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Citations for Addgene :
1 - 50 of 1091 citations for PGK Promoter Lin28 Lentivirus since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cells were first infected with lentivirus for PGK-H2B-EGFP (Addgene) and then nucleofected with CRISPR RNPs as needed and plated into 24-well or 12-well plates ...
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bioRxiv - Bioengineering 2021Quote: ... Lin28 (#27080; Addgene); miR302-367 cluster (#98748 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-EmGFP and PGK promoter (a gift from Tyler Jacks (Addgene plasmid # 11586 ...
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bioRxiv - Cell Biology 2023Quote: ... The Lin28 plasmids were purchased from Addgene; Lin28A (#51371) ...
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bioRxiv - Cancer Biology 2022Quote: ... melanoma cells were infected with a lentivirus made using pHAGE-PGK-GFP-IRES-LUC-W (Addgene # 46793) containing coding sequences for green-fluorescent protein and firefly luciferase ...
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bioRxiv - Neuroscience 2020Quote: ... and LIN28 (Addgene plasmids #27077, #27078, and #27080) using previously published protocols (Armstrong et al. ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment of mito-EmGFP and PGK promoter (a gift from Tyler Jacks (Addgene plasmid # 11586; http://n2t.net/addgene:11586; RRID:Addgene_11586)) were prepared by PCR and transferred to a retroviral plasmid backbone (a gift from Fred Gage (Addgene plasmid # 49054 ...
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bioRxiv - Developmental Biology 2023Quote: ... iNPCs were infected with lentivirus expressing mCherry driven by EF1α promoter (EF1a_mCherry_P2A_Hygro, Addgene, # 135003) using spinfection method described above ...
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bioRxiv - Developmental Biology 2022Quote: ... pCXLE-L-MYC-F2A-LIN28 (ML, Addgene ID 27080), pCXLE-hOCT4-shTP53 (Addgene ID 27077) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were immortalized by human telomerase gene (hTERT) using the retrovirus vector pLXSN-hTERT or the lentivirus vector pCLX-PGK-hTERT vector (#114315, Addgene). Retrovirus and lentivirus preparations ...
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bioRxiv - Cancer Biology 2023Quote: ... were established after transduction with a lentivirus carrying the pHAGE PGK-GFP-IRES-LUC-W plasmid (gift from Darrell Kotton, Addgene plasmid # 46793 ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmids for vascular endothelial growth factor A (VEGFA)-EGFP lentivirus production were pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE (Plasmid #89609, Addgene), pMD2.G (Plasmid #12259 ...
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bioRxiv - Molecular Biology 2024Quote: We cloned the vector pME015 containing an mouse PGK promoter driving the expression of a double selection marker HyTK (HyTK amplified from plasmid #11684, Addgene), flanked by FRT and F3 heterotypic recombination sites embedded between Sleeping Beauty 5’ and 3’ inverted terminal repeats (ITRs) ...
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bioRxiv - Neuroscience 2024Quote: ... AAVrg-PGK-Cre (Addgene), AAV-2/1/CAG-Flex-EGFP (Addgene) ...
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bioRxiv - Cancer Biology 2023Quote: ... PGK-H2B-mCherry (21217) and PGK-H2B-eGFP (21210) were purchased from Addgene.
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bioRxiv - Cell Biology 2022Quote: ... and a PGK driven hygromycin selection cassette from MSCV Luciferase PGK-hygro (Addgene 18782) were amplified using primers containing overhangs with the homology sites for GMAP cloning and inserted into a lentiviral vector (LV 1-5 ...
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bioRxiv - Neuroscience 2020Quote: ... AAV-pgk-retro-Cre (Addgene), AAV-hsyn-retro-FLEX-ChR2 (Addgene) ...
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bioRxiv - Cancer Biology 2022Quote: ... pLV-PGK-GFP (Addgene; 19070) was included as a control ...
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bioRxiv - Cancer Biology 2021Quote: ... or PGK-H2BeGFP (Addgene #21210) viruses at high MOI ∼5 to make sure every cell was fluorescently labeled ...
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bioRxiv - Neuroscience 2022Quote: ... AAV-pgk-retro-Cre (Addgene), AAV-flex-GFP (Addgene) ...
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bioRxiv - Cancer Biology 2024Quote: ... or PGK-H2BeGFP (Addgene #21210) were seeded as 50.000 cells/well in 12-well plates ...
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bioRxiv - Cell Biology 2022Quote: pEGFP-IRAlus-Lin28 was a gift from Gordon Carmichael & Ling-Ling Chen (Addgene plasmid # 92347 ...
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bioRxiv - Neuroscience 2020Quote: ... except that an additional lentivirus expressing eGFP under the control of a TET-responsive promoter was included (Addgene Cat # 30130). eGFP-positive neurons were mixed with eGFP-negative neurons in the 96-well plates ...
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bioRxiv - Genomics 2021Quote: ... one set of cells received a lentivirus expressing the Cre recombinase under the control of the hSyn1 promoter (Addgene 86641). This induced expression of the dCas9p300 transgene in the neurons ...
