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Citations for Addgene :
1 - 50 of 1717 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... and added undiluted to K562 cells for a final cell concentration of 3-4 x 105 cells/mL for pJT126-based effector recruitment vectors (Addgene #161926) or 1-2 x 105 cells/mL for pCL040-based inducible protein expression vectors (to be deposited on Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Knockout cells generated in 293A and HeLa cells were created with pLentiCRISPRv2 (Addgene, #52961) containing indicated gRNAs (Figure 7-figure supplement 1) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells (#240073, Addgene, USA) were used for viral production ...
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bioRxiv - Biochemistry 2021Quote: ... Electrocompetent cells were prepared from frozen cell stocks and transformed with the pFREE vector (Addgene plasmid #92050 ...
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bioRxiv - Cell Biology 2019Quote: Stable Cas9-expressing cells were generated by transducing BeWo cells with lentiCAS9-blast (Addgene, cat. 52962). To generate TMEM16F/ANO6 knockout cells ...
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bioRxiv - Systems Biology 2020Quote: ... MDCKI + bPAC cells were generated by infecting MDCKI cells with pLenti-PGK-bPAC::NES (Addgene #130267). PKAc expressing cells were obtained by transfecting MDCKI and MDCKI + bPAC cells with pPB-CAG-PRKACA::mRuby2 (Addgene #130268) ...
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bioRxiv - Cell Biology 2019Quote: For generation of lentiviral knockout and overexpression cells HEK293T cells were transfected with psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Immunology 2022Quote: ... KMS11-Cas9+ cells and LP1-Cas9+ cells (transduced with pLX 311-Cas9 construct, Addgene plasmid # 96924) were obtained from the Broad Institute ...
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bioRxiv - Genomics 2022Quote: ... and NIH/3T3 cells (Addgene #138149), as well as doxycycline-inducible nuclease-inactive RfxdCas13d-NLS HEK293FT ...
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bioRxiv - Microbiology 2020Quote: ... and AdEasier-1 cells (Addgene #16399) were a gift from Bert Vogelstein (He et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells harboring plasmid pKM208 (Addgene #13077) were cultured at 30°C to an O.D ...
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bioRxiv - Microbiology 2023Quote: ... Electrocompetent cells containing pREDCas9 (Addgene #71541) were generated as previously described[68] ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli MG1655 K12 cells (Addgene #37854) transformed with reporter ...
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bioRxiv - Immunology 2023Quote: ... Phoenix-Eco cells (Addgene CRL-3214) were used for retrovirus production ...
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bioRxiv - Cell Biology 2020Quote: ... endogenous SETD2 was immunoprecipitated using whole-cell extracts from HEK293T cells expressing mCherry-ACTB (Addgene plasmid #54966). Similarly ...
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bioRxiv - Microbiology 2023Quote: ... IMR90 MxB KO cells were constructed by transducing the cells with lentiviral vector (lentiCRISPR v2; Addgene #52961) containing a gRNA targeting the genomic MxB target sequence (5’-GGTGGTGGTTCCCTGTAACG-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... untransfected HEK293T cells or cells transfected 48 hours earlier with pEGFP-C1 Lifeact-EGFP (Addgene, plasmid #58470) were treated with Wnt5a CM or protein (200 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells and NCI-H1299 cells were infected with pLentiCRISPR V2 viral vector (Addgene, # 52961) in which the sgRNA for human MPC1 gene (5’-AAGTCTCCAGAGATTATCAG-3’ ...
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bioRxiv - Microbiology 2021Quote: Stable ACE2 expressing HeLa cells (HeLa-ACE2) were generated by transducing HeLa-dCas cells with pLENTI_hACE2_HygR (Addgene, #155296) followed selection with hygromycin ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP cells stably expressing dCas9-KRAB were generated by transducing LNCaP cells with Lenti-dCas9-KRAB (Addgene #89567) followed by blasticidin selection ...
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bioRxiv - Molecular Biology 2019Quote: Inducible Cas9 (iCas9) BL cells were established by transducing cells with pCW-Cas9-Blast-based lentiviruses (Addgene #83481) and selecting with Blasticidin ...
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bioRxiv - Cell Biology 2020Quote: ... Vector control cells were created by transfecting C2C12 cells with an empty pBABE-puro vector backbone (#1764, Addgene) and selected and kept in culture medium with 10 μg/mL puromycin.
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bioRxiv - Genomics 2020Quote: FUS-/- cells were generated by transient transfection of U-2 OS cells with pX459 (v2, Addgene plasmid #62988) vectors(Ran et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... A HeLa H2B-mCherry EmGFP-NSD3-L cell line was generated by transfecting EmGFP-NSD3-L cells with a pH2B_mCherry_IRES_puro2 plasmid (Addgene #21045) as described above ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transduced with rtTA (Addgene 20342) and NGN2 (Addgene 99378 ...
