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Citations for Addgene :
151 - 200 of 1364 citations for Recombinant Human GPC3 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... hLamp1-BFP (#1016) plasmid encoding BFP-labeled version of human Lamp1 protein was from Addgene (Cat# 98828). GFP-hRab14.dn3 (#1017) ...
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bioRxiv - Cell Biology 2021Quote: Nfic-HA tagged cDNA was purchased from Addgene (https://www.addgene.org/31403/) and subcloned into the lentiviral vector pLVX-puro using XhoI and XbaI ...
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bioRxiv - Microbiology 2022Quote: ... C-terminal Flag-tagged pCAGGS-SARS2-S-D614 (Addgene plasmid #156420) and pCAGGS-SARS2-S-G614 (Addgene plasmid #156421 ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged PAPR1 WT (PARP1-Flag; #111575) was purchased from Addgene. The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... Envy-tagged strains were engineered by capturing GFP-Envy from Addgene plasmid #60782 using PEP4 or VPH1-specific primers as described (Slubowski et al. ...
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bioRxiv - Molecular Biology 2023Quote: N-terminal histidine tagged wild-type VCP was obtained from Addgene (plasmid #12373 ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-mCherry GST-tagged nanobodies (Addgene #70696, (Katoh et al, 2016)) were expressed and purified according to published protocol (Katoh et al. ...
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Immunology 2021Quote: ... Plasmid expressing the human ACE2 protein with a C-terminal C9 tag was obtained from Addgene (Plasmid 1786). 293T cells were transduced with retroviral particles carrying a pQCXIP vector encoding the gene for the human ACE2 protein ...
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bioRxiv - Cell Biology 2021Quote: ... The mCh-hRab7A (#922) plasmid encoding mCherry-labeled version of human Rab7A protein was from Addgene (Cat# 61804). GFP-hRab5A.dn3 (#966) ...
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bioRxiv - Cell Biology 2021Quote: The RFP-hRab5A.dn3 (#921) plasmid encoding RFP-labeled version of human Rab5A protein was from Addgene (Cat# 14437). The mCh-hRab7A (#922 ...
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bioRxiv - Cell Biology 2024Quote: ... We next used the human EZH2 primary protein sequence available in the addgene for hEZH2 plasmid (#24230, Addgene) and predicted the possible cysteine residues using the GPS-SNO prediction tools ...
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bioRxiv - Cell Biology 2020Quote: ... eIF4B and Ded1 were tagged using the pML104 vector backbone (Addgene: 67638) while eIF4A was tagged using pML107 (Addgene ...
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bioRxiv - Neuroscience 2020Quote: A SEP-tagged version of GluA224 (gift from Roberto Malinow, RRID: Addgene_24001) was inserted under the synapsin promoter in a pCI backbone ...
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bioRxiv - Cell Biology 2019Quote: myc-tagged DBC1 (myc-DBC1) cloned in pcDNA3 was obtained from Addgene, USA ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged NHERF1 originated from Vijaya Ramesh’s laboratory (from Addgene, plasmid 11635). 16E6 point mutants were created using QuikChange primer design (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (BL21DE3) expressing GST tagged anti-GFP nanobody (Addgene plasmid #61838) (Katoh et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag-tagged DAXX DNA were amplified from plasmid Flag-Daxx/pRK5 (Addgene) and assembled into pCDH-EF1α-MCS-IRES-Puro vector (System Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged TurboID (promiscuous BirA mutant, V5-Turbo-NES-pCDNA3, Addgene 107169) and C-FLAG-D20 or EGFP (pEGFP-N1-FLAG ...
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bioRxiv - Biochemistry 2023Quote: ... The Myc-tagged strains were then transformed with pRS319 (RRID:Addgene_35459, ref. (79)) to introduce a LEU3 marker for selection ...
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bioRxiv - Cancer Biology 2023Quote: ... HA-tagged ubiquitin (empty vector: pRK5-HA) was obtained from ADDGENE (17608). Control siRNA and siRNA targeting USP7 was obtained from Santa Cruz Biotechnology and added at a final concentration of 30 picomolar.
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bioRxiv - Neuroscience 2023Quote: ... C-terminally GFP-tagged Mannosidase cDNA was obtained from Addgene (plasmid #160905) and used without additional subcloning ...
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bioRxiv - Neuroscience 2023Quote: ... all NLS tagged RFP constructs with p-EGFP-N1 (Addgene; 6085-1). To qualitatively verify Cre-dependent ArgiNLS variant expression ...
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bioRxiv - Cell Biology 2023Quote: ... we used the following emerald-green GFP (emGFP)-tagged vectors from Addgene: BAZ1A (#65371) ...
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bioRxiv - Neuroscience 2023Quote: ... a Streptavidin-binding peptide-FLAG (SFB)-tagged USP7 construct56 (Addgene plasmid #99393) was transfected into HEK293T cells via PEI transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-HA-tagged YTHDC1 plasmid was purchased from Addgene (plasmid # 85167).
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bioRxiv - Neuroscience 2022Quote: ... a mammalian expression vector encoding an EGFP-human 0N4R tau fluorescent fusion protein was obtained from Addgene (catalog # 46904). This plasmid was developed by the lab of Karen Ashe [16] ...
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bioRxiv - Genetics 2024Quote: Human pcDNA3-FLAG-RBBP5 plasmid used in the protein expression experiments was obtained from Addgene (Cat# 15550, MA, USA). Candidate variants were introduced using QuikChange II Site-directed mutagenesis kit according to manufacturer’s protocol (Agilent ...
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bioRxiv - Biophysics 2020Quote: ... containing N-terminal 6-His-WT-p53 (1-303) (Addgene, 24859), was used to express p53 ...
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bioRxiv - Developmental Biology 2021Quote: ... SpCas9 was expressed from pET28a-Cas9-His (Addgene plasmid number 98158) and purified in our lab ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164) were expressed in baculovirus-infected Sf9 insect cells ...
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bioRxiv - Plant Biology 2024Quote: ... cells harboring the plasmid p2CT-His-MBP-Lbu_C2c2_WT (Addgene No. 83482) were cultivated with 800 ml autoinduction medium (Studier ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with tandem mRFP-GFP fluorescent-tagged LC3 (ptfLC3) (Addgene #21074). 24h post-transfection ...
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bioRxiv - Immunology 2019Quote: ... Expression plasmids encoding EGFP-tagged Rab5a and its mutants were purchased from Addgene, USA ...
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bioRxiv - Physiology 2019Quote: ... GFP-PGC1 plasmid expressing eGFP-tagged mouse PGC1a was acquired from Addgene(50). For in vivo electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were tagged with mEmerald (a gift from Michael Davidson, Addgene #53975; RRID:Addgene_53975), mRuby2 (a gift from Michael Davidson ...
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bioRxiv - Cell Biology 2019Quote: ... Histidine-tagged PKA catalytic subunit (a gift from Susan Taylor, Addgene plasmid #14921) and PKI3 were previously described ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-tagged LAMP1 was a gift from Amy Palmer (Addgene plasmid # 45147). The myc-tagged WWOX plasmid was kindly provided by R ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected with plasmids expressing Flag-tagged mTOR (Plasmid #26603, Addgene) together with HA-tagged Raptor (Plasmid #8513 ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged SQSTM1 and RFP-LC3 were gifts from Qing Zhong (Addgene, 28027)20 and Tamotsu Yoshimori (Addgene ...