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Citations for Addgene :
151 - 200 of 1354 citations for PCSK9 Human V474I G670E HEK293 His HA Avi since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: CRISPR mediated knock-outs were performed by transducing HEK293 cells with LentiCRISPRV2 (Addgene #52961) lentivirus expressing sgRNAs targeting genes of interest (FASTKD5_sg1 ...
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bioRxiv - Neuroscience 2020Quote: ... BirA-HA and HaloTag constructs were PCR-amplified from pcDNA3.1-MCS-BirA(R118G)-HA (a gift from Kyle Roux, Addgene #36047) (Roux et al. ...
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bioRxiv - Cell Biology 2022Quote: ... FLVCR1-HA and SLC5A7-HA were subcloned into pLJM1-EGFP-PGK-mCherry vector backbone (a derivative of pLJM1-EGFP, Addgene #19319 ...
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bioRxiv - Genetics 2020Quote: pDEST-FLAG-HA-USP5 and pDEST-FLAG-HA-OTUD5 were gifts from Wade Harper (Addgene plasmids # 22590 and # 22610(42). OTUD5 patient mutations (G494S ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs encoding cyclin D1 WT and cyclin D1 (T286A) were PCR amplified from pcDNA cyclinD1 HA and pcDNA cyclinD1 HA T286A (Addgene plasmids #11181 and # 11182 ...
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bioRxiv - Developmental Biology 2023Quote: ... Nphs2 was amplified from adult mouse kidney cDNA and cloned into the MCS-13X Linker-BioID2-HA plasmid (MCS-13x Linker-BioID2-HA was a gift from Kyle Roux (Addgene plasmid # 80899 ...
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bioRxiv - Plant Biology 2023Quote: ... or into the pAG416GPD-ccdB-HA vector (Alberti et al., 2007) (pAG416GPD-ccdB-HA was a gift from Susan Lindquist (Addgene plasmid # 14244 ...
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bioRxiv - Biochemistry 2020Quote: pRK5-HA-GβL was obtained from Addgene (1865). His-SUMO1 ...
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bioRxiv - Systems Biology 2020Quote: ... BioID2 in pcDNA3.1-BioID2-HA plasmid (Addgene, #74224) was PCR amplified with primers containing the FLAG epitope for N- or C-terminus integration (namely ...
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bioRxiv - Cancer Biology 2022Quote: ... 901 pLNCX myr HA Akt1 (Addgene, Cat #9005) was used for transient overexpression of active AKT ...
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bioRxiv - Cell Biology 2019Quote: ... HA-ARHGAP29 was purchased from Addgene (Plasmid #104154). cDNAs for STXBP4 and HOMER1 ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... pRK5-HA GST RagA_Q66L (RagAGTP; Addgene plasmid # 19300), pRK5-HA GST RagB T54L (RagBGDP ...
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bioRxiv - Biochemistry 2021Quote: ... HA tagged USP5 (#22590) was purchased from Addgene and subcloned into pcDNA5 mammalian expression vector ...
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bioRxiv - Neuroscience 2021Quote: ... pme-HA-NLS-tdMCP-tagRFP (Addgene Plasmid 86244) (Campbell et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Akt-HA-pcDNA 3.1 was precured from Addgene. For yeast two hybrid analysis ...
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bioRxiv - Genomics 2021Quote: ... amplified from pICE-HA-MRE11-WT (Addgene, 82033) using primer pairs oJL162 and oJL163 (Table S7) ...
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bioRxiv - Cancer Biology 2019Quote: ... pCMV-HA-KDM4B was obtained from Addgene (24181). The catalytic domain of KDM4B(1-348 ...
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bioRxiv - Cell Biology 2022Quote: ... together with HA-tagged Raptor (Plasmid #8513, Addgene). 36 h post-transfection ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... and pRK5-HA-Ubiquitin-K48 (Addgene plasmid #17605) were a kind gift from Ted Dawson and described previously (54).
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bioRxiv - Cancer Biology 2023Quote: ... and HA-NLS-PTEN were procured from Addgene. NLS-CHIP was generated by replacing EBFP from pEBFP-nuc (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... and pcDNA3/hArf6(Q67L)-HA (Addgene plasmid # 79425) (the plasmids were a gift from Kazuhisa Nakayama (Makyio et al ...
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bioRxiv - Cancer Biology 2023Quote: ... we used HA-VHL-pRC/CMV (Addgene #19999) and HA-tagged mouse VHL ORF Clone (Origene ...
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bioRxiv - Cell Biology 2023Quote: ... pBMN-HA-NAP1 delta-TBK1 (L226Q/L233Q) (RRID:Addgene_208869), pBMN-HA-SINTBAD (RRID:Addgene_) ...
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bioRxiv - Immunology 2023Quote: ... The HA-ubiquitin plasmid was obtained from Addgene (pRK5-HA-Ubiquitin-WT93 ...
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bioRxiv - Microbiology 2021Quote: ... by flow cytometry on HEK293-FT cells transiently expressing SARS-CoV-2 N (Addgene # 141391), S (BEI # NR-52310 ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells expressing transiently-transfected actin fused to monomeric azure-fluorescent protein (mAzure-Actin; Addgene) were grown on coverslips in 12-well plates at 2×105 cells/well and infected with Bp340 ...
