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Citations for Addgene :
51 - 100 of 1666 citations for Human Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... GFP as non-human target (NHT) control (5’-GGAGCGCACCATCTTCTTCA-3’, 5’-GCCACAAGTTCAGCGTGTC-3’, and 5’-GGGCGAGGAGCTGTTCACCG-3’) cloned into pLentiCRISPRv2 (Addgene, 52961, deposited by Feng Zhang). To generate lentiviral particles ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA for human ATP6V0C (5’-GAATAGTCGGGGCTGCTGGG-3’) and ATP6V0D1 (5’-TCGATGACTGACACCGTCAG-3’) were cloned to lentiCRISPR v2 plasmid (Plasmid #52961, Addgene), followed by virus package and infection procedures as described previously69 ...
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bioRxiv - Biophysics 2020Quote: ... and a guide RNA/Cas9 plasmid pX330 human 3’ HP1α gRNA (Addgene 127907) with Lipofectamine 2000 according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... to clone each construct into a pINDUCER21 doxycycline-inducible lentiviral vector (Addgene #46948). All primers are listed in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... A Dox-inducible Cas9 lentiviral vector was modified from LT3GEPIR (Addgene plasmid 111177): T3G-GFP-(miR-E)-PGK-Puro-IRES-rtTA3 ...
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bioRxiv - Cancer Biology 2023Quote: ... cloned in a doxycycline-inducible lentiviral vector Tet-pLKO-puro vector (Addgene #21915). Daoy medulloblastoma cells were transduced with the lentiviral particles carrying the shRNA construct and stably transduced cells were selected in the presence of puromycin ...
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bioRxiv - Microbiology 2020Quote: ... miniSOG-Alpha-V-Integrin-25 (Addgene plasmid # 57763)51 and Beta1-GFP in pHcgreen donated by Martin Humphries (Addgene plasmid # 69804)52 ...
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bioRxiv - Cell Biology 2023Quote: ... which was a gift from Alpha Yap (Addgene plasmid # 71366 ...
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bioRxiv - Bioengineering 2023Quote: Full length plasmid of PI3KCA (phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit alpha, NM_006218.4) was purchased from Addgene (Plasmid ID: 81736, Hahn and Root Lab). The coding sequence of PI3KCA was cloned into Lenti-PCDH-EF1-mNeonGreen-MCS-T2A-puromycin plasmid (a kind gift from Fırat-Karalar Lab ...
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bioRxiv - Genomics 2022Quote: A549 cells expanded from a single clone harboring a doxycycline-inducible Cas9 (Addgene #114010) were a gift from J.T ...
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bioRxiv - Cancer Biology 2022Quote: ... before cloning into the lentiviral DOX-inducible destination vector pLIX403 (Addgene Cat. no. 41395) using LR Clonase II ...
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bioRxiv - Microbiology 2019Quote: ... The pC008 (pBR322 with Tet-inducible RFP) was a gift from Feng Zhang (Addgene plasmid # 79157 ...
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bioRxiv - Genetics 2020Quote: ... Each resulting construct was subcloned a doxycycline-inducible lentiviral expression plasmid (pCW57.1, Addgene #41393) by digestion with SalI and NheI and ligation with T4 DNA ligase (NEB).
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bioRxiv - Cancer Biology 2022Quote: ... to transfer the coding regions into the Tet-inducible lentiviral vector pInducer20 (#44012, Addgene). p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... Entry plasmids were recombined to the all-in-one Tet-inducible pLX402 (Addgene #25896) destination vector with Gateway LR Clonase II Enzyme Mix (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Arabinose inducible mAmetrine (pBad-mAmetrine) was a gift from Robert Campbell (Addgene plasmid # 18083). mAmetrine was inserted in place of GFP in both pDawn and pDusk plasmids via PCR and Gibson assembly (NEB HiFi mix) ...
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bioRxiv - Cell Biology 2023Quote: To make plasmid encoding inducible E2F2: The FUW-tetO-hOKMS plasmid (plasmid #51543; Addgene) was cut with EcoR1 to linearize it and remove the original OKMS insert ...
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bioRxiv - Biophysics 2023Quote: The EGFP-alpha-synuclein gene was amplified from Addgene plasmid # 40822 (EGFP-alpha-synuclein-WT was a gift from David Rubinsztein (http://n2t.net/addgene:40822 ...
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bioRxiv - Genetics 2020Quote: ... were synthesized and cloned into doxycycline-inducible lentiviral pCW57-MCS1-2A-MCS2 vector (Addgene, #71782). THP1 TREM2 KO cells were transduced with lentiviral particles expressing either of wild-type TREM2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and an inducible qtRNA expression plasmid (for example, https://benchling.com/s/seq-F5NZDNmxOhUoWp5DX41h, Addgene #134800). Bacteria were grown overnight ...
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bioRxiv - Neuroscience 2022Quote: ... AAV vectors containing Cre-inducible GCaMP6f (AAV.9.Syn.Flex.GCaMP6f.WPRE.SV40, Addgene; TIter≥2.1×13 GC/ml) were injected into the right DLS or DMS by stereotaxic surgery ...
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bioRxiv - Neuroscience 2021Quote: HEK293T cells were transfected with an inducible nuclear-localized HT protein (HT-NLS, Addgene #82518). Doxycycline (DOX ...
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bioRxiv - Cancer Biology 2023Quote: ... The lentivirus Doxycycline-inducible expression system using the pInducer20 vector was purchased from Addgene (#44012). pInducer20 constructs were cloned with KRASG12V-HA cDNA insert by HiFi Assembly (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were cloned into the doxycycline-inducible plasmid pCW57-MCS1-2A-MCS2 (Addgene #71782), which was modified by adding bGHpolyA between the MluI and BamHI restriction sites ...
