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Citations for Addgene :
101 - 150 of 1501 citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Constitutively active androgen receptor (EGFP-C1-AR-V7)26 was obtained from Michael Mancini and Marco Marcelli (Addgene #86856). A plasmid containing estrogen receptor alpha (pEGFP-C1-ERα ...
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bioRxiv - Neuroscience 2023Quote: ... A control vector lacking the hM4Di receptor was also used (AAV8-CaMKII-EGFP; 2.1 x 1013 gc/ml Addgene viral prep # 50469-AAV8 ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Cancer Biology 2024Quote: ... the mApple-Alpha-V-Integrin-25 was a gift from Michael Davidson (Addgene plasmid # 54866; http://n2t.net/addgene:54866; RRID: Addgene_54866), the pCX-EGFP beta5 integrin receptor 20 was a gift from Raymond Birge (Addgene plasmid # 14996 ...
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bioRxiv - Neuroscience 2022Quote: ... rats received injections of AAV5.CMV.HI.eGFP-Cre.WPRE.SV40 (Addgene #105545, 0.3μl). In the Pf (females ...
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bioRxiv - Biochemistry 2022Quote: ... Yi Zhang (Addgene plasmids # 60941 and 60942, http://n2t.net/addgene:60941, RRID:Addgene_60941, and http://n2t.net/addgene:60942, RRID:Addgene_60942 [35]). The DNA oligonucleotides used in this work are summarized in Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... were infused bilaterally with AAV expressing the inhibitory designer receptor hM4Di (AAV8-hSyn-hM4Di-mCherry, 4.8 × 1012 or 3.7 × 1012 vg/mL; Addgene; 0.4 μL) at a rate of 0.1 μL/min into the BLA (AP ...
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bioRxiv - Neuroscience 2019Quote: ... CA) or the excitatory DREADD receptor-encoding virus (AAV5-hSyn-DIO-hM3Dq-mCherry, 6.50E+12 GC/mL, Addgene, Watertown, MA). Viral incubation occurred for at least 8 weeks during the post-surgical recovery ...
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bioRxiv - Neuroscience 2023Quote: ... striatal ChI firing rates were compared between groups of ChAT-Cre positive mice injected with either AAV8-hSyn-DIO-hM3d(Gq)-mCherry (ChIs would express mCherry and the Gq receptor and be excited by CNO) or injected with AAV8-hSyn-DIO-mCherry (Addgene Catalog # 50459-AAV8 ...
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bioRxiv - Neuroscience 2023Quote: ... an excitatory designer drug acting exclusively on designer receptors (eDREADDs) in MCs was AAV2-hSyn-DIO-hM3D(Gq)-mCherry (#44361, Addgene). Controls were injected with AAV5-EF1a-DIO-mCherry (UNC Vector Core) ...
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bioRxiv - Microbiology 2020Quote: The integrins subunits were overexpressed in CHO cells line with the following plasmids: Alpha 5 integrin-GFP (Addgene plasmid# 15238)50 ...
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bioRxiv - Microbiology 2022Quote: ... A repair template encoding TIR1 under the control of the alpha tubulin promoter (pTUB1) and a CAT expression cassette was amplified from Addgene plasmid #87258 using oligos P1/P2 ...
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bioRxiv - Cancer Biology 2019Quote: ... sequence (5’-TGAAAGCCACCAGACCTCGA) targeting exon 8 of the murine leptin receptor (LepR) was ligated into the BsmBI site in lentiGuide-Puro (Addgene 52963) with compatible annealed oligos to generate lentiGuide-Puro-sgLepR ...
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bioRxiv - Bioengineering 2020Quote: ... The ScFv library with the N-terminal CD8α signal peptide was fused to the synNotch-Gal4VP64 receptor backbone (Addgene plasmid #79125) in place of the CD19-specific scFv ...
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bioRxiv - Molecular Biology 2020Quote: ... Immortalized Smarca4AID/AID preadipocytes were infected with retroviral vector expressing the auxin receptor Tir1 of rice (pBabePuro-OsTir1-9Myc, Addgene #80074). To induce degradation of SMARCA4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Enhanced serotype inhibitory Designer Receptors Exclusively Activated by Designer Drugs (DREADD) vectors used were AAV-PHP.eB-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362, ME) (Chan et al. ...
