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Citations for Addgene :
101 - 150 of 1103 citations for Recombinant Human KEAP1 His GST & AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Eps15-GFP-His (Addgene #170860 ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-tagged mTOR (Plasmid #26603) and HA-tagged Raptor (Plasmid #8513) were purchased from Addgene. Transfection were performed with the TransIT-X2 Transfection Reagent (Cat ...
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bioRxiv - Immunology 2021Quote: ... The human full length (HFL) gene sequence was subcloned from pcDNA5/FRT/TO HIS HSPA1A (a gift from Harm Kampinga, Addgene plasmid # 19537 ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were generated by sub cloning HA-tagged human TRIM71 and its mutant variants sequences from pMXS-hs-3xHA-TRIM71 (Addgene, no. 52717), pMXs-hs-3xHA-deltaRING-TRIM71 (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... pDsRed-RAB11A WT (for expression of a fluorescently-tagged, wild-type version of RAB11A in human cells) was obtained from Addgene (plasmid 12679). For expression of GFP-HEATR5B from baculovirus ...
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bioRxiv - Cell Biology 2023Quote: ... a donor plasmid pAAVS1-TRE3G-NGN2 was generated by replacing the EGFP sequence with N-terminal flag-tagged human NGN2 cDNA sequence in plasmid pAAVS1-TRE3G-EGFP (Addgene plasmid # 52343). 5μg of pAAVS1-TRE3G-NGN2 ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-tagged cytosolic APEX2 (Addgene, 49386) was then amplified by polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... Myc-tagged DNTCF4 plasmids (Addgene, #32729) were transiently transfected into targeted cells for a total of 24 hours before being subjected to the wound scratch assay.
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bioRxiv - Cell Biology 2022Quote: ... Flag-tagged SMAD4 (#80888, Addgene®), and HA tagged TAZ (#32839 ...
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bioRxiv - Cancer Biology 2023Quote: ... from the expression vector pcDNA3.1/Myc-His(-)-HSulf-2 bearing human Sulf-2 cDNA (a gift from Steven Rosen, Addgene plasmid # 13004) (Morimoto-Tomita et al. ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagD S77L (RagDGDP; Addgene plasmid # 19308), pRK5-HA GST RagD Q121L (RagDGTP ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagB Q99L (RagBGTP; Addgene plasmid # 19303), pRK5-HA GST RagC S75L (RagCGDP ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagB T54L (RagBGDP; Addgene plasmid # 19302), pRK5-HA GST RagB Q99L (RagBGTP ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagD Q121L (RagDGTP; Addgene plasmid # 19309). For all Rag constructs similar levels of transfection efficiency were achieved with ~75% of cells expressing 48 hours after transfection ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagC S75L (RagCGDP; Addgene plasmid # 19305), pRK5-HA GST RagC Q120L (RagCGTP ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagC Q120L (RagCGTP; Addgene plasmid # 19306), pRK5-HA GST RagD S77L (RagDGDP ...
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bioRxiv - Biochemistry 2021Quote: ... N-terminal His6 plus glutathione S-transferase (GST; RRID:Addgene_29707); N-terminal His6 plus small ubiquitin-like modifier (SUMO ...
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bioRxiv - Neuroscience 2022Quote: ... The vector backbone AIRAP9-PSMD2-GST (Addgene no. 21799), was digested using HinDIII and XhoI to remove the PSMD2 CDS and the amplified Zic3 gene was cloned using Infusion kit (Clontech ...
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bioRxiv - Systems Biology 2021Quote: ... The pMEL16 (His-, Addgene 107922) and p414-TEF1p-Cas9-CYC1t (hereafter referred to as P414 ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID ...
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID ...
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bioRxiv - Biochemistry 2021Quote: ... Vector pREXNH3CA used to clone EfrCD in frame with a C-terminal Avi-tag was constructed from pREXNH3 (Addgene #47079) by PCR amplification with 5’ phosphorylated primers pREXNH3(newAvi_5’P)_FW (5’-aga aaa tcg aat ggc acg aaT AAT AAC TAG AGA GCT CAA GCT TTC TTT GA ...
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bioRxiv - Cancer Biology 2023Quote: ... Packaging 293T cells were transfected with KEAP1 single guide RNA (sgRNA) and helper vectors (pMD2.G and psPAX2; Addgene plasmid # 12259 and 12260) using Lipofectamine 3000 reagent (Cat ...
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bioRxiv - Molecular Biology 2023Quote: - recombinant pGEX-4T-3-P53: The human-p53 cDNA was previously cloned in the EcoRI site of pGEX-4T3 (Addgene_79149) under the lac-trp hybrid promoter that is inducible by IPTG ...
