Labshake search
Citations for Addgene :
251 - 300 of 1885 citations for Mouse IgG2b Isotype Control Antibody Biotin A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... RRID:Addgene_20297) or eYFP alone as a control (AAV-EF1a-DIO-eYFP; serotype 5; Dr. Karl Deisseroth; RRID:Addgene_27056).
-
bioRxiv - Neuroscience 2024Quote: ... Cre-dependent control plasmid pAAV-hSyn-DIO-mCherry (AddGene #50459-AAV8; titer of 2.2×1013 GC/mL), or the retrogradely transported Cre-expressing plasmid pENN-rAAV-hSyn-HI-eGFP-Cre-WPRE-SV40 #105540-AAVrg ...
-
bioRxiv - Microbiology 2024Quote: ... Lentiviral vectors encoding control or TRIM5α miRNAs (pAPM-D4-miR30-L1221 or pAPM-D4-miR30-TRIM5α; Addgene) were produced by co-transfection with the packaging plasmid pcHelp (Mochizuki et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... Gata4 and Tbx5 (MGT) or pBabe-puro (an emptyback bone vector, used as mock/control; Addgene, #1784) were mixed with 4 μg pCL-Ampho (retroviral packaging plasmid) ...
-
bioRxiv - Cancer Biology 2020Quote: ... we designed single guide RNAs specifically targeting exon 1 of the mouse Bmal1 gene and subcloned it into the PX459 vector (Addgene) to obtain the PX459-Bmal1 plasmid ...
-
bioRxiv - Neuroscience 2020Quote: Full-length Susd4 mouse gene was cloned into the mammalian expression vector pEGFP-N1 (Addgene, Massachusetts, USA, Cat#6085-1) to express a SUSD4-GFP fusion construct under the control of the CMV promoter (pSUSD4-GFP) ...
-
bioRxiv - Developmental Biology 2020Quote: ... a FLAG-tag version of the codon-optimized mouse DUX was amplified by PCR (Primers in Extended Table 1) from pCW57.1-mDUX-CA (Addgene 99284) and subcloned into the pBS31 plasmid (pBS31-FLAG_mDUX) ...
-
bioRxiv - Cancer Biology 2021Quote: ... B16F10 pC and PANO2 pC were stably transfected with control (empty) overexpression vector eGFP.retro.puro (Addgene ID 64336, RRID:Addgene_64336) (3) ...
-
bioRxiv - Neuroscience 2022Quote: AAV expressing Cre-recombinase under the control of the CMV promoter (gift from Fred Gage; Addgene plasmid #49056).
-
bioRxiv - Neuroscience 2019Quote: For control conditions we used a plasmid containing EGFP under the CAG promoter (pCAG-EGFP, Addgene plasmid #11150) (Matsuda and Cepko ...
-
bioRxiv - Genetics 2019Quote: ... Cells were co-transfected with luciferase reporter plasmids (Figure 2A) or a positive control (pAP1-3, Addgene 71258) and a renilla normalisation control (pGL4.74 ...
-
bioRxiv - Cell Biology 2020Quote: Control plasmid encoding Sec61β-APEX2 (https://www.addgene.org/83411) was obtained from Addgene (S. Y. Lee et al., 2016) and APEX2-KDEL was generated by adding KDEL-encoding gene sequence to the 3’ reverse primer ...
-
bioRxiv - Neuroscience 2021Quote: ... Control (DATIREScre) neurons and α-syn overexpressing neurons (DATIREScre/α-syn) were transduced with AAV1-LoxP-tdTomato (Addgene) on DIV5 ...
-
bioRxiv - Neuroscience 2021Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA) to drive expression preferentially in principal neurons ...
-
bioRxiv - Cancer Biology 2021Quote: ... Transfection was performed with control vector (pcDNA/GW-40/LacZ) or 4 μg pARID1A (Addgene catalogue number 39311) using FuGENE 6 Transfection Reagent (Promega ...
