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Citations for Addgene :
1 - 50 of 987 citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Biochemistry 2024Quote: ... and recombinant human GST-CK2α (Addgene #27083) enzymes were produced in-house as previously described [30] ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Synthetic Biology 2023Quote: - “Px-PhiYFP-triplex-28-M13-28-pA” (Addgene ID 202047) - contains a cloning site in place of the promoter so that targeting arrays can be introduced upstream of the non- coding transcript harboring a 28 nt-flanked gRNA cloning site in the 3’ UTR ...
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bioRxiv - Biochemistry 2024Quote: Human Drosha isoform 4 and human DGCR8 clones were purchased from Addgene. cDNA encoding different length variants of Drosha were cloned into pFL plasmid and expressed as a N-terminal Dual-strep tag fusion ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... inverse PCR from CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) using a pair of primers including SacI restriction sites generated a linear plasmid ...
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bioRxiv - Genetics 2021Quote: ... [28] and Michael Lampson (Addgene plasmid # 67764 ...
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bioRxiv - Zoology 2023Quote: ... or a human TLR4 expression clone (Addgene, cat. #13018). Additionally ...
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bioRxiv - Neuroscience 2023Quote: ... For the transfection with human Pdhk1 clone (hPdhk1; Addgene: 20564). Pre-OLs after partial differentiation for 4 days from the OPCs ...
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bioRxiv - Cell Biology 2020Quote: ... h-lin-28 and EBNA-1 (Addgene plasmids #27077 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we designed a construct for the first tier of the dCas9-based cascade that is expressed from the EF1α promoter and contains a SapI-flanked cloning site in the 3’UTR for adding a gRNA to target a downstream target: “EF1a-Triplex-28-M13-28-pA” (Addgene ID 202041). Detailed protocols for modifying all of these constructs are provided on the Addgene website.
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bioRxiv - Cancer Biology 2022Quote: The Brunello library (28) was purchased from Addgene (73178). In all ...
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bioRxiv - Cancer Biology 2024Quote: ... The CRISPR-Cas9 cloning vector px459 (Addgene 62988; (28)) was a kind gift from Fred Dick ...
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bioRxiv - Cancer Biology 2023Quote: ... RRID:Addgene_52962).28 FUCas9Cherry was a gift from Marco Herold (Addgene plasmid # 70182 ...
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bioRxiv - Synthetic Biology 2023Quote: ... We then replaced the mCerulean gene carried in the new P3 plasmid first with the dsRed2 gene amplified from the CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) into the FseI-treated P3-7xTS by restriction-ligation to create P3-7xTS-dsRed2 ...
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bioRxiv - Biochemistry 2021Quote: The gene encoding human SLC7A11(I.M.A.G.E. clone IRAUp969G0966D) was cloned into pLexM (Addgene 99844) 53 with an N-terminal Flag tag inserted via PCR ...
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bioRxiv - Microbiology 2024Quote: ... tuberculosis H37Ra strain expressing pH-GFP[28] (pUV15-pHGFP; Addgene plasmid #70045 ...
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bioRxiv - Molecular Biology 2023Quote: - recombinant pGEX-4T-3-P53: The human-p53 cDNA was previously cloned in the EcoRI site of pGEX-4T3 (Addgene_79149) under the lac-trp hybrid promoter that is inducible by IPTG ...
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bioRxiv - Genetics 2021Quote: ... [28] and Michael Lampson (Addgene plasmid # 67764; http://n2t.net/addgene:67764; RRID:Addgene_67764) [50] respectively.
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding recombinant WT (RRID:Addgene_92100) and M880A mutant human GST-TAF3-PHD were kindly provided by Albert Jeltsch (Kungulovski et al. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A human elongation factor 1 (EF1) promoter was introduced using a Gateway att4-att1 Entry clone (Addgene, Watertown, MA, #162920). Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Human orfeome clone 10217) was gateway cloned into an attR-destination vector encoding an N-terminal FLAG tag (Addgene, Plasmid #18700), with the Gateway™ LR Clonase™ II Enzyme mix (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... we used the Gateway recombination system to introduce the following Myc ORF clone HsCD00039771 in pDONR221from the CCSB Human ORFeome Collection into pHAGE-TRE-DEST-NBioTAP (Addgene #53568) and pHAGE-TRE-DEST-CBioTAP lentiviral vectors (Addgene #53569) ...
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bioRxiv - Cell Biology 2020Quote: ... was made by amplifying TPD54 by PCR from human Tumor protein D54 (IMAGE clone: 3446037) and inserting into pIRES-EGFP-puro (Addgene #45567) via NheI and XhoI ...
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bioRxiv - Cancer Biology 2023Quote: ... (NM_004458) Human Tagged ORF Clone (cat# RC205356) plasmids and cloned into a 3rd generation lentiviral vector PLJM1-EGFP (Addgene, cat# 19319). The control and ERBB2 OE cells were generated using retroviral vectors pBABE-puro (Addgene ...
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bioRxiv - Genomics 2023Quote: ... The transgene was constructed using the Gateway recombination system to introduce the Myc ORF clone HsCD00039771 in pDONR221from the CCSB Human ORFeome Collection into the pHAGE-TRE-DEST-NBioTAP (Addgene #53568) lentiviral vector ...