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bioRxiv - Cell Biology 2023Quote: The DNA fragment encoding human integrin β5 was amplified from the pCX-EGFP beta5 integrin receptor (a gift from Raymond Birge, Addgene #14996), and then inserted into the pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... sapiens beta-2-adrenergic receptor used as a control in BRET2 experiments was a gift from Robert Lefkowitz (Addgene plasmid #14697). Mm Arr-2 (NP_796205.1 ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned CreERT2 (Cre recombinase fused to a mutant ligand-binding domain of the estrogen receptor) and IRES into the BamHI site of pAAV-EF1a-tdTomato-WPRE-pGHpA (Addgene #67527). To prevent leakage of Cre activity34 ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frames of the HAs of A/Vietnam/1203/2004 H5 (Addgene plasmid #182546, [35]), A/Puerto-Rico/8/1934 H1 [39] ...
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bioRxiv - Cell Biology 2020Quote: Mammalian expression plasmids encoding rat LAMP1 (mCherry-Lysosomes-20, Addgene; 55073), mApple-LAMP1-pHluorin (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles for cas9 and HSP90-alpha were produced using HEK293T cells by con-transfection of transgene plasmid and pMD2.G (Addgene# 12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2022Quote: Single guide RNA sequences targeting murine Lrp1 were obtained from the GeCKO v2 library (35) and cloned into the pSpCas(BB)-2A-Puro (PX459) V2.0 vector (RRID:Addgene_62988). B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-PTRE-tight-hM3Dg-mCherry(35) (a gift from William Wisden [Addgene plasmid # 66795; http://n2t.net/addgene:66795; RRID:Addgene_66795]). Specifically ...
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bioRxiv - Plant Biology 2024Quote: 35S:GUS-pMAS:GFP was modified by replacing the 35S promoter with the MAS promoter from pGFPGUSPlus (Plasmid #64401, Addgene) (He et al. ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs: 12251, 12253, 12259) (67) ...
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bioRxiv - Cancer Biology 2022Quote: ... The constitutively active PKC mutant expression plasmid (PKC alpha CAT) was a gift from Bernard Weinstein (Addgene plasmid # 21234; http://n2t.net/Addgene:21234; RRID: Addgene_21234). YAP/TAZ-responsive synthetic promoter (8xGTIIC-luciferase ...
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bioRxiv - Neuroscience 2022Quote: ... ChAT-Cre or WT rats were injected with pAAV5-hSyn-mCherry (Addgene) at a titer of 1.4-2.8×1013 GC/mL.
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bioRxiv - Biophysics 2022Quote: The plasmid encoding GPCR kinase subtype 2 used for the receptor phosphorylation in insect cells (GRK2-CAAX) was a gift from Robert Lefkowitz (Addgene plasmid #166224 49). Full-length arrestin21-418 (C150L ...
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bioRxiv - Cell Biology 2019Quote: Human GeCKOv2 CRISPR knockout pooled library (Pooled Library #1000000048)35 and pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230)97 were gifts from Feng Zhang ...
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bioRxiv - Synthetic Biology 2021Quote: The pEditor fragment of AID_T7_UGI was amplified from the vector pcDNA3.1 (+)- Hyperactive AID-T7 RNA Polymerase-UGI-T2A-td Tamato (Addgene 138610; [35]) using primers SphI_AID_T7_F and NSiI_XbaI_AID_T7_R ...
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bioRxiv - Plant Biology 2023Quote: ... and rbcS-E9 enhancers and their 169-bp 3′ and 5′ segments as well as the 35S enhancer were PCR amplified from pZS*11_4enh (Addgene no ...
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bioRxiv - Neuroscience 2022Quote: ... Rats in control groups received either rAAV5/hSyn-DIO-mCherry (Addgene #50459, 0.75μl), saline (1.2μl ...
