Labshake search
Citations for Addgene :
301 - 350 of 1188 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Microdomain targeting was accomplished using N-terminal fusions of tags to the matrix using 2xCOX8A (tag corresponds to Cox8A N-terminal residues 1-25, from Addgene 136470), the IMS using SMAC (residues 1-59 ...
-
bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... The guide sequence (r4g9, Table S1) was cloned into pX459 vector from Addgene (#62988) and 8ug of the vector was used for mESC transfection ...
-
bioRxiv - Genomics 2023Quote: ... oligos containing the CRISPR sequence (table S1) were inserted into pWS082 (Addgene plasmid #90516), the template plasmid of sgRNA cassette ...
-
bioRxiv - Bioengineering 2022Quote: ... Anti-CD19 and anti-Spike scFv amino acid sequences were obtained from Addgene plasmids #79125 and #155364 ...
-
bioRxiv - Molecular Biology 2022Quote: ... pcDNA3.1-SARS2-Spike was a gift from Dr Fang Li (Addgene plasmid # 145032); pcDNA3.1-ACE2-GFP was a gift from Dr Utpal Pajvani (Addgene plasmid # 154962) ...
-
bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 expressing different spike genes or VSV-G (pMD2.G (Addgene #12259)) using Fugene®HD tranfection reagent (Promega) ...
-
bioRxiv - Developmental Biology 2021Quote: ... the pET-28b-RfxCas13d-His vector (Addgene, Plasmid #141322) was used for RfxCas13d protein production (Bon Opus Biosciences ...
-
bioRxiv - Biochemistry 2020Quote: ... The FLAG-His sequence in payload plasmid pKM491 (Addgene) was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... pET-His-GST-tev-LIC (Addgene #29655, Gradia Lab), pGEX-4T-3-mR7BD (Addgene #79149 ...
-
bioRxiv - Biophysics 2023Quote: ... with an N-terminal hexahistidine tag and T7 tag in pRSET plasmid was gift from was a gift from Yasushi Saeki (Addgene plasmid # 110313)46 ...
-
bioRxiv - Cell Biology 2023Quote: ... conjugated with the Rhotekin Rho Binding domain (RBD)-GST fusion protein (produced from Addgene plasmid #15247), for 1 hour at 4 °C ...
-
bioRxiv - Pathology 2020Quote: ... Lentiviral vector pLV-mCherry and vesicular stomatitis virus glycoprotein (VSV-G) expression vector pMD2.G were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 S gene (GenBank ...
-
bioRxiv - Biochemistry 2023Quote: ... at AgeI and NheI sites and fusing PCR amplified CIB1 and spTN (Addgene plasmid # 153002, (Cho et al., 2020a)) using Gibson assembly ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ubiquitin with HA tag vector was from Addgene (#18712).
-
bioRxiv - Bioengineering 2022Quote: ... and Hemagglutinin (HA) Tag sequences were obtained from Addgene plasmid #85437 ...
-
bioRxiv - Biophysics 2022Quote: ... N-terminal hexahistidine tag fused construct (Addgene plasmid #98669) with a tobacco etch virus (TEV ...
-
bioRxiv - Molecular Biology 2022Quote: ... The FKBP12F36V tag (dTAG) was obtained from Addgene (#91797). ESCs were transfected at approximately 70% confluency in a 6-well plate with 0.5 µg of guide plasmid and 2 µg of targeting construct using Lipofectamine 3000 ...
-
bioRxiv - Neuroscience 2023Quote: ... VEGF TAG and/or PylRS/tRNApyl (Addgene #105830 from29). TCO*A (SiChem ...
-
bioRxiv - Biochemistry 2023Quote: ... and C-terminal SNAC42 and hexahistidine tags (#191551, Addgene). This yielded final expressed sequences as ...
-
bioRxiv - Biochemistry 2023Quote: ... and C-terminal SNAC42 and hexahistidine tags (#191551, Addgene). This yielded final expressed sequences as ...
-
bioRxiv - Biochemistry 2022Quote: ... Expression constructs for various cytoskeleton or organelle marker proteins were obtained from Addgene (S1 Table). For bacterial expression of His6-tagged CAHS proteins ...