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bioRxiv - Molecular Biology 2024Quote: Neurogenin 2 (NGN2) was expressed in iPSC lines using a tetracycline inducible promoter (Addgene 172115) integrated using Piggybac plasmid EFa1-Transposase (Addgene plasmid 172116 ...
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bioRxiv - Molecular Biology 2019Quote: ... we modified the vector pET21a-alpha-synuclein (Addgene plasmid 51486) by introducing a 6x-histidine tag followed by a TEV cleavage site to the N-terminal of α-Syn ...
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bioRxiv - Cell Biology 2022Quote: ... mCh-alpha-tubulin63 was a gift from Gia Voeltz (Addgene plasmid # 49149 ...
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bioRxiv - Cell Biology 2024Quote: ... or human Mon1b (5’-GATGTGCAGATGGAGGTCGG-3’) were cloned into pSpCas9(BB)-2A-Puro (PX459) (Addgene #48139). The donor constructs used for homologous recombination were generated by cloning into the pUC19 vector with two ∼600-800-nucleotide fragments of genomic DNA upstream and downstream of the start codon of human USP8 ...
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bioRxiv - Biochemistry 2024Quote: ... human PLD3 sgRNA (5′-3′) was cloned into pSpCa9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) as described (Ran et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The Cas9-T2A-mCherry and Dox-inducible sgRNA vectors were obtained from Addgene (#70182 and #70183). sgRNAs were cloned into BsmBI-digested vectors ...
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bioRxiv - Cancer Biology 2021Quote: ... A doxycycline-inducible Cas9 clonal cell line of NALM-6 (using plasmid pCW-Cas9, Addgene #50661) was generated ...
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bioRxiv - Developmental Biology 2019Quote: ... MEFs were transduced with the doxycycline-inducible mouse tet-STEMCCA [27] and rtTA lentiviruses (Addgene # 20342) at an MOI of 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... WT or Mutant FGFR2 IIIb ORFs were cloned into pInducer20 Tetracycline-inducible lentiviral vector (Addgene #44012) using gateway cloning technology (Invitrogen #11791019) ...
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bioRxiv - Genomics 2022Quote: ... Inducible knock-out cell lines were produced with the TLCV2 CRISPR-Cas9 backbone (Addgene no. 87360) that includes a doxycycline-inducible Cas9-2A-eGFP cassette ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen-inducible Rosa26::CreERT2 embryo and immortalized by transduction of pMXs-hc-MYC (Addgene 17220) followed by limiting dilution and clone derivation (see “iMEF B” in ref ...
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bioRxiv - Molecular Biology 2024Quote: Inducible Cas9 vector pCW-Cas9-blast and gRNA expression vector pLentiGuide-puro were purchased from Addgene. Oligonucleotide sequences used for gRNA cloning are available upon request ...
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bioRxiv - Immunology 2022Quote: ... for Alpha-S and pcDNA3.3-SARS2-B.1.617.2 (Addgene, no.172320) for Delta-S proteins ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-ROCK2 was a gift from Alpha Yap (Addgene plasmid # 101296)(88) ...
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bioRxiv - Cell Biology 2020Quote: GFP-mROCK2 was a gift from Alpha Yap (Addgene plasmid # 101296). 500 ng of plasmid DNA was transfected into 1×105 hTERT RPE-1 cells in DMEM/F12 medium containing 10% fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2021Quote: The pIRESneo-EGFP-alpha Tubulin plasmid was obtained from Addgene (USA) and mutation (Y224G ...
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bioRxiv - Biophysics 2022Quote: ... pCMV-mCherry-Alpha-Actinin-19 (Addgene #54975, gift from Michael Davidson), pCMV-mApple-MyosinIIA-N-18 (Addgene #54930 ...
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bioRxiv - Biophysics 2022Quote: ... pCMV-mEmerald-Alpha-Actinin-19 (Addgene #53989, gift from Michael Davidson), pCMV-mCherry-Alpha-Actinin-19 (Addgene #54975 ...
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bioRxiv - Cell Biology 2022Quote: ... The ORF of EPLIN isoform alpha was PCR amplified from Addgene plasmid #40928 to generate plasmid #1 ...
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bioRxiv - Immunology 2020Quote: ... Table 1) were cloned into the Doxycycline (Dox)-inducible FgH1t-UTG or FgH1t-UTC construct (Addgene #70183), followed by lentiviral transduction of Cas9-expressing THP-1 and HeLa cell lines ...
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bioRxiv - Cell Biology 2020Quote: The doxycycline-inducible PLK4 plasmid was generated by PCR of the wild-type PLK4 cDNA from Addgene plasmid #41165 by PfuUltra II Fusion HS DNA polymerase (Agilent #600670. ...
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bioRxiv - Cancer Biology 2021Quote: ... the tetracycline inducible expression construct was PCR-amplified from pCW57.1 (a gift from David Root, Addgene # 41393) and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs ...
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bioRxiv - Immunology 2020Quote: ... the doxycycline inducible Tet-On-3G_hPGK promoter sequence was PCR amplified from the plasmid pMK243 (Addgene, #72835) with 5’ SalI and 3’ MluI digestion sites ...
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bioRxiv - Neuroscience 2022Quote: ... As additional positive control we injected in LHA cre-inducible ChR (pAAV-Ef1a-DIO hChR2-EYFP; Addgene viral pep #35509-AAV5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Doxycycline-inducible shRNAs were established using retroviral TRMPVIR vector (kind gift from Scott Lowe, Addgene plasmid #27994) as previously described54 ...
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bioRxiv - Cancer Biology 2021Quote: The inducible BCL6 shRNA vectors were generated based on a pLVX-TetOne-Puro vector (RRID: Addgene 124797) according to standard protocols ...