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bioRxiv - Cancer Biology 2023Quote: ... RRID:Addgene_52962).28 FUCas9Cherry was a gift from Marco Herold (Addgene plasmid # 70182 ; http://n2t.net/addgene:70182 ; RRID:Addgene_70182).29 Lentiviruses were produced by transient transfection of 293T cells with viral plasmids along with gag- ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Bioengineering 2021Quote: ... and Tspan12 were gifts from Jeremy Nathans and the human ACE2 clone (Shang et al., 2020) was obtained from Addgene (code 145033). Synthetic DNA fragments of engineered VH containing SARS-CoV-2 RBD (resi 333-527 ...
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bioRxiv - Cancer Biology 2023Quote: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library set A and B (Addgene, 92379 and 92380) separately ...
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bioRxiv - Genetics 2022Quote: ... Wild-type clones were therefore generated by transfection of HAP1 cells with a plasmid expressing SpCas9-HF1 variant but no sgRNA (the VP12 plasmid, a gift from Keith Joung [28]; Addgene plasmid #72247; http://n2t.net/addgene:72247; RRID:Addgene_72247), and went through the same isolation and selection process as mutant clones.
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bioRxiv - Cancer Biology 2022Quote: ... human E2F5 and human DP1 were purchased from Addgene, whereas the ORF encoding human p130 from Origene.
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bioRxiv - Neuroscience 2020Quote: ... HarmPBP2 and HarmPBP3 were cloned into p10 plasmid (pJFRC-28-10-10×UAS-IVS-GFP-P10, Addgene plasmid # 36431). For phiC31 integrase-mediated P-element insertion on chromosome 2 ...
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bioRxiv - Biochemistry 2021Quote: The RAS binding domain of C-RAF kinase (RAF-RBD) (Brtva et al., 1995) and full-length human RASSF5 from the RAS clone collection were obtained from Addgene (Raf1-RBD: #13338, RASSF5: #70545). The RAS clone collection was a gift from Dominic Esposito (Addgene kit 1000000070) ...
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bioRxiv - Biophysics 2024Quote: ... the DNA fragments of human TDP-43 obtained from the TDP-43 expression plasmid as previously reported 45 and G3BP1 from Addgene (Clone#129339, Watertown, MA, USA) were inserted into the HindIII and BamHI sites of pcDNA-SNR (pTDP43-SNR and pG3BP1-SNR ...
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bioRxiv - Cell Biology 2022Quote: 6.0 × 10^6 BJAB clone B2 cells expressing both Cas9 and ERAAP dsRed were transduced with lentivirus of human GeCKO v2 library (Addgene, #1000000049, gift from Feng Zhang) at an MOI of 0.4 using spin-infection (1,000 g for 2 h at 33 °C) ...
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bioRxiv - Genetics 2022Quote: ... Wild-type clones were therefore generated by transfection of HAP1 cells with a plasmid expressing SpCas9-HF1 variant but no sgRNA (the VP12 plasmid, a gift from Keith Joung [28]; Addgene plasmid #72247 ...
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BTBD9 is a novel component of IGF signaling and regulates manganese-induced dopaminergic dysfunctionbioRxiv - Neuroscience 2021Quote: ... eft-3::hpo-9 or BTBD9 alone were co-injected into tm3719 strain with pG2M36 (myo-3::dsRed) and pBCN27-R4R3 (rpl-28::PuroR, Addgene), which were used as the selective markers for transformation ...
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bioRxiv - Biochemistry 2023Quote: E.coli codon optimized gBlocks encoding CAHS D and its mutant proteins used in this study were synthesized by (Integrated DNA Technologies) and cloned into pET-28 b (+) vector (Addgene) for bacterial expression ...
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bioRxiv - Molecular Biology 2022Quote: The sequences of PTBP1-WT and PTBP1-C23S were subcloned into pXR002 (EF1a-dCasRx-2A-EGFP 28 vector, Addgene, #109050) at restriction site BsiWI (NEB R0553S ...
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bioRxiv - Microbiology 2023Quote: ... A pair of forward and reverse oligonucleotides for the generation of each sgRNA (synthesized by IDT) were annealed by published methods (28) and were inserted into pLenti-CRISPR (Addgene) between BsmBI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... human KLF4 (RRID:Addgene_17219), human c-MYC (RRID:Addgene_17220 ...
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bioRxiv - Cell Biology 2020Quote: ... human SOX2 (RRID:Addgene_17218), human KLF4 (RRID:Addgene_17219) ...
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bioRxiv - Neuroscience 2022Quote: ... human DNAJB4 (Addgene) and DNAJB6 (Addgene ...
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bioRxiv - Biochemistry 2022Quote: Recombinant SidK1-278 encoded by plasmid pSAB35 (Addgene plasmid #175787) was expressed in E ...
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bioRxiv - Cell Biology 2024Quote: ... human Dynamin2 (#27689) and human AP2μ2 (#27672) were purchased from Addgene. cDNA encoding human AP2β2 (Clone ID ...
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bioRxiv - Immunology 2020Quote: ... GTG ACT GGC CAA GCC GTA G) were synthesised according to Sanjana et al (28) and cloned into pSpCas9(BB)-2A-GFP (Addgene, #48138) following BbsI digestion ...