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Citations for Addgene :
401 - 450 of 982 citations for Mouse anti Ebola Virus envelope glycoprotein GP Zaire M921 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: pAAV.Syn.GCaMP6f.WPRE.SV40 virus (titer: 2.2 × 1013 GC/mL, obtained from AddGene, USA) was injected into dorsal hippocampus (AP -2.1 ...
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bioRxiv - Neuroscience 2021Quote: The adenoassociated virus (AAV) pAAV2/9.CAG.FLEX.GCaMP6s.WPRE.SV40 was purchased from Addgene (Addgene viral prep #100842-AAV9 ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 μL AAV pCAG-FLEX-EGFP-WPRE virus (Addgene, Cambridge, MA) was injected into the NTS ...
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bioRxiv - Immunology 2020Quote: ... and a murine leukemia virus (MLV) Gag-Pol plasmid (Addgene # 14887) were purified using the Endo-Free Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The AAV-hSyn-DIO-GCaMP7f-WPRE (AAV9 virus) are from Addgene.
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bioRxiv - Neuroscience 2022Quote: ... the adeno-associated virus AAV1.CAG.FLEX.tdTomato.WPRE.bGH (#59462, Addgene, Watertown, MA, USA) was used ...
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bioRxiv - Neuroscience 2023Quote: ... Virus (AAV1.syn.jGCaMP7s.WPRE.SV40 from Addgene, lot v50167, titer 2.7E13 GC/mL) was then injected at 2 sites within the durotomy ...
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bioRxiv - Neuroscience 2023Quote: ... WPRE.SV40 virus (titer: 4 × 1013 GC per ml, obtained from Addgene) was lowered to a depth of 2.4 mm below the dura ...
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bioRxiv - Cell Biology 2023Quote: ... U2OS cells were transduced with virus containing lentiCas9-Blast (Addgene, #52962). Cells were then treated with 5 µg/mL blasticidin for 5 days to make a stable population of U2OS-Cas9 cells ...
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bioRxiv - Neuroscience 2023Quote: ... or control virus AAV-hSyn-EYFP (AddGene, 50465-AAV2, Watertown MA) into the CeA (−1.17 mm AP ...
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bioRxiv - Neuroscience 2023Quote: ... and a cre-dependent virus (AAV9-DIO-Ef1a-eYFP, Addgene #27056) were used.
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bioRxiv - Cell Biology 2023Quote: ... transduced with recombinant adeno-associated virus (rAAV) (Addgene, pAAV TBG FFLuc) supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... 2.5 mm V) and retrograde AAV-Syn-jGCaMP8f-WPRE virus (Addgene) was injected using a Nanoject ...
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bioRxiv - Neuroscience 2023Quote: ... retroAAV-CAG-GFP (virus made in the host institute, using Addgene plasmid #37825 ...
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bioRxiv - Neuroscience 2023Quote: ... Cre or GFP expressing adeno-associated virus (GFP pAAV.CMV.PI.EGFP.WPRE.bGH, Addgene 105530; Cre pENN.AAV.CMVs.PI.Cre.rBG, Addgene 105537) were bilaterally injected into the mPFC of AT2R-flox mice at 2.5mm caudal ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transduced with virus expressing dCas9-VP64 (Addgene, Watertown, MA), selected using blasticidin (6 – 10 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... was produced in HEK293T cells that were co-transfected with the vector containing the gene of interest and second generation envelope and packaging plasmids (psPAX–gift from Didier Trono (Addgene plasmid#12260 ...
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bioRxiv - Cell Biology 2022Quote: Lentiviruses were produced by MaxPEI-based co-transfection of HEK293T cells with the transfer vectors together with the packaging vector psPAX2 and envelope vector pMD2.G (psPAX2 and pMD2.G were a gift from Didier Trono, Addgene plasmid #12259 and #12260 ...
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bioRxiv - Bioengineering 2019Quote: Genetic engineering of the reporter gene system involved third-generation packaging and envelope-expression plasmids (pMDLg/pRRE, pRSV-Rev, and pMD2.G, Addgene plasmids ...
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bioRxiv - Cell Biology 2021Quote: The lentiviral packaging plasmid psPAX2 and the VSV-G envelope plasmid pMD2.G were gifts from Didier Trono (psPAX2: Addgene plasmid #12260 ...
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bioRxiv - Neuroscience 2020Quote: ... or shRNA constructs in combination with the psPAX2 packaging and pCMV-VSV-G envelope plasmids (Addgene plasmid #12260 and #8454) using FuGene HD (Promega ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were transfected with the pLenti-VIM plasmid in combinations with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G (Addgene) using TransIT_293 transfection reagent (Mirus) ...
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bioRxiv - Microbiology 2020Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Cell Biology 2022Quote: ... tat (transcriptional regulator) and rev (post-transcriptional regulator) and the envelope plasmid psPAX2 encoding VSV-G coat protein were purchased from Addgene repository ...
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bioRxiv - Immunology 2022Quote: ... or the scrambled shRNA sequence: CCGGCA-ACAAGATGAAGAGCACCAATTTTT were transfected overnight into 293 LentiX packaging cells (Takoro) along with VSV-G plasmid envelope (Addgene), aiming to generate shLRBA and shControl encoding lentiviruses ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259 ...
