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Citations for Addgene :
351 - 400 of 641 citations for Mouse Anti Rift Valley Fever Virus Nucleoprotein Antibody IA4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Male PV-Cre and both male and female Ai6 mice were secured in a stereotaxic frame and bilateral injections of 200-300 nl AAV1 virus vectors (AAV1-EF1a-DIO-ChETA-eYFP (Addgene: 26968) to PV-Cre mice and pENN-AAV1-hSyn-Cre-WPRE-hGH (Addgene ...
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bioRxiv - Cell Biology 2023Quote: A synthetic sequence corresponding to 2A peptide from Thoseaasigna virus capsid protein and BspTI (AflII) restriction site was first cloned to pCXLE-EGFP (Plasmid #27082, Addgene, www.addgene.org) (Okita et al. ...
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bioRxiv - Neuroscience 2023Quote: ... We expressed the soma-targeted opsin ST-ChrimsonR 43 in excitatory neurons by injecting a virus (1012 titer; AAV2/2 camKII-KV2.1-ChrimsonR-FusionRed; Addgene, plasmid #102771) into the craniotomy ...
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bioRxiv - Neuroscience 2023Quote: ... into the AC and a retrograde Cre virus: pENN/AAVrg-hSyn-Cre-WPRE-hGH (1.8E+13 vg/ml, 200nl, Addgene catalog #105553) into the pStr ...
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bioRxiv - Neuroscience 2023Quote: ... We bilaterally injected Cre-dependent DREADD virus (Roth, 2016): AAV5-hSyn-DIO-hM4D(Gi)-mCherry (2.4E+13 vg/ml, 350nl, Addgene catalog # 44362) or AAV5-hSyn-DIO-mCherry (2.6E+13 vg/ml ...
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bioRxiv - Neuroscience 2023Quote: A bicistronic virus expressing both opsin and GCaMP indicator, AAV9-hSyn-DIO-jGCaMP8s-P2A-stChrimsonR (LaFosse et al., 2023) (Addgene, 174007) was diluted in phosphate-buffered saline (final titer ...
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bioRxiv - Neuroscience 2024Quote: ... 200 μL of the viral cocktail AAV5-CamKIIa-C1V1(E122T/E162T)-TS-eYFP-WPRE-hGH (2.5 × 1012 virus molecules/mL; Penn Vector Core, University of Pennsylvania, PA, USA, Addgene number: 35499) was administered across four sites into the primary somatosensory (S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... B7GG cells were transfected by Lipofectamine 3000 (Thermo Fischer) with rabies virus genomic vectors RabV CVS-N2cΔG-eGFP (Addgene plasmid #73461) or SAD-B19ΔG-eGFP (modified from Addgene plasmid # 32634) ...
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bioRxiv - Microbiology 2020Quote: ... and the matrix protein from vesicular stomatitis virus was amplified from pVSV eGFP dG (a gift from Connie Cepko; Addgene plasmid #31842) as described above using the primers listed in Table S2 ...
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bioRxiv - Microbiology 2021Quote: The Tobacco mosaic virus (TMV) expression vector pJL-TRBO has been previously described [57] and was a gift from John Lindbo (Addgene plasmid # 80082). The TMV vector containing p26:GFP has also been previously described [41] ...
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bioRxiv - Immunology 2019Quote: ... We used the resulting virus particles to transduce immortalized wild-type C57BL/6 cells that express doxycycline-inducible SpCas9 enzyme (generated using Addgene plasmid #50661). We cultured transduced cells in 3.0 μg/ml blasticidin (Invivogen ...
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bioRxiv - Genomics 2022Quote: ... BPTF BD with an N-terminal 6xHistidine (6His) tag and Tobacco Etch Virus (TEV) Protease cleavage site was from Addgene (plasmid 39111). This was modified using the Q5 Site-directed mutagenesis kit (New England Biolabs [NEB] ...
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bioRxiv - Neuroscience 2022Quote: ... into the OB and the retrograde virus AAVrg-CamKIIα-mCherry (80 µl at titer ≥ 7×10¹² vg/mL, #114469-AAVrg, Addgene, MA, USA) into the HP ...
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bioRxiv - Neuroscience 2021Quote: ... the adeno-associated virus AAV2/1-Syn-FLEX-mRuby2-CSG-P2A-GCaMP6m-WPRE-SV40 (titer: 2.9 x 1013 GC per ml, Addgene accession no. 102816) in combination with AAV2/1.CamKII0.4.Cre.SV40 (titer ...
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bioRxiv - Cell Biology 2019Quote: ... Tetracycline-inducible MYH12A WT and S1AS2A-mutant IA32 cell lines were made by first preparing a stable rtTA-expressing IA32 cells using pLenti-CMV-rtTA3-hygro lentivirus infection (virus made with Addgene plasmid 26730) and selection with 100 μg/mL hygromycin ...
