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Citations for Addgene :
1 - 50 of 512 citations for Recombinant Rat Ace2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: HIS-tagged HER2 (Addgene #16257) was expressed using the Expi293™ expression system (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids for the expression of myc-tagged human ACE2 (pCEP4-myc-ACE2, Addgene No. 141185), 8his-tagged monomeric sACE2 (ACE2 a.a ...
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bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Cell Biology 2019Quote: ... His-tagged SEPT5 plasmid was constructed by PCR amplifying SEPT5 from pCMV-Myc-tagged SEPT5 (Addgene plasmid # 27272 ...
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bioRxiv - Cell Biology 2019Quote: His-tagged ubiquitin construct was obtained from Addgene (plasmid: #18712). siRNA#1 resistant RNF168-mCherry plasmid and RNF168 siRNAs were gift from Jiri Lucas (1) ...
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bioRxiv - Cell Biology 2020Quote: ... Histidine-tagged ubiquitin (pCI-His-hUbi, #31815) and Ha-tagged p62 (HA-p62, #28027) plasmids were purchased from Addgene. DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen ...
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bioRxiv - Cancer Biology 2020Quote: ... V5/His-tagged pLenti-puro-LacZ and pLenti-puro-ARID1A were obtained from Addgene.
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bioRxiv - Synthetic Biology 2022Quote: ... C-terminal His-tagged eGFP plasmid was from our lab stock (Addgene, No. 178422)32 ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged wild-type and D135S AlkB plasmids were obtained from Addgene (#79050 and #79051) and proteins were purified by a Ni-NTA column followed by cation exchange ...
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bioRxiv - Molecular Biology 2020Quote: ... strains of interest were transformed with a plasmid containing His-tagged SUMO (Smt3-Hisx7) (Addgene) under the control of a copper inducible promoter ...
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bioRxiv - Biophysics 2020Quote: ... overnight dialysis and subsequent Sumo-tag cleavage by human sumo protease (His-tagged SenP1; Addgene #16356) 44 were performed in 50 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2022Quote: A plasmid construct containing only the maturation protein and his-tagged coat protein dimer (Addgene # 179156) was transformed into Rosetta2™ (DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... coli and subcloned in-line with his-tagged MBP construct (2CT-10 vector, Addgene plasmid #55209). Recombinant MBP-EndoH fusion was expressed in and purified from E ...
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bioRxiv - Microbiology 2021Quote: The lentiviral vector expressing ACE2 (pRRL.sin.cPPT.SFFV/ACE2, Addgene 145842) has been described (Rebendenne et al. ...
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bioRxiv - Microbiology 2024Quote: ... A549 cells stably expressing ACE2 (A549-ACE2) were generated by transducing with ACE2-encoding (generated using Addgene plasmid no ...
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bioRxiv - Genetics 2020Quote: ... we amplified human ACE2 (hACE2) from pcDNA3.1-ACE2 (Addgene 1786) and cloned it into a lentiviral transfer pLEX vector carrying the hygromycin resistance gene using Gibson Assembly Master Mix (NEB E2611L) ...
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bioRxiv - Biophysics 2020Quote: ... Two plasmids containing full-length untagged Npl4 and C-terminal His-tagged Ufd1 were purchased from Addgene. Npl4 fragments were expressed in pET-28a vector with an N-terminal His-SUMO tag ...
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bioRxiv - Biochemistry 2022Quote: Codon optimized plasmids encoding the individual His-tagged PBRM1 bromodomain constructs were received from Nicola Burgess-Brown (Addgene plasmid numbers 38999 ...
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bioRxiv - Microbiology 2020Quote: An ACE2 lentivirus expression CS(ACE2)IB vector was constructed by inserting a cDNA encoding an unaltered ACE2 (Addgene:1786) or an catalytically inactive ACE2 mutant (ACE2-H374N&H378N ...
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bioRxiv - Molecular Biology 2020Quote: ... ACE2 (Addgene Plasmid #145171) was cloned into a pCD5 expression vector with SacI and BamHI restriction enzymes ...
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bioRxiv - Neuroscience 2020Quote: ... Human His-tagged SETD1A expression pET28-SETD1A-MHL plasmid and its control pET28-MHL were gifts from Cheryl Arrowsmith (Addgene plasmid # 32868 and #26096 ...
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bioRxiv - Biophysics 2024Quote: The expression plasmid for HIS-tagged AavLEA1 was obtained from the Addgene plasmid repository [pET15b-AavLEA1 was a gift from Claude Férec (Addgene plasmid # 53093)]52 The expression plasmid for HIS-tagged RvLEAMshort was made by inserting a truncated cDNA from pEThT-RvLEAM49 [pEThT-RvLEAM was a gift from Takekazu Kunieda (Addgene plasmid # 90033)] ...
