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Citations for Addgene :
51 - 100 of 379 citations for Mouse Anti MERS Coronavirus Spike S1 Antibody 3872 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid #145032) (76) ...
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bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032 ...
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bioRxiv - Immunology 2021Quote: Plasmid encoding spike HexaPro (designated “S” throughout) was procured from Addgene (#154754) and transfected into Expi293F™ cells (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: A plasmid encoding the SARS-CoV-2 Spike was obtained from Addgene #145032 ...
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bioRxiv - Developmental Biology 2022Quote: ... The guide sequence (r4g9, Table S1) was cloned into pX459 vector from Addgene (#62988) and 8ug of the vector was used for mESC transfection ...
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bioRxiv - Genomics 2023Quote: ... oligos containing the CRISPR sequence (table S1) were inserted into pWS082 (Addgene plasmid #90516), the template plasmid of sgRNA cassette ...
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bioRxiv - Molecular Biology 2022Quote: ... pcDNA3.1-SARS2-Spike was a gift from Dr Fang Li (Addgene plasmid # 145032); pcDNA3.1-ACE2-GFP was a gift from Dr Utpal Pajvani (Addgene plasmid # 154962) ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 expressing different spike genes or VSV-G (pMD2.G (Addgene #12259)) using Fugene®HD tranfection reagent (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... Expression constructs for various cytoskeleton or organelle marker proteins were obtained from Addgene (S1 Table). For bacterial expression of His6-tagged CAHS proteins ...
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bioRxiv - Developmental Biology 2021Quote: sgRNA and shRNA oligonucleotides (Table S1) were cloned into lentiCRISPR v2 (Addgene plasmid no. #52961) and into pSMP vector (Addgene plasmid no ...
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bioRxiv - Microbiology 2021Quote: The Plasmid expressing SARS CoV-2 Spike protein (Wuhan isolate) was procured from Addgene with 19 nineteen amino acids deletion at C- terminal that enables efficient lentiviral packaging ...
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bioRxiv - Immunology 2022Quote: ... The SARS-CoV spike expression vector was a gift from David Nemazee (Addgene #170447).
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bioRxiv - Immunology 2021Quote: The mammalian expression plasmid containing SARS-CoV-2 HexaPro spike was obtained from Addgene (Addgene plasmid # 154754 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding ancestral and variant SARS-CoV-2 spike protein were purchased from Addgene (170442 ...
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bioRxiv - Molecular Biology 2021Quote: ... Other plasmids used (for details see excel file S1): pLenti Lifeact-iRFP670 BlastR43 (Addgene Plasmid #84385); pGIZ-PuroR-IRES-GFP_shRNA anti Rab11a/b (Dharmacon) ...
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bioRxiv - Microbiology 2021Quote: ... pVAX1-SARS2-S1/S2-mut and pVAX1-SARS2-S_D614G) or VSV-G (pMD2.G Addgene #12259). The cell culture supernatants were harvested 24-72 h post transfection followed by addition of fresh media and again after 48-72h ...
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bioRxiv - Molecular Biology 2021Quote: ... or MGMT (Supplementary Table S1) were annealed and ligated into BsmBI-linearized lentiCRISPRv2 vector (Addgene, #52961). Other plasmids have been described previously (8,17) ...
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bioRxiv - Microbiology 2023Quote: pT7-S1/HER2nhd (no head domain) was constructed using pBacT7S1-T3D (Addgene, (Kobayashi et al., 2007) as a backbone plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... 27μg pTT5LnX-WHCoV-St19 (SARS-CoV2 Spike) and 54 μg pHIV-Luc-ZsGreen (Addgene, US) using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: The plasmid with cDNA encoding SARS-Cov-2 spike HexaPro (S) was obtained from Addgene. To express the S protein ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Cell Biology 2021Quote: ... and pCMV3 nCoV2019 Spike (D614 variant) C-FLAG Hygro was obtained from Addgene (Teddington, UK). Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G, Addgene #12259) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... we used an existing CMV-driven SARS-CoV-2 plasmid (pcDNA3.1-SARS2-Spike, Addgene 145032)(Shang et al. ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1 expressing SARS-CoV-2 spike gene or VSV-G (pMD2.G (Addgene #12259)) using VigoFect (Vigorous Biotechnology) ...
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bioRxiv - Immunology 2021Quote: ... with pcDNa3.1 vector expressing SARS-CoV-2 spike (BEI repository) and the helper plasmid pSPAX2 (Addgene). The VSV-G and empty lentiviruses were produced by replacing pCDNA3.1-Spike with pcDNA3.1-VSV-G or pCDNA3.1 empty vector ...