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bioRxiv - Cell Biology 2020Quote: For derivation of HA-Smo and Smo-HA ESCs mouse Smo was amplified by PCR from the pGEN-mSmo (Addgene, #37673) and introduced in the PB-HA-IRES-Neo vector ...
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bioRxiv - Molecular Biology 2023Quote: ... from the Syn promoter (pLV-FLAG-HA_AGO2) was generated by amplifying FLAG/HA-AGO2 from pIRESneo-FLAG/HA AGO2 (Addgene plasmid 10822).
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bioRxiv - Genetics 2019Quote: Plasmid pF2-bio-His was obtained from Addgene (plasmid #52179) and human F2 cDNA was amplified using primers containing vector sequence homology (Table S1) ...
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bioRxiv - Cell Biology 2019Quote: His-tagged ubiquitin construct was obtained from Addgene (plasmid: #18712). siRNA#1 resistant RNF168-mCherry plasmid and RNF168 siRNAs were gift from Jiri Lucas (1) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163) and SARS-CoV-2 nsp14/nsp10-His-3xFlag (Addgene ID 169164 ...
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bioRxiv - Biochemistry 2021Quote: Plasmids SARS-CoV-2 nsp10-His-3xFlag (Addgene ID 169162), SARS-CoV-2 3xFlag-His-nsp5CS-nsp14 (Addgene ID 169163 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into a His-SUMO backbone (Addgene Plasmid #37507) for expression ...
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bioRxiv - Biochemistry 2022Quote: ... The acp ORF was amplified from pET29a-acp-His (Addgene) using primers listed in Table S5 and inserted into SacI- and BamH1-digested pET28b-Smt3-His10 to give pET28b-Smt3-His10-acp.
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bioRxiv - Molecular Biology 2020Quote: ... pET-His-hRan-Q69L was acquired from AddGene (catalog #42048) and pGEX-TEV-hCRM1 (XPO1 ...
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bioRxiv - Bioengineering 2023Quote: ... were transformed with the plasmid pET28b-RfxCas13d-His (Addgene 141322), which contain the recombinant CasRx gene ...
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bioRxiv - Genomics 2023Quote: ... were transformed with pET-28b-Cas9-His (Addgene, no. 47327) by heat shock following the manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: His-SIRT5 expression plasmid was obtained from Addgene (plasmid #25487). Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518 ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected at 4-6 h with a plasmid encoding HA-Separase (pCS2+HA-hSeparase was a gift from Marc Kirschner, Addgene plasmid # 33018), Plk1TD expression was induced 8-10 h after shake off and cells were fixed at 28 h to analyze distancing or at 32 h (20 h of S phase arrest ...
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bioRxiv - Cancer Biology 2021Quote: ... a gBlock (IDT) containing HA fused to FRB* was subcloned into the AgeI and KpnI sites of MYOF-HA (Addgene plasmid, #22443) to generate MYOFΔTM-HA-FRB* (referred to as MYOF-FRB*) ...
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bioRxiv - Immunology 2022Quote: ... pTRIPZ-puro-HA-Ub was generated by Gibson assembly by PCR amplification of HA-tagged ubiquitin gene from the plasmid HA-Ubiquitin which was a gift from Edward Yeh (Addgene plasmid # 18712) and pTRIPZ-puro digested with AgeI and XhoI ...
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bioRxiv - Systems Biology 2021Quote: ... was amplified from plasmid ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC (Wu et al., 2014) (gift from Robert Singer; Addgene plasmid #52985). The IRES-SV40/NLS-MCP gene sequence together with full-length mVenus gene amplified from pTriEx-NTOM20-mVenus-Zdk2 (Wang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and Rcc1-HA (Addgene #106938, gift from Patrizia Lavia) were transfected using TransIT-2020 24 hour before immunostaining.
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bioRxiv - Cell Biology 2020Quote: ... HA-ubiquitin pcDNA3 expression plasmid was purchased from Addgene.
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bioRxiv - Cancer Biology 2019Quote: The pcDNA3.1 MCS-BirA(R118G)-HA (Addgene plasmid #36047) were kindly provided by Kyle Roux 33 ...
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bioRxiv - Biochemistry 2019Quote: ... Flag-HA-UCHL1 construct was obtained from Addgene (22563). Catalytically inactive mutant (C90A ...
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bioRxiv - Cell Biology 2019Quote: ... we used pHAGE EF1alpha dCAS9-VP64-HA (Addgene, #50918). Two gRNA sequences targeting different regions of linc00899 (guide 1 and 2 ...
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bioRxiv - Cell Biology 2021Quote: ... HA Tat and Flag NQO1 were purchased from Addgene. HA Mdm2 was purchased from Sino Biologicals ...
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bioRxiv - Molecular Biology 2020Quote: ... pIRESneo-FLAG/HA AGO1 (Addgene plasmid # 10820, FH-AGO1) and pIRESneo-FLAG/HA EYFP (Addgene plasmid # 10825 ...
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bioRxiv - Neuroscience 2020Quote: ... HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712 ...