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Citations for Addgene :
1 - 50 of 768 citations for Mouse Serine threonine protein kinase PINK1 mitochondrial PINK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... the PINK1 insert was derived from pCMVTNT-PINK1-C-Myc (Addgene, #13314) and the mNeonGreen insert was obtained as cDNA (Allele Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... pEYFP-N1-Pink1 was a gift from Richard Youle (Addgene plasmid # 101874 ...
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bioRxiv - Biochemistry 2023Quote: ... Pediculus humanus corporis PINK1 (115 – 575) was a gift from David Komander 34(Addgene plasmid # 110750). Ube1 was a gift from Cynthia Wolberger 35(Addgene plasmid # 34965).
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bioRxiv - Cell Biology 2023Quote: ... pEYFP-N1-Pink1 was a gift from Richard Youle (Addgene plasmid # 101874; http://n2t.net/addgene:101874; RRID:Addgene_101874). The plasmid pAG1345 for the expression of mCherry-FIREtag-DEVDG-MoA was constructed by inserting the sequence coding for DEVDG-MoA ...
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bioRxiv - Biochemistry 2019Quote: ... with Protein kinase A (M. musculus PKA catalytic subunit alpha; Addgene 14921) expressed with an N-terminal His tag ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondrial matrix APEX2 control vector (Addgene #72480) was also subcloned into pBABE-Puro ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial targeted mCherry was from Addgene (#55102). GFP-tagged human wild type tau (0N4R ...
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bioRxiv - Neuroscience 2021Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA) to drive expression preferentially in principal neurons ...
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bioRxiv - Neuroscience 2023Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA). Virus (0.5 µl ...
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bioRxiv - Physiology 2021Quote: ... we used mitochondrial marker CEPIA2-mt (Addgene plasmid, Cat #58218) as previously described (Kostic ...
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bioRxiv - Cell Biology 2021Quote: ... or 50 ng of a mitochondrial marker (eBFP2-Mito, Addgene: 55248). The next day ...
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bioRxiv - Cell Biology 2019Quote: ... and a plasmid encoding mCherry with a mitochondrial targeting sequence (RRID:Addgene_55102), were gifts from Michael Davidson ...
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bioRxiv - Systems Biology 2023Quote: ... we first digested mitochondrial matrix-localizing HyPer7 (pCS2+MLS-HyPer7, RRID:Addgene_136470) with BamH1 and XhoI and ligated into our lentiviral backbone (13) ...
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bioRxiv - Physiology 2023Quote: FLAG-IKK2 kinase inactive K44M (IKK2DN, Addgene, #15466) was cloned into pLenti-CMV-Puro DEST vector (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... mutation that converts threonine 154 into alanine) were PCR-amplified from pTRE-TIGHT-Cx43-eYFP (gifted from Robin Shaw; Addgene Plasmid #31807) and cloned into the pcDNA3.1-HA (gifted from Oskar Laur ...
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bioRxiv - Biochemistry 2019Quote: ... sapiens Pyk2 kinase domain expression vector PTK2BA encoding H6-TEV-kinase [414-692] was a gift from Nicola Burgess-Brown (Addgene # 42401). Cloning vector pET-H6-SUMO-TEV-LIC (1S ...
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bioRxiv - Molecular Biology 2022Quote: ... or pRK5-MYC-mTOR kinase-dead (Addgene plasmid #8482)) using the TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Cancer Biology 2023Quote: A human kinase domain-focused gRNA library (Addgene 117725) [25] was used to assess CRISPR Cas9 screening as a tool in breast cancer PDTO to identify whether novel vulnerabilities could be detected for each PDTO ...
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bioRxiv - Cancer Biology 2022Quote: ... and constitutively kinase-active (#64608) IKKα were purchased from Addgene. Briefly ...
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bioRxiv - Cell Biology 2021Quote: The genes encoding cytosolic and mitochondrial matrix-targeted roGFP (33) were recloned from Addgene vectors 49435 and 49437 into pcDNA5FRT/TO and transfected into Flp-In T-REx 293 cells (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...
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bioRxiv - Genomics 2021Quote: ... KRAB and MeCP2 were linked to dCas9 with flexible glycine-serine linkers and cloned into lentiCas9-Blast (Addgene 52962) (22).
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bioRxiv - Molecular Biology 2023Quote: Ro-GFP (reduced-oxidized GPP) plasmid targeted to mitochondrial matrix was purchased from Addgene (#49437) and delivered into cells by electroporation ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression vectors of the full-length SARS-CoV-2 spike and the human serine protease TMPRSS2 with a C-terminal C9-tag (TETSQVAPA) were acquired from AddGene (Summit Pharmaceutical International ...
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bioRxiv - Cell Biology 2020Quote: ... A negative selection cassette with human thymidine kinase was retrieved from Addgene #21911 (41) ...
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bioRxiv - Cell Biology 2020Quote: ... GST-Snf1 kinase domain (Snf1-KD) expression vector was purchased from Addgene (#52683). Those DNA constructs were transformed in Rosetta2 (Novagen ...
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bioRxiv - Immunology 2021Quote: ... Mito-PAGFP encoding for an eGFP with mitochondrial target sequence was a gift from Richard Youle (Addgene plasmid # 23348 ...
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bioRxiv - Cell Biology 2021Quote: ... and mitochondrial (CMV-mito-CAR-GECO1) targeted calcium biosensors were gifts from Robert Campbell (Addgene #61244, and #46022) 44 ...