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bioRxiv - Bioengineering 2019Quote: ... H9 GFP hESC line was generated by infecting H9 hESCs with pLenti PGK GFP Puro virus generated from the plasmid purchased from AddGene (Cat#: 19070)(Campeau et al. ...
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bioRxiv - Pathology 2020Quote: ... Lentiviral vector pLV-mCherry and vesicular stomatitis virus glycoprotein (VSV-G) expression vector pMD2.G were obtained from Addgene (Watertown, MA, USA). Coding sequence of SARS-CoV-2 S gene (GenBank ...
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bioRxiv - Neuroscience 2021Quote: The CVS N2cdG-H2B-EGFP rabies virus was generated by replacing GFP in the rabies genomic plasmid RabV CVS-N2c(deltaG)-EGFP (Addgene, Plasmid #73461) with H2B-EGFP flanked by 5’ XmaI and 3’ NheI-KasI sites ...
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bioRxiv - Neuroscience 2021Quote: The SAD B19dG-H2B-EGFP virus was generated by inserting the H2B-EGFP sequence into the gG locus of the pSADdeltaG-F3 plasmid (Addgene, Plasmid #32634). The EnvA SAD B19dG-H2B-EGFP rabies viruses were generated from the genomic plasmid as described previously2 ...
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bioRxiv - Neuroscience 2020Quote: ... Non-Cre-dependent expression of hM4D(Gi) was mediated by administration of an AAV8-CaMKIIa-hM4D(Gi)-mCherry virus (Addgene; plasmid #50477) while that of a GFP reporter by administration of an AAV5-CaMKII-GFP virus (Boyden ...
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bioRxiv - Immunology 2022Quote: ... the cells were transduced with virus produced using the lentiCas9-EGFP plasmid (a gift from Phil Sharp & Feng Zhang, Addgene plasmid # 63592), single-cell sorted using a Sony SH800 cell sorter ...
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bioRxiv - Cell Biology 2022Quote: Rac1 activation was achieved by expressing PA-Rac1 via retro-virus infection using pBabe-TetCMV-puro-mCherry-PA-Rac1 (Addgene Plasmid #22035) in MV3 cells ...
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bioRxiv - Neuroscience 2023Quote: ... were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2) or a GFAP-GFP Adeno-associated virus (AAV) construct (Addgene plasmid #50473) for use in animal experiments (utilized in Figure 3 and Figure 4) ...
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bioRxiv - Immunology 2022Quote: ... Virus was generated by transfecting the sgRNA plasmid constructs into HEK 293T cells with the psPAX2 packaging (Didier Trono, Addgene plasmid #12260) and pCMV-VSVG envelope (Bob Weinberg ...
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bioRxiv - Neuroscience 2023Quote: ... For DREADD experiments 6-7 week old mice were injected bilaterally with the inhibitory virus AAV-hSyn-hM4D(Gi)-mCherry (AddGene, 50475-AAV2) or control virus AAV-hSyn-EYFP (AddGene ...
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bioRxiv - Neuroscience 2023Quote: ... or left IC (0.9 mm caudal and 1 mm lateral from lambda suture) to inject 100-200 nL of pAAV-Syn-Chronos-GFP virus (Addgene #59170-AAV1). At the end of the surgery ...
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bioRxiv - Cancer Biology 2023Quote: ... or pLenti-PGK-tdTomato-Akaluc (TdT-AkaLuc) virus (subcloned from pLenti-PGK-Venus-Akaluc(neo)) and HR180-LGR5-iCT plasmids through Gibson assembly (Addgene 124701; 129094). For cecal injections ...
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bioRxiv - Neuroscience 2023Quote: ... Two small holes were made in the skull above the injection sites and 200nl (100nl/min) of AAV8-hSyn-DIO-mCherry or AAV8- hSyn-DIO-hm4Di-mCherry virus (Addgene, Watertown, MA) was infused in both hemispheres of the PVN using a 1µL Hamilton syringe (0° angle ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth, Addgene, Watertown, MA) into the LC ...
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bioRxiv - Neuroscience 2022Quote: ... 400nl of a Cre-expressing virus that is retrogradely transported back one synapse (AAV-pmSyn1-EBFP-Cre, titer 1.00 x 1013, Addgene 51507-AAVrg, lot 27021) was injected into external segment of globus pallidus (GPe ...
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bioRxiv - Neuroscience 2021Quote: Pyramidal cell imaging experiments were performed by injecting a recombinant adeno-associated virus (rAAV) encoding GCaMP6f (rAAV1-Syn-GCaMP6f-WPRE-SV40, Addgene/Penn Vector Core) into male wild-type mice.
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bioRxiv - Neuroscience 2020Quote: ... A small (~0.5 mm) craniotomy was made and the virus AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6s.WPRSE.SV40 (Addgene 50942, titer 1.9 × 1013 vg/ml, −50 nL) was injected into the hippocampus (from bregma ...
