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Citations for Addgene :
301 - 350 of 2784 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: Expression of N-terminally acetylated human αS and βS proteins was performed via co-expression with pNatB plasmid (Addgene #53613) in E ...
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bioRxiv - Cell Biology 2021Quote: ... and GFP-hRab31.dn3 (#1019) plasmids encoding GFP-labeled versions of the indicated human wild-type proteins were from Addgene (Cat# 49549 ...
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bioRxiv - Neuroscience 2023Quote: ... An AAV8 encoding the inhibitory designer receptor KORD fused to the fluorescent protein mCitrine under the control of the human synapsin promoter in a Cre-dependent manner was obtained from Addgene (AAV8-hSyn-dF-HA-KORD-IRES-mCitrine ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the Cre enzyme fused to the green fluorescent protein (GFP) under the control of the human synapsin promoter was obtained from Addgene (AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40 ...
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bioRxiv - Bioengineering 2021Quote: ... and B-GECO was also obtained from Addgene. MaLionG and mitoMaLionR were generated in the author’s group (T ...
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bioRxiv - Microbiology 2023Quote: ... The human ANP32A 1-149 construct was a gift from Cynthia Wolberger (Addgene plasmid # 67241 ...
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bioRxiv - Genetics 2021Quote: Mice were injected with Cre-expressing adeno-associated virus AAV1.hSyn.Cre.WPRE.hGH (105553-AAV1, Addgene) and GFP-expressing AAV1.hSyn.eGFP.WPRE.bGH (105539-AAV1 ...
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bioRxiv - Neuroscience 2022Quote: The commercial AAV5-hSynhM3D(Gq)-mCherry or the control virus AAV5-hSyn-mCherry (Addgene) were injected into the sciatic nerve of WT C57BL/6J mice (1μL/sciatic nerve ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transduced with the following virus: pTet-O-NGN2-puro (Addgene #52047): 0.1 μL per 5×104 cells ...
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bioRxiv - Neuroscience 2019Quote: ... A control virus (AAV5-CamKIIα-eGFP, 4.1×1012) (Cat No. 50469-AAV5; Addgene, USA) was administered into the contralateral left EC using identical parameters ...
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bioRxiv - Neuroscience 2022Quote: ... or a control virus AAV9-hSyn-eYFP (Addgene, viral titer 2.8 × 1013 vg/mL) was injected in the NAcSh of WT mice while AAV9-Syn-Flex-GcAMP6f-eYFP (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... The TauRD(P301S)-YFP plasmid DNA with virus packing pDNAs (psPAX2; pMD2.G, Addgene) was transfected into 293T cells by 1mg/mL PEI transfection reagent and incubated for 36 hours ...
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bioRxiv - Neuroscience 2023Quote: ... iAChSnFR and iAChSnFR-Null constructs and AAV virus are available from Addgene (137950–137962).
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bioRxiv - Microbiology 2023Quote: ... a vesicular stomatitis virus G (VSV-G) envelope expression vector (pMD2.G, #12259, Addgene) and a lentiviral transfer vector containing the transgene in a ratio of 2:1:3 by calcium phosphate transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... mitochondria were labelled in cells by transduction with pLV-mitoDsRed virus (Addgene, Plasmid 44386) harvested post expression in 293-FT cells and lentiviral particle production ...
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bioRxiv - Neuroscience 2023Quote: Birds were injected with the AAV9-CAG-GCaMP6f-WPRE-SV40 virus (100836-AAV9, Addgene). The total amount of virus injected was 897 nL (65 injections of 13.8 nL each) ...
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bioRxiv - Cancer Biology 2024Quote: ... the shRNA constructs were packaged into second-generation virus particles using psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Physiology 2024Quote: AAV9 virus expressing jGCaMP8m under a CAG promoter was obtained from Addgene (162381-AAV9). For injections ...
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bioRxiv - Neuroscience 2024Quote: ... commenced with stereotaxic surgery to infuse a pAAV1 CD68-hM4D(Gi)-mCherry virus (Addgene viral prep #75033-AAV1 from Bryan Roth ...
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bioRxiv - Neuroscience 2024Quote: ... Animals used for RSC imaging received a mixture of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (Addgene: 100837), AAV1.cFos-tTA and AAV1.TRE.mCherry at 20 to 120 nL/min into dorsal cortex using stereotactic coordinates −1.7 and −2.3 mm posterior to bregma ...
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bioRxiv - Cancer Biology 2024Quote: HT29 cells previously infected with pLenti-U6-tdTomatato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and TdTomato were used ...
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bioRxiv - Cell Biology 2020Quote: ... human KLF4 (RRID:Addgene_17219), human c-MYC (RRID:Addgene_17220 ...
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bioRxiv - Cell Biology 2020Quote: ... human SOX2 (RRID:Addgene_17218), human KLF4 (RRID:Addgene_17219) ...
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bioRxiv - Neuroscience 2022Quote: ... human DNAJB4 (Addgene) and DNAJB6 (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... was made by amplifying TPD54 by PCR from human Tumor protein D54 (IMAGE clone: 3446037) and inserting into pIRES-EGFP-puro (Addgene #45567) via NheI and XhoI ...
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bioRxiv - Microbiology 2022Quote: To make human ACE2 protein, pcDNA3-sACE2-WT(732)-IgG1 (Chan et al., 2020) (Addgene plasmid #154104, gift of Erik Procko) plasmid was transfected into Expi293 cells using PEI at a ratio of 1:3 ...
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bioRxiv - Biochemistry 2023Quote: ... Human ATAD2B bromodomain-containing protein (residues 953−1085, Uniprot code: Q9ULI0) was a gift from Nicole Burgess-Brown (Addgene plasmid # 39046) was PCR-amplified and cloned into pDEST15 (GlaxoSmithKline ...