-
bioRxiv - Developmental Biology 2021Quote: sgRNA and shRNA oligonucleotides (Table S1) were cloned into lentiCRISPR v2 (Addgene plasmid no. #52961) and into pSMP vector (Addgene plasmid no ...
-
bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
-
bioRxiv - Cell Biology 2019Quote: His-tagged ubiquitin construct was obtained from Addgene (plasmid: #18712). siRNA#1 resistant RNF168-mCherry plasmid and RNF168 siRNAs were gift from Jiri Lucas (1) ...
-
bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
-
bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
-
bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
-
bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
-
bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
-
bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
-
bioRxiv - Microbiology 2020Quote: The integrins subunits were overexpressed in CHO cells line with the following plasmids: Alpha 5 integrin-GFP (Addgene plasmid# 15238)50 ...
-
bioRxiv - Neuroscience 2022Quote: ... Gqi5 and mouse A1 receptor or human D2 receptor were established by transfecting CHO cells with pCAG-cyto-RCaMP (Addgene), pME-Gqi5 (Yamashiro et al*** ...
-
bioRxiv - Cell Biology 2022Quote: ... Stable CHO cell lines expressing CRT-GFP or CRT(Y108F)-GFP were obtained by co-transfection with pBABE-puro (Addgene) and selection with puromycin.
-
bioRxiv - Cancer Biology 2021Quote: ... VRK1WT was further cloned into PLX305(C-TAG) (Addgene #91798) and VRK1WT ...
-
bioRxiv - Genetics 2022Quote: ... The 3XFLAG-tag was cloned from pLP15 (Addgene plasmid #55180) with 5’ KpnI and 3’ EcoRI sites (primers LC127 and 128 ...
-
bioRxiv - Neuroscience 2023Quote: ... respectively13 and produced AAV-CaMKIIα-GLuc-no tag (Addgene 149502) and AAV-CaMKIIα-GLuc-SERCaMP (Addgene 149503) ...
-
bioRxiv - Developmental Biology 2023Quote: ... an amplified PCR product from pCAG-TAG (Addgene, Plasmid #26771) was cloned into the KpnI-NotI sites of pcDNA3.1-H2B mCherry-poly(A83 ...
-
bioRxiv - Immunology 2022Quote: ... pBOB-CAG-SARS-CoV2-S-HA was a gift from Gerald Pao (Addgene plasmid # 141347 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Other plasmids used (for details see excel file S1): pLenti Lifeact-iRFP670 BlastR43 (Addgene Plasmid #84385); pGIZ-PuroR-IRES-GFP_shRNA anti Rab11a/b (Dharmacon) ...
-
bioRxiv - Microbiology 2021Quote: ... pVAX1-SARS2-S1/S2-mut and pVAX1-SARS2-S_D614G) or VSV-G (pMD2.G Addgene #12259). The cell culture supernatants were harvested 24-72 h post transfection followed by addition of fresh media and again after 48-72h ...
-
bioRxiv - Molecular Biology 2021Quote: ... or MGMT (Supplementary Table S1) were annealed and ligated into BsmBI-linearized lentiCRISPRv2 vector (Addgene, #52961). Other plasmids have been described previously (8,17) ...
-
bioRxiv - Microbiology 2023Quote: pT7-S1/HER2nhd (no head domain) was constructed using pBacT7S1-T3D (Addgene, (Kobayashi et al., 2007) as a backbone plasmid ...
-
bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
-
bioRxiv - Cell Biology 2021Quote: ... and pCMV3 nCoV2019 Spike (D614 variant) C-FLAG Hygro was obtained from Addgene (Teddington, UK). Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs ...
-
bioRxiv - Immunology 2021Quote: ... the RBD expression vectors were co-transfected into Expi293F cells together with the secreted BirA-Flag plasmid (Addgene) in the presence of 100 μM biotin.
-
bioRxiv - Biophysics 2020Quote: ... containing N-terminal 6-His-WT-p53 (1-303) (Addgene, 24859), was used to express p53 ...
-
bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
-
bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
-
bioRxiv - Cell Biology 2019Quote: We generated Schizosaccharomyces pombe strains with SNAP-tag (Addgene plasmid 87024) or mEGFP (Addgene plasmid 87023 ...