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Citations for Addgene :
251 - 300 of 1599 citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were cloned into plasmid 438B (pFastBac His6 TEV cloning vector with BioBrick Polypromoter LIC subcloning, Addgene plasmid #55219). Constructs 438B-INTS3 ...
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bioRxiv - Bioengineering 2023Quote: ... Homemade TEV protease (plasmid was obtained as a gift from David Waugh, Addgene #8827, and purified in-house)(44 ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The resultant PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (Addgene # 29659) using in-fusion cloning kit (Takara ...
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bioRxiv - Biophysics 2023Quote: ... or an N-terminal TEV protease-cleavable His6-tag (UC Berkeley Macrolab vector 2B-T, Addgene ID 29666). For coexpression ...
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bioRxiv - Cell Biology 2024Quote: ... Mis18BP11-490 was cloned from a codon optimised sequence (GeneArt) into the pET His6 MBP TEV (14C, Addgene plasmid #48309 ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence for MZT2A was PCR amplified from pACEBac1-ZZ-TEV-MZT2-3C-mEGFP (a gift from Tarun Kapoor (Addgene plasmid # 178074 ; http://n2t.net/addgene:178074 ; RRID:Addgene_178074)) using the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs used in the procedure were generated by modyfing pKK-RNAi-nucCHERRYmiR-TEV-EGFP (Addgene plasmid #105814). The insert containing CGG repeats within the 5’UTR of FMR1 gene fused with EGFP sequence was derived from 5’UTR CGG 99x FMR1-EGFP (Addgene plasmid #63091) ...
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bioRxiv - Biophysics 2024Quote: ... into a pET His6 TEV LIC cloning vector (a gift from Scott Gradia, Addgene plasmid # 29653; http://n2t.net/addgene:29653; RRID:Addgene_29653). We designed oligomerisation-deficient constructs (D123P ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Cas9 protein was produced by the Weizmann Institute of Science Protein Purification Unit using the pET-28b-Cas9-His (Alex Schier Lab Plasmids, Addgene, Cambridge, MA, United States) as a template ...
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bioRxiv - Cell Biology 2021Quote: GFP-tagged galectin 3 (pEGFP-hGal3 (Addgene, plasmid no. 73080) was mutated using the Q5 site directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Genetics 2019Quote: FLAG tagged ubiquitin was cloned in pCW57.1 vector (Addgene # 41393) between BsrG1 sites and verified through sequencing ...
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bioRxiv - Cell Biology 2023Quote: GFP tagged WT Keratin 8 in pEGFP-N3 (Addgene 6080) was kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... mCherry-tagged CLIMP63 (gift from Gia Voeltz 52, Addgene, 136293),mcherry-FIS1 (gift from Uri Manor53) ...
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bioRxiv - Cancer Biology 2023Quote: ... HA-tagged SOX4 [43] or empty vector control (Addgene 12257). SMARCA4 was overexpressed by WT SMARCA4-sfGFP (Addgene 107056 ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminally AcGFP-tagged SOD1 variants SOD1-WT (Addgene: #264074) and SOD1-G85R (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... Then His14-Avi-SUMOEu1-tagged anti-GFP nanobody (Addgene #149336) was immobilized onto the washed magnetic beads for 30 min with mixing at 4°C using a ratio of 27 μg for every 80 μl beads ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged MSP1D1 and MSPE3D1 (Addgene plasmids #20061 and #20066) were expressed in E ...
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bioRxiv - Biophysics 2019Quote: The cDNAs for protein expression in this study were as follows: human Tau-2N4R (Addgene #16316), human MAP7 (GE Dharmacon MGC Collection #BC025777) ...
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bioRxiv - Cell Biology 2021Quote: ... Corresponding oligonucleotides were annealed and cloned in the pX330 plasmid expressing human SpCas9 protein (Addgene #42230). The donor plasmids were constructed as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... and Caprin-1 were expressed using a pET-derived expression vector (Addgene, pET His6 MBP Asn10 TEV LIC, 1C) in E.coli Rosetta as N-terminal fusions to an N-terminally His6-tagged E.coli maltose-binding protein (MBP) ...
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bioRxiv - Cell Biology 2019Quote: ... pcDNA3.1 TEV (full-length) was a gift from Xiaokun Shu (Addgene plasmid #64276; http://n2t.net/addgene:64276; RRID: Addgene_64276)(To et al. ...
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bioRxiv - Evolutionary Biology 2019Quote: ... CdtB was cloned into the pET His6 TEV vector 2B-T (a gift from Scott Gradia, Addgene plasmid #29666) using sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Molecular Biology 2021Quote: ... The gene fragment for Cas12k was cloned into the bacterial expression plasmid pET-His6-StrepII-TEV LIC (Addgene: #29718). Cas12k ...
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bioRxiv - Biochemistry 2021Quote: ... Jürg Müller (for generation of pcDNA5.1-FRT/TO-puro-(N)GFP-TEV-FLAG-3C-BAP1) or originated from Wade Harper’s laboratory obtained from Addgene (for generation of pcDNA5-FRT/TO-puro-eGFP-BAP1) ...