-
bioRxiv - Neuroscience 2020Quote: ... Scramble shRNA was used for control electroporations and was a gift from David Sabatini (Addgene plasmid # 1864; http://n2t.net/addgene:1864; RRID:Addgene_1864). Raptor_1 shRNA was a gift from David Sabatini (Addgene plasmid # 1857 ...
-
bioRxiv - Cell Biology 2020Quote: ... Vector control cells were created by transfecting C2C12 cells with an empty pBABE-puro vector backbone (#1764, Addgene) and selected and kept in culture medium with 10 μg/mL puromycin.
-
bioRxiv - Molecular Biology 2022Quote: ... or a control shRNA (a gift from David Sabatini; Addgene plasmid #1864; http://n2t.net/addgene:1864; RRID: Addgene_1864) (52 ...
-
bioRxiv - Microbiology 2022Quote: ... expressing 76,441 sgRNAs against 19,114 human genes + 1,000 non-targeting sgRNA controls in plentiCRISPRv2 was obtained from Addgene (51). The library was electroporated into Endura electrocompetent cells (# 60242 ...
-
bioRxiv - Neuroscience 2022Quote: ... rAAV2/9 encoding jRGECO1a under control of the human synapsin promoter (4 × 1013 gc/ml; customized from AddGene plasmid #61563 ...
-
bioRxiv - Systems Biology 2023Quote: ... The control was Eahy926 cells transfected with a control plasmid (a gift from David Sabatini (Addgene plasmid #1864; http://n2t.net/addgene:1864; RRID:Addgene_1864)) (48) ...
-
bioRxiv - Neuroscience 2023Quote: ... AAV-rg/Cre was used to delete PTEN controls received AAV-rg/GFP (Addgene viral prep #37825-AAVrg). In study 4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The linear transcript used as a negative control consists of GFP expressed from pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid 12252).
-
bioRxiv - Cell Biology 2023Quote: ... The control construct delCMV-mCitrine was generated by ligating the larger fragment from pmCitrine-N1 (Addgene Plasmid #54594) with the smaller fragment from the delCMV-mCitrine-RBD biosensor (Graessl et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... The test wells were transfected with pHBx and control was transfected with GFP expressing plasmid (Plasmid #27082, Addgene). After three days post transfection transfected cells were treated with low dose puromycin (0.5 μg/ml ...
-
Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Transfection control contained 5% pEGFP puro (a gift from Michael McVoy, Addgene plasmid #45561(Abbate et al. 2001)) ...
-
bioRxiv - Neuroscience 2023Quote: ... expressing the genetically encoded calcium indicator GCaMP6f under control of the calcium/calmodulin-dependent protein kinase (CaMKII) promoter (pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40, Addgene, Watertown, MA). Virus (0.5 µl ...
-
bioRxiv - Neuroscience 2024Quote: The construct utilized for a transfection control comprised of an overexpression of methionine-tRNA synthetase (MeTRS) (AddGene, pMarsL274G) were created and sequenced using protocol described above.
-
bioRxiv - Cell Biology 2024Quote: Plasmids for mammalian expression of emIRFP670 and mIRFP713 under the control of CMV promoter were obtained from Addgene vectors #136556 and #136559 ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids expressing murine PKA (mPKA) RIIα and ER membrane targeting signal fused miniTurbo promiscuous biotin ligase (ER-mTb) were obtained from Addgene (#45527 and #107174, respectively). The mPKA RIIα sequence was PCR amplified and cloned into the upstream of the mTb sequence ...
-
bioRxiv - Neuroscience 2019Quote: ... recombinant mouse E1 (Addgene plasmid # 32534), E2 (pGEX4T-1-UbcH5b) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Mouse Ddx5 WT(Addgene plasmid #88869) and Lys144 to Asn mutant(Addgene plasmid #88870 ...