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bioRxiv - Biochemistry 2019Quote: ... cells were transfected with control (pcDNA3.1, Addgene) or pcDNA3.1-Myc-eIF4A1 (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines stably overexpressing EβC (Addgene, 24312) were generated by sorting mCherry+ cells ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were transfected with pCMVdR8.91 (Addgene) and pSEW-luc2 (Promega ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with EGFP-CAAX (Addgene) plasmid to mark the membrane ...
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bioRxiv - Microbiology 2023Quote: ... cells carrying pORTMAGE311B plasmid (Addgene no. 120418) were inoculated into 2 ml LB medium plus 50 μg/ml kanamycin and were grown at 37°C and continuous shaking (250 RPM ...
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bioRxiv - Cancer Biology 2023Quote: ... dsRed positive cells (when using Addgene #86004) or GFP positive cells (when using Addgene #195124 ...
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bioRxiv - Systems Biology 2023Quote: ... or pSLIK neo (TM15c6 cells; Addgene #25735) for lentiviral packaging ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transduced with psPAX2 (Addgene, 12260), pVSVg ...
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bioRxiv - Molecular Biology 2021Quote: ... PUS7 and TRUB1 knock out (KO) cell lines were generated by transfecting 293T cells with pLentiCRISPRv2 (Addgene Cat #52961), encoding Cas9 and the following single guide RNAs (sgRNAs) ...
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bioRxiv - Systems Biology 2020Quote: ... PKAc expressing cells were obtained by transfecting MDCKI and MDCKI + bPAC cells with pPB-CAG-PRKACA::mRuby2 (Addgene #130268). Cells were maintained in MEM (Gibco #11095072 ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated single cell forebrain NPCs were plated 1,000,000 cells/well on 12 well plates and transfected with lentiGuide-tdTomato (Addgene #99376) plasmid and selected by hygromycine ...
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bioRxiv - Cell Biology 2020Quote: The PIK3C2A knockout cell line was generated by transfecting HeLa cells with pSpCas9(BB)-2A-GFP (pX458, Addgene #48138) into which a single guide (5’-CACCGAGCACAGGTTTATAACAAGC-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... Cells stably expressing the Mito-DsRed mitochondrial probe were generated by infecting cells with pLV-mitoDsRed (Addgene, plasmid 44386) lentivirus particles with puromycin (2 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 cells were generated by lentiviral transduction of HT29 cells with Lenti-dCas9-VP64-Blast (Addgene, 61425) in the presence of 8 μg/ml polybrene (Santa Cruz ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (1–2 x 105 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene; plasmid #136466 ...
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bioRxiv - Biophysics 2022Quote: Imaging of actin cytoskeleton and microtubule network in life cell experiments was performed using NIH 3T3 stable cell line expressing Lifeact-GFP (Addgene) treated with SiR-tubulin (Spirochrome) ...
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bioRxiv - Cancer Biology 2020Quote: Single-cell clones of Cas9-expressing CALM-AF10 cells were established by cloning in a Lenti-Cas9-2A-Blast vector (Addgene: #73310 ...
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bioRxiv - Microbiology 2022Quote: ... MGAT1 single KO cells were used to generate MGAT1/C1GALT1 DKO cells and MGAT1/UGCG DKO cells with the pLentiSpBsmBI sgRNA Hygro vector (#62205, Addgene). MGAT1/C1GALT1 DKO cells were used to generate MGAT1/C1GALT1/UGCG TKO cells with the lentCRISPRv2 neo vector (#98292 ...
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bioRxiv - Immunology 2020Quote: ... Generation of shRNA IFIT1 cells: 293T cells were co-transfected with psPax2 and pMD2.G (Addgene plasmids #12260 and #12259), as well as pLKO.1 (50 ...
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bioRxiv - Immunology 2021Quote: The Jurkat-NFAT-GFP cell line was generated by transducing Jurkat cells (ATCC, TIB-152) with the pSIRV-NFAT-eGFP plasmid (Addgene, #118031 ...
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bioRxiv - Immunology 2022Quote: A375-MR1-Cas9 cells generated using the previously described cell line (10) and Lenti Cas9-Blast plasmid (Addgene, Cat#52962) were transduced at 0.3 MOI by both part A and B of the pooled Human GeCKO v2 CRISPR library (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... XPA-KO HaCaT cells stably expressing Flag-tagged XPA were generated by transfecting cells with pcDNA4-Flag-XPA (Addgene #22895) and then selecting single clones with geneticin ...
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bioRxiv - Microbiology 2022Quote: ... MGAT1/C1GALT1 DKO cells were used to generate MGAT1/C1GALT1/UGCG TKO cells with the lentCRISPRv2 neo vector (#98292, Addgene). Primers for sgRNA target sites used here are listed in Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... A stably expressing mCherry cell line was utilized for live imaging endothelial cells that was previously developed using pAAVS1-P-CAG-mCh (Addgene).
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bioRxiv - Bioengineering 2023Quote: PC3-luc cells were derived from PC3 cells transfected with the firefly luciferase expressing plasmid from Addgene (Ubc.Luc.IRES.Puro, RRID:Addgene 33307) (22) ...