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Citations for Addgene :
151 - 200 of 407 citations for Kanamycin kinase type II NEO Klebsiella pneumoniae since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... WT and mutant constructs were subsequently cloned into the vector pLenti CMV Neo DEST (705-1) (Addgene, #17392) by the GATEWAY cloning system ...
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bioRxiv - Molecular Biology 2024Quote: ... pLX209-neo-WRN K577M were a gift from Francisca Vazquez (respectively, Addgene plasmids # 125788, http://n2t.net/addgene:125788; RRID:Addgene_125788 ...
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bioRxiv - Cell Biology 2022Quote: Wild type (WT) VCP (Addgene #23971), A232E VCP (Addgene #23973) ...
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bioRxiv - Microbiology 2023Quote: ... and pBTK401 (Type 8) (Addgene_65109, Addgene_183127 ...
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bioRxiv - Neuroscience 2023Quote: Wild-type α-syn (Addgene, 3604628) was expressed and harvested from Escherichia coli as described previously29 ...
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bioRxiv - Cell Biology 2020Quote: ... GST-Snf1 kinase domain (Snf1-KD) expression vector was purchased from Addgene (#52683). Those DNA constructs were transformed in Rosetta2 (Novagen ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg pSMPUW-IRIS-Neo-H2BmRFP (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Genomics 2020Quote: ... Mutagenesis was performed via either an inducible Cas9-expressing OCI-AML3 cell line (Lenti-iCas9-neo vector; Addgene 85400) with lentiviral sgRNA expression (Addgene 70683) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cells used for RNA sequencing were first integrated with Not1-linearized pBABE-neo-hTERT plasmid (Addgene plasmid # 1774) and selected with G418 ...
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bioRxiv - Cancer Biology 2020Quote: ... pLenti CMV rtTA3 Blast (w756-1) and pLenti CMVTRE3G eGFP Neo (w821-1) were gifts from Eric Campeau (Addgene plasmids #26429 and #27569 ...
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bioRxiv - Cancer Biology 2020Quote: ... and pLenti CMVTRE3G eGFP Neo (w821-1) were gifts from Eric Campeau (Addgene plasmids #26429 and #27569; RRIDs: Addgene_26429 and Addgene_27569). BCL2L1 (Bcl-xL ...
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bioRxiv - Cancer Biology 2020Quote: MOLM-13-Cas9 cells were first transduced with a luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621) vector ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-T/t antigens expressing lentiviruses using vectors pLenti CMV-RasV12-Neo (w108-1) (HRAS G12V, #22259, Addgene), and pLenti-CMV/TO-SV40 small + Large T (w612-1 ...
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bioRxiv - Genomics 2021Quote: ... The plasmid pCI-neo beta catenin S33Y was a gift from Bert Vogelstein (Addgene plasmid # 16519; http://n2t.net/addgene:16519; RRID: Addgene_l6519).
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bioRxiv - Cancer Biology 2021Quote: ... pCW57-MCS1-P2A-MCS2 (Neo) was a gift from Adam Karpf (Addgene plasmid #89180; http://n2t.net/addgene:89180; RRID: Addgene_89180). ppyCAG_RNaseH1_WT was a gift from Xiang-Dong Fu (Addgene plasmid #111906 ...
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bioRxiv - Genomics 2022Quote: ... the donor plasmid was engineered by replacing the NeoR of Lenti-iCas9-Neo (Cao et al., 2016, Addgene #85400) with BSD ...
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bioRxiv - Cell Biology 2022Quote: ... The pSLIK-Neo-FLAG-hBirA*-GEMC1/MCIDAS plasmids were co-transfected with lentiviral packaging plasmid vectors REV (Addgene 12253)24 ...
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bioRxiv - Cancer Biology 2023Quote: ... 293FT cells were co-transfected with the Lenti-iCas9-neo vector (Addgene plasmid #85400, a gift from Qin Yan) (Cao et al ...
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bioRxiv - Systems Biology 2022Quote: ... kanamycin resistance cassettes were generated via PCR – ‘KO’ primers with 50 bp homologous arms are listed in Supplementary Table S4 – using the kanamycin (Km) cassette from pKD4 (pKD4 was a gift from Barry L. Wanner (Addgene plasmid # 45605 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The removal of the kanamycin resistance cassette before each transduction was done using the pE-FLP plasmid (Addgene plasmid #45978), pE-FLP was a gift from Drew Endy & Keith Shearwinand ...