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bioRxiv - Cancer Biology 2024Quote: ... DIV6 rat cortical neurons were treated with AAV-mDlx-NLS-mRuby (Addgene #99130) at a titer of >1x109 vg/ml.121 AAV-mDlx-NLS-mRuby2 was a gift from Viviana Gradinaru (Addgene plasmid #99130 ...
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bioRxiv - Plant Biology 2020Quote: The human codon-optimized CAS9 with 2×35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using KpnI forward and PacI reverse primers (Supplemental Table 9) ...
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bioRxiv - Microbiology 2020Quote: ... The sfgfp gene was PCR-amplified with primers 35 and 36 from plasmid pHR-scFv-GCN4-sfGFP-GB1-NLS-dwPRE (gift from Ron Vale; Addgene plasmid # 60906 ...
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bioRxiv - Plant Biology 2023Quote: ... The module for selection was obtained from pHDE-35S-Cas9-mCherry-UBQ which was a gift from Yunde Zhao (Addgene plasmid # 78932 ...
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bioRxiv - Cell Biology 2023Quote: ... 2-6×105 cells in a 35 mM well were transfected with 0.4ug of DNA encoding a puro resistance plasmid (pCoPURO, Addgene #17533) using Effectene Transfection Reagent (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
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bioRxiv - Neuroscience 2020Quote: ... a transfer plasmid containing rat Synapsin promoter and cDNA encoding GCaMP6s (Addgene plasmid #40753) was assembled and transfected with helper-free DJ plasmids (Cell Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... ChAT-Cre or WT rats were injected with pAAV5-hSyn-hM3D(Gq)-mCherry (Addgene) at a titer of 1.5×1013 GC/mL ...
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bioRxiv - Neuroscience 2022Quote: ... ChAT-Cre rats were injected with pAAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, USA) at a titer of 7.8-8.2×1012 genome copies (GC)/mL ...
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bioRxiv - Neuroscience 2023Quote: ... rats received infusions of 500 nL of AAV5-hsyn-ChR2-eYFP (n= 22; Addgene 26973 ...
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bioRxiv - Plant Biology 2019Quote: ... ETT-FLAG was generated using Golden Gate cloning39 by recombining a previously described L0 clone for ETT with a 35S promoter (AddGene #50266), a C-terminal 3xFLAG epitope (AddGene #50308 ...
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bioRxiv - Developmental Biology 2022Quote: ... 35 μl of hot glycerol was cooled to 4°C then 35 μl of the primer mixture and 25 μl of Tn5 (Addgene #112112) was added and mixed and held at 1 hr at RT with gentle pipet mixing every 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... we first amplified the cassettes including sgRNA using the primer set priEK-35 and priEK-37 from the CRISPRi-V2 library (Addgene #1000000093) using Phusion polymerase (New England Biolab ...
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bioRxiv - Genetics 2020Quote: The LexAop-LacZ plasmid was prepared by cloning the BglII-LexAop-hsp70-HindIII(filled) fragment of pJFRC18-8XLexAop2-mCD8:GFP [35] (Addgene plasmid #26225) into the BamHI/EcoRI(filled ...
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bioRxiv - Biochemistry 2020Quote: ... without start codons and without their native signal peptides identified using SignalP 5.0 with the Gram-positive setting.35 Their open-reading frames were cloned into the expression vector p15TV-L (AddGene ID: 26093) under the T7 promoter and in-frame with the N-terminal 6xHisTag (Twist Bioscience) ...
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bioRxiv - Developmental Biology 2019Quote: ... sense and antisense oligos with overhangs fitting the sticky ends of the BbsI digested vector were annealed and ligated into pU6-BbsI-chiRNA plasmid (a gift from Melissa Harrison & Kate O’Connor-Giles & Jill Wildonger, Addgene plasmid # 45946 [35]). Site 1 (ctgctcaggtgagtccg/gga ...
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bioRxiv - Neuroscience 2021Quote: Rats underwent stereotaxic surgery to inject either an excitatory (AAV8.hSyn.hM3Dq.mCherry; Addgene, catalog # 50474-AAV8) or inhibitory (AAV8.hSyn.hM4Di.mCherry ...