 and UFD1 (pAB720 and Addgene #117107, respectively) were cultured in LB as described above ...
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bioRxiv - Immunology 2021Quote: SARS CoV-2 pseudotyped lentiviruses were produced by transfecting the 293T cells with the pLenti-Puro vectors (Addgene) expressing Luciferase or β-Galactosidase ...
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bioRxiv - Immunology 2021Quote: ... The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867 ...
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bioRxiv - Immunology 2021Quote: ... The bacterial expression construct for full-length SARS-CoV-2 nucleocapsid was a gift from Nicolas Fawzi (Addgene plasmid # 157867; http://n2t.net/addgene:157867; RRID:Addgene_157867)69 ...
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bioRxiv - Immunology 2022Quote: ... pBOB-CAG-SARS-CoV2-S-HA was a gift from Gerald Pao (Addgene plasmid # 141347; http://n2t.net/addgene:141347; RRID:Addgene_141347). pCAGGS-SARS2-S-FKO (C-flag ...
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bioRxiv - Molecular Biology 2022Quote: All engineered SARS-CoV-2 S variant ectodomains were designed based on the HexaPro gene (Addgene plasmid #154754) containing stabilizing proline mutations at positions F817P ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Plant Biology 2024Quote: ... for Level 1 and 3 and pEven1-4 (pCsA-E, Addgene plasmids # 136067-136070) for Level 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 (DE3) pLysS cells transformed with pET23b-FB-GFP (Addgene plasmid 129593), induced with IPTG at O.D ...
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bioRxiv - Cancer Biology 2023Quote: ... coli cells were transformed with the pET28a-His6-KEAP1 vector (Addgene plasmid #62454). Colonies were grown in LB broth containing 50 μM ZnCl2 and 50 μg/ml kanamycin at 37 °C until cultures reached an OD600 of 0.6-0.8 and then were allowed to cool to room temperature ...
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bioRxiv - Molecular Biology 2022Quote: The lentiviral vectors pLVX-EF1alpha-IRES-Puro-2xStreg-SARS-CoV-2 (Nsp6, Nsp8, M) (Addgene plasmids #141395, #141372, #141374) were transfected into the HEK293T cells with packaging plasmids ...
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bioRxiv - Microbiology 2021Quote: ... The pcDNA-FLAG-V5-Nsp10/14/16 vectors were constructed from pDONR223 SARS-CoV-2 Nsp10 (Cat. # 141264, Addgene), Nsp14 (Cat ...
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bioRxiv - Molecular Biology 2023Quote: pDONR207 SARS-CoV-2 NSP1 was a gift from Fritz Roth (Addgene plasmid # 141255; http://n2t.net/addgene:141255; RRID: Addgene_141255). NSP1 coding sequence was cloned into the mammalian expression pCDNA5-FRT/TO-2xSTREP-3xHA vector ...
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bioRxiv - Cell Biology 2019Quote: ... SBP-EGFP-E-cadherin and APPL1-mCherry were obtained from Addgene (#65292 and #27683, respectively).
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bioRxiv - Neuroscience 2019Quote: ... region of homology to plasmid pSLQ1651-sgTelomere(F+E) (Addgene #51024; (Chen et al., 2013)) ...
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bioRxiv - Cancer Biology 2022Quote: ... The sgRNA was cloned using the BbsI site of lentiviral vector pLentiCRISPR-E (Addgene, 78852) as described (https://media.addgene.org/cms/filer_public/6d/d8/6dd83407-3b07-47db-8adb-4fada30bde8a/zhang-lab-general-cloning-protocol-target-sequencing_1.pdf) ...
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bioRxiv - Immunology 2023Quote: ... Platinum-E retroviral packaging cells were transfected transiently with modified pMIG retroviral plasmids (Addgene, #9044) or a second-generation anti-hCD19 CAR construct (MSCV-myc-CAR2A-Thy1.1 ...
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bioRxiv - Microbiology 2022Quote: ... coli MG1655 into a His-SUMO-TEV (HST) plasmid backbone (Addgene product number: 48313). Each HST construct was grown in LB to an OD600 of 0.4 at 37°C ...