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Citations for Addgene :
151 - 200 of 2311 citations for Recombinant Human SPARC like 1 Hevin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Engineering of Luc-tagged HCC1954 cells (HCC1954-Luc) and tumour implantation: the pLenti CMV Puro LUC (w168-1) was purchased from Addgene (#17477) and used in all in vivo experiments ...
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bioRxiv - Biochemistry 2024Quote: ... pEGFP-C1-tagged plasmids containing the exon 1 of HTT with 23 CAG repeats (GFP-Q23: wild-type HTT. Addgene, #40261) or 74 CAG repeats (GFP-Q74 ...
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bioRxiv - Cell Biology 2020Quote: ... The Toronto human knockout pooled library (TKO) Version 1 (Addgene #1000000069) was a gift from Jason Moffat19.
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Molecular Biology 2020Quote: A pET28a vector with sub-cloned cDNA of Hsp53-(1-73) (72R) and the N-terminal His-tag was procured from Addgene, (plasmid #62082) ...
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bioRxiv - Microbiology 2020Quote: ... or human ACE2 ectodomain (containing a C-terminal His tag) were made according to the E and F sections of the pLKO.1 Protocol from Addgene (http://www.addgene.org/protocols/plko/) ...
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bioRxiv - Immunology 2021Quote: ... par-5 and his-1 cDNA were subcloned into pCE-BiFC-VN173 and pCE-BiFC-VC155 plasmids (Addgene, Cambridge, MA), which contain the heat shock promoter Phsp-16.41 ...
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bioRxiv - Cell Biology 2020Quote: ... eIF4B and Ded1 were tagged using the pML104 vector backbone (Addgene: 67638) while eIF4A was tagged using pML107 (Addgene ...
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bioRxiv - Neuroscience 2020Quote: A SEP-tagged version of GluA224 (gift from Roberto Malinow, RRID: Addgene_24001) was inserted under the synapsin promoter in a pCI backbone ...
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bioRxiv - Cell Biology 2019Quote: myc-tagged DBC1 (myc-DBC1) cloned in pcDNA3 was obtained from Addgene, USA ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged NHERF1 originated from Vijaya Ramesh’s laboratory (from Addgene, plasmid 11635). 16E6 point mutants were created using QuikChange primer design (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (BL21DE3) expressing GST tagged anti-GFP nanobody (Addgene plasmid #61838) (Katoh et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag-tagged DAXX DNA were amplified from plasmid Flag-Daxx/pRK5 (Addgene) and assembled into pCDH-EF1α-MCS-IRES-Puro vector (System Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged TurboID (promiscuous BirA mutant, V5-Turbo-NES-pCDNA3, Addgene 107169) and C-FLAG-D20 or EGFP (pEGFP-N1-FLAG ...
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bioRxiv - Cancer Biology 2023Quote: ... HA-tagged ubiquitin (empty vector: pRK5-HA) was obtained from ADDGENE (17608). Control siRNA and siRNA targeting USP7 was obtained from Santa Cruz Biotechnology and added at a final concentration of 30 picomolar.
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bioRxiv - Biochemistry 2023Quote: ... The Myc-tagged strains were then transformed with pRS319 (RRID:Addgene_35459, ref. (79)) to introduce a LEU3 marker for selection ...
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bioRxiv - Neuroscience 2023Quote: ... C-terminally GFP-tagged Mannosidase cDNA was obtained from Addgene (plasmid #160905) and used without additional subcloning ...
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bioRxiv - Cell Biology 2023Quote: ... we used the following emerald-green GFP (emGFP)-tagged vectors from Addgene: BAZ1A (#65371) ...
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bioRxiv - Neuroscience 2023Quote: ... a Streptavidin-binding peptide-FLAG (SFB)-tagged USP7 construct56 (Addgene plasmid #99393) was transfected into HEK293T cells via PEI transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-HA-tagged YTHDC1 plasmid was purchased from Addgene (plasmid # 85167).
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bioRxiv - Microbiology 2022Quote: The SARS CoV-2 papain-like protease (PLpro) domain of Nsp3 was cloned from a doxycycline-inducible piggyBac transposon vector (PB-TAC-ERP2, Addgene# 80478) containing the synthesized full-length Nsp3 from the Wuhan-Hu-1 SARS CoV 2 strain (Alvarez and Yao ...
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bioRxiv - Neuroscience 2020Quote: Recombinant adeno-associated virus carrying the GCaMP6f gene (AAV2/1:hSyn-GCaMP6f) was obtained from Addgene (100837-AAV1) with titer ≥ 1×1012 ...
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bioRxiv - Cancer Biology 2020Quote: ... human PML SUMO-1 mutant and GFP-BLM were purchased from Addgene (#62804 ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-N1 human cofilin-1 S3E (gift from James Bamburg; Addgene 50861), pEGFP-N1 human cofilin-1 S3A (gift from James Bamburg ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-N1 human cofilin-1 WT (gift from James Bamburg; Addgene 50859), pEGFP-N1 human cofilin-1 S3E (gift from James Bamburg ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-N1 human cofilin-1 S3A (gift from James Bamburg; Addgene 50860) cofilin-1 (Garvalov et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Biophysics 2023Quote: ... a pQE-30/pcl-ts ind+ plasmid containing a His6-small ubiquitin-like modifier (SUMO) SARS-CoV-2 nsp12 and untagged nsp7 and 8 (Addgene no. 160540) was transformed into Escherichia coli BL21 cells (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... The recombinant DNA encoding TCRMART-1 was synthesized by GeneScript (Nanjing, China) and ligated into pRRLSIN.cPPT.PGK vector (Addgene, 12252).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with tandem mRFP-GFP fluorescent-tagged LC3 (ptfLC3) (Addgene #21074). 24h post-transfection ...
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bioRxiv - Immunology 2019Quote: ... Expression plasmids encoding EGFP-tagged Rab5a and its mutants were purchased from Addgene, USA ...
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bioRxiv - Physiology 2019Quote: ... GFP-PGC1 plasmid expressing eGFP-tagged mouse PGC1a was acquired from Addgene(50). For in vivo electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2019Quote: ... Histidine-tagged PKA catalytic subunit (a gift from Susan Taylor, Addgene plasmid #14921) and PKI3 were previously described ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-tagged LAMP1 was a gift from Amy Palmer (Addgene plasmid # 45147). The myc-tagged WWOX plasmid was kindly provided by R ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected with plasmids expressing Flag-tagged mTOR (Plasmid #26603, Addgene) together with HA-tagged Raptor (Plasmid #8513 ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged SQSTM1 and RFP-LC3 were gifts from Qing Zhong (Addgene, 28027)20 and Tamotsu Yoshimori (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... C-terminally FLAG-tagged ASXL1 p.G646Wfs*12 and p.Y591X were obtained from Addgene. The MSCV-T2A-Puromycin vectors encoding 3xFLAG-tagged full-length ASXL1 and truncated ASXL1 were generated by PCR amplification followed by sub-cloning using Gibson assembly.
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bioRxiv - Molecular Biology 2024Quote: A pET28b plasmid encoding C-terminal 6xHis-tagged nuclear Pif1 (Addgene plasmid #65047)64 was employed to express and purify Pif1 from E ...