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bioRxiv - Cancer Biology 2021Quote: ... The pcDNA FLAG-tagged CRTC2 (#22975) and FLAG-tagged CRTC3 (22976) expression constructs were obtained from Addgene (38).
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bioRxiv - Cancer Biology 2020Quote: ... and RFP-tagged histone H2B (Addgene#26001) expressing plasmids were obtained from Addgene or DNASU as indicated ...
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bioRxiv - Cancer Biology 2020Quote: ... FLAG/HA-tagged AGO1 (Addgene plasmid #10820), AGO2 (Addgene plasmid #10822) ...
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bioRxiv - Cell Biology 2022Quote: ... and HA tagged TAZ (#32839, Addgene®) cloned into pcDNA ...
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bioRxiv - Bioengineering 2022Quote: An Arc plasmid (pGEX-6p1-GST-ArcFL, Addgene plasmid #119877) and a GFP plasmid (pcDNA3-EGFP ...
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bioRxiv - Biochemistry 2024Quote: ... pGEX4T3-GST-Tev was a gift from Robert Sobol (Addgene plasmid # 177142 ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... The cDNAs for GFP-tagged two FYVE domains of mouse HRS and PH domain of human TAPP1 were obtained from Addgene (#140047 and #161985, respectively) and cloned into the pLVX-IRES-puro vector ...
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bioRxiv - Immunology 2023Quote: ... including a N-terminal 6xHis tag and AviTag was synthesized and purchased from Twist Bioscience and subcloned into the pHR-CMV-TetO2_3C-Avi-His6 IRES-EmGFP vector (a gift from A. Radu Aricescu, Addgene plasmid # 113888) by in vivo assembly (IVA ...
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bioRxiv - Cancer Biology 2020Quote: ... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and SERPINE1-bio-his (Addgene #52077) which were gifts from Gavin Wright without signal sequence to prevent cleavage of fused proteins (75) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tag purification (Addgene ID: 139113)
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bioRxiv - Developmental Biology 2021Quote: pET-28b-RfxCas13d-His (Addgene #141322) plasmid containing the T7 promoter was linearized by using NotI restriction site ...
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bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-BMAL1-His (AddGene CN: 31367), pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN ...
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bioRxiv - Developmental Biology 2023Quote: pcDNA3-BMAL1-His (AddGene CN: 31367) were purchased from the Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... N-terminal FLAG-tagged CAPS was created by recombining the human CAPS gene (CAPS/pENTR; DNAsu HscD00514950) into pEZFLAG (Addgene#18700; Guo et al., 2008) using Gateway cloning ...
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bioRxiv - Molecular Biology 2020Quote: To make the plasmid for GST-Rpb1-linker expression (Addgene #138468), the linker region of Rpb1 (a.a ...
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bioRxiv - Molecular Biology 2020Quote: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201 ...
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bioRxiv - Cell Biology 2020Quote: ... GST-p85-S6K1 expression vector48 was obtained from Addgene (Plasmid #8466) and transfected into HEK293A cells using Fugene 6 transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: Bacterial expression vectors for GST-JNK1 (pGEX4T1-3xFLAG-JNK1, Addgene #47574), GST-p38α ...
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bioRxiv - Molecular Biology 2019Quote: ... 1-160 and 1-220 were cloned in pEBG-GST (Addgene). The vector expressing eGFP was described previously (49) ...
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bioRxiv - Cell Biology 2020Quote: ... PTP-PEST was sub-cloned in pENTR4-GST-6P1 (Addgene#17741) lentivirus entry vector backbone at BamH1 and XbaI restriction sites followed by LR clonase mediated gateway recombination in 3rd generation lentivirus destination vector pLenti CMV Puro Dest (Addgene#17452) ...
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bioRxiv - Cell Biology 2020Quote: ... while eIF4A was tagged using pML107 (Addgene: 67639). Both plasmids included an ampicillin resistance gene ...
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bioRxiv - Biochemistry 2021Quote: ... HA tagged USP5 (#22590) was purchased from Addgene and subcloned into pcDNA5 mammalian expression vector ...
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bioRxiv - Cell Biology 2022Quote: ... together with HA-tagged Raptor (Plasmid #8513, Addgene). 36 h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... His14-Tev-tagged SENPEuB protease (Addgene ID #149333) was expressed in E ...
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bioRxiv - Immunology 2019Quote: ... and 6-His tag (Addgene plasmid# 50803) [36] ...