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bioRxiv - Immunology 2021Quote: ... Mito-PAGFP encoding for an eGFP with mitochondrial target sequence was a gift from Richard Youle (Addgene plasmid # 23348; http://n2t.net/addgene:23348; RRID:Addgene_23348)65.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transfected with the mitochondrial calcium indicator pCMV CEPIA2mt (a gift from Masamitsu Iino; Addgene plasmid # 58218; http://n2t.net/addgene: 58218; PRID:Addgene_58218) (Suzuki et al. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transfected with the mitochondrial calcium indicator pCMV CEPIA2mt (a gift from Masamitsu Iino; Addgene plasmid # 58218 ...
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bioRxiv - Developmental Biology 2019Quote: ... was a gift from Elisa Izaurralde (Addgene plasmid # 37370) (78) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells stably expressing the Mito-DsRed mitochondrial probe were generated by infecting cells with pLV-mitoDsRed (Addgene, plasmid 44386) lentivirus particles with puromycin (2 μg/mL ...
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bioRxiv - Genomics 2023Quote: ... according to the manufacturer’s instructions to remove the 78 bp mitochondrial localization signal (MLS) from RNase H1 cDNA (AddGene; pFRT-TODestGFP_RNAseH1 ...
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bioRxiv - Cell Biology 2022Quote: Full-length ERK3 wild type (WT) pDONR-223 construct purified from Human Kinase Library (Addgene) was used as a template to generate ERK3 K49A K50A kinase dead (KD ...
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bioRxiv - Cell Biology 2023Quote: ... we expressed the ULK1 kinase from a pCAG backbone encoding MBP-TSF-TEV-ULK1 (RRID:Addgene_171416). The transfection and expression procedure was similar to what is described above for NAP1 ...
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bioRxiv - Cell Biology 2023Quote: ... the mitochondrial Mit-RFP probe22 and the Cox8-EGFP-mCherry mitophagy reporter (a gift from David Chan; Addgene plasmid # 78520)23 using jetPRIME (polyplus transfection ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments containing MAP3K1 (MEKK1) or kinase-dead MAP3K1 were excised from pCDNA-MEKK1 plasmids (Addgene #12181 and #12180 ...
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bioRxiv - Neuroscience 2021Quote: ... AAV-CAG-DIO-APEX2-MITO was generated in our lab by cloning the mitochondrial targeting sequencing from mito-V5-APEX2 (Addgene #72480) and placing it on the 5’ end of APEX2-NES in AAV-CAG-DIO-APEX2NES ...
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bioRxiv - Cell Biology 2020Quote: ... The tandem mitochondrial-targeted mCherry-eGFP mitophagy reporter plasmid (pCLBW-cox8-EGFP-mCherry) was a gift from David Chan (Addgene 78520). pLKO.1-shNT ...
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bioRxiv - Biochemistry 2021Quote: ... and pT7-EGFP-C1-HsDCP2 were gifts from Elisa Izaurralde (Addgene plasmid # 25030 ...
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bioRxiv - Cell Biology 2023Quote: ... pT7-EGFP-C1-HsDCP1a was a gift from Elisa Izaurralde (Addgene plasmid # 25030 ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells plated on polylysine-coated coverslips were transfected with cDNA (100 ng/40000 cells) coding for mitochondrial-targeted mCherry (mCherry-Mito-7, a gift from Michael Davidson (Addgene plasmid # 55102), (Olenych et al ...
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bioRxiv - Genetics 2023Quote: ... fused to the herpes simplex virus thymidine kinase-encoding gene (HSV-TK) from the pFA6a-HyTkAX vector (Addgene plasmid # 73898; http://n2t.net/addgene:73898; RRID:Addgene_73898) [80] ...
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bioRxiv - Microbiology 2024Quote: ... Wild-type Myc-MEKK3 (in pcDNA3.1 vector) was generated from a kinase-dead mutant (K391A, Addgene plasmid # 44157)(55 ...
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bioRxiv - Immunology 2020Quote: ... A plasmid library of sgRNAs targeting all protein coding genes in the mouse genome (Brie Knockout library, Addgene 73633) was packaged into lentivirus using HEK293T cells ...
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bioRxiv - Cell Biology 2020Quote: ... The mitochondrial (CMV-mitoCAR-GECO1) and endoplasmic reticulum (CMV-ER-LAR-GECO1) targeted calcium biosensors were gifts from Robert Campbell (Addgene #46022 and #61244)(37,38 ...
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bioRxiv - Cancer Biology 2022Quote: Retroviral pLPCX vectors expressing the mitochondrial or cytoplasmic form of Grx1-roGFP227 and roGFP2-ORP123 were obtained from Addgene (Addgene, Watertwon, CA, USA) and used to transduce FL5.12MycER cells ...
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bioRxiv - Molecular Biology 2021Quote: ... pAc5.1C-FLuc-Stop-5BoxB was a gift from Elisa Izaurralde (Addgene plasmid # 21301)30 ...
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bioRxiv - Biochemistry 2019Quote: ... and a mammalian expression vector pcDNA3 encoding hemagglutinin tagged constitutively active glycogen synthase kinase-3β (GSK-3β) (S9A) (pcDNA3-GSK3β, # 14754, RRID: Addgene_14754). The EGFP in pRK5 vector was first replaced by iRFP to construct an iRFP tagged WT tau by golden gate assembly (pRK5-iRFP-tau ...