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bioRxiv - Neuroscience 2022Quote: ... one group of animals (n=6) received a bilateral injection of a virus driving expression of the calcium indicator GCaMP7s (AAV9-hSyn-GCaMP7s-WPRE; Addgene; 300nL per side) targeting the CA1v at the following stereotaxic coordinates ...
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bioRxiv - Neuroscience 2022Quote: ... ACS-4500) using Lipofectamine 2000 in FBS free Optimem together with the virus packaging plasmids (psPAX2 and pMD2-G, Addgene # 12260 and # 12259) and the plasmid expressing either wild-type U4atac or an Empty Vector ...
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bioRxiv - Developmental Biology 2023Quote: ... a total of 9.45 μg of each library plasmids combined with 6.75 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmids 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Genetics 2023Quote: ... a total of 13.6 μg core enhancer perturbation library plasmids with 5.44 μg lentiviral packaging vector psPAX2 and 1.36 μg vesicular stomatitis virus G (VSV-G) envelope expressing plasmid pMD2.G (Addgene plasmid 12260 and 12259) were transfected with the JetPRIME (VMR ...
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bioRxiv - Neuroscience 2022Quote: ... mice were first injected with a helper virus (AAV5- hSyn-FLEX-splitTVA-2A-mCherry-2A-B19G; produced in-house, modified after Addgene plasmid no. 52473) into MEC ...
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bioRxiv - Neuroscience 2023Quote: ... typical dose 6.0×108 gc/mL) and a reporter virus (AAV-PHP-eB_7x-TRE-tdTomato, typical dose 1.8×1011 gc/mL) (Addgene plasmid id: #191210 and, #191207). The viral titers of the tTA virus used were empirically adjusted based on the Cre driver line to yield sparsely labeled brains ...
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bioRxiv - Neuroscience 2024Quote: ... DREADD+ group) was bilaterally injected in the dDG with pAAV5-CaMKIIa-hM4D(Gi)-mCherry (virus titer ≥ 3×1012 vg/ml, Addgene, North Carolina, USA). Inhibitory DREADDs are activated by the artificial ligand clozapine-N-oxide (CNO ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse TRIM21 (Addgene #105516) and CRY2Clust (Park et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse Ebf1 cDNA (Addgene) was cloned into pLVX Tet-One Puro plasmid (Clontech) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mouse Gqα15 (Addgene #40753) (Offermanns and Simon ...
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bioRxiv - Neuroscience 2022Quote: ... Err2VP16 was generated by fusing the open reading frame for the herpes simplex virus-1 (HSV-1) VP16 (amplified from pActPL-VP16AD plasmid, Addgene plasmid #15305, Watertown, USA) activation domain to Err2 ...
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bioRxiv - Neuroscience 2023Quote: We performed an initial calibrating analysis on Emx-Cre mice (see Animals) expressing Cre-dependent GFP virus (Addgene pCAG-FLEX-EGFP-WPRE; #51502-AAV9) following a dot-wise application of 1:2000 diluted virus (see Organotypic Slice Culture methods) ...
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bioRxiv - Bioengineering 2023Quote: ... was transfected with 1 µL AAV1-hSyn1-GCaMP6f-P2A-nls-dTomato virus (Addgene viral prep #51085-AAV1, http://n2t.net/addgene:51085, RRID:Addgene 51085, a gift from Jonathan Ting) diluted 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Each mouse received bilateral injections (15 nl/ hemisphere at a rate of 15 nl/min) of either excitatory DREADD virus pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene Cat# 50476-AAV5, titer: 1.7x1013) or control virus pAAV-CaMKIIa-EGFP (Addgene Cat# 50469-AAV5 ...
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bioRxiv - Systems Biology 2020Quote: ... we indirectly titered each experimental replicate’s batch of virus by co-transducing parallel samples of HCFs with pMXs-DsRed Express (Addgene 22724; a gift from Shinya Yamanaka) and pMXs-TurboGFP (see “Cloning” above ...
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bioRxiv - Neuroscience 2023Quote: Cre-dependent recombinant adeno-associated virus (rAAV) expressing GCaMP7f under the control of the Synapsin promoter (rAAV1-Syn-FLEX-jGCaMP7f-WPRE-Sv40, Addgene #104492, titer: 1 × 1013 vg/mL) was used to express GCaMP7f in interneurons.
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bioRxiv - Cancer Biology 2023Quote: Retroviral vectors expressing the cDNA of wild-type c-Myc and GFP in the murine stem cell virus backbone were purchased from Addgene (MSCV-Myc-IRES-GFP, Plasmid #18770). Doxycycline-inducible constructs were obtained by cloning c-Myc cDNA into GC385-S backbone ...
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bioRxiv - Neuroscience 2024Quote: ... We injected in that region 3x750nL of an adeno-associated virus (AAV) mix of AAV1.Syn.GCaMP (6m: Addgene 100841 or 7f: Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.Chrimson.tdTomato (UNC Vector Core ...