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bioRxiv - Cell Biology 2022Quote: ... pcDNA3.1-SARS2-Spike (2) and pcDNA3.1-hACE2 (2) were obtained from Addgene (Addgene plasmids #145032; #145033); pCI-SARS2-Spike expressing a codon optimized version of the gene coding for S protein of SARS-CoV-2 was provided by Nicolas Escriou (Institut Pasteur) ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 whole spike protein HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754). SARS-CoV-2 HexaPro was expressed in Expi293F cells and purified with Ni-NTA resin followed by size exclusion as described previously [61] ...
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bioRxiv - Microbiology 2022Quote: ... and the SLC35A1 gene were designed using CRISPR direct (https://crispr.dbcls.jp/; S1 Table) and each sequence was cloned into plentiCRISPR plasmids [37] (Addgene plasmid #52961 ...
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bioRxiv - Cancer Biology 2023Quote: ... using pLenti_mENPP1_fwd and pLenti_mENPP1_rev primers in Table S1 and inserted into the XbaI-BamHI sites of pLenti-CMV-GFP-Puro (Addgene). To clone pLenti-CMV-mENPP1-T238A-GFP-Puro plasmid ...
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bioRxiv - Biochemistry 2020Quote: ... anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.5 μg of SARS-CoV-2 Spike plasmid (a gift from Fang Li, Addgene plasmid #145032) using Lipofectamine 3000 transfection reagent (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... pCMV-SCoV-2S (Spike envelope plasmid; Sinobiologicals – Cat #: VG40589-UT) and pLJM1-EGFP (reporter; Addgene plasmid #19319) in a ratio of 1:1:2 respectively ...
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bioRxiv - Genetics 2020Quote: ... we co-transfected pcDNA3.1-SARS2-Spike or pcDNA3.1-SARS2-SpikeD614G (see above) with pcDNA3.1-ACE2 (Addgene 1786) at a 1:1 ratio (Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The Spike protein from pcDNA3.1-SARS2-Spike was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032);34 this gene was cloned into a modified pcDNA 3.1 backbone (Clontech-Takara ...
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bioRxiv - Microbiology 2021Quote: ... and pcDNA3.1-SARS2-Spike -a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032).
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV S was cloned into pcDNA3.3 vector from pcDNA3.1-SARS-Spike (Addgene plasmid#145031). SARS-CoV-2 N was cloned into a pSGC-his vector as described in our previous paper [17] ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids encoding the spike glycoprotein of vesicular stomatitis virus (VSV-G) and Cas9 were obtained from Addgene (cat ...
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bioRxiv - Cell Biology 2022Quote: ... guide RNAs targeting a gene of interest (Table S1) were cloned into pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene plasmid #50946), using the BbsI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs (Table S1) were designed using an online CRISPR design tool (crispr.mit.edu) and cloned into a gRNA cloning vector (Addgene, 41824) following option B from the gRNA Synthesis Protocol(53) ...
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bioRxiv - Neuroscience 2022Quote: ... we used stereotactically targeted virus injections of rAAV S1 FLEX-CAG-jGCaMP7s-WPRE (Lot v28549, Addgene, #104495 AAV-1) into the MSDB of 3— 6 months old ChAT-Cre mice ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding oligonucleotides (Table S1) were annealed and cloned in the pU6-BbsI-chiRNA vector linearized with BbsI (Addgene plasmid #45946 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA of APLF full length and APLFΔFHA and APLFΔAD were amplified with respective primer pairs (Table S1) and subcloned into Flag-HA-pCDNA3.1 (Addgene #52535) (Horn et al. ...
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bioRxiv - Plant Biology 2024Quote: ... S1) targeting the OsNAC5 gene was designed and cloned into the pRGEB31 CRISPR/Cas9 vector (Addgene, Watertown, MA, USA) (Xie and Yang ...
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bioRxiv - Neuroscience 2021Quote: ... The pcDNA3.1-SARS2-Spike plasmid was a gift from Fang Li (Addgene plasmid # 145032; http://n2t.net/addgene:145032; RRID:Addgene_145032) 39 ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenised and barcoded spike sequence templates were then added into MluI and XbaI digested lentivirus backbone (Addgene #204579) using HiFi reaction (NEB E2621L) ...
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bioRxiv - Genomics 2019Quote: ... A guide RNA sequence with a suitable protospacer adjacent motif (PAM) was found nearby the excision site (Figure 1A, Table S1) and cloned into gRNA_Cloning Vector (Addgene 41824)17 ...
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bioRxiv - Genomics 2019Quote: ... PCR products corresponding to each crRNA were amplified with U6 forward primer and corresponding antisense oligos (as listed in Supplemental Table S1) from the pX330 plasmid (Cat. 42230, Addgene). After digestion with DpnI (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... Briefly, single guide RNAs (sgRNAs, designed using crispr.mit.edu, listed in Table S1) were cloned into pX458 vector (Addgene plasmid #48138) and transfected into H1299 cells ...