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bioRxiv - Plant Biology 2022Quote: ... The common pUC19 origin and kanamycin (kan) marker used in all the pMIN vectors was sourced from pLSU4mg10 (Addgene #126079)27 ...
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bioRxiv - Biochemistry 2023Quote: Dephosphorylated AurA (residues 122-403, TEV-cleavable, N-terminal His6-tagged, kanamycin-resistance) in pET28a and LPP (#79748) from Addgene were cotransformed in BL21(DE3 ...
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bioRxiv - Microbiology 2023Quote: ... The “Ultra” promoter from the pUltra series of plasmids (that confer kanamycin resistance (27)) was used to generate pJC-Ultra plasmid expressing mTurquoise2 (from pLifeAct-mTurquoise2; Addgene plasmid #36201 ...
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bioRxiv - Microbiology 2023Quote: ... and pBTK401 (Type 8) (Addgene_65109, Addgene_183127, Addgene_65179 ...
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bioRxiv - Genetics 2021Quote: ... For the effector plasmids (pPB_TRE3G::ScFv-GFP-KRAB_EF1a::Neo and pPB_TRE3G::ScFv-GFP-3a3L_EF1a::Neo) the GCN4 specific scFv domain and the sfGFP gene were amplified from PlatTET-gRNA2 plasmid (Addgene #82559) and fused in frame with the human ZNF10 KRAB domain (amplified from the pAAVS1-NDi-CRISPRi (Addgene #73498) ...
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bioRxiv - Bioengineering 2022Quote: ... sgRNA cassette) were PCR-amplified and recloned into 2 lentiviral plasmids (pLKO.1 neo, Addgene #13425; pLJM-EGFP, Addgene #19319) and ...
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bioRxiv - Cell Biology 2021Quote: ... and immortalized by the viral delivery of telomerase gene using pBABE-neo-hTERT (36) (gift from Bob Weinberg, 1774, Addgene). The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Cell lines for use in orthotopic in vivo experiments were labeled with pLenti-PGK-V5-LUC Neo (Addgene plasmid #21471). The 293 cells were seeded in 10-cm-diameter dishes and transfected with pCMV-dR8.2 dvpr (Addgene plasmid #8455) ...
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bioRxiv - Cancer Biology 2021Quote: ... the annealed oligos were ligated into plentiGuide-sgRNA-P2A-mCherry-Puro50 for all experiments in SCLC and AML cell lines or lentiCRISPR V2-Neo (Addgene #52961 where the puromycin resistance gene was replaced with the G418 resistance gene ...
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bioRxiv - Microbiology 2022Quote: ... MGAT1/C1GALT1 DKO cells were used to generate MGAT1/C1GALT1/UGCG TKO cells with the lentCRISPRv2 neo vector (#98292, Addgene). Primers for sgRNA target sites used here are listed in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... CAFs were immortalized with stable expression of human telomerase reverse transcriptase (pBABE-neo-hTERT was a gift from Bob Weinberg (Addgene plasmid # 1774 ...
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bioRxiv - Microbiology 2023Quote: ... the V5-tag sequence followed by a Stop codon was introduced upstream of the IRES in the pLVX-IRES-Neo vector (Clontech, Addgene), using annealed oligo-cloning ...
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bioRxiv - Synthetic Biology 2022Quote: ... The vector backbones were amplified from plasmids carrying an RK2 origin of replication with a Kanamycin resistance gene for pKSh2 (Addgene #167515) or Gentamicin for pKSh5 (Addgene #149463) ...
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bioRxiv - Plant Biology 2023Quote: ... The final construct was assembled by combining the two Level 1 sgRNA cassettes with cassettes for resistance to kanamycin pICSL11024 (Addgene#51144) and constitutive expression of SpCas9 (pEPQD1CB0001 ...
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bioRxiv - Cell Biology 2021Quote: The pFHL-plasmid for dual expression was constructed using four DNA fragments: (i) the Kanamycin resistance gene and ColE1 origen of replication from a C1 plasmid (Addgene plasmid #54842), followed by (ii ...
