Labshake search
Citations for Addgene :
451 - 500 of 1366 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... we co-transfected pcDNA3.1-SARS2-Spike or pcDNA3.1-SARS2-SpikeD614G (see above) with pcDNA3.1-ACE2 (Addgene 1786) at a 1:1 ratio (Spike ...
-
bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032);34 this gene was cloned into a modified pcDNA 3.1 backbone (Clontech-Takara ...
-
bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032).
-
bioRxiv - Synthetic Biology 2023Quote: ... that have reached ~80-90% confluency with the second generation pHR plasmid carrying the desired transgene (Supplementary Table S2) and the vectors encoding for the packaging proteins (pCMVR8.74; gift from Didier Trono (Addgene plasmid # 22036 ...
-
bioRxiv - Neuroscience 2023Quote: ... The human CD68 promoter and enhancer (hCD68, ∼800 bp) was subcloned from pcDNA3-hCD68prm (Addgene #34837). The mouse F4/80 promoter (mF4/80 ...
-
bioRxiv - Developmental Biology 2023Quote: ... the lentiviral packaging plasmids 800 ng pMD2.G (was a gift from Didier Trono, Addgene #12259) and 2400 ng psPAX2 (was a gift from Didier Trono ...
-
bioRxiv - Developmental Biology 2020Quote: ... Bot oligo – 5’ aaacGGGTGAGACCCATGTATTTc3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral construct pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry (Addgene plasmid #101282), pLL5.0-eGFP-CAAX (Addgene plasmid #187252 ...
-
bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human DLK1 ectodomain expression vector was obtained from Addgene (DLK1-bio-His, RRID:Addgene_51876) (Sun et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... A plasmid expressing His-TEV-ubiquitin G76C was acquired from Addgene (Watertown, MA) and the protein was purified using Talon beads (Takara Bio Inc. ...
-
bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
-
bioRxiv - Molecular Biology 2021Quote: ... The expression vector used for the pseudovirion SARS CoV2-19 was from Addgene (pCDNA 3.1_Spike_Del19 #155297). The packaging vectors and Lenti ZsGreen were from Addgene (pHIV-Zsgreen #18121 ...
-
bioRxiv - Immunology 2021Quote: ... pTWist-WIV1-CoV (a gift from Alejandro Balazs (Addgene plasmid # 164438; http://n2t.net/addgene:164438; RRID:Addgene_164438), or pHDM-MERS-CoV Spike and a luciferase/GFP reporter (pHAGE-CMV-Luc2-IRES542 ZsGreen-W ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were virally infected with 0.5-1 µl AAV mixture per slice (containing AAV9-Camk2a-Cre at 2 × 1012 vg/ml (1:1000 dilution, Addgene and rAAV8-DIO-CBA-pAAIP2-mEGP at 4.2 x 1012 vg/ml ...
-
bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... The plasmid encoding Control-TAG is available on Addgene (Addgene #138209, pcDNA3.1- (empty)-TAG) ...
-
bioRxiv - Cell Biology 2021Quote: ... for a Golgi tag and mVenus-N1 (color variant of Addgene plasmid #54843) for an untagged version.
-
bioRxiv - Cancer Biology 2022Quote: ... maintaining the DDK-MYC tag and then into Pentr4FLAG (Addgene Cat. no. 17423) before cloning into the lentiviral DOX-inducible destination vector pLIX403 (Addgene Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 pmol of PCR repair template amplified from pSNAP-tag plasmid (Addgene #101135) or mTagBFP2 plasmid (Addgene #75029 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The SMASh tag was removed and replaced with 2xHA dTAG derived from Addgene plasmid 9179224 ...
-
bioRxiv - Developmental Biology 2020Quote: ... CRISPRi gRNAs were generated targeting Noggin and Chordin (Table S2) using the Broad Institute GPP Web Portal and cloned into the gRNA-CKB (Addgene) following the previously described protocol36 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transformed by electroporation and selected on pre-grown bacteria with the appropriate drug 48 (Table S2; most plasmids are available from Addgene).
-
bioRxiv - Cell Biology 2024Quote: ... 3 (Table S2) and were ordered from IDT with BbsI flanking sites for cloning into either pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138 for single cell sorting or pSpCas9(BB)-2A-Puro (PX459 ...
-
bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032) 39 ...
-
bioRxiv - Microbiology 2023Quote: ... Mutagenised and barcoded spike sequence templates were then added into MluI and XbaI digested lentivirus backbone (Addgene #204579) using HiFi reaction (NEB E2621L) ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli cells containing the plasmid pCAS were obtained from Addgene (#60847). Plasmid pCAS (8.7 Kb ...