-
bioRxiv - Neuroscience 2022Quote: ... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
-
bioRxiv - Neuroscience 2023Quote: ... pCDNA3-mouse PKA-RIalpha-mEGFP (Addgene plasmid # 45525 ...
-
bioRxiv - Neuroscience 2023Quote: ... pcDNA3-mouse PKA-RIbeta-mEGFP (Addgene plasmid # 45526 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and firefly luciferase control (FLuc) were generated by subcloning FLuc-5Xbox b from plasmid pAc5.1C-Fluc-STOP-5boxb (Addgene) into pcDNA3.1(+ ...
-
bioRxiv - Cancer Biology 2019Quote: ... Noxa and a negative control non-targeting protospacer were individually cloned into BstXI/BlpI-digested pCRISPRia-v2 (Addgene #8432) by ligating annealed complementary synthetic oligonucleotide pairs ...
-
bioRxiv - Genetics 2019Quote: A self-complementary rAAV vector expressing GFP under control of a CAG promoter (pscAAV-CAG-GFP, Addgene, Cat#83279) was generated by replacing the CMV promoter in plasmid pscAAV-GFP (gift from John T Gray ...
-
bioRxiv - Biophysics 2021Quote: Monomeric and multimeric fluorescent controls were derived by modifying previously published plasmids designed to express CD86-EGFP (Addgene # 133858) and mApple-CD86-EGFP (Addgene #133860 ...
-
bioRxiv - Systems Biology 2021Quote: ... additional control plasmids (PA-NL, Addgene #113445; PA-mCit-NL, Addgene #113444; PA-mCit, Addgene #113443; NL, Addgene #113442) were used ...
-
bioRxiv - Systems Biology 2021Quote: ... additional control plasmids (PA-NL, Addgene #113445; PA-mCit-NL, Addgene #113444; PA-mCit, Addgene #113443; NL, Addgene #113442) were used ...
-
bioRxiv - Molecular Biology 2020Quote: ... all the sgRNAs targeting genes of interest as well as controls were cloned into LRG2.1 (derived from U6-sgRNA-GFP, Addgene: 108098 - as described in ref (Tarumoto et al. ...
-
bioRxiv - Cancer Biology 2021Quote: ... PTEN or non-targeting-control (see Table 3) were cloned into pLKV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene ID:67974) and Cas9 expressing cell lines were transduced and selected with puromycin (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... Mice that were cre-positive received the AAV-hM3D injection (test mice) or a control virus AAV-YFP (Addgene) (control mice ...
-
bioRxiv - Molecular Biology 2022Quote: ... Addgene plasmid # 12456) and 5 ng of Renilla luciferase control plasmid (a gift from David Bartel; Addgene plasmid # 12179) in each well ...
-
bioRxiv - Neuroscience 2022Quote: ... 200nl of adeno-associated virus 2 (AAV2) containing either control construct (pAAV-hSyn-EGFP; plasmid #50465; Addgene, Watertown, MA) or excitatory DREADD (pAAV-hSyn-hM3D(Gq)-mCherry ...
-
bioRxiv - Neuroscience 2022Quote: ... we injected either rAAV2/9 encoding GCaMP6s (Chen et al., 2013) under control of the CaMKII promoter (1.25 × 1013 gc/ml; AddGene viral prep #107790-AAV9 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and a non-human control were amplified from the pSB700 plasmid and cloned into PB-TRE-dCas9_VPR (Addgene #63800) using the following primers ...
-
bioRxiv - Genetics 2020Quote: ... We generated cell lines expressing KRAB-dCas9-IRES-BFP under the control of a doxycycline-inducible promoter (Addgene #85449) and the reverse tetracycline transactivator (rtTA ...
-
bioRxiv - Molecular Biology 2023Quote: Drosophila reporter plasmids expressing eGFP under the control of the inducible Metallothionein A promoter (Hy_pMT eGFP SV40; Addgene #69911), Hml promoter (Hy_pHml eGFP SV40 ...