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bioRxiv - Systems Biology 2022Quote: ... cells were transfected with 750ng of an equimolar mixture of the three third-generation envelope and packaging plasmids (pMD2.G: Addgene #12259; pRSV-Rev: Addgene #12253 ...
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bioRxiv - Biochemistry 2023Quote: ... 8 µg psPAX2 packaging plasmid DNA and 1 µg pMD2.G envelope plasmid DNA (both gifts from Didier Trono, Addgene plasmid no ...
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bioRxiv - Cancer Biology 2023Quote: ... These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr, addgene#8455) and envelope plasmid (pCMV-VSV-G, Addgene#8454) were used for lipofectamine 2000 mediated transfection of 293FT cells to produce lentiviral pseudo typed particles ...
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bioRxiv - Cell Biology 2023Quote: ... at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G: Addgene 12259 ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant DNA constructs cloned in lentiviral plasmids described above were co-transfected with packaging and envelope plasmids psPAX2 (gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Microbiology 2023Quote: ... semi-confluent HEK 293T cells in 10cm cell culture grade petri dishes were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Molecular Biology 2024Quote: ... ORFs of interest were overexpressed using second-generation lentiviral system that consisted of pCMV-VSV-G (Weinberg lab) envelope plasmid and PsPAX2 (Trono lab) packaging plasmid acquired from Addgene. HEK293t cells were transfected with plasmid DNA at a ratio of 5:4:1 transfer plasmid:envelope plasmid:packaging plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were generated by transfecting HEK 293T cells with 12 μg of pLJM1-mEGFP or pLJM1ALKBH5 plasmids with 6 μg of psPAX2 packaging plasmid and 2 μg of pMD2.G envelope plasmid (Addgene). The medium was replaced 16h after transfection ...
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bioRxiv - Genomics 2024Quote: ... Lentivirus was produced in HEK293T cells co-expressing the shRNA plasmid together with psPAX2 packaging plasmid and pVSV-G envelope plasmid (Addgene). Virus was concentrated using Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2024Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Cell Biology 2023Quote: ... to co- transfect HEK 293FT cells with plasmids containing the packaging gag/pol and envelope pCMV- 435VSV-G genes and either the pMRX-IB-HaloTag7-mGFP-KDEL (Addgene, 184904 ...
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bioRxiv - Physiology 2021Quote: ... 100 U/ml penicillin and 100 ug/ml streptomycin in a 5% CO2/95% air atmosphere at 37C Plasmid GP-CMV-GCaMP6s (Addgene plasmid # 40753) was cloned into lentiviral vector pCDH-EF1-MCS-IRES (puro ...
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bioRxiv - Synthetic Biology 2022Quote: ... the InterCatch and InterTag sequences were cloned into pSBbi-GP and pSBbi-BP (Kowarz et al. [70]. Addgene #60511 and Addgene #60512) Sleeping Beauty cassettes respectively ...
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bioRxiv - Neuroscience 2022Quote: ... or control virus pAAV-CaMKIIa-EGFP (Addgene Cat# 50469-AAV5, titer: 4.3x1012) into the CA2 using the following coordinates from Bregma ...
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bioRxiv - Neuroscience 2021Quote: ... The control virus (AAV8-hSyn-DIO-mCherry, 2.3×1013 GC/mL, Addgene) was injected according to the same procedure ...
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bioRxiv - Biochemistry 2021Quote: Tobacco Etch Virus protease (TEV) was a gift from Helena Berglund (Addgene plasmid # 125194 ...
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bioRxiv - Neuroscience 2022Quote: ... a virus expressing GCaMP6s (AAV9.Syn.GCaMP6s.WPRE.SV40, ~1.9 × 1013 GC/ml; Addgene # 100843) was injected unilaterally in the A13 while for DREADD experiments viruses for reversibly activating (AVV8-Syn-hM3D-Gq-mCherry ...
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bioRxiv - Neuroscience 2019Quote: ... or AAV5-hDlx-GqDREADD-dTomato (3.15×10^15 virus molecules/mL) (Addgene plasmid # 83897 ...
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bioRxiv - Neuroscience 2019Quote: ... Virus (1012 titer; AAV2/2 camKII-KV2.1-ChrimsonR-FusionRed; Addgene, plasmid #102771) was injected 400 μm below the dura (4-10 sites ...
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bioRxiv - Neuroscience 2020Quote: ... 1.2 ul of AAV8-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene, USA) were bilaterally injected into 6 PV-Cre animals bilaterally ±0.5 mm lateral to midline ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5μl pGP-AAVrg-syn-jGCaMP7f-WPRE virus 25 (1.85× 1013GC/ml; Addgene) was infused into the NAc (A/P ...
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bioRxiv - Neuroscience 2022Quote: ... the virus used was an AAV5-FLOX-ChR2-mCherry (Addgene, #20297-AAV5) or a AAV5-Ef1a-DIO-eYFP (UNC Vector Core) ...
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bioRxiv - Immunology 2023Quote: ... Fresh virus of (MSCV-myc-CAR-2A-Thy1.1, Anjana Rao, Addgene: 127890) containing chimeric antigen receptor (CAR ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µl of AAV9.CAG.GCaMP6s.WPRE.SV40 or AAV9.syn.GCaMP8s-WPRE virus (Addgene, USA) was injected subcutaneously in the nape of the neck ...