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bioRxiv - Cancer Biology 2023Quote: ... the murine shRNA hairpins pLKO.1-shCdkn2a #1 (TRCN0000077816) and #2 (TRCN0000362595) and the human and murine pLKO.1-shGFP control (Addgene, cat#30323) vectors were packaged using the ViraPower Kit (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Human PARP6 (Uniprot #Q2NL67-1) was cloned into a modified pFASTBac1 vector (Addgene #30116) with N-terminal 6X His-maltose binding protein (MBP ...
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bioRxiv - Neuroscience 2023Quote: ... human TDP-43M337V has subcloned into the pLenti CMV Puro DEST (W118-1, Addgene) plasmid as previously described (Zhang et al ...
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bioRxiv - Cell Biology 2021Quote: ... CMV-GFP-NMHC II-B (Addgene plasmids # 11347, 11348)(Wei and Adelstein ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... and pSH-EFIRES-B-Seipin-miniIAA7-mEGFP (Addgene #129719) [3,10] ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were bilaterally microinjected with 500 nL of AAV1.Syn.GCaMP6f.WPRE.SV40 virus (purchased from Addgene 100837) at 20 -120 nL/min into the dorsal cortex using the stereotactic coordinates ...
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bioRxiv - Bioengineering 2021Quote: ... Lentivirus pseudotyped with the vesicular stomatitis virus spike G (pCMV-VSV-G; Addgene plasmid # 8454) was used as a positive control and the bald lentivirus having no coat was used as a negative control.
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bioRxiv - Neuroscience 2021Quote: MSCV (Murine Stem Cell Virus)-CreERT2 puro plasmid was a gift from Tyler Jacks (Addgene, Watertown ...
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bioRxiv - Cancer Biology 2020Quote: HT29 cells were infected with the pLenti-U6-tdTomato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and dTomato ...
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bioRxiv - Neuroscience 2021Quote: ... DV 1.4) before injecting 250 nL of virus (pGP-AAV1-syn-jGCaMP7f-SV40-WPRE; Addgene) in 50 nL pulses each interspersed by ∼10 s and the final pulse followed by a 10 min period where the needle was left in place to allow for diffusion ...
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bioRxiv - Physiology 2020Quote: ... A retrograde AAV virus carrying a Cre gene (AAVrg-hSyn1-Cre, Addgene; 200 nl volume) was injected into the PBN (coordinates ...
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bioRxiv - Cancer Biology 2022Quote: MB-004 cells were transduced with the enti-dCas9-KRAB-T2A-GFP virus (Addgene # 71237). sgRNAs targeting SOX11/HNRNPH1-binding motifs in the distal enhancers were designed using the CHOPCHOP program (https://chopchop.cbu.uib.no) ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5µl of virus (either AAV1-CaMKIIa-ArchT-GFP or AAV8-CaMKIIa-GFP, Addgene, MA) was infused bilaterally with a 1 µl Hamilton syringe into either the PRC (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Anxa1-iCre mice (new line) were injected with AAV1-CAG-FLEX-GCaMP6f virus (RRID: Addgene_100835). For labelling VGlut2+ or Aldh1a1+ dopamine neurons ...
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bioRxiv - Neuroscience 2023Quote: ... the pipette containing AAVPHP.S-CAG-FLEX-tdTomato virus (Addgene 28306-PHP.S, 1.8×1013 vg/mL) targeted at 2 locations in the mandibular branch spaced 250 um apart ...
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bioRxiv - Neuroscience 2023Quote: ... we infected organoids for 2 weeks with the AAV1-hSYN-eGFP virus (Addgene, 105539-AAV1), which directs expression of eGFP under the neuron-specific synapsin promoter ...
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bioRxiv - Neuroscience 2023Quote: ... virus expressing tdTomato under the PVIN specific S5E2 enhancer (AAV9-S5E2-tdTomato, Addgene 135630-AAV9) was injected in mice using coordinates ...
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bioRxiv - Neuroscience 2023Quote: Unilateral stereotaxic injections of AAV5-CaMKIIα-EGFP (Addgene #50469; virus titer ≥ 3×10¹² vg/mL) and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362 ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene #44362; virus titer ≥ 7×10¹² vg/mL) viruses were performed on adult anesthetized (isoflurane ...
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bioRxiv - Cell Biology 2021Quote: ... NANOG and PRDM14 (see Table 1) were cloned into pLentiCRISPR V2 vectors with puro (SOX2, NANOG) or hygromycin B (PRDM14) resistance (Addgene plasmids #52961 and #98291 respectively ...
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bioRxiv - Biochemistry 2021Quote: Site-directed mutagenesis was performed on KRAS 1-169 (Q61H, isoform b, a gift from Cheryl Arrowsmith, Addgene plasmid #25153) to obtain the wild-type sequence ...
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bioRxiv - Neuroscience 2023Quote: Cre-dependent recombinant adeno-associated virus (rAAV) expressing GCaMP7f under the control of the Synapsin promoter (rAAV1-Syn-FLEX-jGCaMP7f-WPRE-Sv40, Addgene #104492, titer: 1 × 1013 vg/mL) was used to express GCaMP7f in interneurons.
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bioRxiv - Neuroscience 2024Quote: ... We injected in that region 3x750nL of an adeno-associated virus (AAV) mix of AAV1.Syn.GCaMP (6m: Addgene 100841 or 7f: Addgene 104488; dilution 1:10 ∼ 1x1013 GC/mL) and AAV1.Syn.Flex.Chrimson.tdTomato (UNC Vector Core ...