-
bioRxiv - Biochemistry 2022Quote: ... coli NiCo21(DE3) cells were prepared and transformed with pMSP2N2 (Addgene) using a standard New England BioLabs (NEB ...
-
bioRxiv - Immunology 2022Quote: ... coli transduced with a SpyCatcher003-mi3 expression plasmid (Addgene, Watertown, MA) were lysed with a cell disrupter in the presence of 2 mM PMSF ...
-
bioRxiv - Developmental Biology 2023Quote: ... coli colonies with the pET28a-EGFP-CNA35 gene (Addgene, Plasmid #61603) were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli were transformed with arabinose inducible EGFP-pBAD plasmid (Addgene #54762) overnight at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... coli expression vector pAIDA1 was a gift from Gen Larsson (Addgene plasmid # 79180 ...
-
bioRxiv - Cell Biology 2020Quote: ... EGFP-E-Syt1 was a gift from Pietro De Camilli (Addgene plasmid # 66830) (41) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bot oligo – 5’-aaacTTGGCACTCCATTAGATCCG-3’) were cloned into U6.3>gRNA.f+e (#99139, Addgene) and electroporated at a concentration of 1.5 ug/ul ...
-
bioRxiv - Bioengineering 2019Quote: ... TGFB1-bio-His (proTGFβ) which was a gift from Gavin Wright (Addgene plasmid # 52185) [17] and HA-OVOL2 (OVOL2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
-
bioRxiv - Biochemistry 2022Quote: ... pET29b-IPP1-His was a gift from Sebastian Maerkl & Takuya Ueda (Addgene plasmid # 124137). 15µL of Lemo21(DE3 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
-
bioRxiv - Cell Biology 2023Quote: ... purified from bacteria cells transformed with the plasmid pET-28b-RfxCas13d-His (Addgene 141322), was kindly provided by JP Concordet ...
-
bioRxiv - Biochemistry 2023Quote: GST-HRAS and His/MBP-KRAS were purchased from Addgene (#55653 and # 159546, respectively). GST-KRAS was created with standard Gibson Assembly (NEB ...
-
bioRxiv - Immunology 2022Quote: ... and a 1xHA tag was created using pSL1394 as a base plasmid (Addgene # 129522). A homology-directed repair (HDR ...
-
bioRxiv - Microbiology 2020Quote: ... was replaced with PspCas13b with HIV NES and HA tag (from pC0046 (Addgene #103862)) to generate pC0068-PspCas13b-HA for PspCas13b-HA purification.
-
bioRxiv - Genomics 2022Quote: ... and either pENN.AAV.TBG.PI.N-FLAG-tag mSTAT5bCA.WPRE.bGH (see above) or pENN.AAV.TBG.PI.ffLuciferase.RBG (firefly luciferase expressed from TBG promoter; Addgene # 105538). Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat ...
-
bioRxiv - Synthetic Biology 2022Quote: ... into the TfR-sfGFP-myc tag-SpyCatcher003 plasmid (Keeble et al. [55]. Addgene #133451) through Gibson Assembly (primers listed in Table S3) ...
-
bioRxiv - Microbiology 2021Quote: ... SpyCatcher/Spy-tag and SUMO containing plasmids were purchased from Addgene (#133449 and #111560).
-
bioRxiv - Biochemistry 2021Quote: ... and pGAT2 (N-terminal polyhistidine and GST tags; Addgene #112588; last three genes (61)) by Twist Bioscience ...
-
bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...
-
bioRxiv - Cell Biology 2023Quote: ... the GFP fluorescent tags of ITGB3-GFP (gift from Jonathan Jones – Addgene plasmid #26653) and GFP-Talin1 (gift from Anna Huttenlocher – Addgene plasmid #26724 ...
-
bioRxiv - Cell Biology 2023Quote: The sequence coding for Halo tag was amplified from pSEMS-Halo7Tag-hFis (111136 Addgene) vector by using primers (5’-GCAATTCGATATGGGATCCGAAATCGGTACTGGCTTT CC-3’ and 5’-GGCCTCGAGATTAACCGGAAATCTCCAGAGTAG-3’).
-
bioRxiv - Cell Biology 2023Quote: ... or a pcDNA3.1-based plasmid containing a C-terminal 3xFLAG-V5 tag (Addgene 87063) for all other variants ...