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bioRxiv - Molecular Biology 2022Quote: ... The gene encoding Cas3/I-G was cloned into pET Strep II TEV LIC cloning vector (1R, Addgene #29664). All the clones were confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the ORFs were cloned into plasmid H6-mCerulean (pET Biotin His6 TEV mCerulean LIC cloning vector, Addgene plasmid #29726). In the second step ...
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bioRxiv - Molecular Biology 2023Quote: ... the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into pET-His6-MBP-TEV (Addgene #29656) by ligation-independent cloning following QB3 Macrolab protocols (https://qb3.berkeley.edu/facility/qb3-macrolab/) ...
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bioRxiv - Microbiology 2023Quote: The construct pFUS ΔIDR-YFP was created by PCR amplifying the fus gene from pGST- TEV-FUS (a kind gift from Dr. Aaron Gitler; Addgene plasmid # 29629; http://n2t.net/addgene:29629; RRID: Addgene_29629) [120] with the addition of XhoI and BamHI sites using primers ...
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bioRxiv - Biochemistry 2023Quote: ... and pET His6 TEV LIC cloning vector (2A-T, a generous gift from the Scott Gardia lab) (Addgene # 29665) using Ligation Independent Cloning to generate the pAKTB-α and pAKTB-β plasmids ...
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bioRxiv - Biochemistry 2023Quote: ... pET-His6 msfGFP-TEV cloning vector with BioBrick polycistronic restriction sites (9GFP) was a gift from Scott Gradia (Addgene plasmid #48287 ...
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bioRxiv - Cell Biology 2023Quote: ... Human NDP52 cDNA was cloned into a pGST2 vector with an N-terminal GST tag followed by a TEV cleavage site (RRID:Addgene_187828). After the transformation of the pGST2 vector encoding GST-TEV-NDP52 in E ...
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bioRxiv - Cell Biology 2023Quote: ... from where it was subcloned into a pGEX-4T1 vector with an N-terminal GST tag followed by a TEV cleavage site (RRID:Addgene_208870). For expression of unlabeled NAP1 in E ...
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bioRxiv - Cell Biology 2023Quote: ... and subcloned into a pGEX-4T1 vector with an N-terminal MBP-tag followed by a TEV cleavage site before wild-type NAP1 (RRID:Addgene_208871), NAP1 delta-NDP52 (S37K/A44E ...
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bioRxiv - Developmental Biology 2021Quote: ... the pET-28b-RfxCas13d-His vector (Addgene, Plasmid #141322) was used for RfxCas13d protein production (Bon Opus Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... The FLAG-His sequence in payload plasmid pKM491 (Addgene) was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... insoluble His-tag purification (Addgene ID: 139126, 139127, 139128)
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bioRxiv - Biochemistry 2020Quote: ... soluble His-tag purification (Addgene ID: 139114, 139115 respectively)
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bioRxiv - Biophysics 2023Quote: ... coli biotin ligase BirA from the T7 promoter (Addgene#109424) in E ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... hLamp1-BFP (#1016) plasmid encoding BFP-labeled version of human Lamp1 protein was from Addgene (Cat# 98828). GFP-hRab14.dn3 (#1017) ...
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bioRxiv - Cell Biology 2021Quote: Nfic-HA tagged cDNA was purchased from Addgene (https://www.addgene.org/31403/) and subcloned into the lentiviral vector pLVX-puro using XhoI and XbaI ...
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bioRxiv - Microbiology 2022Quote: ... C-terminal Flag-tagged pCAGGS-SARS2-S-D614 (Addgene plasmid #156420) and pCAGGS-SARS2-S-G614 (Addgene plasmid #156421 ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged PAPR1 WT (PARP1-Flag; #111575) was purchased from Addgene. The Flag-tagged PARP1 R138C mutant was generated by the site-directed mutagenesis Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... Envy-tagged strains were engineered by capturing GFP-Envy from Addgene plasmid #60782 using PEP4 or VPH1-specific primers as described (Slubowski et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-mCherry GST-tagged nanobodies (Addgene #70696, (Katoh et al, 2016)) were expressed and purified according to published protocol (Katoh et al. ...
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bioRxiv - Molecular Biology 2023Quote: N-terminal histidine tagged wild-type VCP was obtained from Addgene (plasmid #12373 ...
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bioRxiv - Biophysics 2021Quote: ... The pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc) was a gift from Scott Gradia (Addgene plasmid #48285 ...
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bioRxiv - Microbiology 2022Quote: The IMC12 coding sequence was cloned into the pET His6 TEV LIC bacterial expression vector (Scott Gradia, Addgene plasmid #29653) using primers P32-35 ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid 6His-MBP-TEV-huLbCpf1 (plasmid # 90096) (see Figure.1 and Supplementary M3 in the Supplement Data) was purchased from Addgene. After extracted from amplified strains ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids for tetracycline-inducible expression of LAP-Rab34 (LAP consists of GFP, TEV protease site, and S-tag) were constructed by modification of pCW-Cas9 (Addgene #50661 ...