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bioRxiv - Microbiology 2024Quote: ... To produce lentivirus carrying ACE2 expression cassette 14.2ug of pLENTI-ACE2 (ADDGENE, Watertown, MA, USA), 16ug of pCMVdR 8.2 (ADDGENE ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting chimera genes for the light chain and the His-tagged heavy chain of the Fabs were separately cloned into the pCAGEN vector (a gift from Connie Cepko (Addgene plasmid #11160)) 34 ...
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bioRxiv - Biochemistry 2020Quote: ... coli RP hk339-GFP (monomeric His-tagged Kif5B kinesin motor domain) expression plasmid was a gift from Ron Vale (Addgene plasmid #24431).
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bioRxiv - Cell Biology 2022Quote: ... pCEP4-myc-ACE2 (Addgene plasmid #141185). PBcmv-229E-wt_WRPE(39) ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry-tagged Rabaptin5 and YFP-tagged Arf6 were from Addgene. Flag-ErbB3 cDNA was subjected to site-directed mutagenesis (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The full length of ACE2 sequence from pCEP4-myc-ACE2 (a gift from Erik Procko, Addgene plasmid #141185) was cloned into pHR-CMV lentiviral expression vector (a gift from A ...
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bioRxiv - Microbiology 2021Quote: Stable ACE2 expressing HeLa cells (HeLa-ACE2) were generated by transducing HeLa-dCas cells with pLENTI_hACE2_HygR (Addgene, #155296) followed selection with hygromycin ...
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bioRxiv - Immunology 2020Quote: Soluble human ACE2 with an Fc tag was constructed by PCR amplifying ACE2 (residues 1-615) from Addgene plasmid #1786 (a kind gift from Jesse Bloom ...
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bioRxiv - Microbiology 2020Quote: ... A plasmid containing the ACE2 gene (Addgene) was digested with Nhe I and Kpn I and the fragment was ligated into the similarly digested pLenti-III-HA vector plasmid ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.WPRE (Addgene 145842), or by an IRES-neomycinR cassette ...
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bioRxiv - Microbiology 2020Quote: The ACE2 expression plasmid (Addgene, plasmid #1786) was received in bacteria and purified with a maxiprep kit (Zymogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... For overexpressing ACE2 (Addgene, Appendix Table 2) in HPLFs ...
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bioRxiv - Bioengineering 2024Quote: ... ACE2 plasmid is from Addgene (Cat#1786). All the other plasmids used in this study are cloned by following the standard protocol of Infusion HD (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) kindly provided by Caroline Goujon (Addgene plasmid # 145842) (Rebendenne et al ...
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bioRxiv - Microbiology 2021Quote: HeLa cells were seeded onto glass coverslips and transfected with either myc-ACE2 (pCEP4-myc-ACE2 from Addgene Plasmid #141185), ACE2 (Daly et al. ...
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bioRxiv - Microbiology 2020Quote: ... The ACE2 gene (obtained from Addgene [Catalog #: 1786]) was placed under the control of the human cytomegalovirus (hCMV ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.IRES-neo.WPRE (Addgene 145840), or by an IRES-hygromycin R cassette ...
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bioRxiv - Microbiology 2020Quote: ... to generate pRRL.sin.cPPT.SFFV/ACE2.IRES-hygro.WPRE (Addgene 145841), respectively ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 sgRNA plasmid or Cas9Bsd plasmid (Addgene #68343) (45 ...
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bioRxiv - Immunology 2023Quote: A lentiviral construct containing human ACE2 (Addgene 155295) or mScarlet (Addgene 85044 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and (RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE(MT129)(Addgene#145840). 293/hACE2 population were then maintained in culture medium containing G418 to obtain 293 cells that stably expressing recombinant hACE2 ...
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bioRxiv - Neuroscience 2020Quote: ... pEZYmyc-His (Addgene plasmid #18701 ...
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bioRxiv - Microbiology 2021Quote: ... Derivatives of HEK-293T cells expressing ACE2 were generated by transducing HEK-293T (ATCC, CRL-3216) cells with ACE2 (Addgene, #145839). Cells were used as single cell clone was derived by limiting dilution from the bulk populations ...
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bioRxiv - Microbiology 2020Quote: ... cDNA used to generate ACE2 positive cells was constructed as follows: the ACE2 PmeI cDNA fragment obtained from the plasmid hACE2 (Addgene; #1786) was cloned in pMD2iPuror opened in EcoRV.
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells transiently co-expressing individual GFP-tagged RUSH reporter and GalT-mCherry were cultured in complete DMEM supplemented with16 nM His-tagged streptavidin (in-house purified using Addgene #20860, a gift plasmid from A. Ting)(Howarth et al. ...