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bioRxiv - Cancer Biology 2021Quote: ... MOLM-13/Cas9+ cell line stably expressing luciferase was established via lentiviral infection with a Lenti-luciferase-P2A-Neo (Addgene # 105621) vector followed by G418 (1 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: These constructs were subcloned via PCR from the corresponding squ-mNeongreen-RhoGEF2 full length constructs into Ac5-Stable2-neo (Rose Barrio and James Sutherland60, Addgene 32426).
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bioRxiv - Cell Biology 2021Quote: ... To combine multiple GOI we also used the lentiviral vector EF1a-Hygro/Neo a gift from Tobias Meyer (Addgene plasmid # 85134). Briefly ...
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bioRxiv - Biophysics 2020Quote: ... A homozygous clone that passed all QC (U2OS HP1⍺ 4) was co-transfected with the transposon vector pEF1a-OsTIR-IRES-NEO-pA-T2BH (Addgene 127910) and SB100X in pCAG globin pA (Addgene 127909) ...
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bioRxiv - Cell Biology 2022Quote: ... was synthesized and inserted into the multicistronic vector pAc5 STABLE2 Neo, which contains a GFP cassette (Gonzalez et al., 2011) (Addgene #32426), by Gibson assembly to generate pAc5-GFP:T2A:HsSPAKD210A ...
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bioRxiv - Molecular Biology 2020Quote: ... Guide RNA against Thap1(see Table S3 for sgRNA sequence) was cloned into LentiCRISPRv2 with a Neo selection marker (Addgene #98292). Brca2Y3308X mES cells 67 were grown on 0.1% gelatin (Sigma ...
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bioRxiv - Genetics 2021Quote: ... we used site-directed mutagenesis of a GFP expression plasmid (MSCV-miRE-shRNA IFT88-PGK-neo-IRES-GFP plasmid, Addgene # 73576), and substituted a single G for a C to introduce an in frame stop codon (TAG ...
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bioRxiv - Synthetic Biology 2020Quote: ... Three elements of the reporter were amplified from the following sources: five TetO binding sites upstream of a pEF promoter from PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins (Addgene #78099), TagRFP-T from pEN_ERK.KTR-tagRFP-T ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting vector containing a PGK-Neo drug-resistance cassette flanked by the HPRT homology arms was constructed by Gateway LR reaction using pENTR-L1-PGK-Neo-L2 (Addgene #141007), pUC-DEST-R3R4(R ...
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bioRxiv - Genomics 2020Quote: ... The VP64 cassette was replaced by CTCF sequences to generate dCas9-CTCF and neomycin resistant marker that was taken from pAC95-pmax-dCas9VP160-2A-neo (a gift from Rudolf Jaenisch, Addgene 48227) was inserted ...
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bioRxiv - Developmental Biology 2023Quote: Donor Plasmids for insertion of 5xtetO-pEF1-H2B-CITRINE reporter into the genome were constructed by subcloning 5xtetO-pEF1-H2B-CITRINE-PolyA from PhiC31-Neo-ins-5xtetO-pEF-H2B-Citrine-ins (Addgene# 78099)14 with right and left homology arms (500 bps each ...
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bioRxiv - Cancer Biology 2023Quote: ... as described previously.16,49 Sting knockdown in the MSI MC38 CRC cells was generated as described previously using shRNA and the pLKO.1 system.16,50 Ovalbumin (OVA) expressing MSI and CIN MC38 CRC cells were made by transfection with the pCI-neo-cOVA plasmid (Addgene #25097) and selection with 200 µg/ml G418.51
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bioRxiv - Molecular Biology 2023Quote: A fragment containing an inducible TRE-tight promoter and a LAP tag (a FLAG-tag followed by an EmGFP-tag) was inserted at the XhoI site of the plasmid pBSKDB-CAG-rtTA2sM2-IRES-tSkid-IRES-Neo (Addgene #62346), thereby generating the vector pGEH_ind_LAP-C ...
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bioRxiv - Cell Biology 2023Quote: pLVX-EF1a-H2B-emiRFP703-neo was created by PCR of H2B-emiRFP703 from pH2B-emiRP703 (a gift from Vladislav Verkhusha, AddGene #136567) with forward primer (with prepended NotI digest site ...
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bioRxiv - Cancer Biology 2023Quote: ... Control mice underwent HDTVi of a DNA mixture prepared as described above with scramble empty vectors publicly available: pT3-EF1-Neo-GFP (Addgene 69134), and the pX330-CBh- hSpCas9 (Addgene 42230 ...