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bioRxiv - Neuroscience 2024Quote: ... i3Ns and iDAs were transduced with lentivirus expressing GCaMP7s driven by the Synapsin promoter (pCDH.rSyn.jGCaMP7s-cloned from pGP-AAV-syn-jGCaMP7s-WPRE, Addgene plasmid #104487) two days before live cell imaging ...
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bioRxiv - Genetics 2020Quote: ... The Clover PGK-HygR and Cherry PGK-BRS were cloned from the published vectors pMK278 (Addgene #72794) and pMK282 (Addgene # 72798) ...
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bioRxiv - Microbiology 2022Quote: HK1 cells that stably express GFP-LMP1 under control of a tetracycline-inducible promoter were created by first being transduced with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; 17492) as previously described in (18) ...
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bioRxiv - Physiology 2024Quote: ... cDNA of wild type and mutant HDAC3 were PCR amplified from corresponding Lentivirus vectors and cloned into sites between T7 promoter and bGH poly (A) signal of pENTR-CMV (Addgene, 32688) plasmids ...
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bioRxiv - Neuroscience 2022Quote: - AAV-pgk-Cre (Addgene 24593, retrograde serotype), here referred to as AAVretro-Cre ...
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bioRxiv - Synthetic Biology 2020Quote: ... The luciferase-based GO system with sgRNA in the same vector was obtained from Addgene (pLenti-mU6-Luc2GO-PGK-Neo, Addgene #136905).
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bioRxiv - Microbiology 2021Quote: Cells stably expressing shRNA of different genes under the control of a tetracycline-inducible promoter were created by first transducing SNB-19 cells with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; number 17492). The cells underwent selection with media containing 10 μg/mL of blasticidin (Invivogen ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells were generated by infecting cells with lentivirus containing a Cas9-P2A-GFP expression cassette under a doxycycline inducible promoter as described previously (Addgene plasmid #85400)50 ...
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bioRxiv - Bioengineering 2022Quote: Previously described lentiviral transfer constructs pHR-PGK-antiCD19-synNotch-Gal4VP64 and pHR-Gal4UAS-IRES-mC-PGK-tBFP were obtained from Addgene. The PRAME-specific HSS1 TCR construct was provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... pLV-dCas9-KRAB-PGK-HygR (Addgene plasmid #83890) was described previously50 ...
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Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liverbioRxiv - Immunology 2024Quote: ... and pLenti PGK V5-LUC Neo (Addgene #21471) and the viral packaging envelope plasmids pMD2g (Addgene #12259 ...
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bioRxiv - Microbiology 2023Quote: ... is required to induce expression from inducible TRE3G promoter in the lentivirus backbone in the presence of doxycycline (see Addgene #204579 plasmid structure). Note ...
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bioRxiv - Cancer Biology 2023Quote: ... cloned into pLenti PGK Neo DEST (pLenti PGK Neo DEST (w531-1) a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19067) by using the Gateway LR Clonase II Enzyme Mix (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: ... the pLenti PGK V5-LUC Neo (Addgene plasmid # 21471) was obtained from Addgene (30,31) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and EGFP from PL-SIN-PGK-EGFP (Addgene #21316) with Gibson Assembly primers ...
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bioRxiv - Cell Biology 2022Quote: ... pLenti-PGK-ER-KRASG12V was purchased from Addgene (35635).
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bioRxiv - Genetics 2024Quote: ... pCCL-PGK-SPdCas9-BFP-DNMT1(dCas9-DNMT1) (Addgene#66818) pHRdSV40-NLS-dCas9-24ξGCN4-v4-NLS-P2A-BFP-dWPRE (Addgene#60910) ...
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bioRxiv - Bioengineering 2023Quote: ... and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898) used for reprogramming experiments have been described previously17 ...
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bioRxiv - Microbiology 2024Quote: ... pCDH-PGK was a gift from Kazuhiro Oka (Addgene plasmid # 72268 ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus: psPAX2 (Addgene, 12260) with VSVg envelop plasmid pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2022Quote: pEGFP-IRAlus-Lin28 was a gift from Gordon Carmichael & Ling-Ling Chen (Addgene plasmid # 92347; http://n2t.net/addgene:92347; RRID:Addgene_92347).
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bioRxiv - Cancer Biology 2023Quote: ... PDTO were transduced with lentiviral supernatants for pKLV2-U6-gRNA5(Empty)-PGK-BFP-2A-GFP-W and pKLV2-U6-gRNA5(gGFP)-PGK-BFP-2A-GFP-W (Addgene plasmids # 67979 and# 67980) [32] ...
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bioRxiv - Neuroscience 2022Quote: ... PGK CFP (subcloned from CMV ARF6Q67L-CFP, Addgene plasmid ##11387), CMV mScarlet-LC3B (subcloned from EGFP-LC3B ...
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bioRxiv - Neuroscience 2022Quote: ... AAV-pgk-Cre was a gift from Patrick Aebischer (Addgene plasmid # 24593 ...
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bioRxiv - Cell Biology 2022Quote: ... and the pcDNA3-DEST or pLenti-PGK-Hygro (Addgene, 19066) destination vector ...
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bioRxiv - Cancer Biology 2024Quote: ... T1-160-Cas9 cells expressing either PGK-H2BmCherry (Addgene #21217) or PGK-H2BeGFP (Addgene #21210 ...